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121. 发明申请

WO01083773A1 CLONING AND EXPRESSING AN ACID-RESISTANT EXTRACELLULAR LIPASE OF YARROWIA LIPOLYTICA 审中-公开
标题翻译：克隆和表达YARROWIA LIPOLYTICA的抗酸性细胞外脂质
公开(公告)号：WO01083773A1
公开(公告)日：2001-11-08
申请号：PCT/FR2000/001148
申请日：2000-04-28
IPC分类号： C12N9/20 , C12N15/55 , C12N15/74 , C12N15/79
CPC分类号： C12N9/20
摘要： The invention concerns nucleic acids coding for acid-resistant extracellular lipases, in particular C.ernobii or Y. lipolytica yeasts and the production of said lipases in recombinant form.
摘要翻译：本发明涉及编码耐酸性细胞外脂肪酶，特别是C.ernobii或Y的核酸。解脂耶氏酵母和所述脂肪酶以重组形式的产生。

122. 发明申请

WO0164024B1 MULTIPLE GENE EXPRESSION FOR ENGINEERING NOVEL PATHWAYS AND HYPEREXPRESSION OF FOREIGN PROTEINS IN PLANTS 审中-公开
标题翻译：工程新途径多种基因表达及外源蛋白在植物中的表达
公开(公告)号：WO0164024B1
公开(公告)日：2001-11-01
申请号：PCT/US0106276
申请日：2001-02-28
申请人： UNIV AUBURN , UNIV CENTRAL FLORIDA , DANIELL HENRY , MOAR WILLIAM
发明人： DANIELL HENRY , MOAR WILLIAM
IPC分类号： C07K14/325 , C12N15/79 , C12N15/82 , A01H5/02 , A01H5/10 , A01H13/00 , C12N1/12 , C12N1/20 , C12N5/04 , C12N15/13 , C12N15/17 , C12N15/29 , C12N15/31 , C12N15/32 , C12N15/52 , C12N15/63 , C12N15/74
CPC分类号： C07K14/325 , C12N15/79 , C12N15/8214 , C12N15/8241 , C12N15/8259 , C12N15/8271 , C12N15/8286 , Y02A40/162
摘要： Introducing blocks of foreign genes in a single operon would avoid complications such as position effect and gene silencing inherent in putting one gene at a time into random locations in the nuclear genome. Cloning several genes into a single T-DNA does not avoid the compounded variable expression problem encountered in nuclear transgenic plants. This disclosure shows that a bacterial operon can be expressed in a single integration event as opposed to multiple events requiring several years to accomplish. Expression of multiple genes via a single transformation event opens the possibility of expressing foreign pathways or pharmaceutical proteins involving multiple genes. Expressing the Cry2aA2 operon, including a putative chaperonin to aid in protein folding, in the chloroplast via a single transformation event leads to production of crystalized insecticidal proteins. Expressing the Mer operon via a single transformation event leads to a phytoremediation system.
摘要翻译：在单个操纵子中引入外来基因块可以避免诸如位置效应和将一个基因一次置于核基因组随机位置中固有的基因沉默的并发症。将几个基因克隆成单个T-DNA不能避免在核转基因植物中遇到的复合变量表达问题。本公开表明，细菌操纵子可以在单个整合事件中表达，而不是需要几年才能完成的多个事件。通过单一转化事件表达多个基因，揭示了表达涉及多个基因的外源途径或药物蛋白的可能性。通过单一转化事件表达Cry2aA2操纵子，包括推定的伴侣蛋白以帮助蛋白质折叠，从而产生结晶杀虫蛋白质。通过单一转化事件表达Mer操纵子，导致植物修复系统。

123. 发明申请

WO01077349A1 USE OF 3'-UTR'S FROM CYSTEINE PROTEINASE GENES CPB2 AND CPB2.8 OF LEISHMANIA FOR DIRECTING STAGE-SPECIFIC EXPRESSION 审中-公开
标题翻译：来自CYSTEINE蛋白酶基因的3'-UTR的使用用于指导阶段特异性表达的LEISHMANIA的CPB2和CPB2.8
公开(公告)号：WO01077349A1
公开(公告)日：2001-10-18
申请号：PCT/GB2001/001664
申请日：2001-04-12
IPC分类号： C12N15/67 , C12N15/79 , A61K31/70 , C12N9/50
CPC分类号： C12N15/67 , C12N15/79
摘要： The present invention relates to the identification and use of sequence elements present in the intergenic region of Cysteine Proteinase Genes, cpb , in L. mexicana and the observation that the identified sequences are involved in the control of stage-regulated gene expression. Principal uses include the preparation of vaccines.
摘要翻译：本发明涉及鉴定和使用存在于L型半胱氨酸蛋白酶基因cpb的基因间区域中的序列元件。墨西哥美洲组织和观察到鉴定的序列参与阶段调控基因表达的控制。主要用途包括疫苗的制备。

124. 发明申请

WO01072996A1 A CONSTRUCT CAPABLE OF RELEASE IN CLOSED CIRCULAR FORM FROM A LARGER NUCLEOTIDE SEQUENCE PERMITTING SITE SPECIFIC EXPRESSION AND/OR DEVELOPMENTALLY REGULATED EXPRESSION OF SELECTED GENETIC SEQUENCES 审中-公开
标题翻译：从大型核酸序列允许位点发出封闭式通知的构建能力特定表达和/或开发性调控的选择性遗传序列的表达
公开(公告)号：WO01072996A1
公开(公告)日：2001-10-04
申请号：PCT/AU2001/000349
申请日：2001-03-28
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/79 , C12N15/82
CPC分类号： C12N15/8282 , C12N15/79 , C12N15/8203 , C12N15/8216 , C12N15/8257 , C12N15/8263 , C12N15/8283
摘要： The present invention relates generally to constructs and in particular genetic constructs comprising polynucleotide sequences capable of release in covalently closed, circular form from a larger nucleotide sequence such as, but not limited to, a genome of a eukaryotic cell. Preferably, once released, a polynucleotide sequence is reconstituted in a form which permits expression of the polynucleotide sequence. In one embodiment, the reconstituted polynucleotide sequence comprises a coding sequence with all or part of an extraneous nucleotide such as, but not limited to, intronic sequence or other splice signal inserted therein. Expression and in particular transcription of the coding sequence involves splicing out the extraneous sequence. The release and circularization is generally in response to a stimulus such as a protein-mediated stimulus. More particularly, the protein is a viral or prokaryotic or eukaryotic derived protein or developmentally and/or tissue specific regulated protein. The construct of the present invention is particularly useful in conferring genetic resistance against pathogens or inducing apoptosis or other cell death mechanisms useful, for example, in treating cancer or inducing male or female sterility in plants. The constructs permit, therefore, site specific expression and/or developmentally regulated expression of selected genetic sequences.
摘要翻译：本发明一般涉及构建体，特别是包含能够以较大核苷酸序列例如但不限于真核细胞基因组以共价闭合的环形形式释放的多核苷酸序列的构建体，特别是遗传构建体。优选地，一旦释放，以允许多核苷酸序列表达的形式重构多核苷酸序列。在一个实施方案中，重构的多核苷酸序列包含具有全部或部分外来核苷酸的编码序列，例如但不限于插入其中的内含子序列或其他剪接信号。表达，特别是编码序列的转录涉及剪接外来序列。释放和环化通常响应于诸如蛋白质介导的刺激的刺激。更具体地，蛋白质是病毒或原核或真核衍生的蛋白质或发育和/或组织特异性调节蛋白。本发明的构建体特别可用于赋予病原体的遗传抗性或诱导凋亡或其它细胞死亡机制，例如用于治疗癌症或诱导植物中的雄性或雌性不育。因此，构建体允许选择的遗传序列的位点特异性表达和/或发育调节表达。

125. 发明申请

WO0127287A3 TELOMERASE REVERSE TRANSCRIPTASE (TERT) GENES 审中-公开
标题翻译：端粒酶逆转录酶（TERT）基因
公开(公告)号：WO0127287A3
公开(公告)日：2001-09-07
申请号：PCT/US0027825
申请日：2000-10-10
申请人： RES & DEV I INC , LONG DAVID M , METZ ANNEKE M , LOVE RUSCHELLE
发明人： LONG DAVID M , METZ ANNEKE M , LOVE RUSCHELLE
IPC分类号： C12N9/12 , C12N15/54 , C12N15/79 , C07K16/40 , C12Q1/48 , C12Q1/68 , G01N33/53
CPC分类号： C12N9/1276 , C12N15/79
摘要： The present invention pertains, in general, to the identification, isolation and use of Telomerase Reverse Transcriptase (TERT) genes and the proteins encoded by such genes. In particular, the present invention pertains to the identification, isolation and use of TERT genes and TERT proteins from several genetically diverse and economically important organisms, including two human pathogens and an agronomic crop species.
摘要翻译：本发明一般涉及端粒酶逆转录酶（TERT）基因和由这些基因编码的蛋白质的鉴定，分离和使用。具体而言，本发明涉及鉴定，分离和使用来自几种遗传多样性和经济上重要的生物体（包括两种人类病原体和农学作物物种）的TERT基因和TERT蛋白质。

126. 发明申请

WO01048185A2 TRANSCRIPTION REGULATORY SEQUENCES DERIVED FROM CHLAMYDOMONAS REINHARDTII 审中-公开
标题翻译：从CHLAMYDOMONAS REINHARDTII衍生的转录调节序列
公开(公告)号：WO01048185A2
公开(公告)日：2001-07-05
申请号：PCT/EP2000/013274
申请日：2000-12-27
IPC分类号： C07K14/415 , C12N1/13 , C12N15/79 , C12N15/82 , C12N15/00
CPC分类号： C12N15/8209 , C07K14/415 , C12N15/79 , C12N15/8216 , C12N15/8257
摘要： The invention concerns a transcription regulatory element, characterised in that it comprises: i) the nucleic acid sequence illustrated in Figure 7 (Seq ID n DEG 2), or ii) a fragment thereof, or iii) a sequence having at least 70 % identity with the sequence of Figure 7 (Seq ID n DEG 2).
摘要翻译：本发明涉及转录调节元件，其特征在于其包含：i）图7所示的核酸序列（Seq ID n DEG 2），或ii）其片段，或iii）具有至少70％同一性的序列按照图7的顺序（Seq ID n DEG 2）。

127. 发明申请

WO00062620A3 TRANSFORMED CELLS USEFUL FOR THE CONTROL OF PESTS 审中-公开
标题翻译：用于控制PESTS的变换细胞
公开(公告)号：WO00062620A3
公开(公告)日：2001-07-05
申请号：PCT/US2000/010247
申请日：2000-04-18
IPC分类号： A01N63/04 , A01N65/03 , C07K5/107 , C07K5/113 , C07K7/06 , C07K7/08 , C07K14/435 , A01N63/00 , C07K5/08 , C07K5/10 , C12N15/10 , C12N15/63 , A01K67/027 , C12N15/82 , C12N15/74 , C12N15/79 , A01N37/00
CPC分类号： C07K7/06 , A01N63/00 , A01N63/02 , A01N63/04 , A01N65/00 , C07K5/1016 , C07K5/1021 , C07K7/08 , C07K14/43563 , Y02A50/326 , Y02A50/327
摘要： The subject invention provides materials and methods for the control of pests. Specifically exemplified is the use of recombinant hosts to control mosquito larvae. These hosts, which may be, for example yeast or algae, can be transformed so that they express a pesticidal polypeptide which controls mosquito larvae. These transformed microbes can then be applied to surface waters where mosquito larvae are likely to be found.
摘要翻译：本发明提供了用于控制害虫的材料和方法。具体举例说明的是使用重组宿主来控制蚊子幼虫。可以转化这些宿主，例如酵母或藻类，以便它们表达控制蚊子幼虫的杀虫多肽。然后将这些转化的微生物应用于可能发现蚊子幼虫的地表水中。

128. 发明申请

WO01032896A1 PROTEIN EXPRESSION SYSTEMS FOR NON-PATHOGENIC KINETOPLASTIDAE 审中-公开
标题翻译：非致病性蛋白质表达系统
公开(公告)号：WO01032896A1
公开(公告)日：2001-05-10
申请号：PCT/EP2000/010794
申请日：2000-11-02
IPC分类号： C12N15/09 , C12N1/11 , C12N9/00 , C12N15/79 , C12N15/85
CPC分类号： C12N15/85 , C12N2800/108 , C12N2830/003 , C12N2840/445
摘要： The object of the present invention is a method of recombinant protein production in vitro with cultivated non pathogenic Kinetoplastidae parasites ( Leishmania tarentolae , Crithidia fasciculata , Wallaceina inconstans ) (former Proteomonas inconstans ), ( Leptomonas collos, Leptomonas sp. Cfm, Leptomonas sp. Nfm or Leptomonas seymouri ). This includes description of cultivation conditions for several species that allow large scale growth of these organisms on cheap media. The method of the invention includes strategies and construct description for generation of stably transfected organisms capable of expression of recombinant proteins.
摘要翻译：本发明的目的是在体外用培养的非致病真核型芽胞杆菌寄生虫（即，利什曼原虫，Crithidia）进行重组蛋白质生产的方法 fasciculata ， Wallaceina不饱和）（前蛋白质组蛋白不饱和），（Leptomonas collos，Leptomonas sp。Cfm，Leptomonas sp。Nfm或 Leptomonas seymouri ）。这包括对允许这些生物在廉价媒体上大规模生长的几种物种的栽培条件的描述。本发明的方法包括用于产生能够表达重组蛋白质的稳定转染的生物体的策略和构造描述。

129. 发明申请

WO0061617A3 TRANSIENTLY IMMORTALIZED CELLS FOR USE IN GENE THERAPY 审中-公开
标题翻译：瞬时完全免疫细胞用于基因治疗
公开(公告)号：WO0061617A3
公开(公告)日：2001-04-05
申请号：PCT/US0009775
申请日：2000-04-12
申请人： MODEX THERAPEUTIQUES S A , BAETGE E EDWARD , WONG SHOU , DUPRAZ PHILIPPE , THORENS BERNARD
发明人： BAETGE E EDWARD , WONG SHOU , DUPRAZ PHILIPPE , THORENS BERNARD
IPC分类号： C12N15/09 , A61K35/12 , A61K48/00 , A61P35/00 , C07K14/03 , C07K14/035 , C07K14/155 , C07K14/16 , C07K14/82 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/00 , C12N5/06 , C12N5/10 , C12N9/12 , C12N15/10 , C12N15/62 , C12N15/79
CPC分类号： C07K14/005 , A61K35/12 , A61K48/00 , A61L27/60 , C07K14/82 , C07K2319/10 , C12N9/1276 , C12N2510/04 , C12N2710/16622 , C12N2740/16322
摘要： The invention provides methods and compositions for expanding cells that are not abundant or are difficult to obtain in pure form in culture, are in short supply (e.g., human cells), or have brief lifetimes in culture, using fusion polypeptide. The fusion polypeptide has a first region having the transport function of herpesviral VP22 protein or human immunodeficiency virus (HIV) TAT protein, and a second region with a polypeptide having cell immortalization activity, a polypeptide having telomerase-specific activity, or a polypeptide having telomerase gene activation activity. The resulting cells of the invention are suitable for use in cell therapy.
摘要翻译：本发明提供用于扩增细胞的方法和组合物，所述细胞在培养中不是丰富的或难以获得纯的形式，在短缺（例如人细胞）中，或者使用融合多肽在培养中具有短暂的寿命。融合多肽具有疱疹病毒VP22蛋白或人类免疫缺陷病毒（HIV）TAT蛋白的转运功能的第一区域和具有细胞永生化活性的多肽的第二区域，具有端粒酶活性的多肽或具有端粒酶的多肽基因激活活性。本发明的所得细胞适合用于细胞治疗。

130. 发明申请

WO0011150A8 CELL SURFACE IMMUNOMODULATORS 审中-公开
标题翻译：细胞表面免疫调节剂
公开(公告)号：WO0011150A8
公开(公告)日：2001-02-08
申请号：PCT/US9919386
申请日：1999-08-24
申请人： INCYTE PHARMA INC , LAL PREETI , CORLEY NEIL C , GORGONE GINA A , GUEGLER KARL J , PATTERSON CHANDRA , BAUGHN MARIAH R
发明人： LAL PREETI , CORLEY NEIL C , GORGONE GINA A , GUEGLER KARL J , PATTERSON CHANDRA , BAUGHN MARIAH R
IPC分类号： A61K38/00 , C07K14/705 , C12N15/00 , A61K38/17 , C07H21/00 , C12N15/12 , C12N15/63 , C12N15/74 , C12N15/79
CPC分类号： C07K14/70503 , A61K38/00
摘要： The invention provides human cell surface immunomodulators (CSIMM) and polynucleotides which identify and encode CSIMM. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of CSIMM.
摘要翻译：本发明提供了识别和编码CSIMM的人细胞表面免疫调节剂（CSIMM）和多核苷酸。本发明还提供表达载体，宿主细胞，抗体，激动剂和拮抗剂。本发明还提供了用于诊断，治疗或预防与CSIMM表达相关的疾病的方法。

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