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61. 发明申请

WO98037212A1 IMPROVED TRANSFORMATION METHOD FOR PLANTS 审中-公开
标题翻译：改进植物转化方法
公开(公告)号：WO98037212A1
公开(公告)日：1998-08-27
申请号：PCT/IB1998/000220
申请日：1998-02-20
IPC分类号： C12N15/09 , A01H1/00 , C12N5/04 , C12N5/10 , C12N15/29 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8201 , C12N15/8205
摘要： A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of: 1) incubating, prior to contacting with the DNA fragment, a culture of untransformed monocotyledonous plant cells on a medium comprising a plant phenolic compound, for a period of time sufficient to stimulate cell division and enhance competence for integration of foreign DNA; and 2) contacting the untransformed cells with the DNA fragment under conditions in which the DNA fragment is taken up by the untransformed cells and is stably integrated in the genome of the untransformed cells, to generate transformed cells.
摘要翻译：将DNA片段整合到单子叶植物细胞的基因组中的方法，该方法包括以下步骤：1）在与DNA片段接触之前，在包含植物的培养基上孵育未转化的单子叶植物细胞的培养物酚类化合物，足以刺激细胞分裂并增强外源DNA整合能力的时间; 和2）在DNA片段被未转化细胞吸收的条件下将未转化的细胞与DNA片段接触并稳定地整合到未转化的细胞的基因组中，以产生转化的细胞。

62. 发明申请

WO1997042334A1 VECTOR FOR GENE TRANSFER INTO PLANT ALLOWING OPTIONAL DELETION OF MARKER GENE 审中-公开
标题翻译：遗传转基因植物允许标记基因的删除
公开(公告)号：WO1997042334A1
公开(公告)日：1997-11-13
申请号：PCT/JP1997001569
申请日：1997-05-09
申请人： NIPPON PAPER INDUSTRIES CO., LTD. , SUGITA, Koichi , UESUGI, Mikiko , MATSUNAGA, Etsuko , EBINUMA, Hiroyasu
发明人： NIPPON PAPER INDUSTRIES CO., LTD.
IPC分类号： C12N15/84
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8209 , C12N15/8213 , C12N15/8242
摘要： A vector for gene transfer into a plant allowing the deletion of a marker gene transferred into the plant together with the target gene, if necessary after the expression thereof, from the DNA of the chromosome, etc. wherein the marker gene exists and acts to thereby eliminate its function and to detect the expression or elimination of the function of the marker gene on the basis of morphological changes in the tissues originating in the plant cells into which the genes have been transferred. The vector is constructed by using a gene capable of inducing morphological abnormality as the marker gene and controlling a DNA factor capable of leaving with a regulator so that the morphological abnormality-inducing gene is located at such a position as to act together with the DNA factor but the target gene is not located at such a position as to act together therewith.
摘要翻译：用于基因转移到植物中的载体，其允许从染色体的DNA等到目的基因的转移到植物中的标记基因（如果必要的话）从染色体的DNA等中删除，其中标记基因存在并作用于其消除其功能，并根据基因转移到植物细胞中的组织的形态变化来检测标记基因的功能的表达或消除。通过使用能够诱导形态异常的基因作为标记基因构建载体，并通过调节剂控制能够离去的DNA因子，使得形态异常诱导基因位于与DNA因子一起作用的位置但靶基因并不位于与其一起作用的位置。

63. 发明申请

WO1997013401A1 METHOD FOR THE PRODUCTION OF HYBRID PLANTS 审中-公开
标题翻译：混合植物生产方法
公开(公告)号：WO1997013401A1
公开(公告)日：1997-04-17
申请号：PCT/US1996016418
申请日：1996-10-11
申请人： PURDUE RESEARCH FOUNDATION , HODGES, Thomas, K. , LYZNIK, Leszek, A. , VINCENT, Jeffrey , KONONOWICZ-HODGES, Halina , HUQ, Enamul , LEE, Jang-Yong
发明人： PURDUE RESEARCH FOUNDATION
IPC分类号： A01H01/02
CPC分类号： C12N15/8216 , A01H1/02 , C07K14/415 , C12N15/8201 , C12N15/8217 , C12N15/8237 , C12N15/8239 , C12N15/8261 , C12N15/8282 , C12N15/8289 , Y02A40/146
摘要： The present invention is directed to recombinant expression vectors comprising a suicide gene flanked by site-specific recombination sequences and the use of those expression vectors to produce transgenic plants that facilitate the production of hybrid plants. The transgenic plants produced in accordance with the present invention comprise a novel male-sterility/restorer system, wherein a male sterile plant is cross-fertilized with a restorer plant to produce a hybrid plant.
摘要翻译：本发明涉及包含侧翼为位点特异性重组序列的自杀基因的重组表达载体以及这些表达载体用于产生促进杂交植物生产的转基因植物的用途。根据本发明生产的转基因植物包含一种新的雄性不育/恢复系统，其中雄性不育植物与恢复植物交叉受精以产生杂种植物。

64. 发明申请

WO1994010329A1 RNA PACKAGING SYSTEM 审中-公开
标题翻译： RNA包装系统
公开(公告)号：WO1994010329A1
公开(公告)日：1994-05-11
申请号：PCT/US1993010396
申请日：1993-10-28
申请人： RUTGERS UNIVERSITY
发明人： RUTGERS UNIVERSITY , WILSON, Thomas, M., A. , HWANG-LEE, Duk-Ju
IPC分类号： C12P19/34
CPC分类号： C07K14/005 , C12N7/00 , C12N15/10 , C12N15/8201 , C12N15/8206 , C12N2770/00022 , Y10S435/81
摘要： The present invention relates to an in vivo system for expression and packaging of recombinant RNA into pseudovirus particles. The invention is based on the discovery that plant viral coat proteins (CPs) may be efficiently expressed in E. coli, and that these recombinant coat proteins will function to assemble in vivo and package recombinant chimeric RNA, containing an operatively linked origin-of-assembly (OAS) sequence, to form mature viral particles containing a foreign RNA. The present invention thus provides for packaging of RNA into a ribonuclease-resistant form that is easily purified and stored, and which overcomes the prior art problems associated with degradation of RNA by ribonucleases. Significantly, the method of the invention is RNA sequence- and length-independent. The components of the invention include a source in the bacterial host of viral coat proteins, and a source in the bacterial host to direct the transcription of a DNA molecule comprising an OAS-encoding DNA and a foreign DNA, which DNA molecule can be transcribed in the host cell to produce an RNA molecule comprising an OAS operatively linked to an RNA of interest. The CPs and OAS are from a plant virus having a rod-shaped helical particle and a single-stranded RNA genome, most preferably tobacco mosaic virus.
摘要翻译：本发明涉及用于将重组RNA表达和包装成假病毒颗粒的体内系统。本发明基于以下发现：可以在大肠杆菌中有效表达植物病毒外壳蛋白（CP），并且这些重组外壳蛋白将用于在体内组装并包装重组嵌合RNA，所述重组嵌合RNA含有可操作地连接的起始蛋白组装（OAS）序列，以形成含有外源RNA的成熟病毒颗粒。因此，本发明提供将RNA包装到易于纯化和储存的核糖核酸酶抗性形式中，并克服了与核糖核酸酶降解RNA相关的现有技术问题。显着地，本发明的方法是RNA序列和长度无关的。本发明的组分包括病毒外壳蛋白的细菌宿主中的源，以及细菌宿主中用于引导包含编码OAS的DNA和外来DNA的DNA分子的转录的来源，该DNA分子可被转录在宿主细胞以产生包含可操作地连接到目的RNA的OAS的RNA分子。 CP和OAS来自具有棒状螺旋颗粒和单链RNA基因组的植物病毒，最优选烟草花叶病毒。

65. 发明申请

WO2016100624A1 COMPOSITIONS AND METHODS FOR IMPROVING ABIOTIC STRESS TOLERANCE 审中-公开
标题翻译：用于改善生物耐压性的组合物和方法
公开(公告)号：WO2016100624A1
公开(公告)日：2016-06-23
申请号：PCT/US2015/066298
申请日：2015-12-17
申请人： SYNGENTA PARTICIPATIONS AG , SYNGENTA CROP PROTECTION, LLC
发明人： BASU, Shib Sankar
IPC分类号： C12N5/14 , C07K14/415
CPC分类号： C12N15/8261 , A01H1/02 , C07K14/415 , C12N15/8201 , C12N15/8271 , C12N15/8273 , C12Q1/6895 , Y02A40/146
摘要： The present invention relates to compositions and methods for improving yield, yield stability, and/or drought stress tolerance in plants. Plants and/or plant parts identified, selected and/or produced using compositions and methods of the present invention are also provided.
摘要翻译：本发明涉及用于在植物中提高产量，产量稳定性和/或干旱胁迫耐受性的组合物和方法。还提供了使用本发明的组合物和方法鉴定，选择和/或生产的植物和/或植物部分。

66. 发明申请

WO2016094029A1 METHODS OF EMBRYOGENIC TISSUE PREPARATION FOR SUGAR CANE TRANSFORMATION 审中-公开
标题翻译：用于蔗糖转化的胚芽组织制备方法
公开(公告)号：WO2016094029A1
公开(公告)日：2016-06-16
申请号：PCT/US2015/060535
申请日：2015-11-13
申请人： SYNGENTA PARTICIPATIONS AG , SYNGENTA CROP PROTECTION LLC
发明人： MAI, Pearl , DUNDER, Erik
IPC分类号： C12N15/82
CPC分类号： C12N5/04 , C12N15/8201 , C12N15/8205
摘要： Methods of tissue preparation for the transformation of sugar cane are provided. The methods comprise a pre-culture treatment of sugar cane embryogenic tissue prior to transformation. The methods comprise excising a segment of plant tissue from a shoot of sugar cane; culturing said segment to produce sugar cane embryogenic tissues; performing a pre-culturing treatment by sub-culturing responding embryogenic tissue on fresh media for a period of time of at least 7 days, in the same media; and with no intervening step and no changes of media, performing transformation of embryogenic tissue. Transformation can be performed via Agrobacterium-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.
摘要翻译：提供了甘蔗转化的组织制备方法。所述方法包括在转化之前对甘蔗胚发生组织的预培养处理。该方法包括从甘蔗芽切除一段植物组织; 培养所述片段以产生甘蔗胚发生组织; 通过在相同的培养基中将新鲜培养基上的反应性胚发生组织分别培养至少7天的时间进行预培养处理; 并没有介入步骤，也没有介质的变化，进行胚胎发生组织的转化。转化可以通过农杆菌介导的基因递送，生物弹性转化等进行。转基因植物从有利于转化细胞生长的条件下生长的小植株再生，同时基本上抑制未转化细胞的生长。

67. 发明申请

WO2016009006A1 TOBACCO PROTEASE GENES 审中-公开
标题翻译：烟草蛋白酶基因
公开(公告)号：WO2016009006A1
公开(公告)日：2016-01-21
申请号：PCT/EP2015/066341
申请日：2015-07-16
申请人： PHILIP MORRIS PRODUCTS S.A
发明人： BOVET, Lucien , FLORACK, Dion , BATTEY, James
IPC分类号： A01H5/12 , C12N9/50 , C12N15/82
CPC分类号： A24B3/12 , A01H5/12 , C12N9/1029 , C12N9/104 , C12N9/48 , C12N9/63 , C12N15/8201 , C12N15/8213 , C12N15/8242 , C12N15/8261 , C12Y203/01091 , C12Y203/02002 , C12Y304/21107 , C12Y304/21112 , C12Y304/22 , C12Y304/22002 , C12Y304/23 , C12Y304/24 , Y02A40/146
摘要： The invention provides protease genes which are regulated in a specific manner during curing of tobacco plants material and which affect the flavour of cured tobacco.
摘要翻译：本发明提供了在烟草植物材料固化期间以特定方式调节并影响固化烟草的风味的蛋白酶基因。

68. 发明申请

WO2015171573A1 SYSTEM FOR SEED PREPARATION AND METHOD OF USE 审中-公开
标题翻译：种子制备系统及其使用方法
公开(公告)号：WO2015171573A1
公开(公告)日：2015-11-12
申请号：PCT/US2015/029185
申请日：2015-05-05
申请人： DOW AGROSCIENCES LLC
发明人： MCCARTY II, Donald L. , CHENNAREDDY, Sivarama R. , CICAK, Toby , SARRIA, Rodrigo , GILLESPIE, David T. , PARSONS, Thomas J. , KALEYTA, Scott R.
IPC分类号： A01C1/00 , A01G7/00
CPC分类号： A01C1/00 , B25J9/1697 , B25J15/0683 , B25J17/0225 , B26D1/04 , B26D3/08 , B26D3/30 , B26D5/00 , B26D5/007 , B26D7/02 , B26D7/06 , B64G1/00 , C12N15/8201 , G01N1/286 , G01N35/0099 , G01N2001/2873 , Y10T83/536 , Y10T83/6572
摘要： A system and method for the automated or semi-automated preparation of seeds for transformation and transgenic engineering.
摘要翻译：用于转化和转基因工程的种子的自动或半自动化准备的系统和方法。

69. 发明申请

WO2015158919A1 MAIZE PLASTID TRANSFORMATION METHOD 审中-公开
标题翻译： MAIZE PLASTID转换方法
公开(公告)号：WO2015158919A1
公开(公告)日：2015-10-22
申请号：PCT/EP2015/058449
申请日：2015-04-17
申请人： MØLLER, Simon Geir , DUNNE, Aisling , CHUA, Nam-Hai
发明人： MØLLER, Simon Geir , DUNNE, Aisling , CHUA, Nam-Hai
IPC分类号： C12N15/82
CPC分类号： C12N15/821 , A01H4/008 , C12N15/8201 , C12N15/8207 , C12N15/8213 , C12N15/8214 , C12N15/8294
摘要： The present invention relates to processes for the transformation of plant tissues with a genetic construct which comprises a transgene and a selection gene. The selection gene preferably encodes an auxin biosynthetic polypeptide, thus allowing for selection of transformed plants on media lacking plant auxins. The invention particularly relates to processes wherein the selection step is carried out under a light/dark cycle.
摘要翻译：本发明涉及用包含转基因和选择基因的遗传构建体转化植物组织的方法。选择基因优选编码生长素生物合成多肽，从而允许在缺乏植物生长素的培养基上选择转化的植物。本发明特别涉及其中选择步骤在光/暗循环下进行的方法。

70. 发明申请

WO2015103070A1 TRAIT STACKING STRATEGY FOR CORN INTROGRESSION 审中-公开
标题翻译： CORN INTROGRESSION的TRAIT堆栈策略
公开(公告)号：WO2015103070A1
公开(公告)日：2015-07-09
申请号：PCT/US2014/072333
申请日：2014-12-24
申请人： DOW AGROSCIENCES LLC
发明人： MEYER, Jenelle , BING, James, W.
IPC分类号： A01H5/00
CPC分类号： C12N15/8286 , A01H1/02 , A01H1/04 , C12N15/8201 , C12N15/8241 , C12N15/8275 , C12N15/8277 , C12Q1/6895 , Y02A40/162
摘要： A method is provided to decrease the time required to introgress three or more desired traits from donor plant lines into an elite plant background. The method comprises crossing two donor plants, wherein the donor plants have each been backcrossed to have a high recurrent parent percentage and share one desired locus to be introgressed into the elite plant background.
摘要翻译：提供了一种方法来减少从供体植物系进入精英植物背景的三种或更多种所需特征所需的时间。该方法包括交叉两个供体植物，其中供体植物各自被回交以具有高的轮回亲本百分比并且共享要渗入精英植物背景的一个所需要的基因座。

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