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41. 发明申请

WO2016106089A1 METHODS FOR CLASSIFYING ORGANISMS BASED ON DNA OR PROTEIN SEQUENCES 审中-公开
标题翻译：基于DNA或蛋白质序列分类机制的方法
公开(公告)号：WO2016106089A1
公开(公告)日：2016-06-30
申请号：PCT/US2015/066446
申请日：2015-12-17
申请人： AGBIOME, INC.
发明人： HENRIKSEN, James, R. , TOMSO, Daniel, J.
IPC分类号： G06F19/22 , C12Q1/68
CPC分类号： G06F19/22 , C12Q1/68
摘要： Methods for classifying organisms using whole or partial genome sequence data are provided. The methods use DNA or protein sequence data and clustering algorithms to assign organisms a protein morphotype ('pmorph') based on a given clustering parameter. Pmorphs can be generated quickly from whole genome sequence data, even at draft sequence quality levels, and can resolve fine differences between related organisms. In this manner, organisms can be classified and duplicate organisms within a collection can be identified.
摘要翻译：提供了使用全部或部分基因组序列数据分类生物体的方法。该方法使用DNA或蛋白质序列数据和聚类算法根据给定的聚类参数为生物体分配蛋白质形态（'pmorph'）。即使在草稿序列质量水平，也可以从全基因组序列数据快速生成多晶型物，并且可以解决相关生物体之间的细微差异。以这种方式，可以分类生物体，并且可以鉴定集合内的重复生物体。

42. 发明申请

WO2016106066A2 PESTICIDAL GENES AND METHODS OF USE 审中-公开
公开(公告)号：WO2016106066A2
公开(公告)日：2016-06-30
申请号：PCT/US2015066314
申请日：2015-12-17
申请人： AGBIOME INC
发明人： PARKS JESSICA , ROBERTS KIRA BULAZEL , THAYER REBECCA E
IPC分类号： C07K14/325 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Polynucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

43. 发明申请

WO2016100832A1 SEQUENCES TO FACILITATE INCORPORATION OF DNA INTO THE GENOME OF AN ORGANISM 审中-公开
标题翻译：有助于将DNA合并到有机体的基因组中
公开(公告)号：WO2016100832A1
公开(公告)日：2016-06-23
申请号：PCT/US2015/066702
申请日：2015-12-18
申请人： AGBIOME, INC.
发明人： BEILINSON, Vadim , HENRIKSEN, James, R.
IPC分类号： C12N15/82
CPC分类号： C12N15/8277 , A01N57/20 , C12N9/1029 , C12N15/8209 , C12Y203/01183
摘要： Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.
摘要翻译：提供了允许宿主细胞（包括植物和植物细胞）的遗传修饰的方法和组合物。各种方法和组合物使用包含SEQ ID NO：1和/或2的重组DNA构建体或其活性变体和片段。这些多核苷酸可用于促进目的多核苷酸整合到宿主细胞（包括植物或植物细胞）的DNA中。提供载体，宿主细胞，细菌和包含重组DNA构建体或其片段的植物。还提供了将宿主细胞或植物细胞引入感兴趣的多核苷酸的方法。该方法包括使宿主细胞与能够转化宿主细胞的细菌接触，其中细菌包含含有重组DNA构建体的转化载体。

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