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1. 发明申请

US20040253676A1 Method for producing sweetners and alcohol 审中-公开
标题翻译：生产甜酒和酒精的方法
公开(公告)号：US20040253676A1
公开(公告)日：2004-12-16
申请号：US10779418
申请日：2004-02-12
申请人： Novozymes A/S
发明人： Henrik Bisgard-Frantzen , Allan Svendsen , Torben Vedel Borchert
IPC分类号： C12P021/02 , C12N009/32 , C07H021/04 , C11D003/386
CPC分类号： C11D3/38618 , C11D3/38609 , C11D3/38681 , C12N9/2417 , C12Y302/01001 , D06L1/14
摘要： The present invention relates to variants of a parent null-amylase, which parent null-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an null-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an null-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent null-amylase has been deleted; and/or (b) at least one amino acid residue of the parent null-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent null-amylase; the variant having null-amylase activity and exhibiting at least one of the following properties relative to the parent null-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2null dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.
摘要翻译：本发明涉及母体α-淀粉酶的变体，其母体α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶表现出以下特性中的至少一个：增加的热稳定性; 提高氧化稳定性; 并减少Ca 2+依赖性; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。

2. 发明申请

US20040009570A1 Alkaline lipase from Vibrio metschnikovii RH530 N-4-8 and nucleotide sequence encoding the same 有权
标题翻译：来自Vibrio metschnikovii RH530 N-4-8的碱性脂肪酶及其编码的核苷酸序列
公开(公告)号：US20040009570A1
公开(公告)日：2004-01-15
申请号：US10603260
申请日：2003-06-24
发明人： Ghee Hong Jin , Sung Hoo Jhon , Hyun Hwan Lee , Hyune Mo Rho
IPC分类号： C11D003/386 , C07H021/04 , C12N009/20 , C12N001/21 , C12P021/02 , C12N015/74
CPC分类号： C12N9/20 , C11D3/38627
摘要： An alkaline lipase isolated from Vibrio metschnikovii RH530 and a polynucleotide sequence encoding the same are provided. The isolated alkaline lipase has an amino acid sequence of SEQ ID NO: 5 and the polynucleotide having a base sequence of SEQ ID NO: 4 encodes the alkaline lipase. The isolated alkaline lipase exhibits an optimal activity at a high pH level, that is, at pH 10null11, and has very high ratio of residual enzyme activity and high compatibility with a surfactant, so that it can be suitably used as an enzyme for a laundry detergent.
摘要翻译：提供了从弧菌弧菌RH530分离的碱性脂肪酶和编码该碱性脂肪酶的多核苷酸序列。分离的碱性脂肪酶具有SEQ ID NO：5的氨基酸序列，并且具有SEQ ID NO：4的碱基序列的多核苷酸编码碱性脂肪酶。分离的碱性脂肪酶在高pH值，即pH 10〜11时表现出最佳活性，并且具有非常高的残留酶活性比率和与表面活性剂的高相容性，因此其可以适合用作酶洗衣粉。

3. 发明申请

US20030176304A1 Protease variants and compositions 失效
标题翻译：蛋白酶变体和组成
公开(公告)号：US20030176304A1
公开(公告)日：2003-09-18
申请号：US10336324
申请日：2003-01-03
申请人： Novozymes A/S
发明人： Peter Kamp Hansen , Peter Bauditz , Frank Mikkelsen , Kim Vilbour Andersen
IPC分类号： C12N009/56 , C07H021/04 , C12P021/02 , C12N001/21 , C11D003/386 , C12N015/74
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.
摘要翻译：本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。与其野生型亲本酶相比，酶在任何洗涤剂中表现出改善的洗涤性能。

4. 发明申请

US20030175933A1 Useful mutations of bacterial alkaline protease 失效
标题翻译：细菌碱性蛋白酶有用的突变
公开(公告)号：US20030175933A1
公开(公告)日：2003-09-18
申请号：US10313853
申请日：2002-12-06
申请人： Novozymes A/S
发明人： Sven Hastrup , Sven Branner , Fanny Norris , Steffen Bjorn Petersen , Leif Norskov-Lauridsen , Villy Johannes Jensen , Dorrit Aaslyng
IPC分类号： C07H021/04 , C12N009/56 , C12P021/02 , C12N001/21 , C11D003/386 , C12N015/74
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.
摘要翻译：本发明涉及导致枯草杆菌蛋白酶的化学特性变化的枯草杆菌蛋白酶基因的突变。枯草杆菌蛋白酶基因的特异性核酸的突变导致氨基酸取代，从而导致酶功能的改变。这些突变体酶中的一些显示出对于工业应用有利的物理性质，特别是在洗涤剂工业中，提供对氧化更稳定的枯草杆菌蛋白酶，具有更大的蛋白酶活性，并且表现出改进的可洗性。

5. 发明申请

US20030119006A1 BGL4 beta-glucosidase and nucleic acids encoding the same 有权
标题翻译： BGL4β-葡糖苷酶和编码相同的核酸
公开(公告)号：US20030119006A1
公开(公告)日：2003-06-26
申请号：US10027000
申请日：2001-12-18
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
IPC分类号： C12Q001/68 , C07H021/04 , C12P007/06 , C12N009/42 , C12N001/16 , C11D003/386 , C12P021/02 , C12N015/74
CPC分类号： C12N9/2445 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a novel null-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.
摘要翻译：本发明提供了一种命名为bgl4的新型β-葡糖苷酶核酸序列和相应的BGL4氨基酸序列。本发明还提供了包含编码BGL4的核酸序列，重组BGL4蛋白的表达载体和宿主细胞及其制备方法。

6. 发明申请

US20030078179A1 Cleaning compositions containing multiply-substituted protease variants 有权
公开(公告)号：US20030078179A1
公开(公告)日：2003-04-24
申请号：US10047689
申请日：2002-01-15
申请人： The Procter & Gamble Company
发明人： Chanchal Kumar Ghosh , Andre Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis JR. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
IPC分类号： C11D003/00 , C11D003/386
CPC分类号： C11D3/386
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a subtitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.

7. 发明申请

US20030078177A1 Proteases from gram positive organisms 有权
标题翻译：来自革兰氏阳性菌的蛋白酶
公开(公告)号：US20030078177A1
公开(公告)日：2003-04-24
申请号：US10285074
申请日：2002-10-31
发明人： David A. Estell
IPC分类号： C11D003/386 , C07H021/04 , C12N009/56 , C12P021/02 , C12N001/21 , C12N015/74
CPC分类号： C12N9/54 , C11D3/38663 , C12N9/80 , C12N15/75
摘要： The present invention relates to the identification of a novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
摘要翻译：本发明涉及革兰氏阳性微生物中新型金属蛋白酶的鉴定。本发明提供了金属蛋白酶的核酸和氨基酸序列。本发明还提供具有编码金属蛋白酶的基因的部分或全部的突变或缺失的宿主细胞。本发明提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。本发明还提供了用于处理包括本发明的金属蛋白酶的织物的清洁组合物，动物饲料和组合物。

8. 发明申请

US20030003561A1 Lipolytic enzyme 失效
标题翻译：脂解酶
公开(公告)号：US20030003561A1
公开(公告)日：2003-01-02
申请号：US09931401
申请日：2001-08-16
申请人： Novozymes A/S
发明人： Jesper Vind
IPC分类号： C12N009/20 , A21D002/00 , A21D008/02 , C07H021/04 , C12P021/02 , C12N001/21 , C11D003/386 , C12N015/74
CPC分类号： C11D3/38627 , A21D8/042 , C12N9/20
摘要： The present invention is directed to phospholipase from Fusarium solani (MUCL38667) and analogues thereof, nucleic acid constructs, recombinant expression vectors, and recombinant host comprising the nucleic acid sequences encoding the phospholipase from Fusarium solani (MUCL38667) and analogues thereof, methods of making same, and method of using the phosopholipases of the present invention in baking methods, baking compositions and detergent compositions.
摘要翻译：本发明涉及来自茄科镰刀菌（MUCL38667）的磷脂酶及其类似物，核酸构建体，重组表达载体和重组宿主，其包含编码来自茄科（MUCL38667）的磷脂酶的核酸序列及其类似物，其制备方法以及本发明的磷脂酰胆碱在烘烤方法，烘烤组合物和洗涤剂组合物中的使用方法。

9. 发明申请

US20020128166A1 Detergents comprising cellulases 失效
标题翻译：含有纤维素酶的洗涤剂
公开(公告)号：US20020128166A1
公开(公告)日：2002-09-12
申请号：US09863547
申请日：2001-05-23
发明人： Hermanus Bernardus Maria Lenting , Rudolf Franciscus Wilhelmus Cornelis Van Beckhoven , Karl-Heinz Maurer , Beatrix Kottwitz , Albrecht Weiss , Pieter Van Solingen
IPC分类号： C11D003/00 , C11D003/386
CPC分类号： C12N9/2437 , C12Y302/01004 , D06M2200/20
摘要： Laundry detergent compositions containing one or more cellulases having a ratio of tensile strength loss to antipilling properties of less than 1. The cellulases may be obtained from CBS 9.93 or CBS 670.93.
摘要翻译：含有一种或多种纤维素酶的洗涤洗涤剂组合物，其拉伸强度损失与抗起始性能的比小于1.纤维素酶可以从CBS 9.93或CBS 670.93获得。

10. 发明申请

US20020082181A1 Cleaning, laundering or treating compositions containing cross-linked hydrolase crystals 审中-公开
标题翻译：清洗，洗涤或处理含有交联水解酶晶体的组合物
公开(公告)号：US20020082181A1
公开(公告)日：2002-06-27
申请号：US10002371
申请日：2001-11-15
申请人： THE CLOROX COMPANY
发明人： Vickie L . Humphrey , Kevin L. Stellner
IPC分类号： C11D003/386 , C11D007/18 , C11D003/00 , C11D009/42
CPC分类号： C11D3/38663 , C11D1/02 , C11D1/38 , C11D1/66 , C11D1/88 , C11D3/3947
摘要： The present invention provides a product and method for cleaning or laundering or treatment of fabrics or surfaces, with a liquid cleaning, laundering or treating composition having improved enzyme stability in the presence of an oxidant comprising a) at least one cross-linked hydrolase crystal, or mixture of hydrolase crystals; b) an oxidant, or source of hydrogen peroxide; and c) at least one cleaning, laundering or treating additive, other than an oxidant, wherein the improved stability is due to one or more of the following: (i) where c) includes a surfactant, maintaining the upper limit thereof to no greater than 10% by weight of the composition; (ii) maintaining the pH of the liquid composition between 5 and 8; and (iii) where c) includes surfactants, no more than 20% of the total surfactants is anionic. The use of the cross-linked hydrolase crystals results in compositions which have surprisingly proficient retained hydrolytic stability in the presence of such oxidant versus a commercially available non-cross-linked enzyme.
摘要翻译：本发明提供了一种用于清洁或洗涤或洗涤织物或表面的产品和方法，其具有在氧化剂存在下具有改善的酶稳定性的液体清洁，洗涤或处理组合物，其包含：a）至少一种交联的水解酶晶体，或水解酶晶体的混合物; b）氧化剂或过氧化氢源; 和c）除了氧化剂之外的至少一种清洁，洗涤或处理添加剂，其中改进的稳定性是由于以下一种或多种：（i）其中c）包括表面活性剂，将其上限保持不大于超过组合物重量的10％; （ii）保持液体组合物的pH在5和8之间; 和（iii）其中c）包括表面活性剂，不超过20％的总表面活性剂是阴离子的。使用交联的水解酶晶体导致在这种氧化剂存在下，与商业上可获得的非交联酶相比，令人惊奇地保持水解稳定性的组合物。

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