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    • 65. 发明申请
    • ENGINEERED MICROORGANISMS FOR PRODUCING ISOPROPANOL
    • 用于生产异丙酚的工程微生物
    • WO2008131286A1
    • 2008-10-30
    • PCT/US2008/060911
    • 2008-04-18
    • GEVO, INC.SUBBIAN, EzhilkaniMEINHOLD, PeterBUELTER, ThomasHAWKINS, Andrew C.
    • SUBBIAN, EzhilkaniMEINHOLD, PeterBUELTER, ThomasHAWKINS, Andrew C.
    • C12P7/66C12N1/20
    • C12P7/04
    • In an embodiment, there is disclosed a recombinant microbial host cell having each of the DNA molecules encoding a polypeptide or group of polypeptides that catalyze the conversion: (i) Acetyl-CoA to Acetate and CoA (conversion 1) (ii) Acetyl-CoA to Acetoacetyl-CoA and CoA (conversion 2) (iii) Acetoacetyl-CoA and Acetate to Acetoacetate and Acetyl-CoA (conversion 3.1) (iv) Acetoacetate to Acetone and CO2 (conversion 4) (v) Acetone and NAD(P)H and H+ to Isopropanol and NAD(P)+ (conversion 5) wherein the at least one DNA molecule is heterologous to the microbial host cell and wherein the microbial host cell produces isopropanol. In another embodiment, a method is disclosed for the production of isopropanol including providing a recombinant microbial host cell, the host cell of (i) with a fermentable carbon substrate in a fermentation medium under conditions whereby isopropanol is produced, and recovering the isopropanol.
    • 在一个实施方案中,公开了一种重组微生物宿主细胞,其具有编码催化转化的多肽或多肽组的每个DNA分子:(i)乙酰辅酶A至乙酸酯和CoA(转化1)(ii)乙酰辅酶A 乙酰乙酰辅酶A和CoA(转化2)(iii)乙酰乙酰辅酶A和乙酸酯与乙酰乙酸酯和乙酰辅酶A(转化3.1)(iv)乙酰乙酸酯向丙酮和CO 2(转化4)(v)丙酮和NAD(P)H 和H +与异丙醇和NAD(P)+(转化5),其中至少一个DNA分子与微生物宿主细胞是异源的,并且其中微生物宿主细胞产生异丙醇。 在另一个实施方案中,公开了用于生产异丙醇的方法,包括提供重组微生物宿主细胞,宿主细胞(i)在发酵培养基中的可发酵碳底物,其中产生异丙醇的条件下,并回收异丙醇。
    • 69. 发明申请
    • METHODS OF EXCHANGING SECURE MESSAGES
    • WO2003081377A3
    • 2003-10-02
    • PCT/US2002/040616
    • 2002-12-18
    • ESIGNX CORPORATIONWANG, Ynjiun, P.
    • WANG, Ynjiun, P.
    • G07F19/00
    • The invention enables a registered PEAD user to exchange a secure message with another registered PEAD user by using the user ID and the user public key information in the server. The sender can retrieve the public key information from the server 1201 using the receiver's user ID as an index; then the sender can derive the shared secret using the receiver's public key. The sender then can encrypt the message with the shared secret and send it over to a server with the other PEAD user's (receiver's) ID appended with the sender's user ID over the wireless network and/or Internet. The server then stores the message and forwards the message to the receiver once the receiver's PEAD is polling for messages. (It is understood in the art that the server can push the messages to the receiver's PEAD). The receiving PEAD user can use the sender's PEAD user ID and sender's public key information to derive the shared secret to decrypt a received secure message. Once a shared secret is computed or established by protocol between two users, that shared secret can be saved in the PEAD for future communication encryption/decryption usage.
    • 70. 发明申请
    • METHODS OF EXCHANGING SECURE MESSAGES
    • 交换安全信息的方法
    • WO2003081377A2
    • 2003-10-02
    • PCT/US2002/040616
    • 2002-12-18
    • ESIGNX CORPORATIONWANG, Ynjiun, P.
    • WANG, Ynjiun, P.
    • G06F
    • H04L63/0435G06F21/35G06F21/606G06Q20/02G06Q20/18G06Q20/32G06Q20/341G06Q20/3415G06Q20/363G06Q20/382G06Q20/40G06Q20/40975G07F7/0866G07F7/0886G07F7/1008G07F7/1025G07F19/20H04L63/0414H04L63/0442H04L63/0853H04L2463/061H04L2463/102
    • The invention enables a registered PEAD user to exchange a secure message with another registered PEAD user by using the user ID and the user public key information in the server. The sender can retrieve the public key information from the server 1201 using the receiver's user ID as an index; then the sender can derive the shared secret using the receiver's public key. The sender then can encrypt the message with the shared secret and send it over to a server with the other PEAD user's (receiver's) ID appended with the sender's user ID over the wireless network and/or Internet. The server then stores the message and forwards the message to the receiver once the receiver's PEAD is polling for messages. (It is understood in the art that the server can push the messages to the receiver's PEAD). The receiving PEAD user can use the sender's PEAD user ID and sender's public key information to derive the shared secret to decrypt a received secure message. Once a shared secret is computed or established by protocol between two users, that shared secret can be saved in the PEAD for future communication encryption/decryption usage.
    • 本发明使得注册的PEAD用户能够通过使用服务器中的用户ID和用户公钥信息与另一个注册的PEAD用户交换安全消息。 发送者可以使用接收者的用户ID作为索引从服务器1201检索公开密钥信息; 那么发送者可以使用接收者的公共密钥来导出共享密钥。 然后,发送者可以使用共享秘密加密消息,并将其发送到具有通过无线网络和/或因特网的发送者用户ID附加的其他PEAD用户(接收者)ID的服务器。 然后,当接收机的PEAD轮询消息时,服务器将存储消息并将消息转发给接收者。 (在本领域中可以理解,服务器可以将消息推送到接收机的PEAD)。 接收的PEAD用户可以使用发送者的PEAD用户ID和发送者的公钥信息来导出共享密钥来解密所接收的安全消息。 一旦通过两个用户之间的协议计算或建立共享密钥,该共享密钥可以保存在PEAD中以用于将来的通信加密/解密使用。