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1. 发明申请

WO2017218875A1 METHOD FOR THE PRODUCTION OF A RHAMNOLIPID 审中-公开
标题翻译：生产RHAMNOLIPID的方法
公开(公告)号：WO2017218875A1
公开(公告)日：2017-12-21
申请号：PCT/US2017/037845
申请日：2017-06-16
申请人： WHITE DOG LABS, INC. , EYAL, Aharon M. , TRACY, Bryan P. , MCWILLIAMS, Christopher Joseph
发明人： EYAL, Aharon M. , TRACY, Bryan P. , MCWILLIAMS, Christopher Joseph
IPC分类号： C12P19/44 , C12R1/385
CPC分类号： C12P19/44 , A61K8/046 , A61K8/602 , A61K9/0014 , A61K9/122 , A61Q19/00 , C07H1/08 , C07H15/04 , C12P7/6409
摘要： A method for the production of a rhamnolipid. The method includes culturing in a fermentation medium an organism capable of producing a rhamnolipid to form a fermentation broth comprising at least one rhamnolipid and having a pH > 5; extracting at least one lipophilic impurity from said fermentation broth; acidulating said fermentation broth to form an aqueous medium of pH
摘要翻译：一种生产鼠李糖脂的方法。该方法包括在发酵培养基中培养能够产生鼠李糖脂的生物以形成包含至少一种鼠李糖脂并且pH> 5; 从所述发酵液中提取至少一种亲脂性杂质; 酸化所述发酵液以形成包含所述至少一种鼠李糖脂的pH <5的水性介质; 从所述酸化的发酵液中提取至少一种鼠李糖脂; 并分离至少一种鼠李糖脂以获得纯化的鼠李糖脂。

2. 发明申请

WO2016160817A1 METHOD OF PRODUCING BIOPRODUCTS 审中-公开
标题翻译：生产生物的方法
公开(公告)号：WO2016160817A1
公开(公告)日：2016-10-06
申请号：PCT/US2016/024730
申请日：2016-03-29
申请人： WHITE DOG LABS, INC.
发明人： TRACY, Bryan P. , MCWILLIAMS, Christopher Joseph , EYAL, Aharon M.
IPC分类号： C07C27/26 , C07C29/86
CPC分类号： C12N1/22 , C07C29/86 , C07C45/80 , C07C51/48 , C07C67/58 , C07D307/50 , C12N1/20 , C12P7/16 , C12P7/24 , C12P7/26 , C12P7/44 , C12P7/52 , C07C31/12 , C07C49/04 , C07C47/02 , C07C55/12 , C07C69/24 , C07C53/122 , C07C55/10
摘要： Methods for production of a bioproduct with a microorganism and selective extraction of bioproducts from a fermentation broth. The methods may include mixing a carbon source, a nitrogen source, and an extractant-depleted raffinate to form a fermentation medium, and fermenting the medium with a microorganism to form a fermentation broth having at least one bioproduct. The bioproduct may be extracted from the fermentation broth with an extractant comprising an oxygenated organic compound and a hydrocarbon to form an extract and a raffinate, and the extract may be further separated from the raffinate. The bioproduct may then be separated from the extract, and the extractant may be separated from the raffinate to regenerate the extract-depleted raffinate.
摘要翻译：用微生物生产生物产物的方法和从发酵液中选择性提取生物产物。所述方法可以包括混合碳源，氮源和提取物贫化的提余液以形成发酵培养基，并用微生物发酵培养基以形成具有至少一种生物产物的发酵液。生物产物可以用包含含氧有机化合物和烃的提取剂从发酵液中提取以形成提取物和萃余液，并且提取物可以进一步与萃余液分离。然后可以将生物产物与提取物分离，并且萃取剂可以与萃余液分离，以再生提取物贫化的残液。

3. 发明申请

WO2016205204A1 METHOD FOR PRODUCING ONE OR MORE OIL-SOLUBLE BIOPRODUCTS 审中-公开
标题翻译：生产一种或多种油溶性生物的方法
公开(公告)号：WO2016205204A1
公开(公告)日：2016-12-22
申请号：PCT/US2016/037385
申请日：2016-06-14
申请人： WHITE DOG LABS, INC.
发明人： EYAL, Aharon M. , TRACY, Bryan P. , MCWILLIAMS, Christopher Joseph
IPC分类号： C07C29/86 , C10G3/00 , C10L1/02 , C11B1/10
CPC分类号： C11B1/104 , C07C7/10 , C07C11/18 , C11B1/10 , C12P5/026 , C12P7/6409
摘要： Disclosed are methods for making one or more bioproducts such as isoprene derivatives, fatty acids, fatty acid derivatives, fatty alcohols, fatty alcohol derivatives, mixtures thereof, and compositions having one or more such bioproducts with microorganisms and for separating the bioproduct(s) or bioproduct composition(s). The methods include providing an extractant having a solvent with a boiling point at atmospheric pressure of less than 20°C; feeding an aqueous suspension of cells that have produced the bioproduct(s) into a pressurized column; feeding the extractant into the pressurized column; removing an extract having the extractant and the bioproduct(s) from said pressurized column; and separating the extractant from the removed extract to obtain a separated oil-soluble composition having the bioproduct.
摘要翻译：公开了制备一种或多种生物产物的方法，例如异戊二烯衍生物，脂肪酸，脂肪酸衍生物，脂肪醇，脂肪醇衍生物，其混合物，以及具有一种或多种这样的生物产物与微生物并用于分离生物产物或生物制品组合物。所述方法包括提供具有沸点在大气压低于20℃的溶剂的萃取剂; 将已经生产生物产物的细胞的水性悬浮液加入加压柱中; 将萃取剂进料到加压柱中; 从所述加压塔中除去具有萃取剂和生物产物的提取物; 并将萃取剂与去除的提取物分离，得到具有生物产物的分离的油溶性组合物。

4. 发明申请

WO2016160812A1 METHOD OF PRODUCING BIOPRODUCTS 审中-公开
标题翻译：生产生物的方法
公开(公告)号：WO2016160812A1
公开(公告)日：2016-10-06
申请号：PCT/US2016/024724
申请日：2016-03-29
申请人： WHITE DOG LABS, INC.
发明人： TRACY, Bryan P. , MCWILLIAMS, Christopher Joseph , EYAL, Aharon M.
IPC分类号： C07C27/26 , C07C29/86
CPC分类号： C12P7/52 , B01D11/00 , B01D11/02 , C07C29/86 , C07C45/80 , C07C51/48 , C07C67/58 , C07D307/50 , C12P7/04 , C12P7/16 , C12P7/26 , C12P7/44 , C07C31/12 , C07C31/125 , C07C49/04 , C07C47/02 , C07C55/12 , C07C69/24 , C07C53/122 , C07C55/10
摘要： Methods for production of a bioproduct with a microorganism and selective extraction of bioproducts from a fermentation broth. The methods may include mixing a carbon source, a nitrogen source, and an extractant-depleted raffinate to form a fermentation medium, and fermenting the medium with a microorganism to form a fermentation broth having at least one bioproduct. The bioproduct may be extracted from the fermentation broth with an extractant comprising an olefin to form an extract and a raffinate, and the extract may be further separated from the raffinate. The bioproduct may then be separated from the extract, and the extractant may be separated from the raffinate to regenerate the extract-depleted raffinate.
摘要翻译：用微生物生产生物产物的方法和从发酵液中选择性提取生物产物。所述方法可以包括混合碳源，氮源和提取物贫化的提余液以形成发酵培养基，并用微生物发酵培养基以形成具有至少一种生物产物的发酵液。可以使用包含烯烃的提取剂从发酵液中提取生物产物以形成提取物和萃余液，并且提取物可进一步与萃余液分离。然后可以将生物产物与提取物分离，并且萃取剂可以与萃余液分离，以再生提取物贫化的残液。

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