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1. 发明申请

US20020194642A1 Heat stable mutants of starch biosynthesis enzymes 失效
标题翻译：淀粉生物合成酶的热稳定突变体
公开(公告)号：US20020194642A1
公开(公告)日：2002-12-19
申请号：US10079478
申请日：2002-02-19
发明人： L. Curtis Hannah , Thomas W. Greene
IPC分类号： A01H005/00 , C07H021/04 , C12N009/44 , C12P021/02 , C12N005/04
CPC分类号： C12N9/1241 , C12N9/1051 , C12N15/8245 , C12N15/8271 , C12Y204/01021 , C12Y207/07027
摘要： The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
摘要翻译：本发明涉及编码具有增加的热稳定性的酶的新型突变体多核苷酸分子。当在植物中表达时，这些多核苷酸导致在热胁迫条件下生长的植物中的产量增加。本发明的多核苷酸分子编码玉米胚乳ADP葡萄糖焦磷酸化酶（AGP）和可溶性淀粉合成酶（SSS）酶活性。繁殖含有突变体多核苷酸或转化了突变多核苷酸并表达多核苷酸编码的多肽的植物和植物组织也被本发明考虑。本发明还涉及分离本发明范围内考虑的多核苷酸和多肽的方法。还提供了在热胁迫条件下生长的植物中提高产量的方法。

2. 发明申请

US20040171023A1 Utilization of starch for biological production by fermentation 有权
标题翻译：利用淀粉进行发酵生物生产
公开(公告)号：US20040171023A1
公开(公告)日：2004-09-02
申请号：US10648152
申请日：2003-08-25
发明人： Perry G. Caimi , Mario W. Chen , Vasantha Nigarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
IPC分类号： C12Q001/68 , C07H021/04 , C12N009/44 , C12N009/24
CPC分类号： C12P7/20 , C07K2319/00 , C12N9/2451 , C12N9/246 , C12P7/18 , C12Y302/01068 , C12Y302/01133
摘要： This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant E. coli containing a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.
摘要翻译：本发明涉及一种在发酵过程中利用少量纯化淀粉的方法。一个实例是含有外源细胞外异淀粉酶活性的重组大肠杆菌，其能够利用在发酵中含有（1,6）键（包括但不限于异麦芽糖和panose）的小寡聚物以产生有用的产物。通过降低底物碳水化合物的成本，本发明可用于大规模工业生物发酵。

3. 发明申请

US20040048247A1 Pullulanase variants and methods for preparing such variants with predetermined properties 审中-公开
标题翻译：支链淀粉酶变体和用于制备具有预定性质的这种变体的方法
公开(公告)号：US20040048247A1
公开(公告)日：2004-03-11
申请号：US10069040
申请日：2002-02-19
发明人： Allan Svendsen , Carsten Andersen , Torben Vedel Borchert
IPC分类号： C12Q001/68 , G06G007/48 , G06G007/58 , C12N009/44 , C07H021/04
CPC分类号： C12N9/2457 , C12N9/246 , C12Y302/01041 , C12Y302/01068
摘要： The present invention relates to a method for producing a variant of a parent pullulanase, the variant having at least one altered property as compared to the parent pullulanase. The invention also relates to pullulanase variants and to the use of pullulanase variants of the invention for use in particular starch conversion processes.
摘要翻译：本发明涉及一种生产亲本支链淀粉酶变异体的方法，该变体与亲本支链淀粉酶相比具有至少一种改变的性质。本发明还涉及支链淀粉酶变体和本发明的支链淀粉酶变体在特定淀粉转化方法中的用途。

4. 发明申请

US20030013180A1 MODIFIED FORMS OF PULLULANASE 有权
标题翻译： PULLULANASE的改良形式
公开(公告)号：US20030013180A1
公开(公告)日：2003-01-16
申请号：US09262126
申请日：1999-03-03
发明人： BRIAN S. MILLER , JAYARAMA K. SHETTY
IPC分类号： C12P019/16 , C12N009/44 , C07H021/04 , C12P021/02 , C12N005/06
CPC分类号： C12N9/2457 , C12N9/2451 , C12N9/246 , C12Y302/01041 , C12Y302/01068
摘要： The present invention relates to modified pullulanases useful in the starch industry. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for the saccharification of starch comprising the use of the enzymatic compositions.
摘要翻译：本发明涉及可用于淀粉工业的改性支链淀粉酶。本发明提供了生产改性的支链淀粉酶的方法，包含改性的支链淀粉酶的酶组合物和包含使用酶组合物的淀粉糖化方法。

5. 发明申请

US20020002713A1 Starch branching enzyme IIb 审中-公开
标题翻译：淀粉分支酶IIb
公开(公告)号：US20020002713A1
公开(公告)日：2002-01-03
申请号：US09792127
申请日：2001-02-23
发明人： Stephen M. Allen , Diane M. Beckles , Karlene H. Butler , Richard W. Pearlstein
IPC分类号： A01H005/00 , C07H021/04 , C12N009/44 , C12N005/04
CPC分类号： C12N9/107 , C12N15/8245
摘要： This invention relates to an isolated nucleic acid fragment encoding a starch branching enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the starch branching enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the starch branching enzyme in a transformed host cell.
摘要翻译：本发明涉及编码淀粉分支酶的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分淀粉分支酶的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变的淀粉分支酶水平。

6. 发明申请

US20030190738A1 Starch debranching enzymes 失效
公开(公告)号：US20030190738A1
公开(公告)日：2003-10-09
申请号：US10375720
申请日：2003-02-26
申请人： Novozymes A/S
发明人： Henrik Bisgard-Frantzen , Allan Svendsen
IPC分类号： C12P019/04 , C12P019/02 , C12N009/44 , C12P021/02 , C12N005/06
CPC分类号： C12N9/2457 , C12N9/2451 , C12N9/246 , C12P19/16 , C12Y302/01041 , C12Y302/01068
摘要： The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.

7. 发明申请

US20030054539A1 Endoglucanases 失效
标题翻译：内切葡聚糖酶
公开(公告)号：US20030054539A1
公开(公告)日：2003-03-20
申请号：US10007521
申请日：2001-12-10
申请人： Novozymes A/S
发明人： Martin Schulein , Hanne Dela , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
IPC分类号： C12N009/44 , D06M016/00
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence 1 (SEQ ID NO:79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa 1nullnullnull2nullnullnull3nullnullnull4nullnullnull5nullnullnull6nullnullnull7nullnullnull8nullnullnull9 Xaa Cys Xaa Trp Xaa 10nullnull11nullnull12nullnull13nullnull14 and a second amino acid sequence having the following sequence 2 Trp Cys Cys Xaa Cys (SEQ ID NO:80) 1nullnullnull2nullnullnull3nullnullnull4nullnullnull5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译：本发明涉及酶制剂，其基本上由具有纤维素分解活性的酶组成，并且包含具有以下序列的第一氨基酸序列和具有以下序列的第二个氨基酸序列，其中在第一序列的3位是氨基酸是Trp，Tyr或Phe; 在第一序列的位置4处，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 在第一序列的第9,10,12和14位，第2个序列的第4位，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列，（i）当第12位的氨基残基为Ser时，第14位的氨基酸残基不为Ser，（ii）当第12位的氨基残基为Gly时，第14位的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

8. 发明申请

US20020081670A1 Starch debranching enzymes 审中-公开
标题翻译：淀粉脱支酶
公开(公告)号：US20020081670A1
公开(公告)日：2002-06-27
申请号：US09833435
申请日：2001-04-12
申请人： Novozymes A/S
发明人： Henrik Bisgard-Frantzen , Allan Svendsen
IPC分类号： C12P019/04 , C12N009/44 , C12N015/00
CPC分类号： C12N9/2457 , C12N9/2451 , C12N9/246 , C12P19/16 , C12Y302/01041 , C12Y302/01068
摘要： The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.
摘要翻译：本发明涉及母体淀粉脱支酶的遗传工程改造，即支链淀粉酶或淀粉酶，所述酶变体在与亲本酶相比在4-6的范围内具有改善的热稳定性和/或增加的活性对于支链淀粉和/或糖原，与制备具有改进的热稳定性和/或改变的底物特异性的淀粉脱支酶变体的方法以及使用至少一种这样的酶变体将淀粉转化为一种或多种糖的方法。

9. 发明申请

US20040161837A1 Methods of purifying human acid alpha-glucosidase 审中-公开
标题翻译：纯化人类α-葡萄糖苷酶的方法
公开(公告)号：US20040161837A1
公开(公告)日：2004-08-19
申请号：US10777644
申请日：2004-02-13
发明人： Arnold J. Reuser , Ans T. van der Ploeg
IPC分类号： C12N009/44
CPC分类号： C12N9/2408 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2217/075 , A01K2227/105 , A01K2227/107 , A01K2267/01 , A23C9/20 , A23C2230/05 , A61K38/00 , C12N15/8509
摘要： The invention provides methods of purifying lysosomal proteins, pharmaceutical compositions for use in enzyme replacement therapy, and methods of treating Pompe's disease using purified human acid alpha glucosidase.
摘要翻译：本发明提供了纯化溶酶体蛋白的方法，用于酶替代疗法的药物组合物，以及使用纯化的人酸性α糖苷酶处理庞培病的方法。

10. 发明申请

US20040082028A1 Pullulanase variants and methods for preparing such variants with predetermined properties 失效
标题翻译：支链淀粉酶变体和用于制备具有预定性质的这种变体的方法
公开(公告)号：US20040082028A1
公开(公告)日：2004-04-29
申请号：US09996024
申请日：2001-11-16
申请人： Novozymes A/S
发明人： Allan Svendsen
IPC分类号： C12P021/02 , C12Q001/68 , C12Q001/34 , G06F019/00 , G01N033/48 , G01N033/50 , C12N009/44 , C12N005/06
CPC分类号： C12N9/2457 , C12N9/246 , C12Q1/34 , C12Y302/01041 , C12Y302/01068 , G01N2333/918
摘要： The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostabilty, altered substrate specificity, increased specific activity or altered cleavage pattern. The present invention also relates to methods of making pullulanase variants having at least one altered property based on the three-dimensional structure of a parent pullulanase.
摘要翻译：本发明涉及支链淀粉酶变体，其中所述变体具有改善的性质，例如改变的pH最佳，改善的热稳定性，改变的底物特异性，增加的比活性或改变的切割模式。本发明还涉及基于亲本支链淀粉酶的三维结构制备具有至少一种改变的属性的支链淀粉酶变体的方法。

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