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1. 发明申请

US20220386592A1 COMPOSITION FOR CRYOPRESERVATION OF BOVINE REPRODUCTIVE CELLS AND CRYOPRESERVATION METHOD THEREOF 有权
公开(公告)号：US20220386592A1
公开(公告)日：2022-12-08
申请号：US17881655
申请日：2022-08-05
申请人： BioVerde Inc.
发明人： Chikara Kubota , Takaaki Ando , Suong-Hyu Hyon , Woogi Hyon , Shuji Imamura , Satoru Gessei
IPC分类号： A01N1/02
摘要： Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w/w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is ε-poly-L-lysine having a number average molecular weight of 1,000 to 20,000 wherein 50 to 99 mol % of amino groups of the ε-poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and 2 to 4 w/w % of glycerol, as dissolved in a physiological solution. A preferred embodiment of the cryopreservation method comprises steps of: primary diluting, in which bovine semen is diluted to 2.5 to 10 times with a physiological solution and kept at 2 to 8° C.; and secondary diluting, in which the bovine semen is diluted to 5 to 20 times while being kept at 2 to 8° C., by adding dropwise a physiological solution containing the amphoteric polyelectrolyte (antifreeze polyamino acid) and glycerol to a suspension obtained in the primary diluting.

2. 发明授权

US11659833B2 Composition for cryopreservation of bovine reproductive cells and cryopreservation method thereof 有权
公开(公告)号：US11659833B2
公开(公告)日：2023-05-30
申请号：US16326978
申请日：2017-08-22
申请人： BioVerde Inc.
发明人： Chikara Kubota , Takaaki Ando , Suong-Hyu Hyon , Woogi Hyon , Shuji Imamura , Satoru Gessei
IPC分类号： A01N1/02
CPC分类号： A01N1/0221 , A01N1/02 , A01N1/0226 , A01N1/0231 , A01N1/0284
摘要： Provided are a vehicle body assembling method and a vehicle body assembling apparatus which allow a simple configuration in the vicinity of the connecting portion between an upper jig and a lower jig and allow an increase in the efficiency of assembling work (welding work). A vehicle body assembling apparatus is equipped with a jig for supporting vehicle body components in a preassembled position, the jig comprising an upper jig and a lower jig which are connected to each other in at least two places. Each of the connection places is provided with a connecting means for fixing a three-dimensional coordinate position while allowing uniaxial turning. The vehicle body assembling apparatus is also equipped with a conveying means for conveying the upper jig which supports the vehicle body components, and reduces the load applied to the lower jig from the upper jig when connecting the upper jig to the lower jig.

3. 发明申请

US20070259327A1 Cryopreservation medium and cryopreservation method for tissues and cells 审中-公开
标题翻译：冷冻保存培养基和组织和细胞的低温保存方法
公开(公告)号：US20070259327A1
公开(公告)日：2007-11-08
申请号：US11418421
申请日：2006-05-03
申请人： Yasuhiro Iwanaga , Kazuaki Matsumura , Jong-Yoon Kim , Hajime Sugai , Suong-Hyu Hyon
发明人： Yasuhiro Iwanaga , Kazuaki Matsumura , Jong-Yoon Kim , Hajime Sugai , Suong-Hyu Hyon
IPC分类号： C12N5/08 , A01N1/02
CPC分类号： A01N1/02 , A01N1/0221
摘要： A cryopreservation medium and a cryopreservation method which make it possible to cryopreserve tissues and cells with high viability are provided. A cryopreservation medium contains polyphenol at a concentration of from 30 to 120 ppm. In an embodiment, a cryopreservation method for tissues and cells comprises: incubating tissues or cells for 2 hours in culture medium supplemented with polyphenol at concentrations of from 30 to 120 ppm; suspending tissues or cells in a cryopreservation medium; freezing the suspension of the tissues or cells in the cryopreservation medium; and storing the frozen suspension of tissues or cells in the cryopreservation medium.
摘要翻译：提供了一种冷冻保存培养基和一种低温保存方法，其可以以高活力冷冻保存组织和细胞。冷冻保存培养基含有浓度为30至120ppm的多酚。在一个实施方案中，用于组织和细胞的冷冻保存方法包括：在补充有30至120ppm浓度的多酚的培养基中孵育组织或细胞2小时; 将组织或细胞悬浮在冷冻保存培养基中; 冻结冷冻保存培养基中组织或细胞的悬浮液; 并将冷冻的组织或细胞悬浮液储存在冷冻保存培养基中。

4. 发明公开

US20230189790A1 NON-FREEZING REFRIGERATED STORAGE LIQUID FOR STEM CELLS 审中-公开
公开(公告)号：US20230189790A1
公开(公告)日：2023-06-22
申请号：US17768370
申请日：2020-11-05
申请人： BIOVERDE INC.
发明人： Akira AIZAWA , Suong-Hyu HYON , Woogi HYON
IPC分类号： A01N1/02
CPC分类号： A01N1/021
摘要： A non-freezing refrigerated storage liquid for stem cells such as iPS cells, according to an embodiment, comprises: potassium ion species in a range of 30 to 80 mmoL/L; sodium ion species in a range of 30 to 80 mmoL/L, wherein ion-based molar ratio of sodium ion species to potassium ion species (ratio ofNa+ to K+) is in a range of 0.5 to 1.3; trolox or its analog at a concentration of 1 to 8 mM; adenine or a salt or derivative thereof at a concentration of 0.1 to 4.2 mM; all of or all but one, two or three of essential amino acids, total content of which is 50 to 200 mg; and non-essential amino acids, total content of which is 50 to 200 mg/L.

5. 发明申请

US20070259329A1 Anergy-inducing cellular composition 审中-公开
标题翻译：抗生素诱导细胞组成
公开(公告)号：US20070259329A1
公开(公告)日：2007-11-08
申请号：US11418422
申请日：2006-05-03
申请人： Jong-Yoon Kim , Kazuaki Matsumura , Yasuhiro Iwanaga , Hajime Sugai , Suong-Hyu Hyon
发明人： Jong-Yoon Kim , Kazuaki Matsumura , Yasuhiro Iwanaga , Hajime Sugai , Suong-Hyu Hyon
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0226
摘要： The invention is directed to an anergy-inducing cellular composition comprising: cells having antigenic determinants on the cell surface and an anergy-inducing compound [e.g., (−)-epigallocatechin-3-o-gallate (EGCG)] attached by its chemical affinity to the antigenic determinants. In one embodiment, the anergy-inducing compound blocks co-stimulatory molecules among the antigenic determinants of the cells, which upon encountering with T-cell renders the latter anergic, that is, unresponsive toward alloantigens. In another embodiment, EGCG is of high purity for its efficient attachment to the antigenic determinants. The anergy-inducing cellular composition is prepared by immersing the cells in a culture media solution (RPMI 1640) containing 50-500 ppm EGCG at low physiological temperatures for one to two hours to minimize the cells' mortality. A specific use of the resulting anergic cellular composition is suggested, which is the attenuation of two undesirable acute allogenic responses resulting from major histocompatibility disparity, namely, transplant rejection and GVHD.
摘要翻译：本发明涉及一种免疫诱导细胞组合物，其包含：在细胞表面具有抗原决定簇的细胞和通过其化学亲合力附着的无能力诱导化合物[例如（ - ） - 表没食子儿茶素-3-邻氯乙酸酯（EGCG）] 对抗原决定簇。在一个实施方案中，所述无反应诱导化合物阻断所述细胞的抗原决定簇之间的共刺激分子，其在与T细胞相遇时使得后者不敏感，即对同种异体抗原无反应。在另一个实施方案中，EGCG与抗原决定簇的有效连接具有高纯度。通过将细胞浸入含有50-500ppm EGCG的培养基溶液（RPMI 1640）中在低生理温度下浸泡1至2小时来制备无能诱导细胞组合物，以使细胞的死亡率最小化。提出了所得到的无菌细胞组合物的具体用途，其是由主要组织相容性差异（即移植排斥和GVHD）引起的两种不希望的急性同种异体反应的减弱。

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