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31. 发明申请

WO0218543A3 FERMENTATION PROCESS FOR THE PREPARATION OF L-THREONINE 审中-公开
标题翻译：用于制备L-苏氨酸的发酵方法
公开(公告)号：WO0218543A3
公开(公告)日：2003-01-03
申请号：PCT/EP0108603
申请日：2001-07-25
申请人： DEGUSSA
发明人： HERMANN THOMAS , RIEPING MECHTHILD
IPC分类号： C12N1/21 , C12P13/08
CPC分类号： C12P13/08
摘要： The invention relates to a process for the fermentative preparation of L-threonine in which an L-threonine-producing microorganism of the Enterobacteriaceae family is cultured by the feed process, and a portion of the fermentation broth is then separated off in order to be utilized for inoculation of further media.
摘要翻译：本发明涉及一种L-苏氨酸的发酵制备方法，其中通过进料方法培养产生肠杆菌科的L-苏氨酸生产微生物，然后将一部分发酵液分离出来以便被利用用于接种其他媒体。

32. 发明申请

WO02081722A2 PROCESS FOR THE PRODUCTION OF L-AMINO ACIDS USING STRAINS OF THE FAMILY ENTEROBACTERIACEAE THAT CONTAIN AN ATTENUATED ACEA GENE 审中-公开
标题翻译：使用含有灭活性ACEA基因的家禽肠杆菌菌株生产L-氨基酸的方法
公开(公告)号：WO02081722A2
公开(公告)日：2002-10-17
申请号：PCT/EP0202421
申请日：2002-03-06
申请人： DEGUSSA
发明人： RIEPING MECHTHILD , HERMANN THOMAS
IPC分类号： C07K14/245 , C12N15/31 , C12P13/04 , C12P13/08
CPC分类号： C07K14/245 , C12P13/04 , C12P13/08
摘要： The invention relates to a process for the production of L-amino acids, in particular L-threonine, in which the following steps are carried out: a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which the aceA gene or nucleotide sequences coding therefor are attenuated, in particular are switched off; b) enrichment of the L-amino acid in the medium or in the cells of the bacteria; and c) isolation of the L-amino acid.
摘要翻译：本发明涉及生产L-氨基酸，特别是L-苏氨酸的方法，其中进行以下步骤：a）产生所需L-氨基酸的肠杆菌科的微生物发酵，其中 aceA基因或其编码的核苷酸序列被衰减，特别是被关闭; b）在培养基或细菌细胞中富集L-氨基酸; 和c）L-氨基酸的分离。

33. 发明申请

WO0222843A3 NUCLEOTIDE SEQUENCES WHICH CODE FOR THE DEP34 GENE 审中-公开
标题翻译：为DEP34基因编码的核苷酸序列
公开(公告)号：WO0222843A3
公开(公告)日：2002-07-11
申请号：PCT/EP0109313
申请日：2001-08-11
申请人： DEGUSSA
发明人： FARWICK MIKE , HUTHMACHER KLAUS , PFEFFERLE WALTER , HERMANN THOMAS , BATHE BRIGITTE
IPC分类号： C07K14/34 , C12N1/21 , C12N15/31 , C12P13/08 , C12N15/63 , C12Q1/68
CPC分类号： C07K14/34 , C12P13/08
摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the dep34 gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译：本发明涉及分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.的氨基酸序列的多肽的多核苷酸至少70％相同的程度的多核苷酸 2的多核苷酸，b）编码包含与SEQ ID No.2的氨基酸序列的至少70％的同一性的氨基酸序列的多肽的多核苷酸，c）与SEQ ID No.2的多核苷酸互补的多核苷酸，）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少部分基因以减毒形式存在，并且使用包含根据本发明的序列作为杂交探针的多核苷酸。

34. 发明申请

WO0222632A3 NUCLEOTIDE SEQUENCES CODING FOR THE PKND GENE 审中-公开
标题翻译： PKND基因的核苷酸序列编码
公开(公告)号：WO0222632A3
公开(公告)日：2002-06-13
申请号：PCT/EP0110210
申请日：2001-09-05
申请人： DEGUSSA
发明人： BATHE BRIGITTE , SCHROEDER INDRA , FARWICK MIKE , HERMANN THOMAS
IPC分类号： C12N1/21 , C12N9/12 , C12N15/31 , C12P13/04 , C12P13/08 , C12Q1/68
CPC分类号： C12N9/1205 , C12P13/04 , C12P13/08 , C12R1/15 , C12Y207/01037
摘要： The invention relates to an isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) a polynucleotide which is complementary to the polynucleotides of a) or b), and d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要翻译：本发明涉及含有选自以下的多核苷酸序列的分离的多核苷酸：a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用其中至少pknD基因被扩增的棒状杆菌来制备L-氨基酸的发酵方法，以及用于作为含有根据本发明的序列的多核苷酸的杂交探针。

35. 发明申请

WO0218427A3 NUCLEOTIDE SEQUENCES WHICH CODE FOR THE CITA GENE 审中-公开
标题翻译： CITA基因编码的核苷酸序列
公开(公告)号：WO0218427A3
公开(公告)日：2002-05-16
申请号：PCT/EP0107766
申请日：2001-07-06
申请人： DEGUSSA
发明人： MOECKEL BETTINA , FARWICK MIKE , HERMANN THOMAS , MARX ACHIM , PFEFFERLE WALTER
IPC分类号： A61K38/48 , C07K14/34 , C12N9/12 , C12P13/08 , C12N15/10 , C12N15/63 , C12Q1/68
CPC分类号： C12N9/1205 , C07K14/34 , C12N9/10 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12P13/14 , C12P13/20 , C12P13/222 , C12P13/225 , C12P13/227 , C12P13/24
摘要： The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译：本发明涉及分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70％ b）编码多肽的多核苷酸，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）的多核苷酸互补的多核苷酸，或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少有citA基因以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

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