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1. 发明申请

US20140171635A1 NOVEL HYDRAZONE-BASED AND OXIME-BASED FLUORESCENT AND CHROMOPHORIC/PRO-FLUORESCENT AND PRO-CHROMOPHORIC REAGENTS AND LINKERS 审中-公开
标题翻译：新型基于液相色谱和基于氧化物的荧光和染色体/荧光染料和预染色试剂和连接物
公开(公告)号：US20140171635A1
公开(公告)日：2014-06-19
申请号：US13967086
申请日：2013-08-14
申请人： Solulink Biosciences, Inc.
发明人： David A. Schwartz , Leopold Mendoza
IPC分类号： G01N33/58
CPC分类号： G01N33/582 , C07D303/02
摘要： Novel Hydrazone-based Fluorescent and Pro-Fluorescent Reagents and Linkers, including conjugationally extended hydrazine compositions, fluorescent hydrazone compositions, methods of the formation of hydrazones from the reaction of conjugationally extended hydrazines with conjugationally extended carbonyls, and methods of their use in assays systems are described. Use of these conjugationally compositions for direct colorimetric and fluorometric assays wherein a chromophore or the fluorophore is incorporated into the linker that is positioned between a reactive linking moiety and a biotin molecule.
摘要翻译：新型腙类荧光和荧光试剂和连接剂，包括共轭延伸的肼组合物，荧光腙组合物，从共轭延伸的肼与共轭延伸的羰基反应形成腙的方法及其在测定系统中的应用方法描述。使用这些共轭组合物用于直接比色和荧光测定，其中发色团或荧光团掺入位于反应性连接部分和生物素分子之间的接头中。

2. 发明授权

US08541555B2 Hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers 有权
标题翻译：腙基和肟基荧光和发色/前荧光和原色发色试剂和接头
公开(公告)号：US08541555B2
公开(公告)日：2013-09-24
申请号：US11787932
申请日：2007-04-18
申请人： David A. Schwartz , Leopoldo Mendoza
发明人： David A. Schwartz , Leopoldo Mendoza
IPC分类号： C07K16/00 , C07H21/04 , C07D235/02
CPC分类号： G01N33/582 , C07D303/02
摘要： Conjugationally extended hydrazine compositions of the formula (RR2)N(H)n(NH2)n, fluorescent hydrazone compositions of the formula (RR2)NN═C(R1R2), methods of the formation of hydrazones from the reaction of conjugationally extended hydrazines with conjugationally extended carbonyls and methods of their use in assays systems are described. Use of these conjugationally extended hydrazine and oxime compositions for direct calorimetric and fluorometric assays wherein a chromophore or the fluorophore is incorporated into the linker that is positioned between a reactive linking moiety and a biotin molecule. More specifically the linker comprises one molecule of a high affinity binding pair such as for example biotin of the biotin/avidin high affinity binding pair, connected to a spacer molecule such as for example a length of polyethyleneglycol followed by a pro-chromophoric, chromophoric, pro-fluorescent or fluorescent moiety connected to an amino-, thiol- or carbohydrate-reactive moiety such as for example succinimidyl, maleimido or aminoxy group respectively, that may covalently link to a biomolecule.
摘要翻译：式（RR2）N（H）n（NH2）n的共轭延伸的肼组合物，式（RR2）NN = C（R1R2）的荧光腙组合物，从共轭延伸的肼与描述了缀合扩展的羰基及其在测定系统中的用途。使用这些共轭延伸的肼和肟组合物用于直接量热和荧光测定，其中发色团或荧光团被引入位于反应性连接部分和生物素分子之间的连接体中。更具体地，接头包含一个高亲和力结合对分子，例如生物素/抗生物素蛋白高亲和力结合对的生物素，连接到间隔分子，例如长度的聚乙二醇，随后是发色团，发色团，分别连接到可以共价连接到生物分子的氨基，硫醇或碳水化合物反应性部分的前荧光或荧光部分，例如琥珀酰亚胺基，马来酰亚胺基或氨氧基。

3. 发明授权

US07462689B2 Hydrazine-based and carbonyl-based bifunctional crosslinking reagents 有权
标题翻译：基于肼和羰基的双功能交联试剂
公开(公告)号：US07462689B2
公开(公告)日：2008-12-09
申请号：US10720843
申请日：2003-11-24
申请人： David A. Schwartz
发明人： David A. Schwartz
IPC分类号： C07C281/06 , C07K1/113 , C07K17/00
CPC分类号： C07D207/452 , A61K47/61 , A61K47/62 , A61K47/643 , A61K47/645 , A61K47/6801 , C07D207/46 , C07D213/82 , C07D401/12 , C07F7/1804 , C07K1/1077 , C08G65/329 , C08G65/336 , C08L2203/02 , C12N9/96 , Y10S530/81
摘要： Reagents and methods are provided for crosslinking and immobilizing biomolecules, drugs and synthetic polymers. The reagents possess (i) a thiol or amino reactive group; and (ii) a hydrazino or oxyamino moiety. Conjugates and immobilized biomolecules are also provided.
摘要翻译：提供了用于交联和固定生物分子，药物和合成聚合物的试剂和方法。试剂含有（i）硫醇或氨基反应性基团; 和（ii）肼基或氧基氨基部分。还提供了缀合物和固定的生物分子。

4. 发明授权

US07999098B2 Triphosphate oligonucleotide modification reagents and uses thereof 失效
标题翻译：三磷酸寡核苷酸修饰试剂及其用途
公开(公告)号：US07999098B2
公开(公告)日：2011-08-16
申请号：US11638062
申请日：2006-12-11
申请人： David A. Schwartz , Richard I. Hogrefe
发明人： David A. Schwartz , Richard I. Hogrefe
IPC分类号： C07H21/00
CPC分类号： C07H21/04 , C07H19/00 , C07H19/04 , C07H21/00
摘要： Hydrazino, oxyamino and carbonyl-based monomers and methods for incorporation into oligonucleotides during enzymatic synthesis are provided. Modified oligonucleotides are provided that incorporate the monomers provided herein. Immobilized oligonucleotides and oligonucleotide conjugates that contain covalent hydrazone or oxime linkages are provided. Methods for preparation of surface bound oligonucleotides are provided. Methods for the preparation of oligonucleotide conjugates are also provided.
摘要翻译：提供了肼基，氧基氨基和羰基单体以及在酶合成期间掺入寡核苷酸的方法。提供了引入本文提供的单体的修饰的寡核苷酸。提供含有共价腙或肟键的固定寡核苷酸和寡核苷酸缀合物。提供了制备表面结合寡核苷酸的方法。还提供了制备寡核苷酸缀合物的方法。

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