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1. 发明申请

US20170166942A1 OVEREXPRESSION OF N-GLYCOSYLATION PATHWAY REGULATORS TO MODULATE GLYCOSYLATION OF RECOMBINANT PROTEINS 审中-公开
公开(公告)号：US20170166942A1
公开(公告)日：2017-06-15
申请号：US15115615
申请日：2014-12-11
申请人： AMGEN INC.
发明人： Shivani GUPTA , Sohye KANG
IPC分类号： C12P21/00 , C07K14/705 , C07K16/00 , C12N9/10
CPC分类号： C12P21/005 , C07K14/705 , C07K16/00 , C07K2317/41 , C12N9/1051 , C12Y204/01101 , C12Y204/01143
摘要： Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

2. 发明授权

US09175278B2 Method of producing recombinant proteins with mannose-terminated N-glycans 有权
标题翻译：用甘露糖封端的N-聚糖制备重组蛋白的方法
公开(公告)号：US09175278B2
公开(公告)日：2015-11-03
申请号：US13636952
申请日：2011-03-24
申请人： Zhiwei Song
发明人： Zhiwei Song
IPC分类号： C12P21/06 , C12N9/42 , C12P21/00 , C12N9/24
CPC分类号： C12N9/2445 , C12N9/2402 , C12P21/005 , C12Y204/01101 , C12Y302/01045
摘要： We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-1 into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.
摘要翻译：我们描述了从宿主细胞中表达包含甘露糖封端的N-聚糖的重组蛋白的方法，所述方法包括：（a）将编码重组蛋白的核酸引入中国仓鼠卵巢（CHO）细胞中，所述中国仓鼠卵巢（CHO）细胞包含 GnT 1基因（GenBank登录号AF343963）导致GnT1功能丧失; 和（c）从宿主细胞中表达重组蛋白，其中表达的重组蛋白包含甘露糖封端的聚糖结构，并且其中该方法不包括将功能性GnT-1引入宿主细胞的步骤。该方法可用于生产具有甘露糖封端的聚糖结构的重组葡糖脑苷脂酶，适用于治疗或预防戈谢氏病。

3. 发明申请

US20150152402A1 METHOD OF PRODUCING RECOMBINANT PROTEINS WITH MANNOSE-TERMINATED N-GLYCANS 有权
标题翻译：用万能终止的N-甘露糖生产重组蛋白的方法
公开(公告)号：US20150152402A1
公开(公告)日：2015-06-04
申请号：US14620856
申请日：2015-02-12
申请人： Agency for Science, Technology and Research
发明人： Zhiwei Song
IPC分类号： C12N9/42 , C12P21/00
CPC分类号： C12N9/2445 , C12N9/2402 , C12P21/005 , C12Y204/01101 , C12Y302/01045
摘要： We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-I into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.
摘要翻译：我们描述了从宿主细胞中表达包含甘露糖封端的N-聚糖的重组蛋白的方法，所述方法包括：（a）将编码重组蛋白的核酸引入中国仓鼠卵巢（CHO）细胞中，所述中国仓鼠卵巢（CHO）细胞包含 GnT 1基因（GenBank登录号AF343963）导致GnT1功能丧失; 和（c）从宿主细胞中表达重组蛋白，其中表达的重组蛋白包含甘露糖封端的聚糖结构，并且其中该方法不包括将功能性GnT-I引入宿主细胞的步骤。该方法可用于生产具有甘露糖封端的聚糖结构的重组葡糖脑苷脂酶，适用于治疗或预防戈谢氏病。

4. 发明申请

US20140349341A1 PRODUCTION OF RECOMBINANT PROTEINS WITH SIMPLE GLYCOFORMS 有权
标题翻译：用简单的糖浆生产重组蛋白
公开(公告)号：US20140349341A1
公开(公告)日：2014-11-27
申请号：US14371137
申请日：2013-01-10
申请人： SIGMA-ALDRICH CO. LLC
发明人： Nan Lin , Natalie Sealover , Henry George , Kevin Kayser
IPC分类号： C07K16/00
CPC分类号： C07K16/10 , C07K16/00 , C07K2317/14 , C07K2317/41 , C12N9/1051 , C12P21/005 , C12P21/02 , C12Y204/01101 , Y02P20/52
摘要： The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.
摘要翻译：本发明提供缺乏甘露糖基（α-1,3 - ） - 糖蛋白β-1,2-N-乙酰葡糖胺基转移酶I（Mgat1）的细胞系。还提供了用于产生Mga1缺陷型细胞系的方法和使用Mgat1缺陷型细胞系生产具有简单糖型重组蛋白质的方法。

5. 发明申请

US20130164798A1 PROCESSES FOR PRODUCING HYDROCARBON PRODUCTS 有权
公开(公告)号：US20130164798A1
公开(公告)日：2013-06-27
申请号：US13725404
申请日：2012-12-21
申请人： Thomas Vanhercke , James Robertson Petrie , Anna El Tahchy , Surinder Pal Singh , Qing Liu
发明人： Thomas Vanhercke , James Robertson Petrie , Anna El Tahchy , Surinder Pal Singh , Qing Liu
IPC分类号： C10L1/04 , C12P7/64 , C12P7/16 , C07C1/207 , C12P7/04 , C12P5/02 , C07C67/00 , C07C29/147 , C10L5/44 , C12P7/06
CPC分类号： C10B53/02 , C01B3/22 , C01B32/40 , C01B2203/1211 , C07C67/48 , C07K14/415 , C10G2/00 , C10G2/30 , C10J3/82 , C10J2300/0906 , C10J2300/0916 , C10J2300/1659 , C10J2300/1846 , C10L1/02 , C10L1/026 , C10L5/44 , C10L2200/0469 , C10L2200/0476 , C10L2200/0492 , C10L2270/026 , C10L2290/02 , C10L2290/04 , C10L2290/06 , C10L2290/26 , C10L2290/28 , C10L2290/30 , C10L2290/42 , C10L2290/544 , C11B1/00 , C11B1/10 , C11B3/001 , C11B3/006 , C11B3/10 , C11B3/14 , C11B7/0075 , C11C3/003 , C12N9/1051 , C12N15/8218 , C12N15/8247 , C12P7/6463 , C12Y204/01101 , Y02E20/16 , Y02E20/18 , Y02E50/10 , Y02E50/13 , Y02E50/14 , Y02E50/17 , Y02E50/30 , Y02E50/32 , Y02E50/343
摘要： The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.

6. 发明公开

US20240287568A1 Engineered and Fully-Functional Customized Glycoproteins 审中-公开
公开(公告)号：US20240287568A1
公开(公告)日：2024-08-29
申请号：US18404577
申请日：2024-01-04
申请人： LimmaTech Biologics AG
发明人： Amirreza Faridmoayer , Manuela Mally , Rainer Follador
IPC分类号： C12P21/00 , C12N1/10 , C12N9/10 , C12R1/90
CPC分类号： C12P21/005 , C12N1/105 , C12N9/1029 , C12N9/1051 , C12N9/1081 , C07K2319/04 , C07K2319/05 , C12R2001/90 , C12Y203/01003 , C12Y204/01022 , C12Y204/01101 , C12Y204/01143 , C12Y204/99006
摘要： Described herein are compositions and methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof.

7. 发明申请

US20170002337A1 Fusion Enzymes 审中-公开
标题翻译：融合酶
公开(公告)号：US20170002337A1
公开(公告)日：2017-01-05
申请号：US15205577
申请日：2016-07-08
申请人： Glykos Finland Oy
发明人： Jari Natunen , Anne Kanerva , Jukka Hiltunen , Markku Saloheimo , Heli Viskari , Anne Huuskonen
IPC分类号： C12N9/10 , C12N15/80
CPC分类号： C12N9/1051 , C07K2319/03 , C12N15/80 , C12P19/18 , C12P21/005 , C12Y204/01101 , C12Y204/01143 , Y02P20/52
摘要： The present disclosure relates to recombinant proteins having N-acetylglucosaminyltransferase activity. The present disclosure further relates to methods for producing complex N-glycans including the steps of providing host cells containing such recombinant proteins and culturing the host cells such that the recombinant proteins are expressed.
摘要翻译：本公开涉及具有N-乙酰葡糖胺转移酶活性的重组蛋白。本公开进一步涉及生产复合N-聚糖的方法，包括提供含有这种重组蛋白的宿主细胞并培养宿主细胞以使表达重组蛋白的步骤。

8. 发明申请

US20160369240A1 VIRAL RESISTANT CELLS AND USES THEREOF 审中-公开
标题翻译：抗病毒细胞及其用途
公开(公告)号：US20160369240A1
公开(公告)日：2016-12-22
申请号：US15122311
申请日：2015-03-03
申请人： SIGMA-ALDRICH CO. LLC
发明人： Nan LIN , Joaquina MASCARENHAS , Audrey CHANG , David ONIONS , Henry GEORGE , Kevin KAYSER
IPC分类号： C12N5/071 , C12N9/10
CPC分类号： C12N5/0682 , C12N5/00 , C12N9/1051 , C12N2501/724 , C12N2510/02 , C12N2720/12011 , C12N2750/14311 , C12Y204/01101 , C12Y204/01144 , C12Y204/01155
摘要： The present invention provides mammalian cell lines that have been genetically engineered causing such cell lines to be resistant to viral entry and/or propagation, and provides methods of using said cell lines to reduce or prevent viral contamination of biologic production systems.
摘要翻译：本发明提供已经进行遗传工程改造的哺乳动物细胞系，使得这种细胞系能够抵抗病毒进入和/或繁殖，并提供使用所述细胞系减少或防止生物制备系统的病毒污染的方法。

9. 发明授权

US09399764B2 Fusion enzymes 有权
标题翻译：融合酶
公开(公告)号：US09399764B2
公开(公告)日：2016-07-26
申请号：US13989084
申请日：2011-11-24
申请人： Jari Natunen , Anne Kanerva , Jukka Hiltunen , Markku Saloheimo , Heli Viskari , Anne Huuskonen
发明人： Jari Natunen , Anne Kanerva , Jukka Hiltunen , Markku Saloheimo , Heli Viskari , Anne Huuskonen
IPC分类号： C12N9/10 , C12P21/00 , C12P19/18 , C07K19/00 , C12N15/80
CPC分类号： C12N9/1051 , C07K2319/03 , C12N15/80 , C12P19/18 , C12P21/005 , C12Y204/01101 , C12Y204/01143 , Y02P20/52
摘要： The present disclosure relates to recombinant proteins having N-acetylglucosaminyltransferase activity. The present disclosure further relates to methods for producing complex N-glycans including the steps of providing host cells containing such recombinant proteins and culturing the host cells such that the recombinant proteins are expressed.
摘要翻译：本公开涉及具有N-乙酰葡糖胺转移酶活性的重组蛋白。本公开进一步涉及生产复合N-聚糖的方法，包括提供含有这种重组蛋白的宿主细胞并培养宿主细胞以使表达重组蛋白的步骤。

10. 发明授权

US08735111B2 Processes for producing hydrocarbon products 有权
标题翻译：生产烃类产品的工艺
公开(公告)号：US08735111B2
公开(公告)日：2014-05-27
申请号：US13725404
申请日：2012-12-21
申请人： Thomas Vanhercke , James Robertson Petrie , Anna El Tahchy , Surinder Pal Singh , Qing Liu
发明人： Thomas Vanhercke , James Robertson Petrie , Anna El Tahchy , Surinder Pal Singh , Qing Liu
IPC分类号： A01H5/00 , C12N15/52
CPC分类号： C10B53/02 , C01B3/22 , C01B32/40 , C01B2203/1211 , C07C67/48 , C07K14/415 , C10G2/00 , C10G2/30 , C10J3/82 , C10J2300/0906 , C10J2300/0916 , C10J2300/1659 , C10J2300/1846 , C10L1/02 , C10L1/026 , C10L5/44 , C10L2200/0469 , C10L2200/0476 , C10L2200/0492 , C10L2270/026 , C10L2290/02 , C10L2290/04 , C10L2290/06 , C10L2290/26 , C10L2290/28 , C10L2290/30 , C10L2290/42 , C10L2290/544 , C11B1/00 , C11B1/10 , C11B3/001 , C11B3/006 , C11B3/10 , C11B3/14 , C11B7/0075 , C11C3/003 , C12N9/1051 , C12N15/8218 , C12N15/8247 , C12P7/6463 , C12Y204/01101 , Y02E20/16 , Y02E20/18 , Y02E50/10 , Y02E50/13 , Y02E50/14 , Y02E50/17 , Y02E50/30 , Y02E50/32 , Y02E50/343
摘要： The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.
摘要翻译：本发明涉及从营养植物部分中的非极性脂质生产工业产品如烃产物的方法。优选的工业产品包括可与石油基燃料混合的烷基酯。

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