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    • 1. 发明授权
    • Method for quantifying cholesterol in high density lipoprotein
    • 高密度脂蛋白胆固醇定量方法
    • US06893832B2
    • 2005-05-17
    • US10190793
    • 2002-07-09
    • Hiroshi MatsuiYasuki ItoShuichi OharaAkira Fujiwara
    • Hiroshi MatsuiYasuki ItoShuichi OharaAkira Fujiwara
    • C12Q1/60C01N33/92
    • C12Q1/60Y10S436/824
    • Disclosed is a method for quantifying cholesterol in HDL, which does not require complex fragmentation and separation operations, and by which the HDL cholesterol in test samples containing HDL and other lipoproteins such as low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicron (CM) may be quantified selectively, simply and accurately. The method for quantifying cholesterol in high density lipoprotein comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein in a test sample, and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of the first step and enzymatically quantifying cholesterol in high density lipoprotein.
    • 本发明公开了一种定量HDL中的胆固醇的方法,其不需要复杂的分离和分离操作,并且含有HDL和其它脂蛋白(例如低密度脂蛋白(LDL),极低密度脂蛋白(VLDL))的测试样品中的HDL胆固醇, 和乳糜微粒(CM)可以选择性,简单和精确地量化。 用于定量高密度脂蛋白中的胆固醇的方法包括在测试样品中除去高密度脂蛋白以外的脂蛋白中的胆固醇的第一步骤,以及将特异性作用于高密度脂蛋白的表面活性剂加入到第一步骤的产物的第二步骤 并在高密度脂蛋白中酶法定量胆固醇。