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1. 发明申请

WO2015063489A1 APPARATUS AND METHOD FOR FILTRATION OF A SUSPENSION 审中-公开
标题翻译：用于过滤悬浮液的装置和方法
公开(公告)号：WO2015063489A1
公开(公告)日：2015-05-07
申请号：PCT/GB2014/053227
申请日：2014-10-30
申请人： EXMOOR PHARMA CONCEPTS LTD , LONZA BIOLOGICS PLC , UCL BUSINESS PLC , AMERCARE LTD
发明人： VINCENT, John , TOSTOES, Rui , VERAITCH, Farlan , DODGSON, John
IPC分类号： B01D63/08 , B01L3/00 , C12M1/00
CPC分类号： C12N5/0688 , A61K35/42 , B01D63/087 , B01D2313/10 , B01D2313/12 , B01D2315/14 , B01D2315/16 , C12M47/02 , G01N2001/4088
摘要： The present invention provides devices suitable for filtration including diafiltration of a suspension containing a species such as particles, cells or macromolecules, apparatus comprising such devices and methods of using the devices and apparatus. The devices of the invention comprise a vessel having a filter that divides the vessel into a first chamber and a second chamber provided with various outlet ports. Gas is used to finally concetrate the suspension (118) in the filtration chamber by displacing the liquid up to 95% through the filter (14).
摘要翻译：本发明提供了适用于过滤的装置，包括含有诸如颗粒，细胞或大分子之类的悬浮液的渗滤，包括这种装置的装置以及使用该装置和装置的方法。本发明的装置包括具有将容器分成第一室的过滤器的容器和具有各种出口的第二室。通过使液体通过过滤器（14）将液体置换成95％，气体最终使过滤室中的悬浮液（118）混合。

2. 发明申请

WO2010089151A8 BIOREACTOR FOR THE CULTIVATION OF MAMMALIAN CELLS 审中-公开
标题翻译：用于培育哺乳动物细胞的生物反应器
公开(公告)号：WO2010089151A8
公开(公告)日：2010-11-18
申请号：PCT/EP2010000783
申请日：2010-02-09
申请人： LONZA BIOLOGICS PLC , KHAN MOSHAN
发明人： KHAN MOSHAN
IPC分类号： C12M1/02 , C12M1/06 , C12M1/107 , C12M1/113
CPC分类号： C12M23/58 , C12M21/08 , C12M27/02 , C12M27/08 , C12M27/20 , C12M29/06 , C12N5/00 , C12N2527/00
摘要： The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
摘要翻译：本发明涉及具有至少两个叶轮的大型生物反应器，大规模生物反应器系统和使用这些生物反应器大规模培养和繁殖哺乳动物细胞的方法。

3. 发明申请

WO2010089151A1 BIOREACTOR FOR THE CULTIVATION OF MAMMALIAN CELLS 审中-公开
标题翻译：用于培养马马龙细胞的生物反应器
公开(公告)号：WO2010089151A1
公开(公告)日：2010-08-12
申请号：PCT/EP2010/000783
申请日：2010-02-09
申请人： LONZA BIOLOGICS PLC. , KHAN, Moshan
发明人： KHAN, Moshan
IPC分类号： C12M1/02 , C12M1/06 , C12M1/107 , C12M1/113
CPC分类号： C12M23/58 , C12M21/08 , C12M27/02 , C12M27/08 , C12M27/20 , C12M29/06 , C12N5/00 , C12N2527/00
摘要： The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
摘要翻译：本发明涉及具有至少两个叶轮的大规模生物反应器，大规模生物反应器系统以及使用这些生物反应器大规模培养和繁殖哺乳动物细胞的方法。

4. 发明申请

WO2006111387A3 MAMMALIAN EXPRESSION VECTOR COMPRISING THE MCMV PROMOTER AND FIRST INTRON OF HCMV MAJOR IMMEDIATE EARLY GENE 审中-公开
标题翻译：包含MCMV促进剂和HCMV第一内含子的MAMMALIAN表达载体立即生长
公开(公告)号：WO2006111387A3
公开(公告)日：2007-03-29
申请号：PCT/EP2006003641
申请日：2006-04-20
申请人： LONZA BIOLOGICS PLC , GAY ROBERT , KALLMEIER ROBERT , NORMAN ALISON , KALWY STEPHAN
发明人： GAY ROBERT , KALLMEIER ROBERT , NORMAN ALISON , KALWY STEPHAN
IPC分类号： C12N15/09 , C12N5/10 , C12N15/63 , C12N15/85
CPC分类号： C12N15/85 , C12N2800/108 , C12N2830/42 , C12N2840/20
摘要： The invention relates to a mammalian expression vector comprising a murine CMV promoter and the first intron of the major immediate early gene of the human cytomegalovirus, mammalian host cells containing the expression vector and a process for the production of a recombinant protein by using the expression vector.
摘要翻译：本发明涉及包含鼠CMV启动子和人巨细胞病毒的主要即时早期基因的第一内含子的哺乳动物表达载体，含有表达载体的哺乳动物宿主细胞和通过使用表达载体产生重组蛋白的方法。

5. 发明申请

WO2006024497A1 AFFINITY- PLUS ION EXCHANGE- CHROMATOGRAPHY FOR PURIFYING ANTIBODIES 审中-公开
标题翻译：亲和力交换色谱纯化抗体
公开(公告)号：WO2006024497A1
公开(公告)日：2006-03-09
申请号：PCT/EP2005/009343
申请日：2005-08-30
申请人： LONZA BIOLOGICS PLC. , BONNERJEA, Julian , BRAKE, Robert, P. , DAVIS, Mark, Robert , KELLERMAN, Keith , PRENETA, Anna
发明人： BONNERJEA, Julian , BRAKE, Robert, P. , DAVIS, Mark, Robert , KELLERMAN, Keith , PRENETA, Anna
IPC分类号： C07K16/06 , B01D15/26 , B01D15/38 , B01D15/36
CPC分类号： B01D15/1871 , B01D15/362 , B01D15/363 , B01D15/3809 , C07K16/065 , G01N30/461
摘要： A novel method for purifying antibody and other product protein concomittant with removing aggregates made up from single product protein species is devised.
摘要翻译：设计了一种用于纯化与单一产品蛋白质组成的去除聚集体的抗体和其他产品蛋白质相结合的新方法。

6. 发明申请

WO2004009823A1 METHOD OF EXPRESSING RECOMBINANT PROTEIN IN CHO CELLS 审中-公开
标题翻译：在CHO细胞中表达重组蛋白的方法
公开(公告)号：WO2004009823A1
公开(公告)日：2004-01-29
申请号：PCT/EP2003/007946
申请日：2003-07-21
申请人： LONZA BIOLOGICS PLC. , KALLMEIER, Robert , GAY, Robert
发明人： KALLMEIER, Robert , GAY, Robert
IPC分类号： C12N15/85
CPC分类号： C12N15/907 , C12N2800/108 , C12N2830/42 , C12N2840/20
摘要： Method of expressing recombinant protein in CHO cells, by using an expression vector comprising the murine IgG 2A gene locus.
摘要翻译：通过使用包含鼠IgG2A基因座的表达载体在CHO细胞中表达重组蛋白的方法。

7. 发明申请

WO03027300A3 USE OF AMINOGLYCOSIDE RESISTANCE GENE 审中-公开
标题翻译：氨基酸耐药基因的使用
公开(公告)号：WO03027300A3
公开(公告)日：2003-08-21
申请号：PCT/GB0204522
申请日：2002-09-26
申请人： LONZA BIOLOGICS PLC , AL-RUBEAI MOHAMED , PERANI ANGELO , RACHER ANDY , BIRCH JOHN
发明人： AL-RUBEAI MOHAMED , PERANI ANGELO , RACHER ANDY , BIRCH JOHN
IPC分类号： C12N15/09 , C12N5/10 , C12N15/65 , C12N15/85 , C12P21/02 , C12N9/12
CPC分类号： C12N15/65 , C12N15/85 , C12N2800/108 , C12N2830/38 , C12N2840/20 , C12P21/02
摘要： Use of aminoglycoside resistance gene product for achieving high-density growth of animal cells.
摘要翻译：使用氨基糖苷类抗性基因产物实现动物细胞的高密度生长。

8. 发明申请

WO03012449A2 METHOD FOR SELECTING ANTIBODY EXPRESSING CELLS 审中-公开
标题翻译：选择抗体表达细胞的方法
公开(公告)号：WO03012449A2
公开(公告)日：2003-02-13
申请号：PCT/EP0208054
申请日：2002-07-19
申请人： LONZA BIOLOGICS PLC , RACHER ANDY , SINGH RABINDER
发明人： RACHER ANDY , SINGH RABINDER
IPC分类号： G01N33/48 , C12N5/10 , G01N21/78 , G01N33/53 , G01N33/543 , G01N33/569
CPC分类号： G01N33/56966
摘要： A method for selecting high level antibody producing cells, comprising the step of attaching protein A or protein G to a cell surface, thus capturing secreted, soluble antibody. Fluorescence staining of the captured antibody by conventional methods and subsequent FACS analysis allows to select low abundance high producer single cells.
摘要翻译：一种选择产生高水平抗体的细胞的方法，包括将蛋白质A或蛋白质G附着到细胞表面的步骤，从而捕获分泌的可溶性抗体。通过常规方法和随后的FACS分析捕获的抗体的荧光染色允许选择低丰度的高产量单细胞。

9. 发明申请

WO2002099100A3 METHOD OF PRODUCTION OF A PROTEIN IN CELLS WHICH INDUCIBLY EXPRESS THE CELL CYCLE INHIBITOR PROTEIN, P21 审中-公开
公开(公告)号：WO2002099100A3
公开(公告)日：2002-12-12
申请号：PCT/EP2002/006054
申请日：2002-06-03
申请人： LONZA BIOLOGICS PLC , AL-RUBEAI, Mohamed , SHUTTLEWORTH, John,
发明人： AL-RUBEAI, Mohamed , SHUTTLEWORTH, John,
IPC分类号： C07K14/47
摘要： A method for expression of a recombinant protein in a producer cell line comprises the step of co-expressing recombinant p21 or another cell cycle inhibitor protein.

10. 发明申请

WO2017032834A1 MAMMALIAN EXPRESSION SYSTEM 审中-公开
标题翻译： MAMMALIAN表达系统
公开(公告)号：WO2017032834A1
公开(公告)日：2017-03-02
申请号：PCT/EP2016/070083
申请日：2016-08-25
申请人： LONZA BIOLOGICS PLC.
发明人： YOUNG, Robert , BUDGE, James D. , SMALES, C. Mark
IPC分类号： C12N15/85 , C12N15/67
CPC分类号： C12N15/67 , C12N15/85 , C12N2800/108 , C12N2800/40
摘要： The present invention relates to an expression system for the heterologous expression of a nucleic acid sequence of interest in a mammalian cell, the system comprising: (i) a first genetic entity, comprising: a nucleic acid sequence encoding a functional Epstein Barr virus nuclear antigen 1 (EBNA-1), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional EBNA-1; (ii) a second genetic entity, comprising: a nucleic acid sequence encoding a functional nucleoside diphosphate kinase A (NDPK-A), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional NDPK-A; (iii) a third genetic entity, comprising: the nucleic acid sequence of interest being operably linked to regulatory elements that allow for expression of the nucleic acid sequence of interest; and (iv) a four genetic entity, comprising: the Epstein Barr virus OriP sequence or one or more subsequences thereof, wherein the one or more subsequences comprise at least the 'Family of Repeats' DNA-binding site for EBNA-1 and the 'Dyad Symmetry' DNA-binding site for EBNA-1. The present invention also relates to corresponding mammalian host cells and methods for expressing a nucleic acid sequence of interest by means of such expression system.
摘要翻译：本发明涉及用于在哺乳动物细胞中异源表达感兴趣的核酸序列的表达系统，所述系统包含：（i）第一遗传实体，其包含：编码功能性爱泼斯坦巴尔病毒核抗原的核酸序列 1（EBNA-1），所述核酸序列可操作地连接到允许表达编码功能性EBNA-1的核酸序列的调节元件; （ii）第二遗传实体，其包含：编码功能性核苷二磷酸激酶A（NDPK-A）的核酸序列，所述核酸序列可操作地连接于允许表达编码功能性NDPK的核酸序列的调节元件 -一个; （iii）第三遗传实体，其包含：感兴趣的核酸序列可操作地连接到允许表达感兴趣的核酸序列的调节元件; 其包含：爱泼斯坦巴尔病毒OriP序列或其一个或多个子序列，其中所述一个或多个亚序列至少包含EBNA-1的“重复家族DNA”结合位点和“ Dyad Symmetry'DNA结合位点用于EBNA-1。本发明还涉及相应的哺乳动物宿主细胞和通过这种表达系统表达感兴趣的核酸序列的方法。

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