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1. 发明授权

US06255050B1 Dynamic hybridization system 失效
标题翻译：动态杂交系统
公开(公告)号：US06255050B1
公开(公告)日：2001-07-03
申请号：US09083409
申请日：1998-05-22
申请人： Eileen Xiao-Feng Nie , Yuan Min Wu
发明人： Eileen Xiao-Feng Nie , Yuan Min Wu
IPC分类号： C12Q168
CPC分类号： C12Q1/6832 , C12Q1/6834 , C12Q2565/631 , C12Q2523/32
摘要： Rapid methods and means for hybridizing DNA, RNA and analogs thereof are provided. Hybridization occurs on a partition assembly through which nucleobase-containing sequences are driven by a force, such as centrifugal force, electrophoretic force, gravitational force vacuum force and/or pressure. The unbound sequences hybridize with complementary sequences bound to the partition assembly. The force applied to drive the sequences through the partition assembly increases the rate of hybridization by increasing the rate of collisions between complementary sequences.
摘要翻译：提供了用于杂交DNA，RNA及其类似物的快速方法和手段。杂交发生在分隔组件上，其中含有核碱基的序列通过力例如离心力，电泳力，重力真空力和/或压力驱动。未结合的序列与与分隔组件结合的互补序列杂交。用于驱动序列通过分隔组件的力通过增加互补序列之间的碰撞速率来增加杂交速率。

2. 发明授权

US6060242A PNA diagnostic methods 失效
标题翻译： PNA诊断方法
公开(公告)号：US6060242A
公开(公告)日：2000-05-09
申请号：US870370
申请日：1997-06-06
申请人： Eileen Xiao-Feng Nie , Yuan Min Wu
发明人： Eileen Xiao-Feng Nie , Yuan Min Wu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6886 , C12Q2600/156
摘要： The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a liquid medium using a plurality of PNA probes. The method is particularly advantageous in not requiring the use of a solid support to bind the probe or the target sequence.
摘要翻译：本发明提供了使用多个PNA探针快速，经济且有效地测定和测定液体培养基中的核苷酸的方法。该方法在不需要使用固体支持物结合探针或靶序列方面是特别有利的。

3. 发明授权

US6046004A Solution hybridization of nucleic acids with antisense probes having modified backbones 失效
标题翻译：核酸与具有修饰主链的反义探针的溶液杂交
公开(公告)号：US6046004A
公开(公告)日：2000-04-04
申请号：US83410
申请日：1998-05-22
申请人： Yuan Min Wu , Eileen Xiao-Feng Nie
发明人： Yuan Min Wu , Eileen Xiao-Feng Nie
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6816
摘要： The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a fluid medium using laser induced fluorescence of antisense probes. The probes can have anionic backbones of reduced negative charge. Suitable probes can include methylphosphonate backbones. When the hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the number of mismatches between the probe and target. When the hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence and proportional to the number of mismatches between the probe and target. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
摘要翻译：本发明提供了使用反义探针的激光诱导荧光来快速，经济且有效地测序和测定流体培养基中的核苷酸的方法。探针可以具有减少负电荷的阴离子主链。合适的探针可以包括甲基膦酸酯骨架。当杂交复合物和未杂交的探针在检测之前分离时，流体测试介质的荧光强度与探针和靶之间的错配数成反比。当杂交复合物和未杂交的探针在检测之前不分离时，流体测试培养基的荧光强度与探针相对于靶序列的杂交效率成反比，并且与探针和靶之间的错配数目成比例。该方法可用于鉴定折叠的核苷酸序列中的可接近区域，以确定杂交复合物中错配对的数目，并绘制基因组。

4. 发明授权

US5846729A Assaying nucleotides in solution using a fluorescent intensity quenching effect 失效
标题翻译：使用荧光强度淬灭效应测定溶液中的核苷酸
公开(公告)号：US5846729A
公开(公告)日：1998-12-08
申请号：US886280
申请日：1997-07-01
申请人： Yuan Min Wu , Eileen Xiao-Feng Nie
发明人： Yuan Min Wu , Eileen Xiao-Feng Nie
IPC分类号： C12N15/09 , C12Q1/08 , C12Q1/68 , G01N20060101 , G01N21/64 , H01S20060101
CPC分类号： C12Q1/6832 , C12Q1/6886 , C12Q2600/156 , Y10S435/81
摘要： The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a liquid medium using laser induced fluorescence of antisense probes, including PNA probes. Fluorescent intensity of the resulting medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence. The method is particularly advantageous in not requiring separation of unhybridized probes and hybridization complexes prior to detection. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
摘要翻译：本发明提供了使用包括PNA探针的反义探针的激光诱导荧光来快速，经济且有效地测序和测定液体培养基中核苷酸的方法。所得培养基的荧光强度与探针相对于靶序列的杂交效率成反比。该方法特别有利于在检测之前不需要分离未杂交的探针和杂交复合物。该方法可用于鉴定折叠的核苷酸序列中的可接近区域，以确定杂交复合物中错配对的数目，并绘制基因组。

5. 发明授权

US06251591B1 Quantitative method for detecting nucleotide concentration 失效
标题翻译：检测核苷酸浓度的定量方法
公开(公告)号：US06251591B1
公开(公告)日：2001-06-26
申请号：US09083837
申请日：1998-05-22
申请人： Yuan Min Wu , Eileen Xiao-Feng Nie
发明人： Yuan Min Wu , Eileen Xiao-Feng Nie
IPC分类号： C12Q168
CPC分类号： C12Q1/6813 , C12Q2527/143 , C12Q2525/113
摘要： The invention provides a method for rapidly, economically and efficiently determining the concentration of a target nucleobase-containing sequence in a fluid medium using laser induced fluorescence of antisense probes. When hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the test medium is proportional to the concentration of the target sequence. When hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the test medium is inversely proportional to the concentration of the target sequence. The method can be used to determine the concentration of a contaminant in a sample as a part of a system of quality control.
摘要翻译：本发明提供了使用反射探针的激光诱导荧光来快速，经济且有效地测定流体介质中靶核苷酸序列的浓度的方法。当杂交复合物和未杂交的探针在检测前分离时，测试培养基的荧光强度与靶序列的浓度成比例。当杂交复合物和未杂交的探针在检测之前未分离时，测试培养基的荧光强度与靶序列的浓度成反比。该方法可用于确定样品中污染物的浓度作为质量控制系统的一部分。

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