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31. 发明申请

WO2005075661A2 METHOD FOR TESTING OR SCREENING FOR AN ENZYME OF INTEREST 审中-公开
标题翻译：测试或筛选感兴趣酶的方法
公开(公告)号：WO2005075661A2
公开(公告)日：2005-08-18
申请号：PCT/DK2005000084
申请日：2005-02-09
申请人： NOVOZYMES AS , BJOERNVAD MADS ESKELUND
发明人： BJOERNVAD MADS ESKELUND
IPC分类号： C12Q1/28 , C12Q1/54 , C12Q1/00
CPC分类号： C12Q1/28 , C12Q1/54
摘要： The present invention relates to methods for testing or screening for an enzyme of interest.
摘要翻译：本发明涉及测试或筛选感兴趣的酶的方法。

32. 发明申请

WO2004108009A3 METHOD AND KIT FOR ASSESSING RISK FOR BOVINE RESPIRATORY DISEASE 审中-公开
标题翻译：用于评估BOVINE呼吸道疾病风险的方法和工具包
公开(公告)号：WO2004108009A3
公开(公告)日：2005-08-04
申请号：PCT/US2004017826
申请日：2004-06-07
申请人： OXIS INT INC , BUCK ROBERT
发明人： BUCK ROBERT
IPC分类号： A61D20060101 , C12Q1/26 , C12Q1/28 , G01N33/53
CPC分类号： C12Q1/28
摘要： The present invention provides methods and kits useful for evaluating, profiling, screening and managing an animal or animal population for susceptibility to a condition or disease. Additionally, the present invention extends to methods for modulating the treatment of a population of animals, monitoring the efficacy of treatment protocols for such disease and inhibiting the spread of a disease in a population. The methods according to the invention are particularly useful for the cattle industry to determine susceptibility to Bovine Respiratory Disease ("BRD") among cattle and to enable dividing a cattle population into risk groups for differential treatment and assessment.
摘要翻译：本发明提供了可用于评价，分析，筛选和管理动物或动物群体对病症或疾病易感性的方法和试剂盒。此外，本发明还涉及用于调节动物群体治疗的方法，监测这种疾病的治疗方案的功效并抑制群体中疾病的传播。根据本发明的方法对牛业尤其有用，以确定牛中牛类呼吸道疾病（“BRD”）的易感性，并且能够将牛群体分为风险群体进行差别待遇和评估。

33. 发明申请

WO2004076054A2 DRY CHEMISTRY, LATERAL FLOW-RECONSTITUTED CHROMATOGRAPHIC ENZYME-DRIVEN ASSAYS 审中-公开
标题翻译：干化学，横向流动重建的色谱酶解试验
公开(公告)号：WO2004076054A2
公开(公告)日：2004-09-10
申请号：PCT/US2004005319
申请日：2004-02-24
申请人： BINAX INC
发明人： PIASIO ROGER , TURNER NATHAN
IPC分类号： B01J20060101 , B01J2/00 , C12M1/34 , C12N1/06 , C12N9/99 , C12Q1/28 , C12Q1/32 , C12Q1/37 , C12Q1/42 , C12Q1/48 , C12Q1/54 , C12Q1/60 , G01N21/00 , G01N31/22 , G01N33/543 , G01N33/558 , B01J
CPC分类号： C12Q1/32 , B01J20/28033 , B01J20/281 , G01N21/78 , G01N33/523 , G01N33/54326 , G01N33/558 , G01N2333/902 , G01N2333/904 , G01N2333/986
摘要： A lateral flow chromatographic assay format for the performance of rapid enzyme-driven assays is described. A combination of components necessary to elicit a specific enzyme reaction, which are either absent from the intended sample or insufficiently present therein to permit completion of the desired reaction, are predeposited as substrate in dry form together with ingredients necessary to produce a desired color upon occurrence of the desired reaction. The strip is equipped with a sample pad placed ahead of the substrate deposit in the flow stream, to which liquid sample is applied. The sample flows from the sample pad into the substrate zone where it immediately reconstitutes the dried ingredients while also intimately mixing with them and reacting with them at the fluid front. The fluid front moves rapidly into the final "read zone" wherein the color developed is read against predetermined color standards for the desired reaction. Pretreatment pads for the sample, as needed, (e.g. a lysing pad for lysing red blood cells in whole blood) are placed in front of the sample pad in the flow path as appropriate. The assay in the forma of the invention is faster and easier to perform than analogous wet chemistry assays. Specific assay for glucose-6-phosphate dehydrogenase ("G-6PD"), total serum cholesterol, beta-lactamase activity and peroxidase activity are disclosed
摘要翻译：描述了用于执行快速酶驱动测定的横向流动色谱测定格式。引发特定酶反应所需的组分的组合（其不是预期样品中所含的或其中不充分存在以允许完成所需反应）所需的组分的组合预先沉积为干燥形式的底物以及在出现时产生所需颜色的期望的反应。该条带配备有样品垫，该样品垫放置在流动物流中的基材沉积物的前方，液体样品被施加到该样品垫上。样品从样品垫流入基材区域，在那里它立即重新形成干燥的成分，同时与它们紧密混合并在流体前沿与它们反应。流体前沿迅速移动到最终的“读取区域”，在该读取区域中，所展开的颜色是针对所需反应的预定颜色标准读取的。根据需要，用于样品的预处理垫（例如用于溶解全血中的红细胞的溶解垫）适当地放置在流动路径中的样品垫的前面。本发明形式中的测定比类似的湿化学测定更快且更容易执行。公开了葡萄糖-6-磷酸脱氢酶（“G-6PD”），总血清胆固醇，β-内酰胺酶活性和过氧化物酶活性的具体测定

34. 发明申请

WO03025212A3 METHOD FOR DETECTING SEPSIS 审中-公开
标题翻译：检测SEPSIS的方法
公开(公告)号：WO03025212A3
公开(公告)日：2003-09-12
申请号：PCT/US0228211
申请日：2002-09-05
申请人： UNIV WASHINGTON , HEINECKE JAY W
发明人： HEINECKE JAY W
IPC分类号： G01N27/62 , C12Q1/02 , G01N30/02 , G01N30/72 , G01N30/88 , G01N33/50 , G01N33/53 , G01N33/68 , C12Q1/28 , C12Q1/04
CPC分类号： G01N33/6812 , G01N30/7206 , G01N33/5308 , G01N2030/025 , Y10S436/811 , Y10T436/173845 , Y10T436/24
摘要： The disclosure describes a method for detecting conditions indicative of sepsis. In one embodiment of the invention, an increase in the level of 3-chlorotyrosine or 3-bromotyrosine from the normal level in a sample of body fluid or tissue is indicative of early sepsis or infection. In another embodiment of the invention, the level of 3-chlorotyrosine or 3-bromotyrosine is measured or monitored to determine the response to therapeutic treatment of the infective condition in which a reductuion in the level that existed prior to the treatment is an early sign or indication that the treatment is working in vivo. In a preferred embodiment, the method of the invention is illustrated in a clinically relevant mouse model of sepsis.
摘要翻译：本公开描述了一种用于检测指示败血症的病症的方法。在本发明的一个实施方案中，体液或组织样品中3-氯酪氨酸或3-溴酪氨酸水平从正常水平的增加指示早期败血症或感染。在本发明的另一个实施方案中，测量或监测3-氯酪氨酸或3-溴酪氨酸的水平以确定治疗性治疗对感染状况的反应，其中在治疗前存在的水平的还原是早期征兆或表明治疗在体内工作。在优选的实施方案中，本发明的方法在临床相关的败血症小鼠模型中进行了说明。

35. 发明申请

WO03002733A2 COMPOSITIONS FOR ENHANCED CATALYZED REPORTER DEPOSITION 审中-公开
标题翻译：强化催化剂沉积的组合物
公开(公告)号：WO03002733A2
公开(公告)日：2003-01-09
申请号：PCT/US0220460
申请日：2002-06-28
申请人： PERKINELMER LIFE SCIENCES INC
发明人： ADLER KARL EDWIN JR
IPC分类号： C12N9/96 , C12Q1/28 , G01N21/76 , G01N33/53 , G01N33/531
CPC分类号： C12N9/96 , C12Q1/28 , Y10S435/968 , Y10S435/975
摘要： A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol-containing molecule with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent comprising an organic compound having the structure of Formula (I) wherein each R is independently selected from the group consisting of: hydrogen and a C1-12 sustituent; where V, W, Y, and Z are each independently selected from the group consisting of: H, halogen, the C1-12 substituent, NR2, OR and SR; and where X is selected from the group consisting of: H, Br, Cl, F, the C1-12 substituent, NR2, OR and SR; or synergistic mixtures of an inorganic salt and the organic compound. The C1-12 substituent is linear, branched or cyclic. The C1-12 substituent is alkyl, alkenyl, alkynyl, heteroatom substituted alkyl, heteroatom substituted alkenyl, heteroatom substituted alkynyl, aryl, arylalkyl, arylalkenyl or arylalkynyl. Further, the heteroatom is N, O, S or halogen. Any C, N, O or S in the C1-12 substituent optionally has a pendant moiety which is carbonyl, hydroxyl, carboxyl, amine, thiol, thioester, thioether, phosphate, alkoxy, aryl, arylalkyl, sulfonamide or alkyl halide. A kit is provided containing an enhancing reagent for enhancing the detection of an enzyme reaction as described herein, together with instructions for use.
摘要翻译：用于增强通过过氧化物酶将苯酚底物转化成产物的方法包括以下步骤：在增强剂存在下，使包含可检测标记的含酚分子的缀合物与过氧化物酶反应，所述增强剂包含有机具有式（I）结构的化合物，其中每个R独立地选自：氢和C1-12取代基; 其中V，W，Y和Z各自独立地选自：H，卤素，C1-12取代基，NR2，OR和SR; 并且其中X选自：H，Br，Cl，F，C1-12取代基，NR2，OR和SR; 或无机盐和有机化合物的协同混合物。 C1-12取代基是直链，支链或环状的。 C1-12取代基是烷基，烯基，炔基，杂原子取代的烷基，杂原子取代的烯基，杂原子取代的炔基，芳基，芳基烷基，芳基烯基或芳基炔基。此外，杂原子是N，O，S或卤素。 C1-12取代基中的任何C，N，O或S任选具有羰基，羟基，羧基，胺，硫醇，硫酯，硫醚，磷酸酯，烷氧基，芳基，芳基烷基，磺酰胺或烷基卤的侧基部分。提供了包含用于增强如本文所述的酶反应检测的增强试剂以及使用说明的试剂盒。

36. 发明申请

WO0111359A9 COLOR SPACE ANALYSIS IN BIOCHEMICAL AND IMMUNOLOGICAL ASSAYS 审中-公开
标题翻译：生物化学和免疫测定中的颜色空间分析
公开(公告)号：WO0111359A9
公开(公告)日：2002-08-29
申请号：PCT/CA0000918
申请日：2000-08-04
申请人： IMI INTERNAT MEDICAL INNOVATIO , EVELEGH MICHAEL J
发明人： EVELEGH MICHAEL J
IPC分类号： G01J3/46 , C12Q1/26 , C12Q1/28 , C12Q1/54 , C12Q1/60 , G01N21/27 , G01N21/78 , G01N33/483 , G01N33/52 , G01N33/574 , G01N33/68 , G01N33/92
CPC分类号： G01N33/6803 , C12Q1/54 , C12Q1/60 , G01N33/5005 , G01N33/5091 , G01N33/52 , G01N33/57484 , G01N33/68 , G01N33/92
摘要： A process is provided for analyzing a specimen of biological material in any of a number of biochemical or immunological tests for an analyte which involves subjecting the specimen to treatment which develops a color correlating to the amount of analyte in the specimen. According to the invention at least one defined color characteristic selected from hue angle, chroma, saturation and lightness of the developed color is measured and the results of that measurement analyzed to determine the presence or concentration of the analyte in the specimen. Particular applications are to the detection of cancerous or pre-cancerous abnormalities from the analysis of lung mucus, throat mucus, cervical mucus or seminal fluid.
摘要翻译：提供了一种用于分析生物材料样本的方法，其中任何一种生物化学或免疫学测试用于分析物，其包括对样品进行处理，所述处理产生与样品中分析物的量相关的颜色。根据本发明，测量从显色度的色调角，色度，饱和度和亮度中选出的至少一个定义的颜色特征，并分析该测量结果以确定样品中分析物的存在或浓度。具体应用是从肺粘液，咽喉粘液，宫颈粘液或精液分析中检测癌症或癌前异常。

37. 发明申请

WO0132916A3 AUTOMATED LYSOPHOSPHOLIPID ASSAY AND METHODS OF DETECTING CANCER 审中-公开
标题翻译：自动化石蜡蛋白测定法及检测癌症的方法
公开(公告)号：WO0132916A3
公开(公告)日：2002-07-11
申请号：PCT/US0030280
申请日：2000-11-02
申请人： ABBOTT LAB
发明人： RUSSELL JOHN C , GRANADOS EDWARD N
IPC分类号： G01N21/78 , C12Q1/26 , C12Q1/28 , C12Q1/32 , C12Q1/37 , C12Q1/44 , C12Q1/48 , C12Q1/527
CPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/32 , C12Q1/44 , G01N2405/04
摘要： The present invention relates to an improved enzymatic diagnostic assay to detect carcinoma by measuring various lysophospholipids, including lysophosphatidic acid (LPA), in a patient. In a preferred embodiment, this assay measures the human plasma level of LPA in an automated format with a minimal number of reagents and with reduced incubation periods. The present invention also comprises several additional technical improvements to the current LPA assays disclosed in the prior art.
摘要翻译：本发明涉及通过测量患者中各种溶血磷脂（包括溶血磷脂酸（LPA））来检测癌症的改进的酶学诊断测定法。在优选的实施方案中，该测定法以最小数量的试剂和减少的潜伏期以自动化形式测量LPA的人血浆水平。本发明还包括对现有技术中公开的当前LPA测定法的若干附加技术改进。

38. 发明申请

WO02037109A2 DETECTION OF BINDING SPECIES WITH COLLOIDAL AND NON-COLLOIDAL STRUCTURES 审中-公开
标题翻译：用胶结和非胶结构检测结合物种
公开(公告)号：WO02037109A2
公开(公告)日：2002-05-10
申请号：PCT/US2001/044964
申请日：2001-11-01
IPC分类号： G01N33/53 , C12Q1/28 , G01N21/27 , G01N21/78 , G01N27/27 , G01N27/327 , G01N33/483 , G01N33/543 , G01N33/566 , G01N33/58 , C12Q1/68
CPC分类号： B82Y30/00 , G01N33/54313 , G01N33/585 , G01N33/586
摘要： The invention provides novel techniques for determining the interaction between binding partners. The first binding partner is immobilized to a surface or a colloid and a second binding partner immobilized to a surface or a colloid. Non-specific binding techniques, including a SAM, provide for the immobilization of a wide variety of species. A mixture may be separated into various componens and these components may then be fastened to either the colloids or the surfaces. Binding interaction between the binding partners may be determined through a variety of assays.
摘要翻译：本发明提供用于确定结合配偶体之间相互作用的新技术。第一结合配偶体固定在固定在表面或胶体上的表面或胶体和第二结合配偶体上。包括SAM在内的非特异性结合技术提供了多种物种的固定。混合物可以分成各种组分，然后这些组分可以被固定到胶体或表面。结合配偶体之间的结合相互作用可以通过多种测定来确定。

39. 发明申请

WO0134831A3 DETERMINATION OF CATALASE ACTIVITY 审中-公开
标题翻译：催化剂活性的测定
公开(公告)号：WO0134831A3
公开(公告)日：2002-03-21
申请号：PCT/GB0004305
申请日：2000-11-10
申请人： SMITHKLINE BEECHAM PLC , SMITHKLINE BEECHAM CORP , BRIEN PETER JAMES O , SLAUGHTER MARK RAYMOND
发明人： O'BRIEN PETER JAMES , SLAUGHTER MARK RAYMOND
IPC分类号： C12Q1/28 , C12Q1/30 , C12Q1/00
CPC分类号： C12Q1/30 , C12Q1/28 , C12Q2326/00
摘要： An assay for catalase in a test sample comprising contacting the test sample with enzymatically produced H2O2, a peroxidase and a chromogenic dye; and measuring the change in color intensity.
摘要翻译：测试样品中的过氧化氢酶测定，包括使测试样品与酶促产生的H 2 O 2，过氧化物酶和显色染料接触; 并测量颜色强度的变化。

40. 发明申请

WO0055358A9 4-(4-HYDROXYSTYRYL)PYRIDINE-CONTAINING SUBSTRATES FOR AN ANALYTE DEPENDENT ENZYME ACTIVATION SYSTEM 审中-公开
标题翻译：用于分析酶依赖性酶活性体系的4-（4-羟基甲硅烷基）含吡啶基质
公开(公告)号：WO0055358A9
公开(公告)日：2002-01-03
申请号：PCT/US0007229
申请日：2000-03-17
申请人： NEN LIFE SCIENCE PROD INC
发明人： BOBROW MARK NORMAN , ROTH KEVIN AARON
IPC分类号： C07D213/30 , C07D495/04 , C12N9/08 , C12Q1/28 , G01N33/543 , C07D213/06
CPC分类号： C07D213/30 , C07D495/04 , C12Q1/28 , G01N33/54306 , Y10S435/96 , Y10T436/25375
摘要： 4-(4-hydroxystyryl) pyridine-containing compounds are disclosed as substrates for use in assays. Also, a method for detecting or quantitating an analyte is provided which includes reacting an enzyme with a 4-(4-hydroxystyryl) pyridine-containing compound to form a reactive intermediate wherein the reactive intermediate deposits covalently on a surface by binding to a receptor, wherein deposited reactive intermediates either directly or indirectly through a label generate a signal which can be detected or quantitated.
摘要翻译：公开了含有4-（4-羟基苯乙烯基）吡啶的化合物作为用于测定的底物。另外，提供了一种用于检测或定量分析物的方法，其包括使酶与含有4-（4-羟基苯乙烯基）吡啶的化合物反应以形成反应性中间体，其中反应性中间体通过结合受体共价沉积在表面上，其中直接或间接通过标签沉积的反应性中间体产生可以被检测或定量的信号。

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