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1. 发明申请

WO2022245786A1 COMPOSITIONS AND METHODS FOR IMPROVING PLANT HEALTH AND CONTROLLING PLANT DISEASE 审中-公开
公开(公告)号：WO2022245786A1
公开(公告)日：2022-11-24
申请号：PCT/US2022/029579
申请日：2022-05-17
申请人： AGBIOME, INC.
发明人： BIGGS, Matthew Bryon , GACHANGO, Esther , INGHAM, David Joseph , MCCORKLE, Kestrel Lannon , TWIZEYIMANA, Mathias
IPC分类号： A01N63/20 , A01N63/22 , A01P3/00 , A01P7/04
摘要： Compositions and methods for controlling a plant pest or for treating or preventing plant disease are provided. Such compositions and methods comprise a bacterial strain that controls one or more plant pests or that improves at least one agronomic trait of interest in a plant. The bacterial strain can be used as an inoculant for plants. Methods for controlling a plant pest, for growing a plant susceptible to a plant disease, and for controlling plant disease on a plant susceptible to the plant disease are provided. Methods for improving at least one agronomic trait of interest in a plant are also provided.

2. 发明申请

WO2018191162A1 PESTICIDAL GENES AND METHODS OF USE 审中-公开
公开(公告)号：WO2018191162A1
公开(公告)日：2018-10-18
申请号：PCT/US2018/026701
申请日：2018-04-09
申请人： AGBIOME, INC.
发明人： PARKS, Jessica , ROBERTS, Kira Bulazel , THAYER, Rebecca E.
IPC分类号： C07K14/195 , A01N63/00 , C07K14/32 , C07K14/325 , C12N15/82
摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest, including microorganisms and plants. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

3. 发明申请

WO2016209708A1 PESTICIDAL GENES AND METHODS OF USE 审中-公开
公开(公告)号：WO2016209708A1
公开(公告)日：2016-12-29
申请号：PCT/US2016/037829
申请日：2016-06-16
申请人： AGBIOME, INC.
发明人： PARKS, Jessica , ROBERTS, Kira, Bulazel , THAYER, Rebecca, E.
IPC分类号： C07K14/32 , A01N63/02 , C12N15/82
CPC分类号： A01N37/18 , A01N37/46 , A01N63/02 , C07K14/32 , C12N15/8261 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Nucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

4. 发明申请

WO2016196013A2 PESTICIDAL GENES AND METHODS OF USE 审中-公开
标题翻译：杀虫基因和使用方法
公开(公告)号：WO2016196013A2
公开(公告)日：2016-12-08
申请号：PCT/US2016/033126
申请日：2016-05-18
申请人： AGBIOME, INC.
发明人： PARKS, Jessica , ROBERTS, Kira, Bulazel , THAYER, Rebecca, E.
IPC分类号： C12N15/82 , C07K14/325
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/8285 , Y02A40/162
摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Nucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.
摘要翻译：提供了具有杀虫活性的组合物及其使用方法。组合物包括具有杀虫活性的分离和重组多肽，编码所述多肽的重组和合成核酸分子，包含所述核酸分子的DNA构建体和载体，包含所述载体的宿主细胞和所述多肽的抗体。编码多肽的核苷酸序列可以用于DNA构建体或表达盒中以转化和在感兴趣的生物体中表达。提供的组合物和方法可用于生产具有增强的害虫抗性或耐受性的生物体。还提供了包含编码本发明杀虫蛋白的核苷酸序列的转基因植物和种子。这种植物对昆虫和其他害虫具有抗性。提供了用于产生本文公开的各种多肽的方法，以及使用这些多肽来控制或杀死害虫的方法。还包括用于检测样品中本发明多肽的方法和试剂盒。

5. 发明申请

WO2016100804A1 METHODS AND COMPOSITIONS FOR PROVIDING RESISTANCE TO GLUFOSINATE 审中-公开
标题翻译：提供抗葡萄糖苷的方法和组合物
公开(公告)号：WO2016100804A1
公开(公告)日：2016-06-23
申请号：PCT/US2015/066648
申请日：2015-12-18
申请人： AGBIOME, INC.
发明人： BEILINSON, Vadim , HENRIKSEN, James R. , JONES, Janice, C. , KELLY, Rebekah, Deter , SHEKITA, Amy
IPC分类号： C12N15/82 , C12N9/10
CPC分类号： C12N15/8277 , A01N57/20 , C12N9/1029 , C12N15/8209 , C12Y203/01183
摘要： Compositions and methods comprising polynucleotides and polypeptides that confer glufosinate resistance to a host cell are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the sequence that confer glufosinate resistance. Various methods of employing these sequences are provided. Such methods include, for example, methods for producing a host cell, plant, plant cell, explant or seed having glufosinate resistance, and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
摘要翻译：提供了包含赋予宿主细胞的草铵膦抗性的多核苷酸和多肽的组合物和方法。还提供了具有赋予草铵膦抗性的序列的核酸构建体，宿主细胞，植物，植物细胞，外植体，种子和谷粒。提供了使用这些序列的各种方法。这样的方法包括例如用于产生宿主细胞，植物，植物细胞，外植体或具有草铵膦抗性的种子的方法，以及在含有使用本文公开的植物和/或种子的作物的田地中控制杂草的方法。

6. 发明申请

WO2015105993A1 HIGH THROUGHPUT DISCOVERY OF NEW GENES FROM COMPLEX MIXTURES OF ENVIRONMENTAL MICROBES 审中-公开
标题翻译：来自环境微生物复合混合物的新基因的高通量发现
公开(公告)号：WO2015105993A1
公开(公告)日：2015-07-16
申请号：PCT/US2015/010648
申请日：2015-01-08
申请人： AGBIOME, INC.
发明人： BEILINSON, Vadim , JONES, Janice , PARKS, Jessica , THAYER, Rebecca E. , TOMSO, Daniel J. , UKNES, Scott Joseph , VOLRATH, Sandy , WARD, Eric Russell
IPC分类号： C12Q1/68
CPC分类号： C12N15/1065 , C12Q1/6806 , C12Q1/6827 , C12Q1/6869
摘要： Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental microbes.
摘要翻译：提供了用于分离已知基因序列的新变体的组合物和方法。该方法用于鉴定复杂混合物中的变体，特别是同系物。组合物包括与感兴趣的基因家族特别是农业兴趣杂交的杂交诱饵，以便从复杂混合物中选择性富集感兴趣的多核苷酸。诱饵序列可能对来自不同感兴趣的基因家族的许多基因是特异性的，并且可以被设计为将每个感兴趣的基因覆盖至少2倍。因此，本文公开的方法涉及寡核苷酸杂交基因捕获方法，用于鉴定来自环境样品的新的感兴趣的基因。这种方法绕过了对劳动密集型微生物菌株分离的需要，允许同时发现来自感兴趣的多个基因家族的基因，并增加了发现存在于环境微生物复杂混合物中的低丰度和不可培养生物的基因的潜力。

7. 发明申请

WO2022225926A1 COMPOSITIONS AND METHODS FOR IMPROVING PLANT HEALTH AND CONTROLLING PLANT DISEASE 审中-公开
公开(公告)号：WO2022225926A1
公开(公告)日：2022-10-27
申请号：PCT/US2022/025346
申请日：2022-04-19
申请人： AGBIOME, INC.
发明人： SPADAFORA, Vincent James , WALKER, Scott Louis
IPC分类号： A01N63/22 , A01N3/00 , A01P3/00 , A23B7/154 , A01N63/27 , C12N1/20
摘要： Compositions and methods for controlling a plant pest or for treating or preventing plant disease are provided. Such compositions and methods comprise a bacterial strain that controls one or more plant pests or that improves at least one agronomic trait of interest in a plant. The bacterial strain can be used as an inoculant for plants. Methods for controlling a plant pest, for growing a plant susceptible to a plant disease, and for controlling plant disease on a plant susceptible to the plant disease are provided. Methods for improving at least one agronomic trait of interest in a plant are also provided.

8. 发明申请

WO2022225925A1 COMPOSITIONS AND METHODS FOR IMPROVING PLANT HEALTH AND CONTROLLING PLANT DISEASE 审中-公开
公开(公告)号：WO2022225925A1
公开(公告)日：2022-10-27
申请号：PCT/US2022/025345
申请日：2022-04-19
申请人： AGBIOME, INC.
发明人： SPADAFORA, Vincent James , WALKER, Scott Louis
IPC分类号： A01N63/22 , A01P3/00 , C12N1/20
摘要： Compositions and methods for controlling a plant pest or for treating or preventing plant disease are provided. Such compositions and methods comprise a bacterial strain that controls one or more plant pests or that improves at least one agronomic trait of interest in a plant. The bacterial strain can be used as an inoculant for plants. Methods for controlling a plant pest, for growing a plant susceptible to a plant disease, and for controlling plant disease on a plant susceptible to the plant disease are provided. Methods for improving at least one agronomic trait of interest in a plant are also provided.

9. 发明申请

WO2019241370A1 COMPOSITIONS AND METHODS FOR IMPROVING PLANT HEALTH AND CONTROLLING PLANT DISEASE 审中-公开
公开(公告)号：WO2019241370A1
公开(公告)日：2019-12-19
申请号：PCT/US2019/036744
申请日：2019-06-12
申请人： AGBIOME, INC.
发明人： SIMMONS, Lee J.
IPC分类号： A01N25/00 , A01N37/24 , A01N63/00 , A01P21/00 , A01P3/00 , A01P13/00 , A01P7/00
摘要： Compositions and methods for treating or preventing plant disease are provided. Such compositions and methods comprise a combination of a ketoreductase inhibitor fungicide and a bacterial strain that control one or more pathogens that cause plant disease or improve at least one agronomic trait of interest in a plant. The combination of the ketoreductase inhibitor fungicide and bacterial strain can be used as an inoculant for plants. Therefore, methods for growing a plant susceptible to a plant disease and methods for controlling plant disease on a plant susceptible to the plant disease are provided.

10. 发明申请

WO2019204584A1 PESTICIDAL PROTEINS AND METHODS OF USE 审中-公开
公开(公告)号：WO2019204584A1
公开(公告)日：2019-10-24
申请号：PCT/US2019/028090
申请日：2019-04-18
申请人： AGBIOME, INC.
发明人： PARKS, Jessica , ROBERTS, Kira Bulazel , THAYER, Rebecca E.
IPC分类号： C12N15/82 , C07K14/195 , C07K14/325
摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

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