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11. 发明申请

WO2010085289A1 NOVEL BACILLUS THURINGIENSIS GENE WITH LEPIDOPTERAN ACTIVITY 审中-公开
标题翻译：新型苏云金芽孢杆菌基因与鳞翅目动物
公开(公告)号：WO2010085289A1
公开(公告)日：2010-07-29
申请号：PCT/US2009/064000
申请日：2009-11-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ABAD, Andre, R. , DONG, Hua , LO, Sue, B. , SHI, Xiaomei
发明人： ABAD, Andre, R. , DONG, Hua , LO, Sue, B. , SHI, Xiaomei
IPC分类号： C12N15/31 , C07K14/325 , A01N63/02 , C12N1/20 , C12R1/07
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera . Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供了从编码具有抗虫害杀虫活性的多肽的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，所述多肽对包括鳞翅目昆虫 >。本发明的具体实施方案提供了编码杀虫蛋白的分离的核酸，杀虫组合物，DNA构建体和包含实施方案的核酸的转化的微生物和植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

12. 发明申请

WO2009088735A2 NOVEL BACILLUS THURINGIENSIS GENE WITH COLEOPTERAN ACTIVITY 审中-公开
公开(公告)号：WO2009088735A2
公开(公告)日：2009-07-16
申请号：PCT/US2008/087946
申请日：2008-12-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ABAD, Andre, R. , DONG, Hua , LO, Sue, B. , SHI, Xiaomei
发明人： ABAD, Andre, R. , DONG, Hua , LO, Sue, B. , SHI, Xiaomei
IPC分类号： C12N15/82
CPC分类号： C07K14/32 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera . Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

13. 发明申请

WO2008011565A2 METHOD FOR IDENTIFYING NOVEL GENES 审中-公开
标题翻译：鉴定新基因的方法
公开(公告)号：WO2008011565A2
公开(公告)日：2008-01-24
申请号：PCT/US2007073983
申请日：2007-07-20
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , DONG HUA , LO SUSAN B , MCCUTCHEN BILLY F , SHI XIAOMEI
发明人： ABAD ANDRE R , DONG HUA , LO SUSAN B , MCCUTCHEN BILLY F , SHI XIAOMEI
IPC分类号： C12Q1/68
CPC分类号： C12N15/8286 , C12Q1/6813 , Y02A40/162 , C12Q2531/113
摘要： Methods and compositions for identifying novel genes that share regions of homology with known genes from target groups of genes of interest are provided. The methods comprise systematically designing oligonucleotide primers that are specific for regions of homology within the nucleotide sequences of a target group of known genes and performing successive rounds of PCR amplification of nucleic acid material from an organism of interest. The PCR steps are intended to identify and amplify nucleic acids comprising both known and novel genes. Nucleic acid molecules comprising known genes are detected and eliminated from further consideration by dot blot analysis using oligonucleotide probes specific for the known genes in the target group. Potentially novel genes are subjected to further sequence analysis to confirm novelty and assayed for biological activity. Compositions of the present invention include novel polynucleotides, and variants and fragments thereof, that comprise novel genes and the polypeptides encoded thereby.
摘要翻译：提供了用于鉴定与来自目标基因目标组的已知基因具有同源性区域的新基因的方法和组合物。所述方法包括系统地设计对已知基因的目标组的核苷酸序列内的同源性区域特异的寡核苷酸引物，并且从感兴趣的生物进行核酸材料的连续循环PCR扩增。 PCR步骤旨在鉴定和扩增包含已知和新型基因的核酸。包含已知基因的核酸分子通过使用对靶基因中已知基因具有特异性的寡核苷酸探针的斑点印迹分析进一步考虑而被检测和消除。对潜在的新基因进行进一步的序列分析以确认新颖性并测定生物活性。本发明的组合物包括新的多核苷酸及其变体和片段，其包含新基因和由此编码的多肽。

14. 发明申请

WO2006107954A3 METHODS AND COMPOSITIONS FOR DESIGNING NUCLEIC ACID MOLECULES FOR POLYPEPTIDE EXPRESSION IN PLANTS USING PLANT VIRUS CODON-BIAS 审中-公开
标题翻译：使用植物病毒CODON-BIAS设计植物中多肽表达的核酸分子的方法和组合物
公开(公告)号：WO2006107954A3
公开(公告)日：2007-02-22
申请号：PCT/US2006012478
申请日：2006-04-04
申请人： PIONEER HI BRED INT , DU PONT , SIMMONS CARL R , ABAD ANDRE R , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F
发明人： ABAD ANDRE R , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F
IPC分类号： C12N15/82 , A01H5/00 , C12N15/67
CPC分类号： C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.
摘要翻译：本发明涉及设计核酸分子以改进植物中编码多肽表达的方法。在这种方法中，密码子使用频率偏向于植物病毒，植物病毒组或其核酸分子的子集的密码子使用频率。在优选的实施方案中，编码的多肽影响植物的表型。本发明还涉及编码杀虫多肽的核酸分子，其中核酸分子被设计为具有植物病毒密码子偏倚。本发明还涉及具有增加的杀虫多肽表达的转基因植物及其后代，以改善对对农业价值的植物有害的昆虫和其它害虫的抗性。

15. 发明申请

WO2012151145A1 BACTERIAL MRNA SCREEN STRATEGY FOR NOVEL PESTICIDE-ENCODING NUCLEIC ACID MOLECULE DISCOVERY 审中-公开
标题翻译：新型杀虫剂编码核酸分子发现的细菌MRNA筛选策略
公开(公告)号：WO2012151145A1
公开(公告)日：2012-11-08
申请号：PCT/US2012/035740
申请日：2012-04-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ABAD, Andre, R. , KAPKA-KITZMAN, Deirdre, M.
发明人： ABAD, Andre, R. , KAPKA-KITZMAN, Deirdre, M.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C12N15/1072 , C12N15/8279 , Y02A40/162
摘要： Methods are provided for isolating and identifying novel pesticide-encoding nucleic acid molecules from bacterial strains identified as having a pesticidal polypeptide. The methods involve selecting a bacterial strain having or suspected of having at least one pesticide-encoding plasmid, curing the at least one pesticide-encoding plasmid from the bacterial strain and performing a subtractive hybridization to obtain plasmid mRNA encoding the pesticidal polypeptide. The plasmid mRNA can be used to make a cDNA library from which the pesticidal polypeptide can be isolated and subsequently identified.
摘要翻译：提供了从被鉴定为具有杀虫多肽的细菌菌株中分离和鉴定新型农药编码核酸分子的方法。所述方法包括选择具有或怀疑具有至少一种农药编码质粒的细菌菌株，从细菌菌株固化至少一种农药编码质粒，并进行消减杂交以获得编码杀虫多肽的质粒mRNA。质粒mRNA可用于制备可从其中分离并随后鉴定的杀虫多肽的cDNA文库。

16. 发明申请

WO2012024176A3 NOVEL BACILLUS THURINGIENSIS GENE WITH LEPIDOPTERAN ACTIVITY 审中-公开
公开(公告)号：WO2012024176A3
公开(公告)日：2012-05-10
申请号：PCT/US2011047536
申请日：2011-08-12
申请人： PIONEER HI BRED INT , ABAD ANDRE R , DONG HUA , LO SUE B , SHI XIAOMEI , WOLFE THOMAS C
发明人： ABAD ANDRE R , DONG HUA , LO SUE B , SHI XIAOMEI , WOLFE THOMAS C
IPC分类号： C12N15/31 , A01H5/00 , A01N63/02 , C07K14/32 , C12N15/82
CPC分类号： C07K14/32 , A01N63/02 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

17. 发明申请

WO2011084314A3 NOVEL BACILLUS THURINGIENSIS GENE WITH COLEOPTERAN ACTIVITY 审中-公开
公开(公告)号：WO2011084314A3
公开(公告)日：2011-10-27
申请号：PCT/US2010059378
申请日：2010-12-08
申请人： PIONEER HI BRED INT , ABAD ANDRE R , DONG HUA , LO SUE B , SHI XIAOMEI , WOLFE THOMAS C
发明人： ABAD ANDRE R , DONG HUA , LO SUE B , SHI XIAOMEI , WOLFE THOMAS C
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82 , C12R1/07
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

18. 发明申请

WO2011087642A2 NOVEL BACILLUS THURINGIENSIS GENE WITH COLEOPTERAN ACTIVITY 审中-公开
标题翻译：新颖的BACILLUS THURINGIENSIS基因与COOPOPTERAN活动
公开(公告)号：WO2011087642A2
公开(公告)日：2011-07-21
申请号：PCT/US2010/059609
申请日：2010-12-09
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ABAD, Andre R. , DONG, Hua , LO, Sue B. , SHI, Xiaomei
发明人： ABAD, Andre R. , DONG, Hua , LO, Sue B. , SHI, Xiaomei
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera . Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鞘翅目。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

19. 发明申请

WO2011084324A2 NOVEL BACILLUS THURINGIENSIS GENE WITH LEPIDOPTERAN ACTIVITY 审中-公开
标题翻译：新型苏云金芽孢杆菌基因与鳞翅目动物
公开(公告)号：WO2011084324A2
公开(公告)日：2011-07-14
申请号：PCT/US2010/059467
申请日：2010-12-08
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ABAD, Andre R. , DONG, Hua , KAPKA-KITZMAN, Deirdre M. , LO, Sue B. , SHI, Xiaomei
发明人： ABAD, Andre R. , DONG, Hua , KAPKA-KITZMAN, Deirdre M. , LO, Sue B. , SHI, Xiaomei
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82 , C07K14/325 , C12R1/07
CPC分类号： C07K14/325 , A61K38/00 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera . Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供了从编码具有抗害虫杀虫活性的多肽的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，包括鳞翅目的。本发明的具体实施方案提供了编码杀虫蛋白的分离的核酸，杀虫组合物，DNA构建体和包含实施方案的核酸的转化的微生物和植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

20. 发明申请

WO2011084314A2 NOVEL BACILLUS THURINGIENSIS GENE WITH COLEOPTERAN ACTIVITY 审中-公开
公开(公告)号：WO2011084314A2
公开(公告)日：2011-07-14
申请号：PCT/US2010/059378
申请日：2010-12-08
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ABAD, Andre R. , DONG, Hua , LO, Sue B. , SHI, Xiaomei , WOLFE, Thomas C.
发明人： ABAD, Andre R. , DONG, Hua , LO, Sue B. , SHI, Xiaomei , WOLFE, Thomas C.
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82 , C12R1/07
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

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