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    • 9. 发明申请
    • METHOD FOR RELEASING REDUCING GLYCAN BY AMMONIUM SALT
    • 用盐释放还原糖的方法
    • US20110275108A1
    • 2011-11-10
    • US13058815
    • 2009-08-11
    • Shin-Ichiro NishimuraYoshiaki Miura
    • Shin-Ichiro NishimuraYoshiaki Miura
    • C12Q1/02C07C271/02C07H1/00C01C1/26G01N33/50
    • G01N33/66C07C271/02Y10T436/143333
    • An objective of the present invention is to provide a glycan releasing method which can be applied to construction of a system for automation of glycan analysis, and particularly a glycan releasing method capable of analyzing an O-linked glycan. The objective could be achieved as a result of finding that the pH is lowered by using an ammonium salt or ammonium ion in the absence of concentrated aqueous ammonia, not using concentrated aqueous ammonia, thus drastically avoiding an undesired side reaction such as a peeling reaction or the like. Therefore, the present invention provides a method of producing an O-linked glycan from a glycan-binding substance having the O-linked glycan, the method includes the steps of (A) bringing an ammonium salt or ammonium ion into contact with the glycan-binding substance in the absence of concentrated aqueous ammonia (for example, under the conditions of the pH of about 7 or higher and lower than about 11); (B) neutralizing the reaction solution obtained in step (A); and (C) collecting the released glycan.
    • 本发明的目的是提供可用于构建聚糖分析自动化系统的聚糖释放方法,特别是能够分析O-连接的聚糖的聚糖释放方法。 可以通过在不存在浓氨水的情况下使用铵盐或铵离子来降低pH而不使用浓氨水的结果,从而大大地避免了不期望的副反应如剥离反应或 类似。 因此,本发明提供了由具有O-连接聚糖的聚糖结合物质制备O-连接聚糖的方法,该方法包括以下步骤:(A)使铵盐或铵离子与聚糖 - 在不存在浓氨水的情况下(例如,在约7以上且低于约11的pH的条件下)结合物质; (B)中和步骤(A)中获得的反应溶液; 和(C)收集所释放的聚糖。