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1. 发明申请

US20030032017A1 Quantification of low molecular weight and low abundance proteins using high resolution two-dimensional electrophoresis and mass spectrometry 审中-公开
标题翻译：使用高分辨率二维电泳和质谱法定量低分子量和低丰度蛋白质
公开(公告)号：US20030032017A1
公开(公告)日：2003-02-13
申请号：US09921004
申请日：2001-08-03
申请人： Large Scale Proteomics, Corp.
发明人： Norman G. Anderson , Madhu Mondal , Rembert Pieper
IPC分类号： C12Q001/68
CPC分类号： G01N27/44773
摘要： Methods are disclosed comprising specific technologies including a system for routinely concentrating proteins from human urine ranging down to approximately 2.5 kDa automated systems for immunosubtraction of major proteins form urine and plasma to reveal minor ones, and systems for routinely fractionating protein mixtures on the basis of native molecular weight, isoelectric point that are applicable to a range of human body fluid proteins, particularly those found in urine.
摘要翻译：公开了包括特定技术的方法，包括用于常规浓缩来自人尿的蛋白质的系统，该系统从低至约2.5kDa的自动化系统免疫沉积主要蛋白质形成尿液和血浆以显示次要的蛋白质，以及基于天然存在的常规分级蛋白质混合物的系统分子量，等电点，适用于一系列人体液蛋白，特别是在尿液中发现的那些。

2. 发明申请

US20020146832A1 Automated apparatus for separating a biological sample from a two dimensional electrophoresis gel 失效
标题翻译：用于从二维电泳凝胶分离生物样品的自动装置
公开(公告)号：US20020146832A1
公开(公告)日：2002-10-10
申请号：US09859664
申请日：2001-05-18
申请人： Large Scale Proteomics Corp.
发明人： Samuel Michel , Jack Goodman , N. Leigh Anderson
IPC分类号： G01N001/06
CPC分类号： G01N27/44739 , G01N2001/288 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： An automated high-throughput system for excising spots or samples from an electrophoresis slab gel includes a computer controlled robotic arm assembly and a sample plate handling assembly for supplying a sample plate to a loading station. The computer is connected to a scanner and imaging device to identify selected sample locations on the slab gel and to direct the robotic arm to the selected locations for excising the gel spots. The cutting assembly includes a removable tray for supporting the slab gel during the cutting process and is coupled to the automated sample plate handling assembly. The sample plate handling assembly delivers a multiwell plate to the cutting assembly for receiving the gel spots. The removable tray cooperates with a scanner for identifying protein spots and includes a positioning device to position the tray in the scanner and the cutting assembly in selected locations to coordinate the scanned image with the cutting process.
摘要翻译：用于从电泳板凝胶中切割点或样品的自动化高通量系统包括计算机控制的机器人臂组件和用于将样品板供应到装载站的样品板处理组件。计算机连接到扫描仪和成像装置，以识别板状凝胶上的选定样品位置，并将机器人手臂引导到所选择的位置以切除凝胶点。切割组件包括用于在切割过程期间支撑板状凝胶的可移除托盘，并且联接到自动化样品板处理组件。样品板处理组件将多孔板输送到切割组件以接收凝胶点。可移除托盘与扫描器配合用于识别蛋白质斑点，并且包括定位装置，用于将扫描器中的托盘和切割组件定位在选定的位置，以便与切割过程协调扫描图像。

3. 发明授权

US06980674B2 Reference database 失效
公开(公告)号：US06980674B2
公开(公告)日：2005-12-27
申请号：US09753678
申请日：2001-01-04
申请人： Norman G. Anderson , N. Leigh Anderson
发明人： Norman G. Anderson , N. Leigh Anderson
IPC分类号： G06F19/00 , G06K9/00
CPC分类号： G06F19/28 , G01N33/6803 , G01N2800/52 , G06K9/00127 , Y10T436/105831
摘要： Data acquisition and cataloging are used to classify polypeptides into a reference index or database. The database can be used to identify previously unidentified samples. New polypeptides are characterized and added to the database.

4. 发明申请

US20010027920A1 Device and method for manipulation of an electrophoresis gel 审中-公开
标题翻译：用于电泳凝胶操作的装置和方法
公开(公告)号：US20010027920A1
公开(公告)日：2001-10-11
申请号：US09783136
申请日：2001-02-15
申请人： Large Scale Proteomics Corp.
发明人： N. Leigh Anderson , Jack Goodman , Andrew McGrath
IPC分类号： G01N027/26
CPC分类号： G01N27/44704
摘要： A method and device for transferring an electrophoresis gel from a cassette to a carrier includes a tray having a dimension to receive a cassette and a carrier device. The tray has a bottom wall with a surface with channels or recesses formed by projections to reduce contact of an electrophoresis gel with the bottom wall of the tray. The projections are typically pyramid-shaped members extending to a peak having a small surface area to prevent the electrophoresis gel from adhering to the bottom wall. The bottom wall also includes a recessed area to receive the carrier so that the gel can slide easily from the cassette to open jaws of the carrier. A retaining arm extends from a side wall of the tray to hold the jaws of the carrier in an open position while the gel is being positioned between the clamping surfaces of the jaws.
摘要翻译：用于将电泳凝胶从盒转移到载体的方法和装置包括具有接收盒的尺寸的托盘和载体装置。托盘具有底壁，其具有由突起形成的通道或凹部的表面，以减少电泳凝胶与托盘底壁的接触。突起通常是延伸到具有小表面积的峰的金字塔形构件，以防止电泳凝胶粘附到底壁。底壁还包括用于接纳载体的凹陷区域，使得凝胶可以从盒子容易地滑动以打开载体的钳口。保持臂从托盘的侧壁延伸以将托架的夹爪保持在打开位置，同时凝胶位于夹爪的夹紧表面之间。

5. 发明授权

US06846635B1 Microarrays and their manufacture 失效
标题翻译：微阵列及其制造
公开(公告)号：US06846635B1
公开(公告)日：2005-01-25
申请号：US09628339
申请日：2000-07-28
申请人： Norman G. Anderson , N. Leigh Anderson , James A. Braatz
发明人： Norman G. Anderson , N. Leigh Anderson , James A. Braatz
IPC分类号： G01N31/22 , B01J19/00 , B01L3/00 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/00 , C12Q1/02 , C12Q1/68 , C40B40/06 , C40B40/12 , C40B70/00 , G01N27/447 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/566 , G01N37/00
CPC分类号： B01L3/50857 , B01J19/0046 , B01J2219/00515 , B01J2219/0052 , B01J2219/00524 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00664 , B01J2219/00673 , B01J2219/00707 , B01J2219/0072 , B01J2219/00722 , B01J2219/00731 , C12Q1/6837 , C40B40/06 , C40B40/12 , C40B70/00 , G01N33/5436 , G01N33/54366 , G01N2500/00 , Y10S435/808 , Y10S436/805 , Y10S436/809 , Y10T442/20
摘要： Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that is glued to a backing.
摘要翻译：通过对于微阵列中使用的每种化合物使用单独的纤维来制备微阵列。将纤维捆扎并切片以形成胶合到背衬的薄微阵列。

6. 发明申请

US20020027078A1 Method and apparatus for unloading gels from an isoelectric gel tube onto a gel slab 审中-公开
标题翻译：将凝胶从等电凝胶管卸载到凝胶板上的方法和装置
公开(公告)号：US20020027078A1
公开(公告)日：2002-03-07
申请号：US09942986
申请日：2001-08-31
申请人： Large Scale Proteomics Corp.
发明人： N. Leigh Anderson , Jack Goodman , L. Eric Wallgren
IPC分类号： G01N027/26 , G01N027/447
CPC分类号： G01N27/44773 , G01N27/44743 , G01N27/44795
摘要： An apparatus for expressing and unloading an isoelectric focusing gel from an electrophoresis gel tube includes a first support for supporting the gel tube, a plunger rod and a second support for supporting the plunger rod. The first support is mounted on a movable carriage and is moved toward the second support so that the gel tube slides onto the plunger rod to unload the gel from the gel tube. A plurality of gel tubes can be mounted in a rack and the rack coupled to the first support. The first support preferably includes a plurality of openings oriented with the gel tubes for guiding a respective plunger rod through the axial passage of the gel tubes. In preferred embodiments, the second support supporting the plunger rods is substantially stationary while the first support moves toward the second support so that the gel tubes slide onto the plunger rods. A plunger member such as a rubber ball is positioned in the axial passage of the gel tubes between the gel and the plunger rod to unload the gel in a substantially uniform manner without tearing or breaking the gel. Guide members can be positioned on the top edge of the gel slabs for guiding the gel from the gel tube between the plates that support the gel slab. The guide members include inclined guiding surfaces forming a V-shaped trough above the edge of the gel slab.
摘要翻译：用于从电泳凝胶管表达和卸载等电聚焦凝胶的装置包括用于支撑凝胶管的第一支撑件，柱塞杆和用于支撑柱塞杆的第二支撑件。第一支撑件安装在可移动的托架上并且朝向第二支撑件移动，使得凝胶管滑动到柱塞杆上以从凝胶管卸载凝胶。多个凝胶管可以安装在机架中，并且齿条联接到第一支撑件。优选地，所述第一支撑件包括多个开口，所述多个开口通过所述凝胶管定向，用于通过所述凝胶管的轴向通道引导相应的柱塞杆。在优选实施例中，支撑柱塞杆的第二支撑件基本上是静止的，而第一支撑件朝向第二支撑件移动，使得凝胶管滑动到柱塞杆上。诸如橡胶球的柱塞构件定位在凝胶管在凝胶和柱塞杆之间的轴向通道中，以大致均匀的方式卸载凝胶，而不会撕裂或破坏凝胶。引导构件可以位于凝胶板的顶部边缘上，用于将凝胶从凝胶管引导到支撑凝胶板的板之间。引导构件包括在凝胶板的边缘上方形成V形槽的倾斜引导表面。

7. 发明申请

US20020008033A1 Automated apparatus including a robotic arm for loading samples into wells for first dimension electrophoresis separation 失效
标题翻译：自动装置包括用于将样品装入孔中的机器人手臂用于第一维电泳分离
公开(公告)号：US20020008033A1
公开(公告)日：2002-01-24
申请号：US09801831
申请日：2001-03-09
申请人： Large Scale Proteomics Corp.
发明人： Andrew McGrath , N. Leigh Anderson , Jack Goodman
IPC分类号： G01N027/26 , G01N027/447
CPC分类号： G01N27/44795 , G01N27/44743 , G01N27/44782 , G01N35/10 , Y10T436/11
摘要： An automated assembly for carrying out a first dimension electrophoresis separation of proteins and other macromolecules includes a supply magazine, an automated transferring device and an electrophoresis tank. The tank includes a rack supporting a plurality of gel tubes and includes a chamber for containing a buffer solution in contact with one end of the gel tubes. The assembly includes a planar cover member overlying the tank. The cover member has a plurality of apertures with a guide surface for guiding the pipette through the chamber to the top end of the gel tubes. The supply magazine includes a carousel for storing sample containers, a bar code reader, a holding device and an arm for transferring the sample container from the carousel to the bar code reader and the holding device. The transferring device includes a robotic assembly that is movable in three dimensions for removing a sample from a sample container and delivering the sample to a selected gel tube in the electrophoresis tank.
摘要翻译：用于进行蛋白质和其他大分子的第一维电泳分离的自动化组件包括供应盒，自动转印装置和电泳槽。容器包括支撑多个凝胶管的支架，并且包括用于容纳与凝胶管的一端接触的缓冲溶液的室。该组件包括覆盖该罐的平面覆盖件。盖构件具有多个具有引导表面的孔，用于将移液管引导通过腔室到凝胶管的顶端。供应盒包括用于存储样品容器的转盘，条形码阅读器，保持装置和用于将样品容器从转盘转移到条形码阅读器和保持装置的臂。转移装置包括可以三维移动的机器人组件，用于从样品容器中取出样品并将样品输送到电泳槽中选定的凝胶管。

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