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1. 发明授权

US6060242A PNA diagnostic methods 失效
标题翻译： PNA诊断方法
公开(公告)号：US6060242A
公开(公告)日：2000-05-09
申请号：US870370
申请日：1997-06-06
申请人： Eileen Xiao-Feng Nie , Yuan Min Wu
发明人： Eileen Xiao-Feng Nie , Yuan Min Wu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6886 , C12Q2600/156
摘要： The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a liquid medium using a plurality of PNA probes. The method is particularly advantageous in not requiring the use of a solid support to bind the probe or the target sequence.
摘要翻译：本发明提供了使用多个PNA探针快速，经济且有效地测定和测定液体培养基中的核苷酸的方法。该方法在不需要使用固体支持物结合探针或靶序列方面是特别有利的。

2. 发明授权

US6046004A Solution hybridization of nucleic acids with antisense probes having modified backbones 失效
标题翻译：核酸与具有修饰主链的反义探针的溶液杂交
公开(公告)号：US6046004A
公开(公告)日：2000-04-04
申请号：US83410
申请日：1998-05-22
申请人： Yuan Min Wu , Eileen Xiao-Feng Nie
发明人： Yuan Min Wu , Eileen Xiao-Feng Nie
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6816
摘要： The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a fluid medium using laser induced fluorescence of antisense probes. The probes can have anionic backbones of reduced negative charge. Suitable probes can include methylphosphonate backbones. When the hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the number of mismatches between the probe and target. When the hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence and proportional to the number of mismatches between the probe and target. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
摘要翻译：本发明提供了使用反义探针的激光诱导荧光来快速，经济且有效地测序和测定流体培养基中的核苷酸的方法。探针可以具有减少负电荷的阴离子主链。合适的探针可以包括甲基膦酸酯骨架。当杂交复合物和未杂交的探针在检测之前分离时，流体测试介质的荧光强度与探针和靶之间的错配数成反比。当杂交复合物和未杂交的探针在检测之前不分离时，流体测试培养基的荧光强度与探针相对于靶序列的杂交效率成反比，并且与探针和靶之间的错配数目成比例。该方法可用于鉴定折叠的核苷酸序列中的可接近区域，以确定杂交复合物中错配对的数目，并绘制基因组。

3. 发明授权

US5846729A Assaying nucleotides in solution using a fluorescent intensity quenching effect 失效
标题翻译：使用荧光强度淬灭效应测定溶液中的核苷酸
公开(公告)号：US5846729A
公开(公告)日：1998-12-08
申请号：US886280
申请日：1997-07-01
申请人： Yuan Min Wu , Eileen Xiao-Feng Nie
发明人： Yuan Min Wu , Eileen Xiao-Feng Nie
IPC分类号： C12N15/09 , C12Q1/08 , C12Q1/68 , G01N20060101 , G01N21/64 , H01S20060101
CPC分类号： C12Q1/6832 , C12Q1/6886 , C12Q2600/156 , Y10S435/81
摘要： The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a liquid medium using laser induced fluorescence of antisense probes, including PNA probes. Fluorescent intensity of the resulting medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence. The method is particularly advantageous in not requiring separation of unhybridized probes and hybridization complexes prior to detection. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
摘要翻译：本发明提供了使用包括PNA探针的反义探针的激光诱导荧光来快速，经济且有效地测序和测定液体培养基中核苷酸的方法。所得培养基的荧光强度与探针相对于靶序列的杂交效率成反比。该方法特别有利于在检测之前不需要分离未杂交的探针和杂交复合物。该方法可用于鉴定折叠的核苷酸序列中的可接近区域，以确定杂交复合物中错配对的数目，并绘制基因组。

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