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71. 发明申请

WO2009057931A2 DRIED COMPOSITION FOR HOT-START PCR WITH LONG-TERM STABILITY 审中-公开
标题翻译：用于具有长期稳定性的热启动PCR的干燥组合物
公开(公告)号：WO2009057931A2
公开(公告)日：2009-05-07
申请号：PCT/KR2008006355
申请日：2008-10-28
申请人： BIONEER CORP , KIM SEONG-YOUL , KIM HYUN BAE , PARK HAE-JOON , PARK HAN OH
发明人： KIM SEONG-YOUL , KIM HYUN BAE , PARK HAE-JOON , PARK HAN OH
IPC分类号： C12Q1/00
CPC分类号： C12Q1/686 , C12Q2549/101 , C12Q2527/125
摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long- term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.
摘要翻译：本发明涉及一种用于热启动PCR的干燥组合物，更准确地说是一种用于热启动PCR的干燥组合物，其具有改进的稳定性和长期储存性，其特征在于通过以下步骤制备反应混合物：将含有反应缓冲液，MgCl 2，4种dNTP，反应管中含有焦磷酸的DNA聚合酶和焦磷酸酶; 并干燥上述制备的反应混合物，其制备方法和使用其制备用于扩增核酸的方法。在干燥前，将热干燥的PCR干燥组合物加入焦磷酸盐和焦磷酸酶，与常规热启动用组合物相比，可以具有改善的稳定性和长期储存性以及使用方便性。因此，该组合物可以有效地用于热启动PCR，多重PCR或实时定量PCR。

72. 发明申请

WO2008115002A1 CYCLIC REVERSE TRANSCRIPTION METHOD 审中-公开
标题翻译：循环逆转录方法
公开(公告)号：WO2008115002A1
公开(公告)日：2008-09-25
申请号：PCT/KR2008/001545
申请日：2008-03-19
申请人： BIONEER CORPORATION , JEONG, Hui Jeong , KIM, Min-Jung , PARK, Hae-Joon , PARK, Han Oh
发明人： JEONG, Hui Jeong , KIM, Min-Jung , PARK, Hae-Joon , PARK, Han Oh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12Q2537/155 , C12Q2527/101 , C12Q2521/107
摘要： Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of : (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10 °C to 40 °G and, (ii) performing reaction with the resultant reaction solution at 42° to 55°C.
摘要翻译：公开了一种循环逆转录方法，其包括在一个或多个循环中进行逆转录反应，每个循环包括以下步骤：（i）与包含模板RNA，RNA依赖性DNA聚合酶的反转录反应溶液，反应缓冲液，用于逆转录的引物和dNTP，以及任选的稳定剂，在10℃至40℃下，和（ii）在42℃至55℃下与所得反应溶液进行反应。

73. 发明申请

WO2008016214A1 LACTIC ACID BACTERIA ISOLATED FROM MOTHER'S MILK WITH PROBIOTIC ACTIVITY AND INHIBITORY ACTIVITY AGAINST BODY WEIGHT AUGMENTATION 审中-公开
标题翻译：从母乳中分离的乳酸菌具有抗生素活性和抑制体重反应的活性
公开(公告)号：WO2008016214A1
公开(公告)日：2008-02-07
申请号：PCT/KR2007/002363
申请日：2007-05-14
申请人： BIONEER CORPORATION , KANG, Ji Hee , YOU, Byeung Il , YUN, Sung Il , PARK, Han Oh
发明人： KANG, Ji Hee , YOU, Byeung Il , YUN, Sung Il , PARK, Han Oh
IPC分类号： C12N1/20
CPC分类号： C12R1/225 , A23C9/1234 , A23L33/135 , A23L33/18 , A23Y2220/37 , A61K38/00 , C07K14/335 , C12N1/20
摘要： The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a Lactobacillus gasseri BNR17 strain that is isolated from Korean mother's milk and has excellent probiotic activity including acid resistance, bile acid resistance and antimicrobial activity and weight gaining inhibitory effect as well. Again, the Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body. Therefore, the strain of the invention, owing to such beneficiary effects, can be effectively used not only for the production of fermented milk, other fermented food products and animal feeds but also for the production of live cell products and food additives for preventing weight gaining.
摘要翻译：本发明涉及从人母乳分离的乳酸菌，更准确地说是从韩国母乳中分离的加氏乳杆菌BNR17菌株，具有优异的益生菌活性，包括耐酸性，胆汁酸耐性和抗微生物活性以及增重抑制作用好。此外，本发明的加氏乳杆菌BNR17除了通过从包含在食物中的单糖合成难消化的多糖而获得的重量增加抑制作用之外，还具有优异的耐酸性，耐胆汁酸性，肠吸收活性和抗病原微生物的抗微生物活性，合成多糖出体外。因此，由于这种受益效应，本发明的菌株不仅可以有效地用于生产发酵乳，其他发酵食品和动物饲料，而且可用于生产活细胞产品和食品添加剂，以防止体重增加。

74. 发明申请

WO2007145458A1 METHOD OF PREPARING siRNAs FOR SELECTIVE INHIBITION OF TARGET mRNA ISOTYPES 审中-公开
标题翻译：制备用于选择性抑制靶标mRNA同功酶的siRNA的方法
公开(公告)号：WO2007145458A1
公开(公告)日：2007-12-21
申请号：PCT/KR2007/002827
申请日：2007-06-12
申请人： BIONEER CORPORATION , PARK, Seong Min , KIM, Young Joo , CHOI, Young Chul , PARK, Han Oh , CHOUNG, So Rim
发明人： PARK, Seong Min , KIM, Young Joo , CHOI, Young Chul , PARK, Han Oh , CHOUNG, So Rim
IPC分类号： C12N15/10 , C12N15/11 , C12N15/00
CPC分类号： C12N15/111 , C12N2320/10
摘要： A method of preparing siRNAs for selective inhibition of target mRNA isotypes comprises: dividing target mRNA isotypes intended to inhibit the expression thereof and non-target mRNA isotypes from the mRNA isotypes of a gene; allotting a common location information region (A) of exons on genome DNA corresponding to the target mRNA isotypes; allotting a location information region (B) present specifically in exons of genome DNA corresponding to target mRNAs by excluding the location information region of exons on genome DNA corresponding to non-target mRNA from the location information region (A); determining base sequences in the target mRNAs corresponding to the location information region (B); and obtaining siRNA sequences for inhibiting the determined base sequences specifically. The method of the present invention can be used to prepare siRNAs for selective inhibition of specific target mRNA isotypes in a gene having several isotypes by alternative splicing, and enables siRNA design for all the genes in genome, making good tool for functional genomics study.
摘要翻译：制备用于选择性抑制靶mRNA同种型的siRNA的方法包括：从基因的mRNA同种型分离旨在抑制其表达的靶mRNA同种型和非靶mRNA同种型; 在对应于靶mRNA同种型的基因组DNA上分配外显子的共同位置信息区（A）; 通过从所述位置信息区域（A）中排除与非靶mRNA相对应的基因组DNA上的外显子的位置信息区域，分配特定存在于与靶mRNA相对应的基因组DNA外显子中的位置信息区（B）确定对应于位置信息区域（B）的目标mRNA中的碱基序列; 并获得特异性抑制所确定的碱基序列的siRNA序列。本发明的方法可用于制备用于通过选择性剪接在具有多个同种型的基因中特异性靶mRNA同种型的选择性抑制的siRNA，并且使得能够对基因组中所有基因进行siRNA设计，为功能基因组学研究提供了良好的工具。

75. 发明申请

WO2006062369A1 METHOD OF INHIBITING EXPRESSION OF TARGET MRNA USING SIRNA CONSISTING OF NUCLEOTIDE SEQUENCE COMPLEMENTARY TO SAID TARGET MRNA 审中-公开
标题翻译：抑制靶向MRNA的表达的方法使用SIRNA包含核酸序列与鉴定目标MRNA相符
公开(公告)号：WO2006062369A1
公开(公告)日：2006-06-15
申请号：PCT/KR2005/004207
申请日：2005-12-08
申请人： BIONEER CORPORATION , CHOI, Young-chul , PARK, Han Oh , CHOUNG, Sorim , KIM, Young Joo , KIM, Sang Soo , PARK, Seong-min , KIM, Sang-cheol , YOON, Gyuman , CHOI, Kyoung Oak , KANG, Hyo Jin
发明人： CHOI, Young-chul , PARK, Han Oh , CHOUNG, Sorim , KIM, Young Joo , KIM, Sang Soo , PARK, Seong-min , KIM, Sang-cheol , YOON, Gyuman , CHOI, Kyoung Oak , KANG, Hyo Jin
IPC分类号： C12Q1/68
CPC分类号： C12N15/111 , C12N15/113 , C12N2310/14 , C12N2320/11 , G06F19/18
摘要： A inhibition method of target mRNA expression includes: (a) obtaining binding energy of a double combination section on a dsRNA sequence of all combination comprising complementary nucleotides to a random target mRNA; (b) dividing the binding energy into four sections on the dsRNA sequence of each combination to obtain a difference of the mean binding energy between each section and convert into a score of a relative combination energy pattern; (c) selecting siRNA whose inhibition efficiency to target mRNA is expected to be high by applying the converted score to the dsRNA sequence with other factors that affect the efficiency of siRNA; and (d) inhibiting target mRNA expression using the selected siRNA. As a result, a researcher or an experimenter can analyze patterns of a relative binding energy on base sequences of unknown siRNA without actual experiments to determine whether the siRNA is effective or ineffective rapidly, thereby design and production efficiency of siRNA can be maximized and target mRNA can be effectively inhibited with efficient siRNA to the target mRNA.
摘要翻译：靶mRNA表达的抑制方法包括：（a）获得双组合切片对包含与随机靶mRNA的互补核苷酸的所有组合的dsRNA序列的结合能; （b）将结合能分成每个组合的dsRNA序列上的四个部分，以获得每个部分之间的平均结合能的差异，并转换成相对组合能量模式的得分; （c）通过将影响siRNA效率的其它因素应用于dsRNA序列的转化得分，选择siRNA靶向mRNA的抑制效率高的siRNA; 和（d）使用所选择的siRNA抑制靶mRNA表达。因此，研究人员或实验者可以分析未知siRNA碱基序列上的相对结合能力的模式，而无需实际的实验来快速确定siRNA是否有效或无效，从而可以使siRNA的设计和生产效率最大化，靶mRNA 可以有效地抑制靶mRNA的有效siRNA。

76. 发明申请

WO2006025703A1 MINIATURIZED APPARATUS FOR REAL-TIME MONITORING 审中-公开
标题翻译：用于实时监控的微型设备
公开(公告)号：WO2006025703A1
公开(公告)日：2006-03-09
申请号：PCT/KR2005/002899
申请日：2005-09-01
申请人： BIONEER CORPORATION , PARK, HAN Oh , PARK, Hanee , BAEK, Jong Soo
发明人： PARK, HAN Oh , PARK, Hanee , BAEK, Jong Soo
IPC分类号： C12Q1/68
CPC分类号： G01N21/645 , B01L3/5025 , B01L7/525 , B01L2300/0838 , B01L2300/0841 , B01L2300/087
摘要： The present invention relates to a apparatus for quantitative continuous real-time monitoring to monitor a continuous reaction of biochemical reagent and the reaction, such as DNA. More particularly, the present invention is directed to a miniaturized apparatus for real-time monitoring of biochemical reaction, which comprises capillary tubes (100) wherein biochemical reaction mixture flow; a thermal conduction block(120) which is coiled with capillary tube several rounds in order and composed of several blocks for temperature control of which temperatures are different from each other for heating or cooling the biochemical reaction mixture which flow in capillary tube, and a temperature controller which controls the temperature of above temperature control block; a radiation part(130) to radiate the reaction mixture flowing through the capillary tube and a light receiving section(140) which receives and measures the intensity of the flu¬ orescence generated from the capillary tubes.
摘要翻译：本发明涉及用于定量连续实时监测的装置，用于监测生物化学试剂和反应如DNA的连续反应。更具体地，本发明涉及一种用于实时监测生物化学反应的小型化装置，其包括其中生物化学反应混合物流动的毛细管（100）热传导块（120），其顺序地由毛细管螺旋成几圈，并且由用于温度彼此不同的温度控制的几个块组成，用于加热或冷却在毛细管中流动的生物化学反应混合物，温度控制器控制上述温度控制块的温度; 用于辐射流过毛细管的反应混合物的辐射部分（130）和接收并测量从毛细管产生的荧光强度的光接收部分（140）。

77. 发明申请

WO2006004267A1 DETECTION METHOD OF DNA AMPLIFICATION USING PROBE LABELED WITH INTERCALATING DYE 审中-公开
标题翻译：使用带有交联染料标记的探针进行DNA扩增的检测方法
公开(公告)号：WO2006004267A1
公开(公告)日：2006-01-12
申请号：PCT/KR2005/000889
申请日：2005-03-25
申请人： BIONEER CORPORATION , PARK, Han Oh , KIM, Hyun Bae , CHI, Sung Min
发明人： PARK, Han Oh , KIM, Hyun Bae , CHI, Sung Min
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/686 , C12Q2563/173 , C12Q2563/107 , C12Q2561/113
摘要： The present invention relates to a detection method of nucleic acid amplification using probe labeled with intercalating dye. More particularly, the present invention is directed to a real-time detection method of nucleic acid amplification, comprising the steps of i ) producing an aqueous buffer which contains a nucleic acid, a pair of primers for amplification of said nucleic acid, a fluorescent probe wherein a fluorescent dye of which intensity of fluorescence is varied when the dye is intercalated into a double-stranded nucleic acid, is connected with an oligonucleotide of which base sequence is complementary with at least a part of said nucleic acid, four(4) kinds of nucleotides and DNA polymerase; ii) denaturing said doublestranded nucleic acid into single strands by heating the aqueous buffer prepared in step i) up to 931C to 96C; iii) annealing said pair of primers with said single strand by cooling the solution obtained in step ii) up to 50 C to 571C; iv) replicating said single-stranded nucleic acid by heating the solution obtained from step iii) up to 701C to 74°C; v) denaturing said replicated nucleic acid into single strands by heating the solution obtained in step iv) up to 931C to 961C; vi) annealing said fluorescent probe with said single-stranded nucleic acid by cooling the solution obtained in step v up to 501C to 57 C ;vii) measuring an intensity of the fluorescence emitted from the solution obtained in step vi); and viii) repeating more than one steps iv) through vii).
摘要翻译：本发明涉及使用用插层染料标记的探针的核酸扩增检测方法。更具体地，本发明涉及核酸扩增的实时检测方法，包括以下步骤：i）产生含有核酸的水性缓冲液，用于扩增所述核酸的一对引物，荧光探针其中当染料插入双链核酸时荧光强度变化的荧光染料与其碱基序列与所述核酸的至少一部分互补的寡核苷酸连接，四（4）种的核苷酸和DNA聚合酶; ii）通过加热步骤i）中制备的含水缓冲液至931℃至96℃，将所述双链核酸变性为单链; iii）通过将步骤ii）中获得的溶液冷却至50℃至571℃，使所述单链引物退火; iv）通过将从步骤iii）获得的溶液加热至701℃至74℃来复制所述单链核酸; v）通过将步骤iv）中获得的溶液加热至931℃至961℃，将所述复制的核酸变性为单链; vi）通过将步骤v中获得的溶液冷却至501℃至57℃，用所述单链核酸退火所述荧光探针; vii）测量从步骤vi）中获得的溶液发射的荧光强度; 和viii）重复多于一个步骤iv）至vii）。

78. 发明申请

WO2004035831A1 METHOD FOR IDENTIFYING VEHICLE AND OLIGONUCLEOTIDE MARKER USED THEREFOR 审中-公开
标题翻译：识别其使用的车辆和寡核苷酸标记的方法
公开(公告)号：WO2004035831A1
公开(公告)日：2004-04-29
申请号：PCT/KR2003/002162
申请日：2003-10-16
申请人： BIONEER CORPORATION , GIL, Jun-mo , KIM, Young-hee , PARK, Han-oh , LEE, Sang-joo
发明人： GIL, Jun-mo , KIM, Young-hee , PARK, Han-oh , LEE, Sang-joo
IPC分类号： C12Q1/68
CPC分类号： C07H21/00 , C09D7/00 , C12Q1/68 , C12Q2563/185 , Y02P20/55
摘要： The present invention relates to a method for identifying vehicle and oligonucleotide marker used therefor. More particularly, the present invention is directed to a method for identifying vehicle by using oligonucleotide to which phase transfer agent is bound, and oligonucleotide marker used therefor.
摘要翻译：本发明涉及用于识别用于其的载体和寡核苷酸标记的方法。更具体地，本发明涉及通过使用结合相转移剂的寡核苷酸和用于其的寡核苷酸标记鉴定载体的方法。

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