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1. 发明申请

WO2011105841A3 RECOMBINANT EXPRESSION VECTOR FOR EXPRESSING BRAZZEIN, AND NOVEL MULTIPLE MUTATION BRAZZEIN VARIANT HAVING GREATER SWEETNESS 审中-公开
标题翻译：用于表达布拉泽星的重组表达载体，以及具有较大尺寸的新型多重突变布拉泽宁变化
公开(公告)号：WO2011105841A3
公开(公告)日：2012-04-05
申请号：PCT/KR2011001309
申请日：2011-02-24
申请人： UNIV CHUNG ANG IND , KONG KWANG HOON
发明人： KONG KWANG HOON
IPC分类号： C12N15/29 , C07K14/415 , C12N15/70 , C12N15/81 , C12P21/02
CPC分类号： C07K14/43 , C12N15/815 , C12P21/02
摘要： The present invention relates to a recombinant expression vector for expressing brazzein and to a method for the mass production of brazzein using the vector, in which a brazzein expression system is constructed in Kluyveromyces lactis, which is a lactose-fermenting yeast, and particularly, in which an optimum condition is established to achieve significantly superior expression than conventional E. coli or yeast expression systems, thus enabling the mass production of brazzein. In addition, the present invention relates to a novel biquadratic brazzein variant having greater sweetness, which has the same thermal stability as that of conventional brazzein, and a sweetness that is at least 15 to 50 times higher than that of conventional brazzein. Therefore, the brazzein variant of the present invention can be used together with other sweeteners such as sugar (sucrose), and further less brazzein variant may be used as a substitute for the same amount of sugar, and can be variously used as additives in food production.
摘要翻译：本发明涉及用于表达brazzein的重组表达载体和使用该载体大规模生产brazzein的方法，其中在乳糖发酵酵母中的克鲁维酵母克鲁维酵母构建了brazzein表达系统，特别是在其建立了最佳条件以实现比常规大肠杆菌或酵母表达系统显着优异的表达，从而使得大量生产brazzein。此外，本发明涉及一种具有较高甜度的新颖的双二价乳酪蛋白变体，其具有与常规乳糖凝胶相同的热稳定性，并且甜度比常规乳糖凝胶的甜度高至少15至50倍。因此，本发明的纤维素变体可以与其他甜味剂如糖（蔗糖）一起使用，而更少的乳糖变体可用作相同量的糖的替代物，并且可以各种用作食物中的添加剂生产。

2. 发明申请

WO2011105841A9 RECOMBINANT EXPRESSION VECTOR FOR EXPRESSING BRAZZEIN, AND NOVEL MULTIPLE MUTATION BRAZZEIN VARIANT HAVING GREATER SWEETNESS 审中-公开
标题翻译：用于表达BRAZZEIN的重组表达载体，以及具有更大的甜度的新的多突变BRAZZEIN变体
公开(公告)号：WO2011105841A9
公开(公告)日：2012-01-26
申请号：PCT/KR2011001309
申请日：2011-02-24
申请人： UNIV CHUNG ANG IND , KONG KWANG HOON
发明人： KONG KWANG HOON
IPC分类号： C12N15/29 , C07K14/415 , C12N15/70 , C12N15/81 , C12P21/02
CPC分类号： C07K14/43 , C12N15/815 , C12P21/02
摘要： The present invention relates to a recombinant expression vector for expressing brazzein and to a method for the mass production of brazzein using the vector, in which a brazzein expression system is constructed in Kluyveromyces lactis, which is a lactose-fermenting yeast, and particularly, in which an optimum condition is established to achieve significantly superior expression than conventional E. coli or yeast expression systems, thus enabling the mass production of brazzein. In addition, the present invention relates to a novel biquadratic brazzein variant having greater sweetness, which has the same thermal stability as that of conventional brazzein, and a sweetness that is at least 15 to 50 times higher than that of conventional brazzein. Therefore, the brazzein variant of the present invention can be used together with other sweeteners such as sugar (sucrose), and further less brazzein variant may be used as a substitute for the same amount of sugar, and can be variously used as additives in food production.
摘要翻译：本发明涉及用于表达布拉霉素的重组表达载体和使用该载体大量生产布拉霉素的方法，其中在作为乳糖发酵酵母的乳酸克鲁维酵母中构建了布丁蛋白表达系统，并且特别地，其中建立了最佳条件以实现比常规大肠杆菌或酵母表达系统显着优越的表达，从而能够大规模生产brazzein。此外，本发明涉及一种具有更高甜度的新颖的双四方形Brazzein变体，其具有与常规Brazzein相同的热稳定性，并且甜度比常规Brazzein高至少15至50倍。因此，本发明的布氏蛋白变体可以与其他甜味剂如糖（蔗糖）一起使用，并且可以使用更少的布氏蛋白变体作为相同量的糖的替代物，并且可以不同地用作食物中的添加剂生产。

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