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1. 发明申请

WO2010099264A2 CELL CULTURE SYSTEM WITH MANIFOLD 审中-公开
标题翻译：细胞培养系统
公开(公告)号：WO2010099264A2
公开(公告)日：2010-09-02
申请号：PCT/US2010/025321
申请日：2010-02-25
申请人： CORNING INCORPORATED , KENNEY, David, A. , MARTIN, Gregory, R. , WALL, Joseph, C.
发明人： KENNEY, David, A. , MARTIN, Gregory, R. , WALL, Joseph, C.
IPC分类号： C12M1/24
CPC分类号： C12M23/08 , C12M23/34 , C12M23/40 , C12M23/44 , Y10T29/49826
摘要： A cell culture apparatus includes cell culture units having cell culture chambers, each with at least one manifold. The manifold connects the cell culture unit to a fluid flow channel. The manifold has a dam which allows liquid to pool in the manifold and allows for the creation of an airspace in the manifold, which reduces hydrostatic pressure inside the apparatus and enables the stacking of multiple cell culture units. Embodiments include cascading manifolds, manifolds having reservoirs, and manifolds having valves.
摘要翻译：细胞培养装置包括具有细胞培养室的细胞培养单元，每个细胞培养室具有至少一个歧管。歧管将细胞培养单元连接到流体流动通道。歧管具有允许液体在歧管中汇集并且允许在歧管中创建空气空间的坝，这降低了设备内部的静水压力，并且能够堆叠多个细胞培养单元。实施例包括级联歧管，具有储存器的歧管和具有阀的歧管。

2. 发明申请

WO2009148512A3 ASSEMBLY OF CELL CULTURE VESSELS 审中-公开
公开(公告)号：WO2009148512A3
公开(公告)日：2009-12-10
申请号：PCT/US2009/003127
申请日：2009-05-20
申请人： CORNING INCORPORATED , CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
发明人： CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
IPC分类号： B29C65/14 , C12M1/14
摘要： A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.

3. 发明申请

WO2009145875A1 METHOD FOR ULTRASONIC CELL REMOVAL 审中-公开
标题翻译：超声波清除方法
公开(公告)号：WO2009145875A1
公开(公告)日：2009-12-03
申请号：PCT/US2009/003092
申请日：2009-05-19
申请人： CORNING INCORPORATED , MARTIN, Gregory, R. , TANNER, Allison, J.
发明人： MARTIN, Gregory, R. , TANNER, Allison, J.
IPC分类号： C12M1/42 , C12M3/00
CPC分类号： C12M33/12 , C12M23/04 , C12M23/08 , C12M23/24 , C12M23/34 , C12M23/44
摘要： The present invention provides a method for treating cell culture vessels including multi-layer cell culture vessels with ultrasonic energy to dissociate cells growing on surfaces of cell culture vessels.
摘要翻译：本发明提供一种处理细胞培养容器的方法，其包括具有超声能量的多层细胞培养容器，以解离细胞培养容器表面生长的细胞。

4. 发明申请

WO2008005244A3 HIGH DENSITY PERMEABLE SUPPORTS FOR HIGH THROUGHPUT SCREENING 审中-公开
标题翻译：高密度渗透性支持用于高通量筛选
公开(公告)号：WO2008005244A3
公开(公告)日：2008-04-03
申请号：PCT/US2007014852
申请日：2007-06-27
申请人： CORNING INC , MARTIN GREGORY R , TANNER ALLISON J
发明人： MARTIN GREGORY R , TANNER ALLISON J
IPC分类号： B01L3/00 , C12M3/06
CPC分类号： C12M25/04 , B01L3/50255 , B01L3/50857 , B01L2300/0681 , B01L2300/0829 , C12M23/24 , C12M23/38
摘要： A class of designs is provided for a permeable support where the design principle includes a permeable support layer (250), such as a membrane, bonded midway between two arrays of wells (110, 130) or multiwell plates, attached at their bottom portions, forming a double-sided multiwell plate. Thus, opposite facing wells on either side of the permeable support layer are accessible by inversion of the double-sided multiwell plate. Well fluid is held in place in the novel multiwell plate by capillary forces in the case of aligned upper and lower well arrays or by surface tension on patterned well regions on a permeable membrane layer. No additional components are necessary to form compartments for fluid retention. These new plate designs allow the surface area of the permeable support layer to be maximized, eliminate potential wicking and. cross contamination issues that may arise from multiple component sidewalls, and take advantage of the small well diameters to retain fluid by utilizing the effects of surface tension or capillary forces.
摘要翻译：提供了一类用于可渗透支撑件的设计，其中设计原理包括在其底部附接的两个井组（110,130）或多孔板之间的中间的粘合剂（例如膜）的可渗透支撑层（250）形成双面多孔板。因此，可渗透支撑层的任一侧上的相对面对的孔可通过双面多孔板的倒置进入。在新颖的多孔板中，在对准的上下阵列的情况下通过毛细作用力将液体保持在适当的位置，或者通过可渗透膜层上的图案化阱区域上的表面张力来保持良好的流体。不需要额外的部件来形成用于流体保持的隔室。这些新的板设计允许可渗透支撑层的表面积最大化，消除潜在的芯吸。可能由多个部件侧壁产生的交叉污染问题，并且通过利用表面张力或毛细管力的作用，利用小孔直径来保留流体。

5. 发明申请

WO2010099264A3 CELL CULTURE SYSTEM WITH MANIFOLD 审中-公开
标题翻译：具有歧管的细胞培养系统
公开(公告)号：WO2010099264A3
公开(公告)日：2010-12-02
申请号：PCT/US2010025321
申请日：2010-02-25
申请人： CORNING INC , KENNEY DAVID A , MARTIN GREGORY R , WALL JOSEPH C
发明人： KENNEY DAVID A , MARTIN GREGORY R , WALL JOSEPH C
IPC分类号： C12M1/24 , B01L3/00
CPC分类号： C12M23/08 , C12M23/34 , C12M23/40 , C12M23/44 , Y10T29/49826
摘要： A cell culture apparatus includes cell culture units having cell culture chambers, each with at least one manifold. The manifold connects the cell culture unit to a fluid flow channel. The manifold has a dam which allows liquid to pool in the manifold and allows for the creation of an airspace in the manifold, which reduces hydrostatic pressure inside the apparatus and enables the stacking of multiple cell culture units. Embodiments include cascading manifolds, manifolds having reservoirs, and manifolds having valves.
摘要翻译：细胞培养装置包括具有细胞培养室的细胞培养装置，每个细胞培养装置具有至少一个歧管。歧管将细胞培养单元连接到流体流动通道。该歧管具有允许液体汇集在歧管中并且允许在歧管中产生空气空间的坝，其减小了设备内部的静水压力并且能够堆叠多个细胞培养单元。实施例包括级联歧管，具有储存器的歧管和具有阀的歧管。

6. 发明申请

WO2009148512A2 ASSEMBLY OF CELL CULTURE VESSELS 审中-公开
标题翻译：细胞培养血管的组装
公开(公告)号：WO2009148512A2
公开(公告)日：2009-12-10
申请号：PCT/US2009003127
申请日：2009-05-20
申请人： CORNING INC , CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
发明人： CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
IPC分类号： C12M1/00 , C12M1/14
CPC分类号： B29C65/1629 , B29C45/0053 , B29C45/16 , B29C65/08 , B29C65/14 , B29C65/1412 , B29C65/1454 , B29C65/1477 , B29C65/1616 , B29C65/1654 , B29C65/1664 , B29C65/1674 , B29C65/4815 , B29C65/4855 , B29C66/112 , B29C66/1122 , B29C66/114 , B29C66/5346 , B29C66/542 , B29C66/71 , B29C66/712 , B29C66/7352 , B29C2035/0822 , B29C2045/0079 , B29K2009/06 , B29K2023/00 , B29K2023/06 , B29K2023/083 , B29K2023/12 , B29K2025/00 , B29K2027/06 , B29K2027/12 , B29K2027/18 , B29K2033/12 , B29K2067/00 , B29K2069/00 , B29K2077/00 , B29K2081/06 , B29K2995/0065 , B29K2995/0069 , C12M23/08 , C12M23/20 , C12M23/24 , C12M23/44 , B29K2083/005 , B29K2025/08 , B29K2025/06 , B29K2023/38 , B29K2023/18 , B29K2023/14 , B29K2023/0616
摘要： A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.
摘要翻译：细胞培养装置包括由第一主表面，与第一主表面间隔开的相对的第二主表面形成的细胞培养室，以及围绕第一腔室并在第一和第二主表面之间延伸的第一侧壁。靠近第一主表面的第一侧壁的一部分包括红外吸收材料，并且靠近第二主表面的第一侧壁的部分由基本上非红外吸收材料形成。第一主表面是由细胞培养液不渗透的透气性聚合物膜形成的。

7. 发明申请

WO2009128894A3 FLOAT VALVE FOR CELL CULTURE VESSEL 审中-公开
公开(公告)号：WO2009128894A3
公开(公告)日：2009-10-22
申请号：PCT/US2009/002304
申请日：2009-04-14
申请人： CORNING INCORPORATED , CATTADORIS, Henry, J. , MARTIN, Gregory, R , TANNER, Allison, J
发明人： CATTADORIS, Henry, J. , MARTIN, Gregory, R , TANNER, Allison, J
IPC分类号： C12M1/00
摘要： A cell culture vessel includes a housing defining a reservoir, an inlet and an outlet in fluid communication with the reservoir, a valve assembly, and an antimicrobial filter. The valve assembly includes (i) a side wall defining a passageway for fluid flow between the reservoir and the outlet, (ii) a floating element disposed in the passageway and configured to float on the culture medium, (iii) a stop feature configured to sealingly engage the floating element to prevent the culture medium from flowing from the reservoir through the outlet, and (iv) a capture feature configured to retain the floating element within the passageway and to allow fluid to flow from the reservoir through the passageway. The microbial filter is positioned such that air flowing in the outlet to the passageway passes through the filter.

8. 发明申请

WO2009094125A3 LIMITED ACCESS MULTI-LAYER CELL CULTURE SYSTEM 审中-公开
标题翻译：有限访问多层细胞培养系统
公开(公告)号：WO2009094125A3
公开(公告)日：2010-08-26
申请号：PCT/US2009000303
申请日：2009-01-16
申请人： CORNING INC , MARTIN GREGORY R , TANNER ALLISON J
发明人： MARTIN GREGORY R , TANNER ALLISON J
IPC分类号： C12M1/14
CPC分类号： C12M23/34 , C12M23/04 , C12M23/08 , C12M23/38 , C12M23/40
摘要： The present invention provides a multi-layer cell culture device having a rectangular footprint and having multiple cell culture chambers separated by tracheal air spaces, each cell culture chamber having a port and a port cover or an external manifold structured and arranged to allow for transfer of fluid into and out of the cell culture device with reduced risk of contamination, and methods of using the device.
摘要翻译：本发明提供了一种具有矩形足迹并具有由气管空气间隔开的多个细胞培养室的多层细胞培养装置，每个细胞培养室具有端口和端口盖或外部歧管，其构造和布置成允许流入和流出细胞培养装置的污染风险降低，以及使用该装置的方法。

9. 发明申请

WO2010024887A1 CELL CULTURE APPARATUS AND METHOD 审中-公开
标题翻译：细胞培养装置及方法
公开(公告)号：WO2010024887A1
公开(公告)日：2010-03-04
申请号：PCT/US2009/004843
申请日：2009-08-26
申请人： CORNING INCORPORATED , MARTIN, Gregory, R , TANNER, Allison J
发明人： MARTIN, Gregory, R , TANNER, Allison J
IPC分类号： C12M1/00
CPC分类号： C12M23/24 , C12M23/14 , C12M23/34
摘要： Cell culture apparatuses and methods for culturing cells include bags for culturing cells. The bags are folded and unfolded to control cell culture conditions. As the bags are folded, segmented sealed chambers for culturing cells are formed. As the bags are unfolded, the segments are unsealed allowing for exchange of fluid between the regions of the subchambers.
摘要翻译：培养细胞的细胞培养装置和方法包括培养细胞的袋。将袋折叠并展开以控制细胞培养条件。当袋子被折叠时，形成用于培养细胞的分段密封室。当袋子展开时，这些片段是开封的，允许在子房间的区域之间交换流体。

10. 发明申请

WO2009128892A3 FLEXIBLE MEMBRANE VALVE FOR CELL CULTURE VESSEL 审中-公开
公开(公告)号：WO2009128892A3
公开(公告)日：2009-10-22
申请号：PCT/US2009/002302
申请日：2009-04-14
申请人： CORNING INCORPORATED , CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
发明人： CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
IPC分类号： C12M1/12 , C12M1/22 , C12M1/24
摘要： An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir. The slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold. The filter is disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter.

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