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2. 发明申请

WO9704314A2 DETERMINATION OF AN ANALYTE IN A LIQUID MEDIUM 审中-公开
标题翻译：液体介质中分析物的测定
公开(公告)号：WO9704314A2
公开(公告)日：1997-02-06
申请号：PCT/IL9600049
申请日：1996-07-16
申请人： YISSUM RES DEV CO , WILLNER ITAMAR , LEVI SHLOMO , COHEN YAEL , KATZ EUGENII , DAGAN ARIE
发明人： WILLNER ITAMAR , LEVI SHLOMO , COHEN YAEL , KATZ EUGENII , DAGAN ARIE
IPC分类号： G01N5/02 , G01N33/543 , G01N33/566
CPC分类号： G01N33/54373
摘要： Binding between two members of a recognition pair, e.g. antigen-antibody is determined by utilizing a probe comprising a piezoelectric crystal with electrodes on two opposite faces of the crystal. The crystal carries one or more metal plates which may be the same or different than said electrodes, the metal plates having immobilized thereon a first member of a recognition pair. Binding of a second member of the recognition pair to the first member, or dissociation between the two members and release of the second member from the probe, causes a change of immobilized mass which results in a change to the probe's resonance frequency. Said immobilized members may be immobilized on the surface of said metal plates by means of a linking group, having the following general (I): Z-R -Q wherein Z represents a sulphur-containing moiety which is capable of chemical association with, attachment to or chemisorption onto said metal, R represents a connecting group; Q is a functional group which is capable of forming a covalent bond with a moiety of said first member of the recognition pair. The immobilized member may have or be linked to an isomerizable group which changes its isomerization state as a result of exposure to energy.
摘要翻译：识别对的两个成员之间的绑定，例如通过利用包含晶体的两个相对面上的电极的压电晶体的探针来测定抗原抗体。晶体携带一个或多个金属板，其可以与所述电极相同或不同，金属板固定有识别对的第一构件。识别对的第二个成员与第一个成员的结合，或两个成员之间的解离和第二个成员从探针的释放导致固定的质量的变化，导致探针的共振频率的变化。所述固定化构件可以通过具有以下通式（I）的连接基团固定在所述金属板的表面上：ZR 1 -Q其中Z代表能与硫化物缔合的含硫部分，与所述金属的附着或化学吸附，R 1表示连接基团; Q是能够与识别对的所述第一成员的部分形成共价键的官能团。固定的构件可以具有或连接到由于暴露于能量而改变其异构化状态的异构化基团。

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