专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

US20230203557A1 IN VITRO GLYCOSYLATION OF PROTEINS AND ENZYMES 审中-公开
公开(公告)号：US20230203557A1
公开(公告)日：2023-06-29
申请号：US18170045
申请日：2023-02-16
申请人： Design-Zyme LLC
发明人： Peter Albert Petillo , Dwight O'Dell Deay, III
IPC分类号： C12Q1/00 , C12P21/00 , C07K1/107 , C12N9/04 , C12N9/02 , C07K1/00
CPC分类号： C12Q1/005 , C12P21/005 , C07K1/1077 , C12N9/0006 , C12N9/0004 , C07K1/006
摘要： The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.

2. 发明申请

US20170342104A1 COUPLING METHOD FOR PEPTIDE SYNTHESIS AT ELEVATED TEMPERATURES 有权
公开(公告)号：US20170342104A1
公开(公告)日：2017-11-30
申请号：US15647909
申请日：2017-07-12
申请人： CEM CORPORATION
发明人： JONATHAN M. COLLINS , SANDEEP KUMAR SINGH
IPC分类号： C07K1/00 , C07K1/04 , C07K1/08 , C07K14/575
CPC分类号： C07K1/006 , C07K1/04 , C07K1/045 , C07K1/08 , C07K1/084 , C07K1/10 , C07K14/57581
摘要： An improved method for coupling amino acids into peptides or peptidomimetics is disclosed that includes the steps of combining an amino acid, a carbodiimide, an activator additive, and a base at less than 1 equivalent compared to the amino acid to be activated; and carrying out the activation and coupling at a temperature greater than 30° C.

3. 发明授权

US09670257B2 Methods for producing peptides using engineered inteins 有权
公开(公告)号：US09670257B2
公开(公告)日：2017-06-06
申请号：US14893349
申请日：2014-05-28
申请人： Novo Nordisk A/S
发明人： Allan Christian Shaw , Jens Christian Norrild , Louise Albertsen
IPC分类号： C07K14/47 , C07K14/575 , C07K1/00 , C12N15/62 , C12N9/90 , C07K7/00 , C12N15/09
CPC分类号： C07K14/47 , C07K1/006 , C07K7/00 , C07K14/575 , C07K2319/00 , C07K2319/92 , C12N9/90 , C12N15/09 , C12N15/625
摘要： The present invention provides a method for producing peptides by recombinant means. The peptides are expressed as part of a fusion protein comprising the target peptide and an engineered intein. The invention also provides the engineered inteins, fusion proteins comprising these, and DNA constructs coding for these fusion proteins. Upon thiol-induced cleavage of the fusion protein the carboxy-terminal a-thioester of the target peptide is obtained. The carboxy-terminal α-thioester can in principle react with any nucleophile and the strategy therefore allows a wider range of carboxy-terminal modifications such as chemical ligation, bioconjugation, or amidation. The engineered inteins of the present invention are minimized in size and has a cysteine mutation in the position corresponding by alignment to position 3 of Mxe GyrA intein (SEQ ID NO:1) leading to increased expression levels of the fusion protein and higher yields of the isolated target peptide, thus making the method of the invention suitable for production scale.

4. 发明授权

US09416156B2 Method of making biologically active alpha-beta peptides 有权
标题翻译：制备生物活性α-β肽的方法
公开(公告)号：US09416156B2
公开(公告)日：2016-08-16
申请号：US14150125
申请日：2014-01-08
申请人： Wisconsin Alumni Research Foundation
发明人： William S. Horne , Samuel H. Gellman , Lisa M. Johnson
IPC分类号： A01N37/18 , A61K38/00 , A61P31/18 , C07K5/00 , C07K7/00 , C07K16/00 , C07K17/00 , C07K1/00 , C07K7/02 , C07K14/00 , C07K14/005 , C07K14/47 , C07K1/107
CPC分类号： C07K1/006 , C07K1/107 , C07K7/02 , C07K14/00 , C07K14/001 , C07K14/005 , C07K14/4702 , C12N2740/16122
摘要： Described is a method of fabricating biologically active, unnatural polypeptides. The method includes the steps of selecting a biologically active polypeptide or biologically active fragment thereof having an amino acid sequence comprising α-amino acid residues, and fabricating a synthetic polypeptide that has an amino acid sequence that corresponds to the sequence of the biologically active polypeptide, but wherein about 14% to about 50% of the α-amino acid residues found in the biologically active polypeptide or fragment of step (a) are replaced with β-amino acid residues, and the α-amino acid residues are distributed in a repeating pattern.
摘要翻译：描述了制造生物活性的非天然多肽的方法。该方法包括以下步骤：选择具有包含α-氨基酸残基的氨基酸序列的生物活性多肽或其生物活性片段，并制备具有与生物活性多肽序列相对应的氨基酸序列的合成多肽，但其中在生物活性多肽或步骤（a）的片段中发现的α-氨基酸残基中约14％至约50％被β-氨基酸残基替代，并且α-氨基酸残基以重复的模式。

5. 发明授权

US09260479B2 Cyclic amino acid molecules and methods of preparing the same 有权
标题翻译：环状氨基酸分子及其制备方法
公开(公告)号：US09260479B2
公开(公告)日：2016-02-16
申请号：US13257159
申请日：2010-03-16
申请人： Andrei Yudin , Ryan Hili
发明人： Andrei Yudin , Ryan Hili
IPC分类号： C07K5/12 , C07K7/00 , C07K1/02 , C07D487/04 , C07D487/14 , C07K7/64
CPC分类号： C07K7/64 , C07D487/04 , C07D487/14 , C07F7/1804 , C07K1/003 , C07K1/006 , C07K5/12 , C07K5/123 , C07K5/126
摘要： Macrocyclization of amino acids or linear peptides upon reaction with amphoteric amino aldehydes and isocyanides is provided.
摘要翻译：提供了与两性氨基醛和异氰酸酯反应时氨基酸或线性肽的大环化。

6. 发明申请

US20150329590A1 Cysteine Arylation Directed by a Genetically Encodable Pi-Clamp 有权
标题翻译：由可遗传编码的Pi-Clamp引导的半胱氨酸酰化
公开(公告)号：US20150329590A1
公开(公告)日：2015-11-19
申请号：US14278060
申请日：2014-05-15
申请人： Bradley L. Pentelute , Chi Zhang
发明人： Bradley L. Pentelute , Chi Zhang
IPC分类号： C07K5/087
CPC分类号： C07K1/13 , A61K38/00 , C07K1/006 , C07K5/0812 , C07K5/0817
摘要： Disclosed are methods of single-site-specific cysteine modification on peptide/protein molecules under physiologically relevant conditions. This process features several significant advantages over existing methods of peptide modification, such as specificity towards thiols over other nucleophiles (e.g., amines, hydroxyls), excellent functional group tolerance, and mild reaction conditions. Especially important is the specificity observed for thiols appearing in an X-Cys-Pro-X sequence over other thiols or disulfides, where X is Phe, Trp, or Tyr; under the inventive conditions, other cysteines or reactive functional groups on the same peptide/protein chain are not functionalized.
摘要翻译：公开了在生理相关条件下对肽/蛋白质分子进行单位点特异性半胱氨酸修饰的方法。该方法具有优于现有的肽修饰方法的几个显着优点，例如对硫醇比其它亲核试剂（例如胺，羟基）的特异性，优异的官能团耐受性和温和的反应条件。特别重要的是在X-Cys-Pro-X序列中出现的硫醇与其它硫醇或二硫化物的特异性，其中X是Phe，Trp或Tyr; 在本发明条件下，相同肽/蛋白质链上的其它半胱氨酸或反应性官能团未被官能化。

7. 发明授权

US09073978B2 Method for producing glycopeptide having sialyl sugar chain, sialyl sugar chain-added amino acid derivative to be used in same, and glycopeptide 有权
标题翻译：制备具有唾液酸糖链的糖肽的方法，用于其中的唾液酸糖链加成氨基酸衍生物和糖肽
公开(公告)号：US09073978B2
公开(公告)日：2015-07-07
申请号：US14004122
申请日：2012-03-05
申请人： Yasuhiro Kajihara , Masumi Murakami , Kazuyuki Ishii
发明人： Yasuhiro Kajihara , Masumi Murakami , Kazuyuki Ishii
IPC分类号： C07H1/00 , C07K1/06 , C07K14/00 , C07K1/00
CPC分类号： C07K14/001 , C07H1/00 , C07K1/006 , C07K1/068
摘要： [Technical Problem]To provide a method for manufacturing that enables to obtain a targeted glycopeptide harboring a sialyl sugar chain in high yield without decomposing sialic acid at a non-reducing terminal of sugar chain when the glycopeptide is synthesized by a Boc solid phase synthesis method.[Solution to Problem]The present invention is characterized in that the Boc-sialylglycosylated amino acid derivative used in Boc solid phase synthesis method is one where the carboxyl group of the sialic acid at the sugar chain non-reducing terminal is protected with a phenacyl group.
摘要翻译：技术问题提供一种制造方法，当通过Boc固相合成方法合成糖肽时，能够以高产率获得含有唾液酸糖链的目标糖肽，而不会在糖链的非还原末端分解唾液酸。 [问题的解决方案]本发明的特征在于Boc固相合成法中使用的Boc-唾液酸糖基化氨基酸衍生物，其中在糖链非还原末端的唾液酸的羧基被苯甲酰基保护。

8. 发明申请

US20150164988A1 PAN-ANTIVIRAL PEPTIDES AND USES THEREOF 有权
标题翻译：泛抗病毒肽及其用途
公开(公告)号：US20150164988A1
公开(公告)日：2015-06-18
申请号：US14579650
申请日：2014-12-22
申请人： Nuovo Biologics, LLC
发明人： Kent D. MILLER , Billy S. AUSTIN
IPC分类号： A61K38/17 , C12N7/00
CPC分类号： A61K38/1767 , A61K38/00 , C07K1/006 , C07K14/46 , C12N7/00 , C12N2760/18063
摘要： Methods for preparing viral neuraminidase inhibitors including antiviral peptides by specifically chemically modifying disulfide bonds in precursor molecules. A method of inhibiting viral neuraminidases by administrating a viral neuraminidase inhibitor comprising an antiviral peptide prepared by the above methods and inhibiting the viral neuraminidase. Therapeutics for inhibiting viral neuraminidases, including effective amounts of viral neuraminidase inhibitors including antiviral peptides derived from selectively chemically modified disulfide bonds in precursor molecules, and present in a pharmaceutically acceptable carriers.
摘要翻译：通过在前体分子中特异化学改性二硫键来制备包括抗病毒肽的病毒神经氨酸酶抑制剂的方法。通过施用包含通过上述方法制备的抗病毒肽并抑制病毒神经氨酸酶的病毒神经氨酸酶抑制剂来抑制病毒神经氨酸酶的方法。用于抑制病毒神经氨酸酶的治疗剂，包括有效量的病毒神经氨酸酶抑制剂，包括衍生自前体分子中选择性化学修饰的二硫键的抗病毒肽，并且存在于药学上可接受的载体中。

9. 发明申请

US20150072398A1 Aldehyde Tags, Uses Thereof in Site-Specific Protein Modification 有权
标题翻译：醛标签，其在特定于位点的蛋白质修饰中的应用
公开(公告)号：US20150072398A1
公开(公告)日：2015-03-12
申请号：US14464528
申请日：2014-08-20
申请人： The Regents of the University of California
发明人： Isaac S. Carrico , Brian L. Carlson , Peng Wu , Carolyn Bertozzi
IPC分类号： C12N9/10 , C07K14/35 , C07K14/565 , C07K14/00 , C07K16/00
CPC分类号： C07K16/00 , A61K38/1825 , A61K38/215 , A61K38/37 , A61K38/45 , A61K39/00 , C07K1/006 , C07K1/1072 , C07K1/13 , C07K14/00 , C07K14/35 , C07K14/565 , C07K16/46 , C07K2317/24 , C07K2317/40 , C07K2317/52 , C07K2317/622 , C12N9/13 , C12N9/96 , C12P21/00 , C12P21/005 , C12Y208/02
摘要： The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.
摘要翻译：本发明的特征在于通过掺入醛标记蛋白的位点特异性修饰的组合物和方法。通过甲酰甘氨酸生成酶（FGE）的作用，在醛标记的硫酸酯酶基序上进行酶促修饰产生甲酰甘氨酸（FGly）残基。 FGly残基的醛部分可以用作化学处理，用于将目标部分的位点特异性连接到多肽。

10. 发明申请

US20140142044A1 PEPTIDE MIMETIC LIGANDS OF POLO-LIKE KINASE 1 POLO BOX DOMAIN AND METHODS OF USE 审中-公开
标题翻译： POLO-like激素的肽类化合物1 POLO BOX域及其使用方法
公开(公告)号：US20140142044A1
公开(公告)日：2014-05-22
申请号：US14111540
申请日：2012-04-12
申请人： Terrence R. Burke, JR. , Wenjian Qian , Fa Liu , Kyung S. Lee , Jung-Eun Park
发明人： Terrence R. Burke, JR. , Wenjian Qian , Fa Liu , Kyung S. Lee , Jung-Eun Park
IPC分类号： C07K7/06
CPC分类号： C07K7/06 , A61K38/00 , A61K47/60 , C07K1/006 , C07K5/0827 , C12N9/12 , C12Y207/11021
摘要： Novel compounds are provided that bind to polo-like kinases through the polo-box domain. In certain embodiments, the novel compounds are PEGylated peptides. The PEGylated peptides in accordance with the invention demonstrate high PBD-binding affinity. In certain embodiments, the PEGylated peptides have also achieved activities in whole cell systems. The invention also provides compounds that bind polo-like kinases through the polo-box domain and possess reduced anionic charge. Further provided are methods of design and/or synthesis of the PEGylated peptides and methods of use thereof. The invention provides methods of use of the compounds and methods of synthesis of the compounds. The compounds of the invention have potential therapeutic activity in view of their binding and inhibitory activities towards Plk1. They are based on the amino acid sequence PLHSpT (phosphorylated Thr). The PEG moiety, when present, is covalently attached at the N-terminus. The invention also encompasses derivatives having substituents on the Pro and/or the His side chains, substituents on the phosphate group, the replacement of His with Gln, the replacement of Pro with a N-substituted Gly, the replacement of Ser with Ala, as well as HS-pT fragments with N-terminal alkoxycarbonyl group and PLHS fragments with a C-terminal glyco-imino-alkylxyalkylamide group.
摘要翻译：提供了通过polo-box结构域结合polo样激酶的新型化合物。在某些实施方案中，新化合物是PEG化肽。根据本发明的聚乙二醇化肽表现出高PBD结合亲和力。在某些实施方案中，聚乙二醇化肽还在整个细胞系统中获得活性。本发明还提供通过马球结构域结合polo样激酶并具有减少的阴离子电荷的化合物。还提供了聚乙二醇化肽的设计和/或合成方法及其使用方法。本发明提供了化合物的使用方法和化合物的合成方法。考虑到它们对Plk1的结合和抑制活性，本发明的化合物具有潜在的治疗活性。它们基于氨基酸序列PLHSpT（磷酸化的Thr）。存在时，PEG部分在N末端共价连接。本发明还包括在Pro和/或His侧链上具有取代基的衍生物，磷酸基团上的取代基，用Gln取代His，用N-取代的Gly取代Pro，用Ala取代Ser，作为以及具有N-末端烷氧基羰基的HS-pT片段和具有C-末端糖 - 亚氨基 - 烷基烷基酰胺基团的PLHS片段。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式