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1. 发明申请

US20210364494A1 CELL-SUBSTRATE IMPEDANCE MONITORING OF CANCER CELLS OF DIFFERENT LINEAGE, ORIGIN, OR STAGE 有权
公开(公告)号：US20210364494A1
公开(公告)日：2021-11-25
申请号：US16635911
申请日：2018-08-01
申请人： ACEA BIOSCIENCES, INC.
发明人： Nan Li , Yama Abassi , Xiao Xu , Xiaobo Wang
IPC分类号： G01N33/483 , G01N1/14 , G01N27/02
摘要： A method of assessing cytolysis of cancer cells, the method including: providing a cell substrate impedance monitoring cartridge having a plurality of chambers, each chamber having an electrode array configured for measuring cell-substrate impedance, wherein different chambers are preloaded with different target cells embodied as cancer cells of different lineage, origin or stage; adding effector cells to the plurality chambers for interaction with the target cells, wherein the effector cells are immune cells; monitoring cell-substrate impedance of the plurality of chambers before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedances; and determining effectiveness of effector cell killing of the different target cells by comparing the impedances or impedance-based parameters over time.

2. 发明授权

US10648913B2 System and method for detecting multiple-excitation-induced light in a flow channel 有权
公开(公告)号：US10648913B2
公开(公告)日：2020-05-12
申请号：US15415439
申请日：2017-01-25
申请人： ACEA BIOSCIENCES, INC
发明人： Nan Li , Jian Wu , Ye Chen , Tianxing Wang , Xiaobo Wang
IPC分类号： G01N21/64 , G01N15/14 , G01N15/10
摘要： A system for detecting signal components of light induced by multiple excitation sources, which includes a flow channel having two spatially separated optical interrogation zones; a light illumination subsystem that directs each of two light beams of different wavelengths to a different zone of the optical interrogation zones; a detector subsystem that converts detected light into a total electrical signal having both modulated and unmodulated electrical signals; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

3. 发明授权

US10620188B2 Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways 有权
公开(公告)号：US10620188B2
公开(公告)日：2020-04-14
申请号：US15612688
申请日：2017-06-02
申请人： ACEA Biosciences, Inc.
发明人： Yama A. Abassi , Li Zhao , Ning Ke , Xiaobo Wang , Xiao Xu
IPC分类号： C40B30/04 , G01N33/50 , C12Q1/00 , G01N33/543
CPC分类号： G01N33/5014 , C12Q1/002 , G01N33/5011 , G01N33/5041 , G01N33/5438 , C40B30/04 , G01N2333/4716
摘要： Methods of identifying whether a biologically active agent affects an oncogene addicted pathway within a cancer cell, by introducing a biologically active agent suspected of affecting an oncogene addicted pathway to a first well and a negative control to a second well, and introducing a stimulating agent that stimulates the oncogene addicted pathway to both wells; monitoring cell-substrate impedance of the two wells and optionally determining cell indices from impedance values; generating an impedance based curve for each of the two wells from the impedance values or from the cell indices; comparing the impedance-based curves to determine a degree of similarity; and if significantly different concluding the biologically active agent affects the oncogene addicted pathway within the cancer cells.

4. 发明授权

US10551371B2 System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function 有权
公开(公告)号：US10551371B2
公开(公告)日：2020-02-04
申请号：US12774709
申请日：2010-05-05
申请人： Xiaobo Wang , Wei Ouyang , Nan Li , Biao Xi , Yama A. Abassi , Xiao Xu
发明人： Xiaobo Wang , Wei Ouyang , Nan Li , Biao Xi , Yama A. Abassi , Xiao Xu
IPC分类号： G01N33/487
摘要： Devices and methods for performing extracellular recording of cells, such as excitable cells, cardiomyocytes, and cardiomyocyte precursor cells is provided. An exemplary device includes a nonconductive substrate forming or provided as a base of one or more wells; a recording electrode positioned on the substrate within the well, wherein the recording electrode is accessible to cells when a cell sample is added to the device; and a reference electrode positioned within the well in a cell-free zone, the cell-free zone characterized as free from contact with cells when the cell sample is added to the device, thereby preventing contact between cells and the reference electrode.

5. 发明授权

US10018581B2 Impedance based devices and methods for use in assays 有权
公开(公告)号：US10018581B2
公开(公告)日：2018-07-10
申请号：US13802174
申请日：2013-03-13
申请人： ACEA BIOSCIENCES, INC
发明人： Xiaobo Wang , Xiao Xu
IPC分类号： B01L3/00 , G01N27/02 , C12M1/32 , C12M1/34 , G01N27/327
CPC分类号： G01N27/02 , C12M23/12 , C12M41/36 , G01N27/3276 , Y10T29/49117
摘要： A method for assaying target molecules in a sample liquid, the method comprising: providing an impedance monitoring device operably connected to an impedance analyzer; adding a sample liquid suspected of having target molecules to the well thereby permitting binding of target molecules to the capture molecules; monitoring impedance of the well; and determining the presence, amount or concentration of target molecules in the liquid sample from the monitored impedance. The device includes a nonconducting substrate having a well, at least two electrodes fabricated on a bottom of the well and on a same plane, wherein the surfaces of the at least two electrodes are modified with capture molecules configured to bind target molecules in a liquid sample, and at least two connection pads electrically connected to the at least two electrodes.

6. 发明授权

US09568423B2 System and method for detecting multiple-excitation-induced light in a flow channel 有权
标题翻译：用于检测流道中多激发光的系统和方法
公开(公告)号：US09568423B2
公开(公告)日：2017-02-14
申请号：US13657845
申请日：2012-10-22
申请人： ACEA BIOSCIENCES, INC
发明人： Nan Li , Jian Wu , Ye Chen , Tianxing Wang , Xiaobo Wang
IPC分类号： G01N21/64 , G01F1/00 , G01N15/14 , G01N15/10
CPC分类号： G01N21/645 , G01N15/1429 , G01N15/1434 , G01N15/1459 , G01N21/64 , G01N21/6428 , G01N2015/1006 , G01N2015/1438 , G01N2015/149 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2021/6482 , G01N2201/06113 , G01N2201/0621
摘要： A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.
摘要翻译：一种用于检测由多个激发源引起的光的信号分量的系统，包括：流路，包括至少两个空间分离的光询问区; 非调制激励源，其将第一波长的光束以接近恒定的强度引导到第一光学询问区上; 调制激励源，其将以调制频率随时间调制的第二波长的光束引导到第二光学询问区上; 检测器子系统，包括一组检测器，其被配置为检测从流过所述至少两个光询问区的颗粒发射的光并将检测到的光转换成总电信号; 以及处理器，其根据从每个光询问区检测的光来确定信号分量。

7. 发明授权

US08916357B2 Dynamic monitoring of activation of receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology 有权
标题翻译：使用实时微电子传感技术动态监测活细胞中受体酪氨酸激酶（RTK）的活化
公开(公告)号：US08916357B2
公开(公告)日：2014-12-23
申请号：US13569156
申请日：2012-08-07
申请人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
发明人： Yama A. Abassi , Naichen Yu , Josephine Atienza , Xiao Xu , Xiaobo Wang
IPC分类号： C12Q1/37 , G01N33/50 , C12Q1/00 , G01N33/543 , C12M1/42
CPC分类号： G01N33/5041 , C12Q1/002 , G01N27/021 , G01N33/5014 , G01N33/5438 , G01N2333/4716 , G01N2333/705 , G01N2333/91205 , G01N2500/04 , G01N2500/10
摘要： Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control to at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance.
摘要翻译：用于鉴定能够与受体酪氨酸激酶（RTK）相互作用的化合物的方法，包括提供能够测量细胞 - 基底阻抗的装置，将表达RTK的测试细胞加入到装置中，测量第一阻抗，将化合物加到至少一种化合物并且向至少一个控制井添加车辆控制，测量化合物井和对照井的第二阻抗，确定化合物井和一个对照井的阻抗变化，比较化合物井之间的阻抗变化和控制井，并且如果比较显示阻抗变化之间的显着差异，则识别化合物与RTK相互作用。

8. 发明申请

US20140057283A1 IMPEDANCE BASED DEVICES AND METHODS FOR USE IN ASSAYS 有权
标题翻译：基于阻抗的设备和用于测量的方法
公开(公告)号：US20140057283A1
公开(公告)日：2014-02-27
申请号：US13802174
申请日：2013-03-13
申请人： ACEA BIOSCIENCES, INC
发明人： Xiaobo Wang , Xiao Xu
IPC分类号： G01N27/02
CPC分类号： G01N27/02 , C12M23/12 , C12M41/36 , G01N27/3276 , Y10T29/49117
摘要： A method for assaying target molecules in a sample liquid, the method comprising: providing an impedance monitoring device operably connected to an impedance analyzer; adding a sample liquid suspected of having target molecules to the well thereby permitting binding of target molecules to the capture molecules; monitoring impedance of the well; and determining the presence, amount or concentration of target molecules in the liquid sample from the monitored impedance. The device includes a nonconducting substrate having a well, at least two electrodes fabricated on a bottom of the well and on a same plane, wherein the surfaces of the at least two electrodes are modified with capture molecules configured to bind target molecules in a liquid sample, and at least two connection pads electrically connected to the at least two electrodes.
摘要翻译：一种用于测定样品液体中的靶分子的方法，所述方法包括：提供可操作地连接到阻抗分析仪的阻抗监测装置; 将怀疑具有靶分子的样品液体加入到孔中，从而允许靶分子与捕获分子的结合; 监测井的阻抗; 以及从所监测的阻抗确定液体样品中靶分子的存在，量或浓度。该器件包括具有阱的非导电衬底，至少两个电极，其制造在阱的底部并且在同一平面上，其中至少两个电极的表面被配置成结合在液体样品中的靶分子结合的俘获分子以及至少两个电连接到所述至少两个电极的连接焊盘。

9. 发明授权

US08206903B2 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses 有权
标题翻译：分子进入细胞的基于电穿孔的装置和方法以及细胞反应的动态监测
公开(公告)号：US08206903B2
公开(公告)日：2012-06-26
申请号：US11286882
申请日：2005-11-23
申请人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
发明人： Xiaobo Wang , Yama A. Abassi , Josephine Atienza , Xiao Xu , Junquan Xu
IPC分类号： C12Q1/68
CPC分类号： C12N15/87 , C12M35/02 , G01N15/12 , G01N33/48728
摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
摘要翻译：本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

10. 发明授权

US07876108B2 Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays 有权
标题翻译：实时电子细胞感测系统和细胞毒性分析和化合物分析的应用
公开(公告)号：US07876108B2
公开(公告)日：2011-01-25
申请号：US12480592
申请日：2009-06-08
申请人： Yama A. Abassi , Xiaobo Wang , Xiao Xu
发明人： Yama A. Abassi , Xiaobo Wang , Xiao Xu
IPC分类号： G01R27/08 , C12M1/34 , C12M3/00
CPC分类号： C12Q1/025 , C12Q1/002 , G01N27/02 , G01N33/4836 , G01N33/5014 , G01N33/5438
摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.
摘要翻译：本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件用于控制设备站和阻抗分析仪。在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。该方法可用于测试化合物对细胞的影响，例如细胞毒性测定。还提供了化合物的细胞毒性分析方法。

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