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1. 发明授权

US06300117B1 Proteases from gram-positive organisms 有权
标题翻译：来自革兰氏阳性菌的蛋白酶
公开(公告)号：US06300117B1
公开(公告)日：2001-10-09
申请号：US09308375
申请日：1999-05-14
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N954
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , C12N15/75 , D06M16/003
摘要： The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.
摘要翻译：本发明涉及革兰阳性微生物中新型金属蛋白酶（MP）的鉴定。本发明提供芽孢杆菌（MP）的核酸和氨基酸序列。本发明还提供具有编码MP的基因的部分或全部的突变或缺失的宿主细胞。本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。本发明还提供了包含本发明的MP的清洁组合物。

2. 发明授权

US06287841B1 High alkaline serine protease 失效
标题翻译：高碱性丝氨酸蛋白酶
公开(公告)号：US06287841B1
公开(公告)日：2001-09-11
申请号：US08090207
申请日：1993-07-27
申请人： Leonardus Johannes Sofie Marie Mulleners , Onno Misset , Jan Metske Van Der Laan , Franciscus Josephus Cornelius Van Gastel , Cornelis Petrus Broekhuizen , Erik Jan Baas
发明人： Leonardus Johannes Sofie Marie Mulleners , Onno Misset , Jan Metske Van Der Laan , Franciscus Josephus Cornelius Van Gastel , Cornelis Petrus Broekhuizen , Erik Jan Baas
IPC分类号： C12N954
CPC分类号： C11D3/386 , C12N9/54
摘要： New PB92 or Subtilisin 309 mutant serine proteases are provided having specific mutations, resulting in an surprisingly better wash performance or in an improved storage stability with at similar or even better wash performance. These PB92 or Subtilisin 309 mutants include mutations at positions 60, 87, 97, 99, 102, 116, 117, 126, 127, 128, 130, 133, 134, 154, 156, 158, 159, 160, 164, 169, 175, 180, 182, 193, 197, 198, 203, 211, and 216. The new proteases, therefore, are very suitable for use in various types of detergents, whether or not in conjunction with other enzymes, for example amylases, cellulases and lipases. Preferred embodiments are the PB92 and Subtilisin 309 mutants having a mutation at position 102 and in particular those having at least one further mutation.
摘要翻译：提供新的PB92或枯草杆菌蛋白酶309突变丝氨酸蛋白酶具有特异性突变，导致惊人的更好的洗涤性能或改善的储存稳定性，同样或甚至更好的洗涤性能。这些PB92或枯草杆菌蛋白酶309突变体包括位置60,87,97,99,102,116,117,126,127,128,130,133,134,154,156,158,159,160,164,169的突变，因此，新的蛋白酶非常适合用于各种类型的洗涤剂，无论是否与其它酶联合使用，例如淀粉酶，纤维素酶和脂肪酶。优选的实施方案是在102位具有突变的PB92和枯草杆菌蛋白酶309突变体，特别是具有至少一个其它突变的突变体。

3. 发明授权

US06586223B1 Subtilisin protease variants having amino acid substitutions in defined epitope regions 失效
标题翻译：枯草杆菌蛋白酶变体在定义的表位区域具有氨基酸取代
公开(公告)号：US06586223B1
公开(公告)日：2003-07-01
申请号：US09618573
申请日：2000-07-18
申请人： Elizabeth Ellen Sikorski , Donn Nelton Rubingh
发明人： Elizabeth Ellen Sikorski , Donn Nelton Rubingh
IPC分类号： C12N954
CPC分类号： C12N9/54 , C11D3/386
摘要： The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a substitution of one or more epitope regions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.
摘要翻译：本发明涉及相对于其相应的野生型蛋白酶具有降低的免疫原性的枯草杆菌蛋白酶样蛋白酶的变体。更具体地，本发明涉及具有野生型氨基酸序列的修饰的氨基酸序列的变体，其中修饰的氨基酸序列包含一个或多个表位区域的取代。本发明还涉及编码这种变体的突变基因和包含这些变体的清洁和个人护理组合物。

4. 发明授权

US06218165B1 Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins 有权
标题翻译：人类具有较低过敏反应的突变蛋白质以及用于构建，鉴定和产生此类蛋白质的方法
公开(公告)号：US06218165B1
公开(公告)日：2001-04-17
申请号：US09255502
申请日：1999-02-23
申请人： David A. Estell , Fiona A. Harding
发明人： David A. Estell , Fiona A. Harding
IPC分类号： C12N954
CPC分类号： G01N33/6878 , C12N9/54 , G01N33/505
摘要： The present invention relates to a novel improved protein mutant which produces low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein.
摘要翻译：本发明涉及与该突变体的亲本相比，在人类中产生低变应原反应的新型改良蛋白质突变体。具体地，本发明包括中和或降低T细胞识别表位的能力，从而防止个体对蛋白质的致敏。

5. 发明授权

US06190904B1 High-alkaline protease and its use arginine-substituted subtilisin composition and use 失效
标题翻译：高碱性蛋白酶及其使用精氨酸取代枯草杆菌蛋白酶的组成和用途
公开(公告)号：US06190904B1
公开(公告)日：2001-02-20
申请号：US08716293
申请日：1996-12-18
申请人： Antoine Amory , André Clippe , Gerhard Konieczny-Janda , Hubert Herrmann , Karsten Börner
发明人： Antoine Amory , André Clippe , Gerhard Konieczny-Janda , Hubert Herrmann , Karsten Börner
IPC分类号： C12N954
CPC分类号： C12N9/54 , C11D3/386 , C11D3/39
摘要： The invention relates to a novel high-alkaline protease, its use in the industrial or domestic fields and also compositions for said uses containing this protease. The protease of the invention is a selected triple mutant derived from certain precursor proteases from the subtilisin group, especially of the “subtilisin 309” type.
摘要翻译：本发明涉及一种新型高碱性蛋白酶，其在工业或家庭领域中的应用以及含有该蛋白酶的所述用途的组合物。本发明的蛋白酶是来自枯草杆菌蛋白酶组特别是“枯草杆菌蛋白酶309”型的某些前体蛋白酶的选择的三重突变体。

6. 发明授权

US06727085B2 Subtilase variants having an improved wash performance on egg stains 有权
标题翻译：具有改善的蛋渍洗涤性能的脱丝酶变体
公开(公告)号：US06727085B2
公开(公告)日：2004-04-27
申请号：US09736116
申请日：2000-12-13
申请人： Tina Sejersgård Fanø , Frank Mikkelsen
发明人： Tina Sejersgård Fanø , Frank Mikkelsen
IPC分类号： C12N954
CPC分类号： C12N9/54 , C11D3/38636
摘要： The present invention relates to the use of a subtilase variant for removal of egg stains from laundry or from hard surfaces, where the subtilase variant comprises at least one additional amino acid residue in the active site loop (b) region from position 95 to 103 (BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, including automatic dishwash compositions. The present invention also relates to novel subtilase variants, to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, the present invention relates to cleaning and detergent compositions comprising the variants of the invention.
摘要翻译：本发明涉及用于从衣物或硬质表面除去蛋渍的枯草芽孢杆菌变体的用途，其中所述枯草芽孢杆菌变体在95至103位的活性位点环（b）区域中包含至少一个另外的氨基酸残基（ BASBPN编号）。这些枯草芽孢杆菌变体在用于例如蛋白质时在蛋渍上表现出优异或改善的洗涤性能是有用的。清洁或洗涤剂组合物，包括自动洗碗组合物。本发明还涉及编码变体，表达载体，宿主细胞的分离的DNA序列以及用于生产和使用本发明的变体的方法的新型脱羧酶变体。此外，本发明涉及包含本发明变体的清洁和洗涤剂组合物。

7. 发明授权

US06673590B1 Multiply-substituted protease variants with altered net charge for use in detergents 有权
公开(公告)号：US06673590B1
公开(公告)日：2004-01-06
申请号：US09177353
申请日：1998-10-23
申请人： Ayrookaran .J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
发明人： Ayrookaran .J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
IPC分类号： C12N954
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.

8. 发明授权

US06586224B1 Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions 失效
标题翻译：枯草杆菌蛋白酶变体在确定的表位区域具有氨基酸缺失和取代
公开(公告)号：US06586224B1
公开(公告)日：2003-07-01
申请号：US09618845
申请日：2000-07-18
申请人： Donn Nelton Rubingh , Elizabeth Ellen Sikorski
发明人： Donn Nelton Rubingh , Elizabeth Ellen Sikorski
IPC分类号： C12N954
CPC分类号： C12N9/54 , C11D3/386
摘要： The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. The present invention further relates to such variants additionally having one or more amino acid substitutions in one or more epitope regions or additionally having one or more stabilizing substitutions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.
摘要翻译：本发明涉及相对于其相应的野生型蛋白酶具有降低的免疫原性的枯草杆菌蛋白酶样蛋白酶的变体。本发明还涉及另外在一个或多个表位区域中另外具有一个或多个氨基酸取代或另外具有一个或多个稳定取代的这些变体。本发明还涉及编码这种变体的突变基因和包含这些变体的清洁和个人护理组合物。

9. 发明授权

US06190900B1 Subtilase variants 失效
标题翻译： Subtilase变体
公开(公告)号：US06190900B1
公开(公告)日：2001-02-20
申请号：US09120577
申请日：1998-07-22
申请人： Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
发明人： Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
IPC分类号： C12N954
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
摘要翻译：本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。与其野生型亲本酶相比，酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。

10. 发明授权

US06780629B2 Subtilase enzymes 失效
标题翻译：分泌酶
公开(公告)号：US06780629B2
公开(公告)日：2004-08-24
申请号：US10243576
申请日：2002-09-13
申请人： Peter Kamp Hansen , Peter Bauditz , Frank Mikkelsen , Kim Vilbour Andersen , Carsten Andersen , Mads Norregaard-Madsen
发明人： Peter Kamp Hansen , Peter Bauditz , Frank Mikkelsen , Kim Vilbour Andersen , Carsten Andersen , Mads Norregaard-Madsen
IPC分类号： C12N954
CPC分类号： C12Y304/21062 , C11D3/386 , C12N9/54 , C12N9/58
摘要： The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (c) region from positions 125 to 132. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.
摘要翻译：本发明涉及在位置125至132的活性位点环（c）区域中具有另外的氨基酸的I-S1和I-S2亚基的脱羧酶。本发明的变体脱水酶表现出改进的洗涤性能在洗涤剂中与其母体酶相比。

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