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41. 发明申请

WO2012027376A2 METHOD FOR EXPANSION OF STEM CELLS AND THE USE OF SUCH CELLS 审中-公开
标题翻译：用于扩大干细胞的方法和这种细胞的使用
公开(公告)号：WO2012027376A2
公开(公告)日：2012-03-01
申请号：PCT/US2011048819
申请日：2011-08-23
申请人： UNIV NEW YORK STATE RES FOUND , MA YUPO
发明人： MA YUPO
IPC分类号： C12N5/074 , C07K14/47 , C12N5/02 , C12N5/0789
CPC分类号： C12N5/0663 , A61K35/28 , C07K14/47 , C12N5/0647 , C12N2501/40
摘要： The present invention demonstrates that SALL4A and SALL4B are strong positive regulators of hematopoetic stem cell expansion. HSCs receiving expression of SALL4A or SALL4B are able to achieve a high- level of expansion. Cultures of SALL4-transduced cells results in extensive HSC expansion with over 1000-fold higher levels than controls within 2 to 3 weeks and expanded HSCs show no or very little maturation. Moreover, the expansion occurs quite rapidly with significant HSC growth in just a few days. In addition, SALL4-induced HSC expansion exhibits no impairment of hematopoietic cell differentiation. SALL4 appears to function in the maintanence of an undifferentiated proliferation state and block cell differentiation for HSCs.
摘要翻译：本发明证明SALL4A和SALL4B是造血干细胞扩增的强阳性调节因子。接受SALL4A或SALL4B表达的HSC能够实现高水平的扩增。 SALL4转导细胞的培养物导致广泛的HSC扩增，在2至3周内超过对照超过1000倍，并且扩增的HSC显示没有或很少的成熟。此外，在短短几天内，HSC的增长速度显着增长。此外，SALL4诱导的HSC扩增不会造成造血细胞分化的损害。 SALL4似乎在未分化增殖状态的维持和HSC的阻断细胞分化中起作用。

42. 发明申请

WO2012007725A2 METHOD OF REPROGRAMMING A CELL 审中-公开
标题翻译：细胞再生方法
公开(公告)号：WO2012007725A2
公开(公告)日：2012-01-19
申请号：PCT/GB2011001063
申请日：2011-07-14
申请人： PLASTICELL LTD , CHOO YEN , JEYAKUMAR MYLVAGANAM
发明人： CHOO YEN , JEYAKUMAR MYLVAGANAM
IPC分类号： C12N5/073
CPC分类号： C12N5/0696 , C12N2501/065 , C12N2501/385 , C12N2501/40 , C12N2501/48 , C12N2501/71 , C12N2501/72 , C12N2501/727 , C12N2501/999 , C12N2506/08 , C12N2506/1307
摘要： The present invention relates to a method of reprogramming a cell via a chemically induced multi-lineage potential (CIMP) cell comprising the steps of: (i) contacting the cell to be reprogrammed with a medium comprising one or more, preferably, two or more chemical reprogramming agents (CRAs); (ii) obtaining a CIMP cell in which the expression of one or more pluripotency genes is induced therein and one or more pluripotency proteins are expressed by the one or more induced pluripotency genes following contact with the one more CRA(s) such that the cell has gained potency and the capacity to differentiate into an increased number of cell types as compared to the cell of step (i); and (iii) differentiating the CIMP cell into a more lineage restricted cell, preferably, wherein the cell to be reprogrammed has not been genetically manipulated to induce pluripotency and/or contacted with one or more exogenous reprogramming proteins or nucleic acids encoding same to induce pluripotency.
摘要翻译：本发明涉及通过化学诱导的多谱系电位（CIMP）细胞重新编程细胞的方法，包括以下步骤：（i）使待重新编程的细胞与包含一个或多个，优选两个或更多个化学重编程（CRA）; （ii）获得其中诱导一种或多种多能性基因的表达的CIMP细胞，并且一种或多种多能性蛋白在与另一种CRA接触之后由一种或多种诱导多能性基因表达，使得细胞与步骤（i）的细胞相比，已经获得了分化为增加细胞类型数量的能力和能力; 并且（iii）将CIMP细胞分化成更谱系的限制性细胞，优选地，其中待重新编程的细胞未被遗传操作以诱导多能性和/或与一种或多种外源重编程蛋白或编码它们的核酸接触以诱导多能性。

43. 发明申请

WO2011079017A9 DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS 审中-公开
标题翻译：人胚胎干细胞的分化
公开(公告)号：WO2011079017A9
公开(公告)日：2011-12-22
申请号：PCT/US2010060756
申请日：2010-12-16
申请人： CENTOCOR ORTHO BIOTECH INC , XU JEAN
发明人： XU JEAN
IPC分类号： C12N5/071 , C12N5/0735 , C12Q1/68
CPC分类号： C12N5/0676 , C12N2501/117 , C12N2501/119 , C12N2501/16 , C12N2501/385 , C12N2501/40 , C12N2501/41 , C12N2501/415 , C12N2501/70 , C12N2501/727 , C12N2506/02 , C12N2533/90
摘要： The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon.
摘要翻译：本发明提供了促进多能干细胞分化成产胰岛素细胞的方法。具体而言，本发明提供了产生表达共表达NKX6.1和胰岛素以及最小量的胰高血糖素的胰腺内分泌谱系特征性标志物的细胞的方法。

44. 发明申请

WO2011079017A2 DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS 审中-公开
标题翻译：人胚胎干细胞的分化
公开(公告)号：WO2011079017A2
公开(公告)日：2011-06-30
申请号：PCT/US2010/060756
申请日：2010-12-16
申请人： CENTOCOR ORTHO BIOTECH INC. , XU, Jean
发明人： XU, Jean
IPC分类号： C12N5/071 , C12N5/0735 , C12Q1/68
CPC分类号： C12N5/0676 , C12N2501/117 , C12N2501/119 , C12N2501/16 , C12N2501/385 , C12N2501/40 , C12N2501/41 , C12N2501/415 , C12N2501/70 , C12N2501/727 , C12N2506/02 , C12N2533/90
摘要： The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon.
摘要翻译：本发明提供了促进多能干细胞分化成产胰岛素细胞的方法。具体而言，本发明提供了产生表达共表达NKX6.1和胰岛素和最小量的胰高血糖素的胰腺内分泌谱系特征性标志物的细胞的方法。

45. 发明申请

WO2011028880A3 METHODS FOR ENHANCING GENOME STABILITY AND TELOMERE ELONGATION IN EMBRYONIC STEM CELLS 审中-公开
标题翻译：用于增强胚胎干细胞中基因稳定性和延迟延伸的方法
公开(公告)号：WO2011028880A3
公开(公告)日：2011-06-23
申请号：PCT/US2010047644
申请日：2010-09-02
申请人： US HEALTH , KO MINORU S H , ZALZMAN MICHAL , SHAROVA LIOUDMILA V
发明人： KO MINORU S H , ZALZMAN MICHAL , SHAROVA LIOUDMILA V
IPC分类号： C12N5/0735 , C12N5/02 , C12N5/10 , C12N15/12
CPC分类号： C12N15/85 , C07K14/4705 , C12N5/00 , C12N5/0606 , C12N15/00 , C12N15/86 , C12N2501/00 , C12N2501/40 , C12N2501/60 , C12N2510/00
摘要： The disclosure provides methods for increasing genome stability of an embryonic stem (ES) cell or induced pluripotent stem (iPS) cell, increasing telomere length in an ES or iPS cell, or both, for example by contacting an ES or iPS cell with an agent that increases expression of Zscan4 in the cell. Methods for increasing the genome stability in a population of ES or iPS cells, increasing telomere length in a population of ES or iPS cells, or both, are provided, for example by selecting Zscan4+ ES or iPS cells from the population of ES or iPS cells (which can include both Zscan4+ and Zscan4- ES or iPS cells). Therapeutic methods of using ES or iPS cells expressing Zscan4 are also provided. Further provided are methods of treating cancer by administering a Zscan4 polynucleotide or Zscan4 polypeptide. Also provided are methods of inducing differentiation of isolated ES or iPS cells into germ cells.
摘要翻译：本公开提供了增加胚胎干（ES）细胞或诱导的多能干（iPS）细胞的基因组稳定性的方法，增加ES或iPS细胞或两者的端粒长度，例如通过使ES或iPS细胞与试剂接触这增加了细胞中Zscan4的表达。例如通过从ES或iPS细胞群体中选择Zscan4 + ES或iPS细胞，提供增加ES或iPS细胞群体中基因组稳定性的方法，增加ES或iPS细胞群体中的端粒长度，或两者。（其可以包括Zscan4 +和Zscan4-ES或iPS细胞）。还提供了使用表达Zscan4的ES或iPS细胞的治疗方法。还提供了通过施用Zscan4多核苷酸或Zscan4多肽来治疗癌症的方法。还提供了诱导分离的ES或iPS细胞分化为生殖细胞的方法。

46. 发明申请

WO2010111422A3 INDUCED PLURIPOTENT STEM CELL GENERATION USING TWO FACTORS AND P53 INACTIVATION 审中-公开
标题翻译：使用两个因子和P53失活诱导的PLUIPOTETI干细胞生成
公开(公告)号：WO2010111422A3
公开(公告)日：2011-03-24
申请号：PCT/US2010028541
申请日：2010-03-24
申请人： SALK INST FOR BIOLOGICAL STUDI , KAWAMURA TERUHISA , SUZUKI JOTARO , WANG YUNYUAN V , RAYA ANGEL , WAHL GEOFFREY M , IZPISUA BELMONTE JUAN CARLOS
发明人： KAWAMURA TERUHISA , SUZUKI JOTARO , WANG YUNYUAN V , RAYA ANGEL , WAHL GEOFFREY M , IZPISUA BELMONTE JUAN CARLOS
IPC分类号： C12N5/074 , C12N5/10 , C12N15/12
CPC分类号： C12N5/0696 , C12N2501/40 , C12N2501/602 , C12N2501/603 , C12N2501/998 , C12N2510/00
摘要： Methods and compositions are provided for, inter alia, the generation of induced pluripotent stem cells. Induced pluripotent stem cells may be generated by reprogramming and inhibition of p53. Further, useful intermediates for the generation of induced pluripotent stem cells are also provided.
摘要翻译：提供方法和组合物，尤其是诱导多能干细胞的产生。诱导的多能干细胞可通过重编程和抑制p53产生。此外，还提供了用于产生诱导多能干细胞的有用中间体。

47. 发明申请

WO2010055616A1 分化誘導培地用添加剤およびその利用审中-公开
标题翻译：用于差异感应培养基的添加剂及其用途
公开(公告)号：WO2010055616A1
公开(公告)日：2010-05-20
申请号：PCT/JP2009/005573
申请日：2009-10-22
申请人：独立行政法人科学技術振興機構 , 株式会社ツーセル , 国立大学法人広島大学 , 加藤幸夫 , 邵金昌 , 辻紘一郎
发明人：加藤幸夫 , 邵金昌 , 辻紘一郎
IPC分类号： C12N5/06
CPC分类号： C12N5/0654 , C12N2500/25 , C12N2500/42 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/39 , C12N2501/40 , C12N2506/1353
摘要：　幹細胞、歯髄細胞、歯根膜細胞、胎盤、羊膜および線維芽細胞からなる群より選択される１種以上の細胞を無血清条件下で骨分化誘導するための分化誘導培地用添加剤およびその利用を提供する。本発明に係る、幹細胞を無血清条件下で分化誘導するための分化誘導培地用添加剤は、ＥＧＦ、ＦＧＦ、およびＰＤＧＦからなる群より選択される１以上の増殖因子と、デキサメタゾンとβ－グリセロリン酸とを少なくとも含有する。通常アスコルビン酸２－リン酸とＩＴＳは骨分化に必須であるが、本発明の分化誘導培地用添加剤には不必要である。また、リン脂質を添加することで骨分化を促進できた。
摘要翻译：公开了一种分化诱导培养基的添加剂，用于诱导至少一种选自干细胞，牙髓细胞，牙周细胞，胎盘，羊膜和成纤维细胞的细胞分化成骨中的添加剂无血清条件。还公开了添加剂的使用。具体公开的是用于在无血清条件下诱导干细胞分化的分化诱导培养基的添加剂，其包含至少一种或多种选自EGF，FGF和PDGF的生长因子，地塞米松和β- 甘油磷酸。抗坏血酸2-磷酸和ITS通常对于分化成骨骼至关重要。然而，当使用添加剂时，这些化合物是不必要的。此外，可以通过进一步添加磷脂来促进骨分化。

48. 发明申请

WO2009091659A2 GENERATION OF TUMOR-FREE EMBRYONIC STEM-LIKE PLURIPOTENT CELLS USING INDUCIBLE RECOMBINANT RNA AGENTS 审中-公开
标题翻译：利用诱导重组RNA技术产生无肿瘤胚胎干样多功能细胞
公开(公告)号：WO2009091659A2
公开(公告)日：2009-07-23
申请号：PCT/US2009/030432
申请日：2009-01-08
申请人： LIN, Shi-lung , WU, David Ts
发明人： LIN, Shi-lung , WU, David Ts
IPC分类号： C12N15/09
CPC分类号： C12N5/0696 , C12N15/113 , C12N2310/141 , C12N2501/40 , C12N2501/65 , C12N2510/00
摘要： The present invention generally relates to a method for developing, generating and selecting tumor-free embryonic stem (ES)-like pluripotent cells using electroporation delivery of an inducible tumor suppressor mir-302 agent into mammalian cells. More particularly, the present invention relates to a method and composition for generating a Tet-On/Off recombinant transgene capable of expressing a manually re-designed mir-302 microRNA (miRNA)/shRNA agent under the control of doxycyclin (Dox) in human somatic/cancer cells and thus inducing certain specific gene silencing effects on the differentiation-associated genes and oncogenes of the cells, resulting in reprogramming the cells into an ES-like pluripotent state.
摘要翻译：本发明一般涉及使用电穿孔将可诱导肿瘤抑制剂mir-302试剂递送到哺乳动物细胞中来开发，产生和选择无肿瘤胚胎干（ES）样多能细胞的方法。更具体地说，本发明涉及用于产生能够在人类中的多西环素（Dox）控制下表达人工重新设计的mir-302微RNA（miRNA）/ shRNA试剂的Tet-On / Off重组转基因的方法和组合物体细胞/癌细胞，因此对细胞的分化相关基因和癌基因诱导某些特定的基因沉默效应，导致将细胞重编程为ES样多能状态。

49. 发明申请

WO2008127298A3 STAPHYLOCOCCAL ENTEROTOXIN B PEPTIDE COMPOSITIONS AND METHODS OF USE 审中-公开
标题翻译： STAPHYLOCOCCAL肠毒素B肽组合物及其使用方法
公开(公告)号：WO2008127298A3
公开(公告)日：2009-05-22
申请号：PCT/US2007022473
申请日：2007-10-24
申请人： CHATTERJEE SUBROTO , JETT MARTI , NEILL ROGER
发明人： CHATTERJEE SUBROTO , JETT MARTI , NEILL ROGER
IPC分类号： A61K39/00 , A61K39/38
CPC分类号： C12N5/0686 , C07K14/31 , C12N2501/05 , C12N2501/40 , C12N2501/48
摘要： The present invention relates to bacterial peptides, specifically Staphylococcus Enterotoxin B (SEB) peptides that have therapeutic use. The invention further relates to the use of SEB peptides in the diagnosis and therapy of diseases associated with cell proliferation.
摘要翻译：本发明涉及具有治疗用途的细菌肽，特别是葡萄球菌肠毒素B（SEB）肽。本发明还涉及SEB肽在诊断和治疗与细胞增殖相关的疾病中的用途。

50. 发明申请

WO2006026473A3 METHODS AND COMPOSITIONS UTILIZING MYC AND GSK3ß TO MANIPULATE THE PLURIPOTENCY OF EMBRYONIC STEM CELLS 审中-公开
标题翻译：使用MYC和GSK3β的方法和组合物来处理胚胎干细胞的分子量
公开(公告)号：WO2006026473A3
公开(公告)日：2009-04-09
申请号：PCT/US2005030488
申请日：2005-08-25
申请人： UNIV GEORGIA RES FOUND , DALTON STEPHEN , SHEPPARD ALLAN , MCLEAN CAMERON
发明人： DALTON STEPHEN , SHEPPARD ALLAN , MCLEAN CAMERON
IPC分类号： C12N5/00 , C12N5/02 , C12N5/0735
CPC分类号： C12N5/0606 , C12N2501/40 , C12N2501/70
摘要： The present invention provides methods for stabilizing pluripotent cells through the transcriptional activation of c-myc. Alternatively, the cells are stabilized through the transcriptional activation of c-myc, and the stabilization of c-myc protein levels. c-myc protein can be stabilized through the inhibition of GSK3ß or through other components of the cellular machinery that impact on c-myc stability. The invention contemplates the stabilized pluripotent cells produced using the methods described herein. Methods for the identification of compounds that modulate the stabilization of pluripotent cells through modulating transcriptional activation of c-myc, stabilization of c-myc protein levels, and/or inhibition of GSK3ß activity are also contemplated.
摘要翻译：本发明提供了通过c-myc的转录激活来稳定多能细胞的方法。或者，通过c-myc的转录激活以及c-myc蛋白水平的稳定来稳定细胞。 c-myc蛋白可以通过抑制GSK3β或通过影响c-myc稳定性的细胞机械的其他成分来稳定。本发明考虑使用本文所述的方法产生的稳定的多能细胞。还考虑了通过调节c-myc的转录激活，c-myc蛋白水平的稳定和/或GSK3β活性的抑制来鉴定调节多能细胞稳定化合物的方法。

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