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21. 发明申请

WO2010142773A2 SEMICONDUCTOR BIOSENSORS 审中-公开
标题翻译：半导体生物传感器
公开(公告)号：WO2010142773A2
公开(公告)日：2010-12-16
申请号：PCT/EP2010/058183
申请日：2010-06-10
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) , THALHAMMER, Stefan , HOFSTETTER, Markus , HOWGATE, John , STUTZMANN, Martin
发明人： THALHAMMER, Stefan , HOFSTETTER, Markus , HOWGATE, John , STUTZMANN, Martin
IPC分类号： G01N27/414
CPC分类号： G01T1/02 , G01N33/48728
摘要： The present invention relates to semiconductor devices, in particular to a device for monitoring a cell signal such as an electrical signal produced by living cells in response to external stimulation, optionally in real time, comprising (a) at least one discrete area comprising a high electron mobility transistor (HEMT); and (b)non-excitable cells attached to said HEMT (HEMT element). Preferably, said device, and preferably said at least one discrete area which comprises a HEMT, has a surface suitable for cell attachment or growth. Accordingly, the device can be applied in uses and methods for monitoring a cell signal such as an electrical signal produced by living cells in response to external stimulation, optionally in real time. Likewise, the device can be applied for screening compounds that reverse, protect from and/or shield cells from external stimuli which cause damage to cells. Also, kits comprising the device are an aspect of the present invention.
摘要翻译：本发明涉及半导体器件，特别涉及一种用于监测细胞信号（例如，响应于外部刺激而由活细胞产生的电信号，可选地实时地）产生的电信号的装置，包括：（a）至少一个离散的区域，包括高的电子迁移率晶体管（HEMT）; 和（b）附着于所述HEMT（HEMT元件）的不可兴奋细胞。优选地，所述装置，优选地包括HEMT的所述至少一个离散区域具有适于细胞附着或生长的表面。因此，该设备可以应用于可选地实时监视响应于外部刺激的由活细胞产生的电信号的细胞信号的用途和方法。同样，该装置可用于筛选从外部刺激反向，保护和/或屏蔽细胞的化合物，从而引起细胞损伤。而且，包括该装置的试剂盒是本发明的一个方面。

22. 发明申请

WO2010012829A1 HER2/NEU SPECIFIC T CELL RECEPTORS 审中-公开
标题翻译： HER2 / NEU特异性细胞受体
公开(公告)号：WO2010012829A1
公开(公告)日：2010-02-04
申请号：PCT/EP2009/059953
申请日：2009-07-31
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) , KRACKHARDT, Angela , WEIGAND, Luise , LIANG, Xiaoling
发明人： KRACKHARDT, Angela , WEIGAND, Luise , LIANG, Xiaoling
IPC分类号： C07K14/705 , C12N5/10 , C12N15/63 , A61K48/00
CPC分类号： C07K14/7051
摘要： The present invention is directed to T cell receptors (TCR) recognizing antigenic peptides derived from Her2/neu, in particular peptide 369, and being capable of inducing peptide specific killing of a target cell overexpressing HER2/neu. The present invention is further directed to an antigen specific T cell, comprising said TCR, to a nucleic acid coding for said TCR and to the use of the antigen specific T cells for the manufacture of a medicament for the treatment of malignancies characterized by overexpression of HER2/neu. The present invention is further disclosing a method of generating antigen specific T cells.
摘要翻译：本发明涉及识别衍生自Her2 / neu，特别是肽369的抗原肽的T细胞受体（TCR），并能够诱导过度表达HER2 / neu的靶细胞的肽特异性杀伤。本发明还涉及包含所述TCR的抗原特异性T细胞与编码所述TCR的核酸以及使用抗原特异性T细胞用于制备用于治疗特征在于过表达的恶性肿瘤的药物 HER2 / neu基因。本发明还公开了产生抗原特异性T细胞的方法。

23. 发明申请

WO2009124777A1 CIS-ACTING DIVERSIFICATION ACTIVATOR AND METHOD FOR SELECTIVE DIVERSIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：多样化ACTIVATOR ACTING <我
公开(公告)号：WO2009124777A1
公开(公告)日：2009-10-15
申请号：PCT/EP2009/002680
申请日：2009-04-09
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) , BUERSTEDDE, Jean-Marie , CALDWELL, Randolph, B.
发明人： BUERSTEDDE, Jean-Marie , CALDWELL, Randolph, B.
IPC分类号： C12N15/00 , C12N15/01 , C12N5/06
CPC分类号： C12N15/1024
摘要： The invention relates to identification of a cis -acting diversification activator ( DIVAC ) that is necessary and sufficient for the activation of diversification in transcription units linked thereto. The invention provides a method for diversification of a target nucleic acid comprising introducing a genetic construct comprising the diversification activator into a recipient cell, wherein the diversification activator is linked to the target nucleic acid.
摘要翻译：公开了一种多样化活化剂的顺式作用（迪瓦茨）是必要的和足够的多样化在转录单位与其连接的激活的识别。本发明提供一种靶核酸，其包含导入的基因构建体，其包括多样化活化剂到受体细胞的多样化的方法，所述多样化活化剂连接到靶核酸。

24. 发明申请

WO2009103502A1 METHOD AND DEVICE FOR NEAR-FIELD DUAL-WAVE MODALITY IMAGING 审中-公开
标题翻译：近场双波模态成像的方法与装置
公开(公告)号：WO2009103502A1
公开(公告)日：2009-08-27
申请号：PCT/EP2009/001137
申请日：2009-02-18
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH). , RAZANSKY, Daniel , NTZIACHRISTOS, Vasilis
发明人： RAZANSKY, Daniel , NTZIACHRISTOS, Vasilis
IPC分类号： A61B5/05 , A61B8/00 , G01N21/17
CPC分类号： A61B8/08 , A61B5/0095 , A61B5/05 , A61B8/00 , A61B2503/40 , G01N2021/1787
摘要： A method of near-field imaging a region of interest in an object comprises the steps emitting an input of near-field electromagnetic energy from a near-field source device into the region of interest, detecting a mechanical wave response generated in the region of interest in response to the near-field electromagnetic energy input, and providing image data representing an image of the region of interest on the basis of the mechanical wave response. Furthermore, a device for near-field imaging the region of interest is described. The invention relies on near-field exposure of the object to electromagnetic sources of safe energies. The physical basis of the image formation is the thermoacoustic phenomenon, which utilizes radio-frequency (RF) energy deposition onto the object under investigation. Subsequently, the absorbed electromagnetic energy generates acoustic response, which can be detected and used for forming volumetric visualization (image) of the underlining tissue absorption properties.
摘要翻译：对物体中的感兴趣区域进行近场成像的方法包括发射从近场源装置进入感兴趣区域的近场电磁能的输入的步骤，检测在感兴趣区域中产生的机械波响应响应于近场电磁能量输入，并且基于机械波响应提供表示感兴趣区域的图像的图像数据。此外，描述了用于对感兴趣区域进行近场成像的装置。本发明依靠物体近场暴露于安全能量的电磁源。图像形成的物理基础是热声现象，其利用射频（RF）能量沉积到被调查物体上。随后，吸收的电磁能产生声响应，其可以被检测并用于形成下划线组织吸收特性的体积可视化（图像）。

25. 发明申请

WO2009077205A1 ELECTROPHORETIC ANALYSIS OF ISOLATED MITOCHONDRIA FOR THE DETECTION OF CELL OR TISSUE DAMAGE 审中-公开
标题翻译：用于检测细胞或组织损伤的隔离麻醉剂的电泳分析
公开(公告)号：WO2009077205A1
公开(公告)日：2009-06-25
申请号：PCT/EP2008/010915
申请日：2008-12-19
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) , ZISCHKA, Hans
发明人： ZISCHKA, Hans
IPC分类号： G01N33/50
CPC分类号： G01N33/5079
摘要： The present invention relates to a method for detecting mitochondrial damage in mammalian tissue or cells comprising: (a) isolating mitochondria from a sample obtained from a mammal and containing tissue or cells containing mitochondria; (b1 ) separating populations of the isolated mitochondria of step (a) by means of their electrophoretic mobility; and (c1) comparing the populations of the mitochondria obtained in step (b1) with those of a reference sample containing tissue or cells displaying a non-pathological phenotype, wherein a rise in the number of populations, a shift of the population(s) towards the anode and/or an increase in the heterogeneity of one or more populations separated in step (b1) as compared to those of the reference sample is indicative of mitochondrial damage; or (b2) analyzing the average surface charge of the isolated mitochondria of step (a); and (c2) comparing the average surface charge determined in step (b2) with that of a reference sample containing cells displaying a non-pathological phenotype, wherein a shift of the average surface charge of the mitochondria analyzed in step (b2) to the negative as compared to that of the mitochondria of the reference sample is indicative of mitochondrial damage; and (d) in the case that a shift of the population(s) towards the cathode in step (c1) or a shift of the average surface charge to the positive in step (c2) is detected or, confirming that mitochondrial damage is present. Furthermore, the present invention relates to a method of identifying an agent causing mitochondrial damage in mammalian cells and a method of identifying an agent capable of improving the condition of mammalian mitochondria.
摘要翻译：本发明涉及一种用于检测哺乳动物组织或细胞中线粒体损伤的方法，包括：（a）从获自哺乳动物并含有含有线粒体的组织或细胞的样品中分离线粒体; （b1）通过其电泳迁移率分离步骤（a）的分离的线粒体的群体; 和（c1）将步骤（b1）中获得的线粒体的群体与含有显示非病理表型的组织或细胞的参考样品的群体进行比较，其中群体数量的增加，与参考样品相比，在步骤（b1）中分离的一个或多个群体的阳性和/或异质性的增加指示线粒体损伤; 或（b2）分析步骤（a）的分离的线粒体的平均表面电荷; 和（c2）比较步骤（b2）中测定的平均表面电荷与含有显示非病理表型的细胞的参考样品的平均表面电荷的比较，其中步骤（b2）中分析的线粒体的平均表面电荷的偏移为负与参考样品的线粒体相比，表明线粒体损伤; 和（d）在步骤（c1）中人群朝向阴极的移动或步骤（c2）中的平均表面电荷移动到阳性的情况下，或者确认存在线粒体损伤。此外，本发明涉及鉴定在哺乳动物细胞中引起线粒体损伤的药剂的方法，以及鉴定能够改善哺乳动物线粒体状态的药剂的方法。

26. 发明申请

WO2009056327A2 VERFAHREN UND VORRICHTUNG FÜR DEN NACHWEIS VON MINDESTENS EINER ZIELSUBSTANZ 审中-公开
标题翻译：方法和设备至少一个目标物质的检测
公开(公告)号：WO2009056327A2
公开(公告)日：2009-05-07
申请号：PCT/EP2008/009199
申请日：2008-10-31
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT GMBH , ENGLMANN, Matthias , SCHMITT-KOPPLIN, Philippe , GEBEFÜGI, Istvan , FRISCH, Heinz , DIETZ, Wolfgang
发明人： ENGLMANN, Matthias , SCHMITT-KOPPLIN, Philippe , GEBEFÜGI, Istvan , FRISCH, Heinz , DIETZ, Wolfgang
IPC分类号： H01J49/16
CPC分类号： H01J49/162 , H01J49/164
摘要： Verfahren für den Nachweis von mindestens einer Zielsubstanz, umfassend eine Überführung von Molekülen mindestens einer der Zielsubstanzen in einen gasförmigen Zustand sowie einen spektrometrischen Nachweis der Moleküle. Die Aufgabe liegt darin, das Verfahren mit einem signifikant erhöhten Auflösungsvermögen in der Nachweisgrenze sowie in der Selektivität zu schaffen. Dies wird dadurch gelöst, dass die Überführung eine lösliche Vermischung, Aerosolbildung und Verdampfung mindestens einer der Zielsubstanzen mit einem Lösungsmittel umfasst, wobei die Moleküle in eine Gasphase integriert werden, sowie der spektrometrische Nachweis eine Ionisierung der Moleküle in der Gasphase zu Ionen umfasst.
摘要翻译：一种用于检测至少一种目标物质的方法，包括在气体状态下的转印目标物质中的至少一个的分子的和分子的光谱检测。的目的是提供一种在检测限和在选择性的显著增加的分辨率的方法。这得以实现，所述传递包括用溶剂的目标物质中的至少一种，其中所述分子被整合到气相的可溶混合物，气溶胶的形成和挥发，且光谱检测包括在所述气相离子的分子的电离。

27. 发明申请

WO2023079127A1 MODULATORS OF MESOTHELIAL ECM MOVEMENT 审中-公开
公开(公告)号：WO2023079127A1
公开(公告)日：2023-05-11
申请号：PCT/EP2022/080892
申请日：2022-11-07
申请人： HELMHOLTZ ZENTRUM MÜNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH)
发明人： FISCHER, Adrian , MÜCK-HÄUSL, Martin , RINKEVICH, Yuval
IPC分类号： C07K14/495 , C12N15/86
摘要： The present invention relates to a compound for use in a method for the modulation of movement of extracellular matrix (ECM) produced by mesothelial cells forming the surface of an internal organ, towards a site of injury of said organ of a subject suffering from or being at a risk of an injury of said organ. Additionally, the present invention relates to a compound for use in a particular in vivo screening method for identifying a modulator of movement of extracellular matrix (ECM) produced by mesothelial cells towards a site of injury of an internal organ of a subject. Further, the present invention relates to a specific in vitro screening method for identifying a modulator of the movement of ECM towards an external stimulus in a single cell suspension derived from the mesothelium.

28. 发明申请

WO2023002024A1 DEVICE AND METHOD FOR DETERMINING PARAMETERS REGARDING A CONTENT OR PROPERTY OF BIOMOLECULES IN BIOLOGICAL TISSUES 审中-公开
公开(公告)号：WO2023002024A1
公开(公告)日：2023-01-26
申请号：PCT/EP2022/070644
申请日：2022-07-22
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH)
发明人： PLEITEZ RAFAEL, Miguel Angel , NTZIACHRISTOS, Vasilis
IPC分类号： A61B5/00 , A61B5/145 , G01N21/17 , G01N29/036 , G01N29/06
摘要： The invention relates to a device and a method for determining one or more parameters regarding a content and/or property of biomolecules, in particular metabolites, in a biological tissue (11). The device comprises at least one irradiation unit (1) configured to irradiate the tissue (11) with electromagnetic radiation (2) exhibiting one or more wavelengths or wavelength ranges, in particular in the mid-infrared, near-infrared and/or visible spectral range, at least one detection unit (8, 12) configured to detect, in a spatially resolved manner, first signals corresponding to optical and/or optoacoustic and/or optothermal signals emanating from the tissue (11) in response to irradiating the tissue (11) with electromagnetic radiation (2), in particular in the near-infrared and/or visible spectral range, wherein the at least one irradiation unit (1) and the at least one detection unit (8, 12) are located in the same half space above and/or adjacent to the irradiated tissue (11), and a processing unit (14) configured i) to localize, based on the detected first signals, a location or region of interest on and/or within the tissue (11), at which at least one biomolecule of interest is present or expected, ii) to control the at least one irradiation unit (1) to irradiate the location or region of interest on and/or within the tissue (11) with electromagnetic radiation (2) exhibiting a time-varying intensity and one or more wavelengths or wavelength ranges in the mid-infrared spectral range, iii) to control the at least one detection unit (8, 12) to detect second signals corresponding to optoacoustic and/or optothermal signals emanating from the irradiated location or region of interest on and/or within the tissue (11) in response to irradiating the location or region of interest on and/or within the tissue (11) with the electromagnetic radiation (2) in the mid-infrared spectral range, and iv) to determine, based on the detected second signals, one or more parameters regarding a content and/or property of the at least one biomolecule, in particular metabolite, of interest at the location or region of interest on and/or within in the tissue (11).

29. 发明申请

WO2022002773A1 OTOSCOPE 审中-公开
公开(公告)号：WO2022002773A1
公开(公告)日：2022-01-06
申请号：PCT/EP2021/067455
申请日：2021-06-25
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH)
发明人： BISCHOF, Thomas
IPC分类号： A61B1/227 , A61B1/00 , A61B1/06 , A61B1/07 , A61B1/00032 , A61B1/0669
摘要： The invention relates to an otoscope (100), comprising a light source (108) configured to emit light to illuminate an ear canal, visualization means (106) configured to display the ear canal illuminated by the light emitted by the light source (108) for visual inspection of the ear canal by a user, a spectral sensor (110) configured to measure intensities of the light emitted by the light source (108) and reflected by the ear canal, the spectral sensor (110) being configured to measure at least a first intensity in a first spectral range and a second intensity in a second spectral range, and a controller (112) configured to calculate a parameter on the basis of the first intensity and the second intensity.

30. 发明申请

WO2020120495A1 IN-VITRO METHOD FOR DIAGNOSIS AND PROGNOSIS OF A DISEASE 审中-公开
公开(公告)号：WO2020120495A1
公开(公告)日：2020-06-18
申请号：PCT/EP2019/084475
申请日：2019-12-10
申请人： HELMHOLTZ ZENTRUM MÜNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH)
发明人： WALCH, Axel , HUBER, Katharina , BUCK, Achim , KUNZKE, Thomas
IPC分类号： G01N33/68 , C12Q1/6813
摘要： The present invention relates an in-vitro method for diagnosis and/or prognosis of a disease in a tissue sample obtained from a mammalian subject comprising (i) Determining the level of a first biomarker using matrix-assisted laser desorption/ionization (MALDI)-imaging in the sample; (ii) Performing a histochemical staining of the sample; (iii) Determining the ratio of the levels of a second biomarker and a reference marker using fluorescence in situ -hybridization (FISH); wherein the same sample is used in steps (i) to (iii).

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