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    • 2. 发明申请
    • DETECTION METHOD BY PCR
    • PCR检测方法
    • WO0131056A3
    • 2002-06-27
    • PCT/BE0000131
    • 2000-10-27
    • UNIV LIEGEZORZI WILLYMELEN LAURENCEZORZI DANIELEEL MOUALIJ BENAISSAHEINEN ERNST
    • ZORZI WILLYMELEN LAURENCEZORZI DANIELEEL MOUALIJ BENAISSAHEINEN ERNST
    • C12Q1/68G01N33/50G01N33/543
    • C12Q1/686C12Q1/6804
    • The invention concerns a method using PCR, in particular immuno-PCR, for detecting microbiological entities and in particular elements or target molecules. The detection method comprises a step which consists in fixing an entity A, more particularly an antigen, directly or via a capture agent, for example an antibody, to one or several surface(s) inside the receptacle(s) of a real time PCR measuring apparatus and a step which consists in associating with said element A a coupling system with a nucleic acid, for example a DNA marker, a step which consists in amplifying the marker nucleic acid. The successive measurements are carried out during the phase amplifying the marker nucleic acid, in the presence of a capture agent and other optional intermediate fixing agents. The detection method is quantitative and can be used in particular for prion polypeptide assay.
    • 本发明涉及使用PCR,特别是免疫PCR的方法来检测微生物实体,特别是元件或靶分子。 检测方法包括将实体A,特别是抗原直接或经由捕获剂(例如抗体)固定在实时PCR中的一个或多个容器内的一个或多个表面的步骤 测量装置和步骤,其包括与所述元件A结合与核酸(例如DNA标记)的偶联系统,其包含扩增标记核酸的步骤。 在捕获剂和其它任选的中间固定剂的存在下,在标记核酸的相扩增期间进行连续的测量。 检测方法是定量的,可以特别用于朊病毒多肽测定。