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21. 发明申请

WO2004013607A2 METHODS, DEVICES AND KITS FOR MULTIPLEX BLOTTING OF BIOLOGICAL SAMPLES FROM MULTI-WELL PLATES 审中-公开
标题翻译：用于多孔板生物样品多重喷涂的方法，装置和套件
公开(公告)号：WO2004013607A2
公开(公告)日：2004-02-12
申请号：PCT/US2003/024225
申请日：2003-08-01
申请人： 20/20 GENESYSTEMS, INC. , THE GOVERNMENT OF THE UNITED STATES AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES , KNEZEVIC, Vladimir , HARTMANN, Dan-Paul , EMMERT-BUCK, Michael, R.
发明人： KNEZEVIC, Vladimir , HARTMANN, Dan-Paul , EMMERT-BUCK, Michael, R.
IPC分类号： G01N
CPC分类号： B01L3/50255 , B01J19/0046 , B01J2219/00416 , B01J2219/00533 , B01J2219/00574 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00668 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01L2300/0627 , B01L2300/0829 , B01L2400/049 , C40B40/06 , C40B40/10 , G01N33/54373
摘要： The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
摘要翻译：本公开提供了允许在源自多样品保持器（例如多孔板）的样品中同时检测大量目标生物分子的方法，装置和试剂盒。一个具体示例性方法是制造多孔样品架的生物分子含量的多个实质复制品的方法。还提供了执行所述方法的设备和套件。

22. 发明申请

WO2003090780A1 DIRECT CELL TARGET ANALYSIS 审中-公开
标题翻译：直接细胞目标分析
公开(公告)号：WO2003090780A1
公开(公告)日：2003-11-06
申请号：PCT/US2003/012734
申请日：2003-04-25
申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES , EMMERT-BUCK, Michael, R. , AHRAM, Mamoun
发明人： EMMERT-BUCK, Michael, R. , AHRAM, Mamoun
IPC分类号： A61K39/395
CPC分类号： H04B10/0731 , G01N33/5091 , G01N33/567 , G01N33/6857 , G01N2333/908 , H04B10/2916
摘要： Disclosed herein are Direct Cell Target Analysis ("DCTA") molecules and Direct Cell Target ("DCT") methods for directly targeting and acting upon biomolecules. These methods and molecules can be used with specific cells within complex, heterogeneous tissue such that target biomolecules can be procured for subsequent analysis or directly analyzed without the need for physical separation of the biomolecules from other cells or cell components in the population. In general, the methods involve use of a fusion molecule having a first moiety to identify and localize target cells within a tissue sample, and a second moiety to generate detectable products within the target cells that may be detected and subsequently analyzed, and optionally isolated.
摘要翻译：本文公开了用于直接靶向和作用于生物分子的直接细胞靶分析（“DCTA”）分子和直接细胞靶（“DCT”）方法。这些方法和分子可以与复杂的异质组织中的特定细胞一起使用，使得靶生物分子可以被采购用于随后的分析或直接分析，而不需要生物分子与群体中其他细胞或细胞组分的物理分离。通常，所述方法包括使用具有第一部分的融合分子来鉴定和定位组织样品中的靶细胞，以及第二部分以在靶细胞内产生可被检测和随后分析并任选分离的可检测产物。

23. 发明申请

WO2002048674A3 METHODS, DEVICES, ARRAYS AND KITS FOR DETECTING AND ANALYZING BIOMOLECULES 审中-公开
公开(公告)号：WO2002048674A3
公开(公告)日：2002-06-20
申请号：PCT/US2001/044009
申请日：2001-11-20
申请人： 20/20 GENE SYSTEMS, INC. , THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH & HUMAN SERVICES, THE NATIONAL INSTITUTES OF HEALTH , KNEZEVIC, Vladimir , EMMERT-BUCK, Michael, R. , BAIBAKOVA, Galina , HARTMANN, Dan-Paul , HEWITT, Stephen , MITCHELL, Capre, Denise , GARDNER, Kevin
发明人： KNEZEVIC, Vladimir , EMMERT-BUCK, Michael, R. , BAIBAKOVA, Galina , HARTMANN, Dan-Paul , HEWITT, Stephen , MITCHELL, Capre, Denise , GARDNER, Kevin
IPC分类号： C12M1/34
摘要： The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section or other substantially two-dimensional sample (e.g., electrophoretic gel or cDNA microarray) by creating "carbon copies" of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules in the samples. Other specific methods are provided for the identification and analysis of biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The disclosure also provides a plurality of differentially prepared and/or processed membranes for use with the described methods, and which permit identification and analysis of biomolecules. For example, Figure 1 shows a menbrane array (13) in which molecules are transferred from a tissue section (11) using a wick (20).

24. 发明申请

WO0210751A2 TRANSFER MICRODISSECTION 审中-公开
标题翻译：转移显微镜
公开(公告)号：WO0210751A2
公开(公告)日：2002-02-07
申请号：PCT/US0108095
申请日：2001-03-14
申请人： US GOV HEALTH & HUMAN SERV , EMMERT BUCK MICHAEL R , ENGLERT CHAD R , BONNER ROBERT F , LIOTTA LANCE A
发明人： EMMERT-BUCK MICHAEL R , ENGLERT CHAD R , BONNER ROBERT F , LIOTTA LANCE A
IPC分类号： G01N1/28 , G01N33/50 , G01N33/543
CPC分类号： G01N33/5005 , G01N1/2813 , G01N33/54386 , G01N2001/284
摘要： The present disclosure concerns methods, systems, and devices for analyzing a biological material, such as a cellular or other specimen. In one disclosed example, the method selectively transfers biomolecules from a target region of interest in a biological sample (such as a tissue section). The transfer may occur, for example, by selectively focally altering a characteristic of a transfer layer adjacent the target region, such that the biomolecules can move through the altered area of the transfer layer. In particular examples, the transfer layer is altered by focally increasing a permeability of the transfer layer, for example by removing a focal portion of the transfer layer, and transporting the biomolecules through the altered region of the transfer layer, to microdissect the biomolecules of interest from the biological sample. In yet other embodiments, the microdissected biomolecules can be applied to an analysis substrate containing an identification molecule, such as a nucleic acid array, layered expression scan, or wells containing antibodies. Transfer microdissection allows biomolecules from regions of interest in the biological specimen to be selectively analyzed. For example, nests of highly atypical or metastatic cells in a tumor section can be analyzed for differential expression of certain proteins.
摘要翻译：本公开涉及用于分析生物材料（例如细胞或其它样品）的方法，系统和装置。在一个公开的实施例中，该方法选择性地将生物分子从目标感兴趣区域转移到生物样品（例如组织切片）中。转移可以例如通过选择性地改变邻近目标区域的转移层的特性，使得生物分子可以移动通过转移层的改变的区域而发生。在具体实例中，转移层通过聚焦增加转移层的渗透性而被改变，例如通过去除转移层的焦点部分，以及将生物分子传输通过转移层的改变区域，以显微分解感兴趣的生物分子来自生物样品。在其他实施方案中，显微切割的生物分子可以应用于含有鉴定分子，如核酸阵列，分层表达扫描或含有抗体的孔的分析底物。转移显微切割允许选择性分析来自生物样本中感兴趣区域的生物分子。例如，可以分析肿瘤部分中的非典型或转移性细胞的巢，以鉴别某些蛋白质的差异表达。

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