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    • 14. 发明申请
    • METHOD FOR POLYNUCLEOTIDE AMPLIFICATION
    • 多核苷酸扩增方法
    • WO1998028443A1
    • 1998-07-02
    • PCT/US1997023706
    • 1997-12-17
    • DADE BEHRING MARBURG GMBHULLMAN, Edwin, F.
    • DADE BEHRING MARBURG GMBHLISHANSKI, AllaKURN, Nurith
    • C12Q01/68
    • C12Q1/6848C12Q2525/186C12Q2525/161C12Q2521/325
    • The present invention relates to a method for selectively extending an oligonucleotide primer along a specific target polynucleotide sequence in a mixture of polynucleotides. The method comprises providing the modified oligonucleotide having a 3'-end that is not extendable along any polynucleotide and extending the oligonucleotide primer selectively along the specific target polynucleotide sequence by controlling the degradation of the 3'-end of the modified oligonucleotide. In this way extension along polynucleotides other than the specific target polynucleotide sequence is substantially reduced or avoided. In another aspect the invention is an improvement in a method for amplifying a target polynucleotide sequence. The improvement comprises deriving the oligonucleotide primer from a modified oligonucleotide having a portion that hybridizes to the target polynucleotide sequence except for the 3'-end thereof, which has at least one nucleotide analog that is incapable of hybridizing to a polyuncleotide. Kits for carrying out the above methods are also disclosed.
    • 本发明涉及在多核苷酸的混合物中选择性延伸寡核苷酸引物沿着特异性目标多核苷酸序列的方法。 该方法包括提供具有3'端的修饰寡核苷酸,其不能沿着任何多核苷酸延伸,并通过控制修饰寡核苷酸的3'末端的降解,沿特异性靶多核苷酸序列选择性延伸寡核苷酸引物。 以这种方式,除特异性靶多核苷酸序列以外的多核苷酸的延伸被显着降低或避免。 在另一方面,本发明是扩增靶多核苷酸序列的方法的改进。 该改进包括从具有至少一个不能与多核苷酸杂交的核苷酸类似物的除3'-末端以外的靶多核苷酸序列杂交的部分修饰的寡核苷酸导出寡核苷酸引物。 还公开了用于实施上述方法的套件。