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11. 发明申请

WO2005023091A3 METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS 审中-公开
标题翻译：无创性早期诊断方法
公开(公告)号：WO2005023091A3
公开(公告)日：2005-06-16
申请号：PCT/US2004028857
申请日：2004-09-07
申请人： UNIV BOSTON , UNIV HONG KONG CHINESE , CANTOR CHARLES R , DING CHUNMING , LO YUK MING DENNIS , CHIU ROSSA WAI-KWUN
发明人： CANTOR CHARLES R , DING CHUNMING , LO YUK MING DENNIS , CHIU ROSSA WAI-KWUN
IPC分类号： A61B20060101 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6872 , C12Q2600/156 , C12Q2600/172 , C12Q2535/125
摘要： The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in biological sample.
摘要翻译：本发明涉及检测生物样品中的核酸的方法。该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。该方法可以用于例如诊断，预后和治疗目的。该方法可以用于例如诊断，预后和治疗目的。该方法允许准确检测生物样品中存在的非常少量的核酸。例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和移植受体中的供体特异性酸。在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其他外源核酸。

12. 发明申请

WO2004022721A3 QUANTIFICATION OF GENE EXPRESSION 审中-公开
标题翻译：量化基因表达
公开(公告)号：WO2004022721A3
公开(公告)日：2004-09-16
申请号：PCT/US0328081
申请日：2003-09-05
申请人： UNIV BOSTON , CANTOR CHARLES R , DING CHUNMING
发明人： CANTOR CHARLES R , DING CHUNMING
IPC分类号： G01N33/50 , C12N20060101 , C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2545/107 , C12Q2535/125 , C12Q2525/186
摘要： The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a "target nucleic acid sequence." Use of such standard in combination with a method of "enhancing" the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the "enhanced" standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.
摘要翻译：本发明涉及使用被设计为与目标基因或“目标核酸序列”相比具有一个碱基差异的标准品来测量样品中目标核酸的量的方法。使用这样的标准与“增强”标准和测试核酸样品中差异的方法结合使用，例如，在突变位点正确携带的碱基延伸反应，允许标准和靶核酸与相同的效率并且便于靶核酸的定量。此后，使用量化“增强的”标准和靶核酸样品的手段来确定靶核酸的量。在优选实施例中，量化装置是质谱仪。

13. 发明申请

WO2006058226A3 MODIFIED DIMERIC STREPTAVIDINS AND USES THEREOF 审中-公开
公开(公告)号：WO2006058226A3
公开(公告)日：2006-08-24
申请号：PCT/US2005042766
申请日：2005-11-23
申请人： UNIV BOSTON , CANTOR CHARLES R , ASLAN FILIZ M
发明人： CANTOR CHARLES R , ASLAN FILIZ M
IPC分类号： C07K14/36 , C12N15/10 , C12N15/12 , C12N15/62 , C40B40/10
CPC分类号： C12N15/1044 , C07K14/36
摘要： The present invention provides novel modified streptavidin proteins, nucleic acids encoding such proteins, and uses of such proteins. Specifically, the invention provides single-chain dimeric streptavidin molecules. The invention also provides modified single-chain streptavidins with altered affinity to biotin and biotin derivatives, such as biotin-4-fluorescein. The modified single-chain dimeric streptavidin proteins can be used in analysis or composite separation either alone or in combination with ordinary streptavidin-biotin systems to visualize and/or separate composites and molecules. The single-chain streptavidin dimers of the invention also allow creating of more stable streptavidin tetramers. The single-chain streptavidin dimers of the invention can further be used as smaller sized functional streptavidin molecules in various in vivo methods, where the size of the conventional tetrameric streptavidin makes the use of such tetramers difficult or impossible. Such in vivo uses include diagnostic imaging and targeting of therapeutic molecules into individuals. The invention further provides methods of preparing and identifying novel modified single-chain streptavidin molecules.

14. 发明申请

WO2006058226A9 MODIFIED DIMERIC STREPTAVIDINS AND USES THEREOF 审中-公开
标题翻译：改性的二甲基甲酰四环素及其用途
公开(公告)号：WO2006058226A9
公开(公告)日：2006-07-13
申请号：PCT/US2005042766
申请日：2005-11-23
申请人： UNIV BOSTON , CANTOR CHARLES R , ASLAN FILIZ M
发明人： CANTOR CHARLES R , ASLAN FILIZ M
IPC分类号： C07K14/36 , C12N15/10 , C12N15/12 , C12N15/62 , C40B40/10
CPC分类号： C12N15/1044 , C07K14/36
摘要： The present invention provides novel modified streptavidin proteins, nucleic acids encoding such proteins, and uses of such proteins. Specifically, the invention provides single-chain dimeric streptavidin molecules. The invention also provides modified single-chain streptavidins with altered affinity to biotin and biotin derivatives, such as biotin-4-fluorescein. The modified single-chain dimeric streptavidin proteins can be used in analysis or composite separation either alone or in combination with ordinary streptavidin-biotin systems to visualize and/or separate composites and molecules. The single-chain streptavidin dimers of the invention also allow creating of more stable streptavidin tetramers. The single-chain streptavidin dimers of the invention can further be used as smaller sized functional streptavidin molecules in various in vivo methods, where the size of the conventional tetrameric streptavidin makes the use of such tetramers difficult or impossible. Such in vivo uses include diagnostic imaging and targeting of therapeutic molecules into individuals. The invention further provides methods of preparing and identifying novel modified single-chain streptavidin molecules.
摘要翻译：本发明提供了新的修饰的链霉抗生物素蛋白，编码这些蛋白的核酸，以及这些蛋白的用途。具体而言，本发明提供了单链二聚体链霉抗生物素蛋白分子。本发明还提供了对生物素和生物素衍生物如生物素-4-荧光素具有改变的亲和性的修饰的单链抗生物素蛋白链菌素。修饰的单链二聚抗生蛋白链菌素蛋白可以单独用于分析或复合分离，或与普通链霉抗生物素蛋白 - 生物素系统组合使用，以显现和/或分离复合物和分子。本发明的单链抗生蛋白链菌素二聚体还允许产生更稳定的抗生蛋白链菌素四聚体。本发明的单链抗生蛋白链菌素二聚体可以进一步在各种体内方法中用作较小尺寸的功能性抗生蛋白链菌素分子，其中常规四聚链霉抗生物素蛋白的大小使得使用这种四聚体困难或不可能。这种体内用途包括诊断成像和将治疗分子靶向至个体。本发明进一步提供了制备和鉴定新型修饰的单链抗生蛋白链菌素分子的方法。

15. 发明申请

WO2004033485A3 NUCLEIC ACID SUPPORTED PROTEIN COMPLEMENTATION 审中-公开
标题翻译：核酸支持蛋白质补充
公开(公告)号：WO2004033485A3
公开(公告)日：2004-07-22
申请号：PCT/US0332426
申请日：2003-10-09
申请人： UNIV BOSTON , CANTOR CHARLES R , BROUDE NATALIA E , WITTE-HOFFMANN CARLOS
发明人： CANTOR CHARLES R , BROUDE NATALIA E , WITTE-HOFFMANN CARLOS
IPC分类号： C07H21/04 , C07K20060101 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6813 , C12Q2563/131 , C12Q2561/107
摘要： The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.
摘要翻译：本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。本发明基于蛋白质互补，其中两个单独的多肽是无活性的。当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。

16. 发明申请

WO0196530A8 DIMERIC STREPTAVIDINS 审中-公开
标题翻译：二维STREPTAVIDINS
公开(公告)号：WO0196530A8
公开(公告)日：2002-03-28
申请号：PCT/US0141027
申请日：2001-06-18
申请人： UNIV BOSTON , CANTOR CHARLES R , ASLAN FILIZ M , VAJDA SANDOR , SANO TAKESHI
发明人： CANTOR CHARLES R , ASLAN FILIZ M , VAJDA SANDOR , SANO TAKESHI
IPC分类号： C07K14/36 , C07K14/00
CPC分类号： C07K14/36
摘要： L'invention concerne des protéines de streptavidine recombinantes se liant à la biotine, des protéines de streptavidine recombinantes présentant une affinité altérée pour se lier à la biotine, ainsi que des méthodes d'utilisation de protéines de streptavidine recombinantes en vue de la détection et de l'isolation de cibles. L'invention se rapporte également à des acides nucléiques codant pour ces protéines de streptavidine recombinantes, ainsi qu'à des cellules recombinantes contenant et exprimant les protéines codées par ces acides nucléiques.

17. 发明申请

WO0196607A2 USE OF NUCLEOTIDE ANALOGS IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN HIGHLY MULTIPLEXED NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：核苷类似物在寡核苷酸混合物和高度多核苷酸序列分析中的应用
公开(公告)号：WO0196607A2
公开(公告)日：2001-12-20
申请号：PCT/US0119249
申请日：2001-06-13
申请人： UNIV BOSTON , CANTOR CHARLES R , SIDDIQI FOUAD A
发明人： CANTOR CHARLES R , SIDDIQI FOUAD A
IPC分类号： C12Q1/68 , G06F19/22
CPC分类号： G06F19/22 , C12Q1/6872 , H01J49/00 , C12Q2563/167 , C12Q2525/301 , C12Q2521/307
摘要： Methods and kits that use nucleotide analogs to confer increased accuracy and improved resolution in the analysis and sequencing of oligonucleotide mixtures are provided.
摘要翻译：提供了使用核苷酸类似物在寡核苷酸混合物的分析和测序中提供更高准确度和改进的分辨率的方法和试剂盒。

18. 发明申请

WO2013052907A8 METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS 审中-公开
标题翻译：用于遗传变异的非侵入性评估的方法和过程
公开(公告)号：WO2013052907A8
公开(公告)日：2013-09-19
申请号：PCT/US2012059114
申请日：2012-10-05
申请人： SEQUENOM INC
发明人： VAN DEN BOOM DIRK JOHANNES , CANTOR CHARLES R , KIM SUNG KYUN , DZAKULA ZELJKO , DECIU COSMIN
IPC分类号： G06F19/00
CPC分类号： C12Q1/6869 , C12Q1/6827 , G06F19/18 , G06F19/22 , G06F19/24 , C12Q2537/16 , C12Q2537/165
摘要： Technology provided herein relates in part to methods, processes and apparatuses for non-invasive assessment of genetic variations.
摘要翻译：本文提供的技术部分涉及用于遗传变异的非侵入性评估的方法，过程和装置。

19. 发明申请

WO2013052907A3 METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS 审中-公开
公开(公告)号：WO2013052907A3
公开(公告)日：2013-10-17
申请号：PCT/US2012059114
申请日：2012-10-05
申请人： SEQUENOM INC
发明人： VAN DEN BOOM DIRK JOHANNES , CANTOR CHARLES R , KIM SUNG KYUN , DZAKULA ZELJKO , DECIU COSMIN
IPC分类号： G06F19/18 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6827 , G06F19/18 , G06F19/22 , G06F19/24 , C12Q2537/16 , C12Q2537/165
摘要： Technology provided herein relates in part to methods, processes and apparatuses for non-invasive assessment of genetic variations.

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