会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 25. 发明申请
    • IMMUNOASSAY
    • 免疫分析
    • WO2009148406A1
    • 2009-12-10
    • PCT/SG2008/000205
    • 2008-06-06
    • AGENCY FOR SCIENCE TECHNOLOGY AND RESEARCHGAO, ZhiqiangZHANG, Jie
    • GAO, ZhiqiangZHANG, Jie
    • G01N33/543G01N33/553
    • G01N33/5438G01N33/54306
    • The invention provides a highly sensitive immunoassay for detection of a biological species. The immunoassay comprises exposing an electrode to an analyte liquid putatively containing the biological species so as to couple the biological species, if present in the analyte liquid, to a binding antibody on the electrode. The electrode comprises a binding antibody and an anchor group, each being coupled to an electrically conductive substrate, said binding antibody being capable of binding to the biological species and said anchor group being capable of binding to a redox polymer. The electrode is then exposed to an antibody-enzyme liquid comprising an antibody-enzyme species, said antibody-enzyme species comprising a detection antibody capable of binding to the biological species, said detection antibody being coupled to a redox enzyme, whereby, if the analyte liquid comprises the biological species, the redox enzyme couples to the electrode by means of the coupling of both the detection antibody and the binding antibody to the biological species. The electrode is then exposed to a polymer solution comprising the redox polymer and to an enzyme substrate, whereby if the redox enzyme is coupled to the anchor group on the electrode the redox polymer is reduced and couples to the anchor group on the electrode. A voltage is then applied between the electrode and a reference electrode and the electrode is exposed to an oxidisable species, whereby a magnitude of an electric current between said electrode and a reference electrode is indicative of the presence or absence of the biological species.
    • 本发明提供用于检测生物物种的高度灵敏的免疫测定。 免疫测定包括将电极暴露于假设含有生物物质的分析物液体,以将生物物质(如果存在于分析物液体中)与电极上的结合抗体相连接。 电极包含结合抗体和锚定基团,每个锚定基团与导电底物偶联,所述结合抗体能够结合生物物种,所述锚定基团能够结合至氧化还原聚合物。 然后将电极暴露于包含抗体 - 酶物质的抗体 - 酶液体,所述抗体 - 酶物质包含能够结合生物物种的检测抗体,所述检测抗体与氧化还原酶偶联,由此,如果分析物 液体包括生物物种,氧化还原酶通过检测抗体和结合抗体与生物物种的偶联而与电极偶联。 然后将电极暴露于包含氧化还原聚合物和酶底物的聚合物溶液,由此如果氧化还原酶与电极上的锚定基团偶联,则氧化还原聚合物被还原并连接到电极上的锚定基团。 然后将电压施加在电极和参考电极之间,并且电极暴露于可氧化物质,由此所述电极和参比电极之间的电流的大小表示生物物种的存在或不存在。