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1. 发明申请

WO2015126899A2 PROCESSES AND APPARATUS FOR REMOVAL OF FERMENTATION INHIBITORS FROM BIOMASS HYDROLYSATES 审中-公开
标题翻译：用于从生物质水解产物中去除发酵抑制剂的方法和设备
公开(公告)号：WO2015126899A2
公开(公告)日：2015-08-27
申请号：PCT/US2015/016308
申请日：2015-02-18
申请人： API INTELLECTUAL PROPERTY HOLDINGS, LLC
发明人： RETSINA, Theodora , MONCLIN, Jean-Pierre , ZEBROSKI, Ryan , TRYPAKIS, Anastasios , PYLKKANEN, Vesa
IPC分类号： C08B15/00
CPC分类号： C08B15/00 , C08H8/00
摘要： The disclosure provides a process for separating fermentation inhibitors from a biomass-derived hydrolysate, comprising: introducing a biomass-derived liquid hydrolysate stream to a stripping column; introducing a steam-rich vapor stream to the stripping column to strip fermentation inhibitors (such as acetic acid) from the liquid hydrolysate stream; recovering a stripped liquid stream and a stripper vapor output stream; compressing the stripper vapor output stream; introducing the compressed vapor stream, and a water-rich liquid stream, to an evaporator; recovering, from the evaporator, an evaporated liquid stream and an evaporator output vapor stream; and recycling the evaporator output vapor stream to the stripping column as the steam-rich vapor stream. Other variations utilize a rectification column to recover a rectified liquid stream and a rectification column vapor stream, and recycle the rectification column vapor stream to the stripping column as the steam-rich vapor stream.
摘要翻译：本公开提供了一种从生物质衍生的水解产物中分离发酵抑制剂的方法，包括：将生物质衍生的液体水解产物流引入汽提塔; 将富含蒸汽的蒸气流引入汽提塔以从液体水解产物流中汽提发酵抑制剂（如乙酸）; 回收经汽提的液体流和汽提器蒸汽输出流; 压缩汽提器蒸汽输出流; 将压缩蒸气流和富水液流引入蒸发器; 从蒸发器回收蒸发的液体流和蒸发器输出蒸汽流; 并将蒸发器输出蒸汽流作为富含蒸汽的蒸汽流再循环至汽提塔。其他变型使用精馏塔来回收经精馏的液体物流和精馏塔蒸气物流，并且将精馏塔蒸气物流作为富含蒸汽的蒸汽物流循环至汽提塔。

2. 发明申请

WO2017205705A1 SYSTEMS AND METHODS FOR CONTINUOUSLY FERMENTING C5 AND C6 SACCHARIDES 审中-公开
标题翻译：用于连续引发C5和C6糖链的系统和方法
公开(公告)号：WO2017205705A1
公开(公告)日：2017-11-30
申请号：PCT/US2017/034596
申请日：2017-05-26
申请人： API INTELLECTUAL PROPERTY HOLDINGS, LLC
发明人： SURVASE, Shrinkant , ZEBROSKI, Ryan , ADAMOS, Georgios , PYLKKANEN, Vesa
IPC分类号： C12P7/04 , C12P7/06 , C12P7/14 , C12P7/16 , C12P7/28 , C12Q3/00
CPC分类号： C12P7/10 , C12P7/04 , C12P7/16 , C12P7/28 , C12P7/52 , C12P7/54 , C12P19/02 , C12P19/14 , C12P2201/00 , C12P2203/00 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield. Some variations provide a method of fermenting C 5 and C 6 sugars to fermentation products, the method comprising: fermenting a C 6 -rich sugar feed to a first fermentation product; fermenting a C 5 -rich sugar feed to a second fermentation product; removing microorganism cells from the first fermentor, to maintain a cell concentration within a selected range; conveying microorganism cells to a second fermentor; and removing microorganism cells from the second fermentor, to maintain a microorganism cell concentration that is greater than that in the C 6 -rich fermentor.
摘要翻译：本发明提供了纤维素和半纤维素糖的优化发酵。利用微生物，代谢中间体和营养物质的再利用和再循环，将生物质衍生的半纤维素和纤维素糖独立调节并分别发酵。条件糖可以在单独的容器中发酵，其中来自葡萄糖发酵的过量细胞与来自溶剂回收的提余液一起被输送到半纤维素糖发酵，以提高生产率和产品产量。一些变化提供了将C 5和C 6糖发酵成发酵产物的方法，所述方法包括：发酵富含C 6的糖进料到第一发酵产物; 将第C 5高丰度糖原料发酵成第二发酵产物; 从第一发酵罐中除去微生物细胞，以将细胞浓度保持在选定范围内; 将微生物细胞输送至第二发酵罐; 并从第二发酵罐中除去微生物细胞，以保持比富含Cys6的发酵罐更高的微生物细胞浓度。

3. 发明申请

WO2015100445A1 PRODUCTION OF FERMENTABLE C5 AND C6 SUGARS FROM LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：从Lignocelluliosic生物量生产可食用的C5和C6糖
公开(公告)号：WO2015100445A1
公开(公告)日：2015-07-02
申请号：PCT/US2014/072453
申请日：2014-12-27
申请人： API INTELLECTUAL PROPERTY HOLDINGS, LLC
发明人： RETSINA, Theodora , PYLKKANEN, Vesa , ZEBROSKI, Ryan
IPC分类号： C12P19/02 , C08B1/00
CPC分类号： C13K1/02 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C12P2203/00 , C13K13/002 , Y02E50/16
摘要： In some variations, this invention provides a process for producing fermentable sugars from cellulosic biomass, comprising: extracting biomass with steam or hot water to produce an extract liquor containing hemicellulose oligomers, dissolved lignin, and cellulose-rich solids; separating and washing the cellulose-rich solids; removing a portion of glucan contained in the washed cellulose-rich solids as glucose oligomers using a liquefaction-focused blend of enzymes; co-hydrolyzing glucose oligomers and hemicellulose oligomers, with enzymes or chemical catalyst, to produce glucose and hemicellulose monomers; and recovering the glucose and hemicellulose monomers as fermentable sugars. The liquefaction-focused blend of enzymes contains endoglucanases and exoglucanases. Optionally, the glucose and the hemicellulose monomers may be recovered as separate streams. The residual cellulose (not hydrolyzed) as well as the lignin may be recovered and combusted, or utilized for other purposes, such as for energy-dense pellets.
摘要翻译：在一些变型中，本发明提供了一种从纤维素生物质生产可发酵糖的方法，其包括：用蒸汽或热水提取生物质以产生含有半纤维素低聚物，溶解的木质素和富含纤维素的固体的提取液; 分离和洗涤富含纤维素的固体; 使用液化聚焦的酶将所洗涤的富含纤维素的固体中含有的一部分葡聚糖作为葡萄糖寡聚物除去; 用酶或化学催化剂共同水解葡萄糖寡聚体和半纤维素寡聚物以产生葡萄糖和半纤维素单体; 并将葡萄糖和半纤维素单体作为可发酵糖回收。液化聚焦的混合物含有内切葡聚糖酶和外切葡聚糖酶。任选地，葡萄糖和半纤维素单体可以作为单独的流回收。残留的纤维素（未水解）以及木质素可以回收和燃烧，或用于其它目的，例如用于能量密度的颗粒。

4. 发明申请

WO2015100444A1 PROCESSES AND APPARATUS FOR PRODUCING FERMENTABLE SUGARS FROM BIOMASS BY HOT-WATER EXTRACTION AND ENZYMATIC HYDROLYSIS 审中-公开
标题翻译：通过热水提取和酶水解从生物量生产可食用糖的工艺和设备
公开(公告)号：WO2015100444A1
公开(公告)日：2015-07-02
申请号：PCT/US2014/072452
申请日：2014-12-27
申请人： API INTELLECTUAL PROPERTY HOLDINGS, LLC
发明人： ZEBROSKI, Ryan , SCHULTZE, James
IPC分类号： C12P19/02 , C08B1/00
CPC分类号： C13K1/02 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C13K1/04 , Y02E50/16
摘要： The present invention is capable of producing glucose and hemicellulose sugars from lignocellulosic biomass. In some variations, a process includes optionally pre-steaming a biomass feedstock; extracting the feedstock with liquid hot water to produce glucan-rich solids and an extract liquor containing dissolved solids, which include hemicellulosic oligomers and lignin; washing the glucan-rich solids; hydrolyzing the hemicellulosic oligomers by contacting the extract liquor with an acid catalyst or enzymes possessing hemicellulase activity; separately hydrolyzing the glucan by contacting the glucan-rich solids stream with an acid catalyst or enzymes possessing glucanase activity, optionally with removing the glucose in situ by microfiltration and/or ultrafiltration; and recovering or fermenting each of the hemicellulosic monomers and the glucose. Preferred configurations and conditions are disclosed.
摘要翻译：本发明能够从木质纤维素生物质生产葡萄糖和半纤维素糖。在一些变型中，方法包括任选地预蒸汽生物质原料; 用液体热水提取原料以产生富含葡聚糖的固体，以及含有溶解的固体的提取液，包括半纤维素低聚物和木质素; 洗涤富含葡聚糖的固体; 通过使萃取液与酸催化剂或具有半纤维素酶活性的酶接触来水解半纤维素低聚物; 通过使富含葡聚糖的固体物流与酸催化剂或具有葡聚糖酶活性的酶接触来分别水解葡聚糖，任选地通过微量过滤和/或超滤原位除去葡萄糖; 并回收或发酵每种半纤维素单体和葡萄糖。公开了优选的配置和条件。

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