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    • 4. 发明授权
    • Apparatus and method of portable automated biomonitoring of water quality
    • 水质便携式自动生物监测仪器及方法
    • US06988394B2
    • 2006-01-24
    • US10774639
    • 2004-02-03
    • Tommy R. SheddMark W. WidderMark W. BrownMurray S. Swanson
    • Tommy R. SheddMark W. WidderMark W. BrownMurray S. Swanson
    • G01N33/18A01K63/00
    • G01N33/186
    • A portable automated biomonitoring system for monitoring water quality includes an exposure chamber for housing an aquatic organism and a water inlet for directing water to the exposure chamber. The aquatic organism has ventilatory behavior and body movement sensitive to water quality. Electrodes sense electrical signals produced by the organism during its ventilatory behavior and body movement, and a controller responsive to signals from the electrodes determines a plurality of ventilatory parameters based on the signals. The ventilatory parameters are compared with corresponding thresholds to determine when the water to which the organism is exposed has caused physiological stress to the organism. The water being evaluated may be recirculated through the system for further analysis.
    • 用于监测水质的便携式自动生物监测系统包括用于容纳水生生物的暴露室和用于将水引导到暴露室的进水口。 水生生物具有对水质敏感的通气行为和身体运动。 电极在其通气行为和身体移动期间感测由生物体产生的电信号,并且响应于来自电极的信号的控制器基于该信号确定多个通气参数。 将通气参数与相应的阈值进行比较,以确定生物体暴露的水何时对生物体产生生理压力。 被评估的水可以通过系统再循环以进一步分析。
    • 6. 发明授权
    • Method for rapid toxicity testing of a liquid sample
    • 液体样品快速毒性试验方法
    • US5416005A
    • 1995-05-16
    • US153415
    • 1993-11-15
    • James T. Blankemeyer
    • James T. Blankemeyer
    • G01N33/18C12Q1/02C12Q1/18
    • G01N33/186Y10S435/808
    • A toxicity testing assay wherein a test sample is prepared with electrochromic dye such as Di-4-ANEPPS and a living organism of the cladoceran order, Daphnia Spp. or other test organisms, and the sample is irradiated with alternately blue and yellow light to excite fluorescence. The successive groups of fluorescent emissions are then viewed at 90.degree. by a photomultiplier which develops equivalent alternate count outputs, and the count output is amplified and processed to develop data indicating (1) membrane potential of cells of the living organisms and (2) the total dye fluorescence, which combined effects provides indication of deleterious effects to the organisms.
    • 毒性测试测定,其中测试样品由电致变色染料如Di-4-ANEPPS和克隆克隆的活生物Daphnia Spp制备。 或其他测试生物,并且用交替的蓝色和黄色光照射样品以激发荧光。 然后通过光电倍增管在90°观察连续的荧光发射组,其产生等效的交替计数输出,并且计数输出被放大和处理以产生表明(1)活体细胞的膜电位的数据,和(2) 总染料荧光,其组合效应提供对生物体的有害作用的指示。
    • 7. 发明授权
    • Flow control and diluter system for bioassay testing
    • 用于生物测定的流量控制和稀释系统
    • US5123438A
    • 1992-06-23
    • US797505
    • 1991-11-20
    • Robert S. Henderson
    • Robert S. Henderson
    • G01N1/28G01N1/38G01N33/18
    • G01N1/38G01N33/186G01N2001/2893Y10T137/4673Y10T137/86445Y10T436/25625
    • An apparatus is provided for producing specific concentrations of waterbo pollutants diluted in unfiltered seawater and for periodically delivering the seawater/pollutant dilutions to a plurality of experimental test aquaria at selected flow rates. A seawater feed tank containing the unfiltered seawater drains, under the force of gravity, into a seawater feed trough. The seawater feed trough is constructed to allow the unfiltered seawater to escape therefrom at a plurality of seawater escape locations under the force of gravity. A plurality of seawater cells are aligned beneath the seawater feed trough to receive the unfiltered seawater escaping therefrom. Means are further provided to limit the amount of unfiltered seawater in each of the seawater cells to a preselected amount. In a similar fashion, a pollutant feed tank containing the waterborne pollutants drains under the force of gravity into a pollutant feed trough. The pollutant feed trough is constructed to allow the waterborne pollutants to escape therefrom at a plurality of pollutant escape locations under the force of gravity. A plurality of pollutant cells are aligned beneath the pollutant feed trough to receive the waterborne pollutants escaping therefrom. Means are further provided to limit the amount of waterborne pollutants in each of the pollutant cells to a preselected amount. The seawater and pollutant cells are periodically and simultaneously drained under the force of gravity into a plurality of mixing chambers. Each mixing chamber receives the unfiltered seawater drained from one of the seawater cells and pollutants drained from one of the pollutant cells. The resulting seawater/pollutant dilution formed in each of the mixing chambers is then delivered under the force of gravity to one of the experimental test aquaria.
    • 提供了一种用于产生在未经过滤的海水中稀释的特定浓度的水性污染物的设备,并且以选定的流速将海水/污染物稀释液周期性地递送到多个实验测试水族箱。 在重力作用下将含有未过滤海水的海水进料罐排入海水进料槽。 海水进料槽被构造成允许未过滤的海水在重力作用下在多个海水逃逸位置从其中逸出。 多个海水细胞在海水进料槽下方对准以接收从其中逸出的未过滤的海水。 进一步提供了将每个海水细胞中的未过滤海水的量限制为预选量的方法。 以类似的方式,含有水污染物质的污染物进料罐在重力作用下排入污染物进料槽。 污染物进料槽被构造成允许水分污染物在重力作用下在多个污染物逸出处逃逸。 多个污染细胞在污染物进料槽下方排列,以接收从中排出的水性污染物。 进一步提供了将每个污染细胞中的水性污染物的量限制在预选量的手段。 周期性地将海水和污染细胞在重力作用下排放到多个混合室中。 每个混合室都接收从一个海水细胞排出的未过滤的海水和从一个污染细胞排出的污染物。 然后在每个混合室中形成的所形成的海水/污染物稀释液在重力作用下输送到实验试验水族箱之一。