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    • 14. 发明申请
    • METHOD FOR ASSAYING ANTIGENS
    • 测定抗原的方法
    • WO2007122373A8
    • 2009-07-16
    • PCT/GB2007001353
    • 2007-04-12
    • AVECIA BIOLOG LTDWATKINSON ALLANWILSON ROBERTDOUGLASS STEVENMYERS JAMES PHILIP
    • WATKINSON ALLANWILSON ROBERTDOUGLASS STEVENMYERS JAMES PHILIP
    • G01N33/553G01N33/543
    • G01N33/56911G01N2333/32
    • A method for assaying an antigen adsorbed on a solid support is provided. The method comprises: a) contacting a suspension of the antigen adsorbed on the solid support with a solution or suspension of an optionally detectably-labelled primary antibody to the antigen to form a solid-supported antigen-antibody complex; b) where the primary antibody is detectably labelled, optionally measuring the detectable label thereby to determine the quantity of the antigen; or c) contacting the solid-supported antigen-antibody complex with a detectably-labelled probe for the solid-supported antigen-antibody complex; and d) measuring the detectable label on the probe, thereby to determine the quantity of antigen. The antigen is preferably a component of a sub-unit vaccine, and most preferably anthrax protective antigen.
    • 提供了用于测定吸附在固体支持物上的抗原的方法。 该方法包括:a)将吸附在固体支持物上的抗原悬浮液与任选可检测标记的一抗的溶液或悬浮液接触,以形成固体支持的抗原 - 抗体复合物; b)其中一抗被可检测地标记,任选地测量可检测标记,从而确定抗原的量; 或c)使固体支持的抗原 - 抗体复合物与固体支持的抗原 - 抗体复合物的可检测标记的探针接触; 和d)测量探针上的可检测标记,从而确定抗原的量。 抗原优选为亚单位疫苗的组分,最优选为炭疽保护性抗原。