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    • 34. 发明申请
    • NUCLEIC ACID EXPRESSION CONSTRUCT AND ITS USE AS A CELL PROLIFERATION MARKER
    • 核酸表达结构及其作为细胞增殖标记物的用途
    • WO2011006962A1
    • 2011-01-20
    • PCT/EP2010/060208
    • 2010-07-15
    • RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITÄT BONNFLEISCHMANN, Bernd K.HESSE, MichaelRAULF, Alexandra
    • FLEISCHMANN, Bernd K.HESSE, MichaelRAULF, Alexandra
    • C12Q1/68C12N15/85G01N33/50
    • C07K14/4738C12N15/63
    • In a first aspect, the present invention provides a nucleic acid expression construct encoding a fusion protein comprising a reporter protein and a protein with a wild-type destruction signal, wherein the sequence encoding the fusion protein is operably linked to a non-endogenous promoter, and wherein the fusion protein localizes during cell cycle progression to subcellular structures selected from the group consisting of the cell cortex, the contractile ring, and the midbody. In a further aspect, the present invention provides a method of visualizing cell cycle progression, In another aspect, the present Invention provides the use of the nucleic acid expression construct of the present invention as a cell proliferation marker in vivo and in vitro. In another aspect, the present invention provides the use of the nucleic acid expression construct of the present invention to distinguish between cell division and acytokinetic mitosis and/or endoreduplication in vivo or in vitro. In yet another aspect, the present invention provides a transgenic animal comprising the nucleic acid expression of the present invention as well as its use for analyzing cell proliferation in vivo.
    • 在第一方面,本发明提供编码包含报道蛋白和具有野生型破坏信号的蛋白质的融合蛋白的核酸表达构建体,其中编码融合蛋白的序列可操作地连接于非内源启动子, 并且其中所述融合蛋白在细胞周期进展期间定位于选自细胞皮质,收缩环和中间体的亚细胞结构中。 另一方面,本发明提供了一种可视化细胞周期进程的方法。另一方面,本发明在体内和体外提供本发明的核酸表达构建体作为细胞增殖标记物的用途。 在另一方面,本发明提供了本发明的核酸表达构建体在体内或体外区分细胞分裂和动力学有丝分裂和/或内切复制的用途。 另一方面,本发明提供了包含本发明的核酸表达的转基因动物以及其在体内分析细胞增殖的用途。