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1. 发明授权

US07374897B2 Enzyme cycling based assays for alpha-methylacyl-CoA racemase 失效
标题翻译： α-甲酰基-CoA消旋酶的酶循环测定
公开(公告)号：US07374897B2
公开(公告)日：2008-05-20
申请号：US10966983
申请日：2004-10-15
申请人： Chong-Sheng Yuan
发明人： Chong-Sheng Yuan
IPC分类号： C12Q1/15
CPC分类号： C12Q1/533 , G01N33/574
摘要： The present invention provides a method for assaying alpha-methylacyl-CoA racemase activity. In the assay, a sample containing an alpha-methylacyl-CoA racemase or suspected of containing an alpha-methylacyl-CoA racemase is contacted with (2R)-pristanoyl-CoA. If alpha-methylacyl-CoA racemase is present in the sample, (2R)-pristanoyl-CoA is converted into (2S)-pristanoyl-CoA. The method then utilizes a cycling reaction system between (2S)-pristanoyl-CoA and trans-2,3-dehydropristanoyl-CoA to generate a detectable signal that corresponds to the alpha-methylacyl-CoA racemase activity. Kits for assaying alpha-methylacyl-CoA racemase based on the same principle are also provided.
摘要翻译：本发明提供了测定α-甲酰基-CoA消旋酶活性的方法。在该测定中，含有α-甲酰基-CoA消旋酶或怀疑含有α-甲酰基-CoA消旋酶的样品与（2R） - 丙酰辅酶A接触。如果样品中存在α-甲酰基-CoA消旋酶，则将（2R） - 丙酰辅酶A转化为（2S） - 丙酰辅酶A。该方法然后利用（2S） - 丙酰基-CoA和反式-2,3-脱氢丙酰辅酶A之间的循环反应系统产生对应于α-甲酰基-CoA消旋酶活性的可检测信号。还提供了基于相同原理测定α-甲基酰基-CoA消旋酶的试剂盒。

2. 发明授权

US06927037B2 Ion-exchange resin/enzyme activity assay 失效
标题翻译：离子交换树脂/酶活性测定
公开(公告)号：US06927037B2
公开(公告)日：2005-08-09
申请号：US09888008
申请日：2001-06-22
申请人： Thomas P. Kasten , Mark G. Currie , William M. Moore , Kay Broschat
发明人： Thomas P. Kasten , Mark G. Currie , William M. Moore , Kay Broschat
IPC分类号： C12Q1/00 , C12Q1/48 , C12Q1/15
CPC分类号： C12Q1/00 , C12Q1/485
摘要： The present invention relates to a rapid high-throughput ion-exchange resin assay for determining enzyme activity. This novel assay uses a radiometric technique which separates the radioactive substrate from the product (or the radioactive product from the substrate) by exploiting the differences in the net charges of the molecules using ion-exchange resin. This assay is useful, for example, for studies of enzyme kinetics, the identification of functional sites in the enzyme, and in the automated screening of compound libraries for pharmaceutical drug development.
摘要翻译：本发明涉及用于测定酶活性的快速高通量离子交换树脂测定法。这种新型测定法使用辐射技术，其通过使用离子交换树脂利用分子的净电荷差异将放射性底物与产物（或放射性产物与底物）分离。该测定法可用于例如酶动力学的研究，酶的功能位点的鉴定以及用于药物开发的化合物文库的自动筛选。

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