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51. 发明申请

WO9945103A3 HUMAN SPINAL CORD CELL LINES AND METHODS OF USE THEREFOR 审中-公开
标题翻译：人类脊髓细胞系及其使用方法
公开(公告)号：WO9945103A3
公开(公告)日：1999-11-25
申请号：PCT/US9904535
申请日：1999-03-02
申请人： SIGNAL PHARM INC
发明人： LI RONGHAO , SAH DINAH W Y
IPC分类号： C12N5/0797 , C12N15/12 , C12N15/22 , C12N15/33 , C12N5/10 , C12Q1/00
CPC分类号： C12N5/0623 , C12N2503/00 , C12N2510/04
摘要： Conditionally-immortalized human spinal cord cell lines are provided. Such cell lines, which may be clonal, may be used to generate neurons, including motoneurons. The cell lines and/or differentiated cells may be used for the development of therapeutic agents to prevent and treat a variety of spinal cord-related diseases and injuries. The cell lines and/or differentiated cells may also be used in assays and for the general study of spinal cord cell development and differentiation.
摘要翻译：提供有条件永生化的人脊髓细胞系。可能是克隆的这种细胞系可用于产生神经元，包括运动神经元。细胞系和/或分化细胞可用于开发用于预防和治疗各种脊髓相关疾病和损伤的治疗剂。细胞系和/或分化细胞也可用于测定和脊髓细胞发育和分化的一般研究。

52. 发明申请

WO99051721A1 NOVEL RECOMBINANT BACULOVIRUS, CONSTRUCTION METHOD THEREOF AND INSECT PESTICIDAL COMPOSITION CONTAINING THE SAME 审中-公开
标题翻译：新型重组芽孢杆菌，其构建方法和含有其的杀虫剂组合物
公开(公告)号：WO99051721A1
公开(公告)日：1999-10-14
申请号：PCT/KR1998/000338
申请日：1998-10-28
IPC分类号： C12N15/09 , A01N63/00 , C07K14/325 , C12N7/00 , C12N7/01 , C12N15/866 , C12P21/02 , A61K39/12 , C12N15/32 , C12N15/33 , C12N15/62
CPC分类号： C12N15/86 , A01N63/00 , C07K14/325 , C12N2710/14143
摘要： A recombinant baculovirus, which produces a recombinant polyhedra made up of a baculovirus polyhedrin (PH), a Bacillus thuringiensis crystal protein (CP) and jellyfish Aequorea victoria green fluorescent protein (GFP), is constructed by introducing a transfer vector carrying a fusion gene encoding a fusion protein in which the PH, the CP and the GFP are directly linked from N-terminal to C-terminal, in sequence, and a wild-type baculovirus into an insect cell, simultaneously, and culturing the cell. This baculovirus transfer vector pColorBtrus is constructed by synthesizing the GFP-coding DNA fragment from plasmid pGFP, the PH gene from wild-type Autographa californica Nucleopolyhedrovirus, and aCry1Ac gene from plasmid pPN6.6 carrying a Bacillus thuringiensis Cry1Ac gene by polymerase chain reactions, and inserting first the GFP-coding DNA fragment and the PH gene in baculovirus expression vector pAcUW31 in such a way that the 5'-end of the GFP-coding DNA fragment is linked to the 3'-end of the PH gene and then, the Cry1Ac gene between the PH gene and the GFP-coding DNA fragment. The recombinant virus can kill insect pests with high pathogenicity within a short time, and is equipped with a monitoring device for infected insects, so that it can be prevented from being overused.
摘要翻译：通过引入携带融合基因编码的转移载体构建重组杆状病毒，其由杆状病毒多角体蛋白（PH），苏云金芽孢杆菌晶体蛋白（CP）和水母Aequorea维多利亚绿色荧光蛋白（GFP）构成的重组多面体将其中PH，CP和GFP依次从N端直接连接到C末端的融合蛋白，将野生型杆状病毒同时进入昆虫细胞，并培养细胞。该杆状病毒转移载体pColorBtrus是通过聚合酶链反应合成来自质粒pGFP的GFP编码DNA片段，来自野生型苜蓿苜蓿核多角体病毒的PH基因和携带苏云金芽孢杆菌Cry1Ac基因的质粒pPN6.6的Cry1Ac基因构建的，首先在杆状病毒表达载体pAcUW31中首先插入GFP编码的DNA片段和PH基因，使GFP编码的DNA片段的5'末端连接到PH基因的3'末端，然后， PH基因与GFP编码DNA片段之间的Cry1Ac基因。重组病毒可以在短时间内杀死具有高致病性的昆虫，并配备感染昆虫的监测装置，以防止其被过度使用。

53. 发明申请

WO99050292A1 RECOMBINANT VIRUS 审中-公开
标题翻译：重组病毒
公开(公告)号：WO99050292A1
公开(公告)日：1999-10-07
申请号：PCT/GB1999/000874
申请日：1999-03-19
IPC分类号： A61K39/193 , C12N7/04 , C12N15/33 , C07K14/18
CPC分类号： C12N7/00 , A61K39/12 , A61K39/193 , A61K2039/5256 , A61K2039/53 , C12N2710/10343 , C12N2770/36122 , C12N2770/36134 , C12N2770/36162
摘要： A nucleic acid which encodes a polypeptide which produces a protective immune response against an alpha-virus such as a Venezuelan Equine Encephalitis Virus, in a mammal to which it is administered, said nucleic acid lacking a competant nuclear targeting signal in the capsid gene. The nucleic acids of the invention are expressed at enhanced levels in vectors such as adenovirus vectors. In particular there is described a deletion mutant of an alpha-virus. Vectors such as recombinant adenoviruses which express the deletion mutant are potentially of use as vaccines.
摘要翻译：编码在其所施用的哺乳动物中产生针对α病毒（例如委内瑞拉马脑炎病毒）的保护性免疫应答的多肽的核酸，所述核酸在衣壳基因中缺乏竞争性核靶向信号。本发明的核酸在载体例如腺病毒载体中以增强的水平表达。特别地，描述了α-病毒的缺失突变体。载体如表达缺失突变体的重组腺病毒可能用作疫苗。

54. 发明申请

WO99027110A1 HORMONE-DEPENDENT FORMS OF THE ADENO-ASSOCIATED VIRUS, REP PROTEINS, DNA SEQUENCES CODING FOR THEM, VECTORS CONTAINING THEM, AND REGULATORY METHODS OF THEIR INTRACELLULAR ACTIVITY 审中-公开
标题翻译：腺病毒相关病毒的HORMONE依赖形式，REP蛋白，它们的DNA序列编码，包含它们的载体及其细胞内活性的调节方法
公开(公告)号：WO99027110A1
公开(公告)日：1999-06-03
申请号：PCT/IT1998/000329
申请日：1998-11-20
IPC分类号： C12N15/09 , A61K48/00 , C07K14/075 , C07K14/72 , C07K19/00 , C12N5/10 , C12N15/33 , C12N15/864 , C12Q1/02 , C12Q1/68 , C12N15/12 , C12N15/86
CPC分类号： C12N7/00 , A61K48/00 , C07K14/005 , C07K14/721 , C07K2319/00 , C12N15/86 , C12N2710/10322 , C12N2750/14143 , C12N2750/14152
摘要： The subject matters of this invention are the hormone-dependent forms of Rep 78 and Rep 68 proteins of the Adeno-associated virus (AAV), obtained by the fusion of their specific mutants with the hormone binding domain (HBD) of steroid hormone receptors, and the DNA sequences coding for them. The invention also refers to a method for the hormonal regulation of the activity of the fusion products Rep78/68-HBD, inserted into eucaryotic cells utilising viral or non-viral systems, in order to direct the stable integration of DNA sequences in specific regions of the host human genome for therapeutic purposes. The fusion products Rep78/68-HBD are also utilised to generate viral hybrid vectors (i.e. adenovirus vectors AAV) and for generating recombinant vectors AAV.
摘要翻译：本发明的主题是通过将其特异性突变体与类固醇激素受体的激素结合结构域（HBD）融合而获得的腺相关病毒（AAV）的Rep 78和Rep 68蛋白的激素依赖性形式，和编码它们的DNA序列。本发明还涉及使用病毒或非病毒系统插入到真核细胞中的融合产物Rep78 / 68-HBD的活性调节方法，以便指导DNA序列在特定区域的稳定整合用于治疗目的的宿主人类基因组。融合产物Rep78 / 68-HBD还用于产生病毒杂交载体（即腺病毒载体AAV）和用于产生重组载体AAV。

55. 发明申请

WO98038322A1 NUCLEIC ACID CONSTRUCTS FOR DURABLE TRANSGENE EXPRESSION 审中-公开
标题翻译：核酸构建体转基因的长期持久的表达
公开(公告)号：WO98038322A1
公开(公告)日：1998-09-03
申请号：PCT/EP1998/000992
申请日：1998-02-20
IPC分类号： C12N15/33 , C12N15/38 , C12N15/63 , C12N15/85 , C12N15/79 , C12N15/67
CPC分类号： C12N15/635 , C12N15/85 , C12N2830/006 , C12N2840/203
摘要： Nucleic acid constructs suitable for expressing a foreign gene in a host cell have the following components: (a) at least part of a promotor which corresponds to a host-related, constitutive active promotor; (b) at least one binding site for a transcription factor; (c) at least one transgene; and (d) at least one gene coding for a transcription factor that can bond to the binding site for a transcription factor (b).
摘要翻译：本发明公开了核酸构建体适合于将外源基因在宿主细胞中表达，并且包括以下组件：（a）至少对应于一个与主机相关的，组成型活性启动子的启动子的一部分; （B）转录因子的至少一个结合位点; （C）至少至少一种转基因和（d）编码在（b）的能够结合到结合位点的转录因子的转录因子一种基因。

56. 发明申请

WO1998003657A1 VIRAL INTERLEUKIN-6 审中-公开
公开(公告)号：WO1998003657A1
公开(公告)日：1998-01-29
申请号：PCT/EP1996003199
申请日：1996-07-19
申请人： BEHRING DIAGNOSTICS GMBH , NEW YORK UNIVERSITY , FLECKENSTEIN, Bernhard , ALBRECHT, Jens-Christian , NEIPEL, Frank , FRIEDMAN-KIEN, Alvin , HUANG, Yao-Qi
发明人： BEHRING DIAGNOSTICS GMBH , NEW YORK UNIVERSITY
IPC分类号： C12N15/33
CPC分类号： C07K14/005 , A61K38/00 , C07K14/5412 , C07K16/085 , C12N2710/16422 , C12N2710/16622 , G01N33/6869
摘要： The present invention relates to viral interleukin-6 (v-IL-6), which can be obtained by recombinant expression of the DNA of human herpesvirus type 8 (HHV-8), and which may be used in diagnosis and treatment of human diseases such as kaposi sarcoma, Castleman's disease, multiple myeloma, kidney cell carcinoma, mesangial proliferative glomerulonephritis or B cell lymphoma.
摘要翻译：本发明涉及通过重组表达人疱疹病毒8型（HHV-8）的DNA获得的病毒白细胞介素-6（v-IL-6），其可用于人类疾病的诊断和治疗如卡波西肉瘤，卡斯尔曼氏病，多发性骨髓瘤，肾细胞癌，肾小球膜增生性肾小球性肾炎或B细胞淋巴瘤。

57. 发明申请

WO9628562A3 METHOD OF CHECKING UNDESIRED MULTIPLICATION OF VIRMETHOD OF CHECKING UNDESIRED MULTIPLICATION OF VIRUSES AND OF PREPARING VIRUS-RESISTANT ORGANISMS USES AND OF PREPARING VIRUS-RESISTANT ORGANISMS 审中-公开
标题翻译：检查未经许可的检测病毒多样化病毒和准备抗病毒有机体的方法和制备抗病毒有机体的方法
公开(公告)号：WO9628562A3
公开(公告)日：1997-03-13
申请号：PCT/EP9600989
申请日：1996-03-08
申请人： HOECHST SCHERING AGREVO GMBH , LOSS PETER , SCHNEIDER RUDOLF
发明人： LOSS PETER , SCHNEIDER RUDOLF
IPC分类号： C12N9/12 , C12N15/82 , A01H5/00 , A01N63/00 , C12N15/33 , C12N15/54
CPC分类号： C12N9/127 , C12N15/8283
摘要： The invention concerns a method of inhibiting virus multiplication by deliberate interference with the interactions necessary for virus replication by the expression of chromosomally coded dominantly negative mutations.

58. 发明申请

WO1996037616A1 ALPHAVIRUS RNA REPLICON SYSTEMS 审中-公开
公开(公告)号：WO1996037616A1
公开(公告)日：1996-11-28
申请号：PCT/US1996007454
申请日：1996-05-21
申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , THE GOVERNMENT OF THE UNITED STATES, represented by THE SECRETARY OF THE ARMY , JOHNSTON, Robert, E. , DAVIS, Nancy, L. , SMITH, Jonathan, F. , PUSHKO, Peter , PARKER, Michael , LUDWIG, George
发明人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , THE GOVERNMENT OF THE UNITED STATES, represented by THE SECRETARY OF THE ARMY
IPC分类号： C12N15/33
CPC分类号： C07K14/005 , A61K2039/5254 , C12N7/00 , C12N15/86 , C12N2770/36122 , C12N2770/36143 , C12N2770/36152 , C12N2770/36162 , C12N2840/20
摘要： The present invention provides a helper cell for expressing an infectious, replication defective, alphavirus particle in an alphavirus-permissive cell. The helper cell includes (a) a first helper RNA encoding (i) at least one alphavirus structural protein, and (ii) not encoding at least one alphavirus structural protein; and (b) a second helper RNA separate from the first helper RNA, the second helper RNA (i) not encoding the alphavirus structural protein encoded by the first helper RNA, and (ii) encoding the at least one alphavirus structural protein not encoded by the first helper RNA. Preferably, the helper cell is co-transfected with a replicon RNA encoding an alphavirus packaging segment and an inserted heterogeneous RNA, such that all of the alphavirus structural proteins assemble together into alphavirus particles in the cell, with said replicon RNA packaged therein.
摘要翻译：本发明提供了用于在甲病毒允许细胞中表达感染性，复制缺陷型甲病毒颗粒的辅助细胞。辅助细胞包括（a）编码（i）至少一种甲病毒结构蛋白的第一辅助RNA，和（ii）不编码至少一种甲病毒属结构蛋白; 和（b）与第一辅助RNA分开的第二辅助RNA，不编码由第一辅助RNA编码的甲病毒结构蛋白的第二辅助RNA（i），和（ii）编码不由第一辅助RNA编码的至少一种甲病毒结构蛋白，第一个辅助RNA。优选地，辅助细胞与编码甲病毒包装片段和插入的异质RNA的复制子RNA共转染，使得所有甲病毒属结构蛋白在一起组装成细胞中的甲病毒颗粒，其中包含所述复制子RNA。

59. 发明申请

WO9629413A2 DNA ENCODING HUMAN PAPILLOMA VIRUS TYPE 18 审中-公开
标题翻译： DNA编码人类斑马鱼病毒18型
公开(公告)号：WO9629413A2
公开(公告)日：1996-09-26
申请号：PCT/US9603649
申请日：1996-03-18
申请人： MERCK & CO INC , HOFMANN KATHRYN J , JANSEN KATHRIN U , NEEPER MICHAEL P , JOYCE JOSEPH G , GEORGE HUGH A
发明人： HOFMANN KATHRYN J , JANSEN KATHRIN U , NEEPER MICHAEL P , JOYCE JOSEPH G , GEORGE HUGH A
IPC分类号： C12N15/09 , A61K39/00 , A61K39/12 , A61K48/00 , A61P31/12 , C07K14/025 , C07K16/08 , C12N7/00 , C12N7/04 , C12N9/10 , C12N9/60 , C12N15/33 , C12N15/37 , C12N15/81 , C12N15/90 , C12P21/02 , C12R1/865 , C12R1/91
CPC分类号： C07K14/005 , A61K39/00 , C07K2319/00 , C12N7/00 , C12N9/1051 , C12N9/60 , C12N15/905 , C12N2710/20022 , C12N2710/20023
摘要： The present invention is directed to DNA molecules encoding purified human papillomavirus type 18 and derivatives thereof.
摘要翻译：本发明涉及编码18型纯化人乳头状瘤病毒的DNA分子及其衍生物。

60. 发明申请

WO1995009920A1 VIRUS RESISTANT OR TOLERANT CELLS 审中-公开
标题翻译：抗病毒或耐药细胞
公开(公告)号：WO1995009920A1
公开(公告)日：1995-04-13
申请号：PCT/EP1994003295
申请日：1994-10-05
申请人： SANDOZ LTD. , SANDOZ-PATENT-GMBH , SANDOZ ERFINDUNGEN VERWALTUNGSGESELLSCHAFT MBH , DE HAAN, Petrus, Theodorus
发明人： SANDOZ LTD. , SANDOZ-PATENT-GMBH , SANDOZ ERFINDUNGEN VERWALTUNGSGESELLSCHAFT MBH
IPC分类号： C12N15/33
CPC分类号： C07K14/005 , C12N15/8283 , C12N2770/00022
摘要： A nucleotide sequence comprising a transcriptional regulatory sequence and a sequence contiguous therewith and under the transcriptional control thereof, which contiguous sequence encodes an RNA which consists of a plurality of sub-sequences, characterized in that at least two of the sub-sequences have the sequences of viral RNAs and the RNA contains at least one translational stop codon located upstream of the 3' terminal sub-sequence. It is preferred that at least one of the sub-sequences is in an anti-sense configuration with respect to virus RNA, and that the contiguous sequence encodes mRNA. The invention also includes, inter alia, the use of such a sequence in the generation of virus resistant or tolerant plants, and such plants comprising the sequence.
摘要翻译：一种核苷酸序列，其包含转录调节序列和与其连接并在其转录控制下的序列，所述连续序列编码由多个亚序列组成的RNA，其特征在于至少两个亚序列具有序列的病毒RNA，并且RNA含有位于3'末端亚序列上游的至少一个翻译终止密码子。优选地，至少一个亚序列对于病毒RNA是反义构型，并且连续序列编码mRNA。本发明还包括这样的序列在产生抗病毒或耐受性植物中的用途，以及包含该序列的这种植物。

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