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    • 63. 发明申请
    • METHOD OF SELECTING AT LEAST ONE MUTATION SCREEN, ITS APPLICATION TO A METHOD FOR RAPID IDENTIFICATION OF ALLELES OF POLYMORPHOUS SYSTEMS AND DEVICE FOR IMPLEMENTATION THEREOF
    • 选择至少一个突变屏幕的方法,其应用于用于快速识别多态性系统的方法的方法及其实现的装置
    • WO1993011262A1
    • 1993-06-10
    • PCT/FR1992001141
    • 1992-12-03
    • BERTIN & CIECOHEN, NadineBOUGUELERET, LydieCOHEN, DanielDAUSSET, Jean
    • BERTIN & CIE
    • C12Q01/68
    • C12Q1/6827C12Q1/6881C12Q2600/156
    • Methods of selecting at least one mutation screen from a series of allele sequences of a polymorphous gene and for rapid identification of alleles of polymorphous genes, nucleotide probes obtained from the said mutation screens, placed specifically in data bank form, and a device for implementing the said method are disclosed. The method for identifying alleles consists of: (a) selecting all or part of a known consensus sequence of the said polymorphous gene; (b) creating a mutation matrix for the corresponding sequences of known alleles; (c) identifying indiscernable sequences by comparison in twos (alleles having the same mutation profile in the sequence selected in (a)) and excluding one of the members of the said pairs; (d) identifying and counting the obligatory mutations or allele marker mutations, i.e. those which are necessary and adequate for distinguishing between two alleles which are otherwise identical (set O of obligatory mutations); and (f) obtaining the said minimum mutation screen(s), comprising at least the obligatory mutations of step (e); then (g) selecting from the screen selected in step (f) of the said mutation screen selection process the most suitable mutation screen for preparing oligonucleotide probes suitable for use in differenciating all the alleles; (h) appropriately hybridizing an allele X to be identified with the oligonucleotide probes selected from the mutation screen(s) obtained in steps (a) to (g); and (i) identifying the allele X by detection of the said hybrid(s) which may have been formed in step (h).
    • 从多晶型基因的一系列等位基因序列中选择至少一个突变筛选的方法以及用于快速鉴定多形性基因等位基因,从所述突变筛选获得的核苷酸探针,特别放置在数据库形式中的装置,以及用于实施 所述方法被公开。 用于鉴定等位基因的方法包括:(a)选择所述多态基因的已知共有序列的全部或部分; (b)为已知等位基因的相应序列产生突变基因; (c)通过比较确定不确定序列(在(a)中选择的序列中具有相同突变特征的等位基因)并排除所述对中的一个成员; (d)识别和计数强制性突变或等位基因标记突变,即那些必须和足以区分两种相同的(等同性突变的O)等位基因之间的突变; 和(f)获得所述最小突变筛选,至少包含步骤(e)的必需突变; 然后(g)从所述突变筛选选择过程的步骤(f)中选择的筛选中选择适合用于区分所有等位基因的最适合的用于制备寡核苷酸探针的突变筛选; (h)将待鉴定的等位基因X与选自步骤(a)至(g)中获得的突变筛选的寡核苷酸探针适当杂交; 和(i)通过检测可能已经在步骤(h)中形成的所述杂交体鉴定等位基因X.
    • 69. 发明申请
    • PROCESS AND DEVICE FOR CONTROLLED MIGRATION OF MACROMOLECULES IN RECTANGULAR GEL PLATES BY MULTIPLE ELECTROPHORESIS
    • 通过多种电泳法控制矩形凝胶板中大分子的迁移的方法和装置
    • WO1990002601A1
    • 1990-03-22
    • PCT/FR1989000441
    • 1989-09-04
    • BERTIN & CIEDUTERTRE, Bernard
    • BERTIN & CIE
    • B01D57/02
    • B01D57/02C07K1/26G01N27/4473G01N27/44773
    • Process and device for controlled migration, by multiple electrophoresis, of macromolecules in gel plates stacked perpendicular to their plane, using a series of electrodes (20) arranged at the intersections of two series of perpendicular planes, some perpendicular to the plates and to the desired direction of migration of the macromolecules, the others perpendicular to the former and parallel to the plates (12). The electrodes are interconnected in a selective and controlled manner so as to ensure, on the one hand, the separation of the marcomolecules in the plates (12) and, on the other hand, their transfer to the membranes (22) associated with the plates (12). The invention is applicable in particular to the separation and transfer by electrophoresis of macromolecules such as proteins and nucleic acids.
    • 使用一系列布置在两系列垂直平面的交点处的一系列电极(20),通过多次电泳控制迁移的大分子在垂直于它们的平面堆叠的凝胶板中的过程和装置,一些垂直于板的交点和期望的 大分子的迁移方向,其他垂直于前者并平行于板(12)的方向。 电极以选择性和受控的方式相互连接,以便一方面确保板(12)中的分子分离,另一方面确保它们转移到与板相关的膜(22) (12)。 本发明特别适用于通过电泳分离和转移大分子如蛋白质和核酸。