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61. 发明申请

WO0040697A9 METHODS AND COMPOSITIONS FOR ISOLATION OF NUCLEIC ACID MOLECULES 审中-公开
标题翻译：分离核酸分子的方法和组合物
公开(公告)号：WO0040697A9
公开(公告)日：2001-08-02
申请号：PCT/US0000170
申请日：2000-01-06
申请人： LIFE TECHNOLOGIES INC
发明人： BLAKESLEY ROBERT W
IPC分类号： C12N15/09 , C07H1/06 , C12N15/10 , C12P19/34 , C12N1/08 , C07H15/12
CPC分类号： C07H1/06 , C12N15/101
摘要： The present invention relates generally to compositions, methods and kits for use in isolating nucleic acid molecules. More specifically, the invention relates to such compositions, methods and kits that are useful in the isolation of nucleic acid molecules from cells (e.g., bacterial cells, animal cells, fungal cells, viruses, yeast cells or plant cells) via lysis and one or more additional isolation steps, such as one or more chromatography steps. In particular, the invention relates to compositions, methods and kits wherein nucleic acid molecules are isolated using an integrated lysis/chromatography matrix, which may comprise one or more supports (e.g., polyolefin, scintered polyethylene, nitrocellulose, polypropylene, polycarbonate, cellulose acetate, silica, and the like) that has been treated with or associated with one or more chemical lysis reagents (e.g., one or more detergents, one or more chaeotropes, one or more enzymes, and the like). The compositions, methods and kits of the invention are suitable for isolating a variety of forms of nucleic acid molecules from cells, including but not limited to plasmids, vectors, DNA, cDNA, RNA, mitochondrial DNA, chloroplast DNA, and the like, any of which may be single-stranded, double-stranded, linear or circular. The compositions, methods and kits of the invention are particularly well-suited for rapid isolation of soluble plasmid or vector DNA from bacterial cells.
摘要翻译：本发明一般涉及用于分离核酸分子的组合物，方法和试剂盒。更具体地，本发明涉及可用于通过裂解从细胞（例如细菌细胞，动物细胞，真菌细胞，病毒，酵母细胞或植物细胞）分离核酸分子的这样的组合物，方法和试剂盒，更多额外的分离步骤，例如一个或多个色谱步骤。特别地，本发明涉及组合物，方法和试剂盒，其中使用整合的裂解/色谱基质分离核酸分子，其可以包含一种或多种载体（例如聚烯烃，烧结聚乙烯，硝化纤维素，聚丙烯，聚碳酸酯，乙酸纤维素，二氧化硅等），其已被一种或多种化学裂解试剂（例如，一种或多种洗涤剂，一种或多种螯合剂，一种或多种酶等）处理或与之相关联。本发明的组合物，方法和试剂盒适用于从细胞分离多种形式的核酸分子，包括但不限于质粒，载体，DNA，cDNA，RNA，线粒体DNA，叶绿体DNA等，任何其可以是单链，双链，线性或圆形。本发明的组合物，方法和试剂盒特别适用于从细菌细胞中快速分离可溶性质粒或载体DNA。

62. 发明申请

WO99024590A1 SUICIDE EXPRESSION VECTOR FOR VACCINE STRAINS 审中-公开
标题翻译：疫苗菌株SUICIDE EXPRESSION VECTOR FOR VACCINE STRAINS
公开(公告)号：WO99024590A1
公开(公告)日：1999-05-20
申请号：PCT/AU1998/000930
申请日：1998-11-06
IPC分类号： C12N1/08 , C12N9/22 , C12N15/63 , C12N15/74
CPC分类号： C12N15/74 , C12N1/08 , C12N9/22 , C12N15/63
摘要： A suicide expression vector for expressing a heterologous peptide, polypeptide or protein in a selected host cell, said vector comprising: (i) a first nucleotide sequence encoding said heterologous peptide, polypeptide or protein (e.g. sperm antigens, esterases capable of hydrolysing organophosphates and insecticidal toxins) operably linked to a first promoter sequence; (ii) a second nucleotide sequence encoding a restriction enzyme or functional portion thereof operably linked to a second promoter sequence, said second promoter sequence being inducible; and (iii) one or more cleavage site(s) for said restriction enzyme or functional portion thereof, said cleavage site(s) being absent from the chromosomal DNA of said host cell, wherein upon introduction of the vector into said host cell, induced expression of the restriction enzyme or functional portion thereof from said second nucleotide sequence brings about the cleavage of the suicide expression vector. The vector is useful for the production of microorganisms intended for environmental release in the applications of, for example, vaccine baits for sterilisation of vermin, compositions for bioremediation of contaminated soils and waste, and insecticidal compositions for crop spraying.
摘要翻译：一种用于在所选宿主细胞中表达异源肽，多肽或蛋白质的自杀表达载体，所述载体包含：（i）编码所述异源肽，多肽或蛋白质的第一核苷酸序列（例如精子抗原，能够水解有机磷酸酯和杀虫剂的酯酶毒素）可操作地连接到第一启动子序列; （ii）编码与第二启动子序列可操作地连接的限制酶或功能部分的第二核苷酸序列，所述第二启动子序列是可诱导的; 和（iii）用于所述限制酶或其功能部分的一个或多个切割位点，所述切割位点不存在于所述宿主细胞的染色体DNA中，其中当将所述载体导入所述宿主细胞时，诱导来自所述第二核苷酸序列的限制酶或其功能部分的表达导致自杀表达载体的切割。该载体可用于生产用于环境释放的微生物，例如用于灭虫害虫的疫苗诱饵，用于生物修复污染的土壤和废物的组合物，以及用于作物喷洒的杀虫组合物。

63. 发明申请

WO9322332A3 RECOMBINANT PRODUCTION OF IMMUNOGLOBULIN-LIKE DOMAINS IN PROKARYOTIC CELLS 审中-公开
标题翻译：在原核细胞中重组生物素免疫球蛋白
公开(公告)号：WO9322332A3
公开(公告)日：1994-02-17
申请号：PCT/US9303895
申请日：1993-04-26
申请人： UNIV TEXAS
发明人： WARD ELIZABETH SALLY , KIM JIN-KYOO
IPC分类号： A61K47/48 , C07K14/705 , C07K14/725 , C07K14/73 , C07K14/735 , C07K14/74 , C07K16/00 , C12N1/21 , C12P21/04 , C07H23/00 , C12N1/00 , C12N1/08 , C12N1/20 , C12N15/00 , C12P19/34 , C12Q1/68
CPC分类号： C07K16/00 , A61K47/6827 , A61K47/6849 , C07K14/70503 , C07K14/7051 , C07K14/70514 , C07K14/70535 , C07K14/70539 , C07K2317/52 , C07K2317/53 , C07K2319/00
摘要： Disclosed are recombinant vectors encoding immunoglobulin-like domains and portions thereof, such as T-cell variable domains, antidoby Fc-hinge fragments, subfragments and mutant domains with reduced biological half lives. Methods of producing large quantities of such domains, heterodimers, and fusion proteins following expression and secretion by prokaryotic host cells are also reported. Described are single chain T-cell receptors, which are folded into β-pleated sheet structures similar to those of immunoglobulin variable domains; antibody Fc and Fc-hinge domains, which have the same in vivo stability as intact antibodies; and domains engineered to have reduced in vivo half lives. These DNA constructs and protein domains will be useful as templates for in vitro mutagenesis and high resolution structural studies; for immunization and vaccination; and for the production of recombinant antibodies or chimaeric proteins with increased or decreased stability and longevity for therapeutic and diagnostic uses.
摘要翻译：公开了编码免疫球蛋白样结构域及其部分的重组载体，例如具有降低的生物半衰期的T细胞可变结构域，抗体Fc-铰链片段，亚片段和突变结构域。还报道了在原核宿主细胞表达和分泌后产生大量此类结构域，异源二聚体和融合蛋白的方法。描述的是单链T细胞受体，其被折叠成类似于免疫球蛋白可变结构域的β-折叠片结构; 抗体Fc和Fc-铰链结构域，其具有与完整抗体相同的体内稳定性; 并设计了减少半衰期的领域。

64. 发明申请

WO2021257803A1 ADAS COMPRISING BACTERIAL SECRETION SYSTEMS 审中-公开
公开(公告)号：WO2021257803A1
公开(公告)日：2021-12-23
申请号：PCT/US2021/037787
申请日：2021-06-17
申请人： FLAGSHIP PIONEERING INNOVATIONS VI, LLC
发明人： TAM, Hok Hei , KOLESKY, David Barry , REEVES, Analise Zaunbrecher , SPAULDING, Caitlin Nicole , HUBBARD, Troy Patrick , FISHER, Adam Barclay , HOMAN, Kimberly A.
IPC分类号： A61K35/66 , C07K14/195 , C12N1/08 , C12N15/74
摘要： The invention provides isolated achromosomal dynamic active systems (ADAS), including ADAS comprising secretion systems. These ADAS provided by the invention can be obtained by a variety of means. Various associated methods of making and using these ADAS are provided.

65. 发明申请

WO2021172217A1 核酸の低減方法および吸着フィルター审中-公开
公开(公告)号：WO2021172217A1
公开(公告)日：2021-09-02
申请号：PCT/JP2021/006411
申请日：2021-02-19
申请人：株式会社カネカ
发明人：鴻池　史憲 , 水口　和信 , 村田　大 , 岸　鉄馬 , 西八條　正克
IPC分类号： C07K1/22 , C07K1/30 , C07K16/00 , C12N7/02 , B01D15/38 , B01J20/04 , C12N1/08
摘要：本発明は、液体における特定の不純物の量を有効かつ簡便に低減することができる方法を提供することを目的とする。本発明に係る液体中の核酸の量を低減する方法は、前記液体を水不溶性マグネシウム化合物に接触させることにより、前記核酸の少なくとも一部を前記水不溶性マグネシウム化合物に吸着させる工程を含むことを特徴とする。また、本発明は、ＤＮＡ等に対する吸着能に優れる一方で、抗体や抗体様分子に対する吸着性は低いことから、目的物の収率を簡便に高位に維持することができ、不純物を効率的に除去可能な精製用資材として、抗体および抗体様分子などの有用物質の精製に有用な吸着フィルターを提供することを目的とする。本発明に係る吸着フィルターは、水不溶性マグネシウム化合物を含む層を有することを特徴とする。

66. 发明申请

WO2020214124A1 USE OF ANTI-HISTONE ANTIBODIES FOR REMOVING EUCARYOTIC DNA DURING ISOLATION OF BACTERIA DNA AND CHROMATIN IMMUNO- PRECIPITATION METHOD 审中-公开
公开(公告)号：WO2020214124A1
公开(公告)日：2020-10-22
申请号：PCT/TR2020/050321
申请日：2020-04-15
申请人： ISTANBUL MEDIPOL UNIVERSITESI
发明人： DEMIRCAN, Turan , SENGIL, Ahmet Zeki
IPC分类号： C12N1/08 , C12N15/10
摘要： The present invention is related to a kit suitable to be used for complete genome sequencing in metagenomic-microbiome studies and a genome sequencing method where said kit is used.

67. 发明申请

WO2020182970A1 PROCESS FOR MAKING STABLE BACTERIAL EXTRACTS AND THEIR USE AS PHARMACEUTICALS 审中-公开
公开(公告)号：WO2020182970A1
公开(公告)日：2020-09-17
申请号：PCT/EP2020/056742
申请日：2020-03-12
申请人： OM PHARMA SA
发明人： BAUER, Jacques , CHRISTIAN, Pasquali
IPC分类号： A61K35/74 , A61K9/00 , A61K39/02 , A61M15/08 , C12N1/06 , C12N1/08 , C12N1/20 , A61P37/00 , A61P31/00
摘要： The present invention relates to novel stable bacterial extract preparations having substantial increased stability overtime, novel methods of preparation thereof, pharmaceutical formulations based on these novel stabilized bacterial extracts, as well as novel routes of administration and delivery devices for treating and/or preventing acute and chronic immunological disorders resulting from infections and/or inflammation and/or neoplasms and/or dysbiosis.

68. 发明申请

WO2020131952A1 COMPOSITIONS AND METHODS FOR REMOVING DENTAL CALCULI 审中-公开
公开(公告)号：WO2020131952A1
公开(公告)日：2020-06-25
申请号：PCT/US2019/066969
申请日：2019-12-17
申请人： MYNARCIK, Dennis C.
发明人： MYNARCIK, Dennis C.
IPC分类号： C12N1/08 , C12N9/14 , C12N9/16 , C12P19/34 , C11D3/386 , D06M101/10 , C08L3/06
摘要： Disclosed are compositions and formulations comprising enzymes or other biocatalyst that cleave surface-accessible DNA polymers and/or glycoprotein carbohydrate chains at galactose residues in dental calculus, and optionally further include one or more proteolytic enzymes, thereby destroying the structural integrity of the calculus, and allowing it to be readily removed without requiring special treatment by a trained dental professional. Also disclosed are methods for removing dental calculus using the disclosed compositions and formulations.

69. 发明申请

WO2018210794A1 ENZYME PRODUCTS 审中-公开
公开(公告)号：WO2018210794A1
公开(公告)日：2018-11-22
申请号：PCT/EP2018/062476
申请日：2018-05-15
申请人： C-LECTA GMBH
发明人： SCHÖNERT, Stefan , SALOMO, Mathias , SCHULTCHEN, Thomas , VOGEL, Andreas , KÖPKE, Sabrina , BARTSCH, Sebastian , BRUCHER, Birgit , FELLER, Claudia
IPC分类号： C12N9/00 , C07K1/14 , C12N1/08 , C12N9/10 , C12N9/22 , C07K1/34
摘要： The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.

70. 发明申请

WO2018115042A1 REMOVAL OF NUCLEIC ACIDS AND FRAGMENTS THEREOF FROM A BIOMASS MATERIAL 审中-公开
公开(公告)号：WO2018115042A1
公开(公告)日：2018-06-28
申请号：PCT/EP2017/083683
申请日：2017-12-20
申请人： UNIBIO A/S
发明人： NTOKOU, Eleni , NANDY, Subir, Kumar
IPC分类号： A23J3/20 , C12N1/08 , A23K10/16 , A23J3/14 , B01D15/26 , B01D61/14 , A23L33/195
摘要： The present invention relates to a method for providing a SCP product from a biomass material wherein said SCP product comprising a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids in the biomass material, the method comprising the steps of: (i) providing the biomass material; (ii) subjecting the biomass material to a process of cell disruption, providing a disrupted biomass material; (iii) applying the disrupted biomass material to a first separation process resulting in a first retentate comprising proteins and/or cell debris, and a first permeate comprising nucleic acids; (iv) subjecting the first permeate to a second treatment separating the nucleic acids from vitamins, minerals and/or amino acids; (v) optionally, combining the first retentate obtained in step (iii) with the vitamins, minerals and/or amino acids obtained in step (iv), providing the SCP product comprising a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids.

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