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    • 6. 发明授权
    • Mixtures of triglycerides of natural polyunsaturated fatty acids with high polyunsaturated fatty acid content, method for producing same and use thereof
    • 具有高多不饱和脂肪酸含量的天然多不饱和脂肪酸的甘油三酯的混合物,其制备方法及其用途
    • US07588791B2
    • 2009-09-15
    • US10493116
    • 2002-10-16
    • Dirk Fabritius
    • Dirk Fabritius
    • A23D9/00
    • C11B7/0075A23D9/00A23L33/115A23V2002/00C11B7/0008A23V2250/1882A23V2250/194
    • A process for increasing the concentration of PUFA triglycerides present in natural PUFA oils, in which a natural PUFA oil having a triglyceride content of more than 85% by weight, based on the total weight of the mixture, and having a total PUFA content of more than 39% by weight of total fatty acids is dissolved in an organic solvent or an organic solvent mixture, the mixture is allowed to stand at a temperature of from −35° C. to −100° C. for a period of more than five minutes, the mixture is separated into a supernatant comprising PUFA-triglyceride mixture and a sediment phase, the separation preferably taking place by filtration or centrifugation, and the solvent or solvent mixture, after the removal of the sediment phase, is separated from the supernatant. PUFA-triglyceride mixtures having a PUFA content of more than 55% by weight of total fatty acids of a main PUFA, or at least two PUFAs selected from stearidonic acid, eicosapentaenoic acid, docosapentaenoic acid, docosahexaenoic acid, gamma-linolenic acid and arachidonic acid, and also use thereof in pharmaceutical, cosmetics and food preparations.
    • 增加存在于天然PUFA油中的PUFA甘油三酯浓度的方法,其中基于混合物的总重量,甘油三酯含量大于85重量%的天然PUFA油,并且具有更多的PUFA含量 将总重量为39重量%的总脂肪酸溶解在有机溶剂或有机溶剂混合物中,将混合物在-35℃至-100℃的温度下静置超过五分钟 分钟,将混合物分离成包含PUFA-甘油三酯混合物和沉淀相的上清液,优选通过过滤或离心进行分离,并且将去除沉淀相后的溶剂或溶剂混合物与上清液分离。 PUFA-甘油三酸酯混合物,其PUFA含量大于主PUFA的总脂肪酸的55重量%,或至少两种选自十八碳四烯酸,二十碳五烯酸,二十二碳五烯酸,二十二碳六烯酸,γ-亚麻酸和花生四烯酸的PUFA ,还可用于制药,化妆品和食品制剂。
    • 9. 发明授权
    • Screening method for identifying PUFA PKS in samples
    • 用于鉴定样品中PUFA PKS的筛选方法
    • US08409798B2
    • 2013-04-02
    • US11547948
    • 2005-04-08
    • Markus LuyMatthias Rüsing
    • Markus LuyMatthias Rüsing
    • C12Q1/68
    • C12Q1/689
    • The invention relates to a method for the rapid and simple identification of PUFA PKS in microorganisms. Said method is characterized by the fact that DNA sections representing PKS that produce specifically for PUFA (polyunsaturated fatty acids) are reproduced in vitro. The PUFA PKS-specific amino acid sequence LGIDSIKRVEIL makes it possible to derive oligonucleotides which are used for the efficient PCR screening for PUFA PKS genes or PUFA-producing microorganisms. The inventive method is particularly suitable for the high throughput screening of microorganisms for PUFA PKS genes.
    • 本发明涉及用于快速和简单地鉴定微生物中PUFA PKS的方法。 所述方法的特征在于代表PKS产生的专门用于PUFA(多不饱和脂肪酸)的DNA片段在体外再现。 PUFA PKS特异性氨基酸序列LGIDSIKRVEIL可以获得用于有效PCR筛选PUFA PKS基因或产生PUFA的微生物的寡核苷酸。 本发明的方法特别适合用于PUFA PKS基因的微生物的高通量筛选。