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1. 发明申请

US20170020939A1 IMMUNE BALANCE REGULATOR 审中-公开
标题翻译：免费平衡调节器
公开(公告)号：US20170020939A1
公开(公告)日：2017-01-26
申请号：US15302300
申请日：2015-04-08
申请人： euglena Co., Ltd.
发明人： Ayaka NAKASHIMA , Yuta ASAYAMA , Eriko YOSHIDA , Osamu IWATA , Kengo SUZUKI
IPC分类号： A61K36/02 , A23L33/10 , A61K31/715
CPC分类号： A61K36/02 , A23L33/10 , A23L33/105 , A23V2002/00 , A61K31/715 , A61K31/716 , C12N1/10
摘要： The present invention provides a novel immune balance regulator that regulates an immune balance in a living body. The immune balance regulator contains a Euglena-derived material and regulates an immune balance between Th1, Th2, and Th17, which is a balance between immune responses individually induced by Th1, Th2, and Th17 in a living body. The immune balance regulator regulates the immune balance between Th1, Th2, and Th17 so that immune responses induced by Th1 become relatively dominant over immune responses induced by Th2 or Th17 to improve a physical constitution that has an immune imbalance of Th1, Th2, and Th17 shifted towards Th2 and to prevent or treat a disease associated with the immune imbalance of Th1, Th2, and Th17 shifted towards a Th2 dominance. The immune balance regulator is administered prior to expected onset of a disease associated with the immune imbalance of Th1, Th2, and Th17 shifted towards the Th2 dominance.
摘要翻译：本发明提供了调节活体免疫平衡的新型免疫平衡调节剂。免疫平衡调节物含有来自Euglena的材料并调节Th1，Th2和Th17之间的免疫平衡，Th1，Th2和Th17是活体中Th1，Th2和Th17单独诱导的免疫反应之间的平衡。免疫平衡调节因子调节Th1，Th2和Th17之间的免疫平衡，使Th1诱导的免疫反应相对于由Th2或Th17诱导的免疫应答相对优势，以改善具有Th1，Th2和Th17免疫失衡的物理结构转移到Th2，并预防或治疗与Th1，Th2和Th17的免疫不平衡相关的疾病向Th2优势转移。免疫平衡调节剂在预期发生与Th1，Th2和Th17的免疫失衡相关的疾病之前施用，向Th2优势移动。

2. 发明申请

US20170319632A1 PROPHYLACTIC OR THERAPEUTIC AGENT FOR PEPTIC ULCER, FOOD ADDITIVE FOR PROPHYLACTIC OR THERAPEUTIC USE, INOS EXPRESSION INHIBITOR AND COX-2 EXPRESSION INHIBITOR 审中-公开
公开(公告)号：US20170319632A1
公开(公告)日：2017-11-09
申请号：US15524522
申请日：2015-11-06
申请人： euglena Co., Ltd.
发明人： Eriko YOSHIDA , Yuta ASAYAMA , Osamu IWATA , Ayaka NAKASHIMA , Kengo SUZUKI , Misa OHGUSHI , Naoki HARADA , Yoshihisa NAKANO
IPC分类号： A61K35/68 , A61K31/716
CPC分类号： A61K35/68 , A61K31/716
摘要： Provided are a prophylactic or therapeutic agent for a peptic ulcer, a food additive for prevention and treatment of a peptic ulcer, an iNOS expression inhibitor, and a COX-2 expression inhibitor, all of which have little side effects. The prophylactic or therapeutic agent contains a Euglena-derived material as an active ingredient. The Euglena-derived material may be Euglena, paramylon, or an acid-treated paramylon. The prophylactic or therapeutic agent is useful for the prevention or treatment of a peptic ulcer induced by psychological stress. The peptic ulcer may be a gastric ulcer. The food additive contains a Euglena-derived material as an active ingredient.

3. 发明申请

US20160010070A1 METHOD FOR INTRODUCING GENE TO EUGLENA, AND TRANSFORMANT THEREFROM 审中-公开
标题翻译：将基因引入EUGLENA及其变体的方法
公开(公告)号：US20160010070A1
公开(公告)日：2016-01-14
申请号：US14770715
申请日：2014-02-28
申请人： EUGLENA CO., LTD. , KINKI UNIVERSITY
发明人： Shigeru SHIGEOKA , Masahiro TAMOI , Kengo SUZUKI , Eriko YOSHIDA
IPC分类号： C12N9/16 , C12N15/79
CPC分类号： C12N9/16 , C12N15/79 , C12N15/8201 , C12N15/8207 , C12N15/8245 , C12N15/8246 , C12N15/825 , C12N15/8261 , C12N15/8269 , C12N15/895 , C12Y301/03011 , C12Y301/03037 , Y02A40/146
摘要： The present invention provides a method for introducing a gene into Euglena, which can stably maintain a foreign gene, and a transformant therefrom. In this method of introducing a gene into Euglena, a DNA fragment containing an amino acid sequence for encoding a protein is introduced into a Euglena cell. The method includes a step of producing a binary vector containing a DNA fragment, a step of obtaining a linear gene fragment that includes a T-DNA region including the DNA fragment in the binary vector, and a direct gene introduction step of directly introducing the linear gene fragment into the Euglena cell.
摘要翻译：本发明提供了将基因引入到可以稳定地维持外源基因的金丝雀中的方法及其转化体。在将基因引入欧氏细胞膜的方法中，将含有用于编码蛋白质的氨基酸序列的DNA片段引入欧格琳娜细胞。该方法包括制备含有DNA片段的二元载体的步骤，获得包含二元载体中包含DNA片段的T-DNA区的线性基因片段的步骤和直接引入线性的直接基因导入步骤基因片段进入Euglena细胞。

4. 发明申请

US20170037360A1 ALGAE CULTIVATION METHOD AND PRODUCTION METHOD FOR OSMOTIC PRESSURE REGULATOR 审中-公开
标题翻译：用于压力调节器的ALGAE生产方法和生产方法
公开(公告)号：US20170037360A1
公开(公告)日：2017-02-09
申请号：US15304601
申请日：2015-04-16
申请人： EUGLENA CO., LTD. , SHIMIZU CORPORATION
发明人： Kengo SUZUKI , Ryohei NAKANO , Hideyuki ADACHI , Ayaka NAKASHIMA , Eriko YOSHIDA , Masaharu TASAKI , Yoichi KUROIWA , Keisuke KOJIMA , Takeshi HASEGAWA
IPC分类号： C12N1/12 , C12P13/04 , C12P19/44
CPC分类号： C12N1/12 , C12N1/20 , C12P13/001 , C12P13/04 , C12P19/44
摘要： An algae culture method capable of efficiently producing an osmotic pressure adjusting substance, a production method of the substance, and an algae culture method for recovering carbon dioxide from a mixed gas containing carbon dioxide and sulfurous acid gas. The methods involve preparing a plurality of enrichment cultures each containing betaine by culturing a culture of microalgae derived from an environmental specimen under a photoautotrophic condition and under a plurality of culture conditions; making a cultivation plan in which an optimum enrichment culture suitable for a main culture is selected from the plurality of enrichment cultures; producing a main culture that contains betaine under the photoautotrophic condition and a salt concentration of 10 wt. % or more; and separating betaine. In the main culture, the algae containing betaine are cultured while the mixed gas containing sulfurous acid gas and carbon dioxide is blown to a culture solution of the algae.
摘要翻译：能够有效地制造渗透压调节物质的藻类培养方法，该物质的制造方法以及从含有二氧化碳和亚硫酸气体的混合气体中回收二氧化碳的藻类培养方法。所述方法包括通过在光自养条件下和多种培养条件下培养来自环境样品的微藻培养物来制备各自含有甜菜碱的多种富集培养物; 从多个富集培养物中选择适合于主要培养物的最佳富集培养物的栽培方案; 产生在光自养条件下含有甜菜碱并且盐浓度为10重量％的主要培养物。％或者更多; 并分离甜菜碱。在主要培养物中，培养含有甜菜碱的藻类，同时将含有亚硫酸气体和二氧化碳的混合气体吹入藻类的培养液中。

5. 发明申请

US20160186153A1 B-1,3-GLUCANASE, POLYNUCLEOTIDE, RECOMBINANT VECTOR, TRANSFORMANT, PRODUCTION METHOD FOR B-1,3- GLUCANASE, ENZYME PREPARATION, AND PRODUCTION METHOD FOR PARAMYLON HAVING REDUCED MOLECULAR WEIGHT 有权
标题翻译： B-1,3-GLUCANASE，POLYNUCLEOTIDE，重组载体，改造剂，B-1,3-葡萄糖苷酶的生产方法，酶的制备和具有降低的分子量的参考物的生产方法
公开(公告)号：US20160186153A1
公开(公告)日：2016-06-30
申请号：US14909211
申请日：2014-08-04
申请人： EUGLENA CO., LTD.
发明人： Kengo SUZUKI , Ryo ARASHIDA , Yuka MARUKAWA , Eriko YOSHIDA , Takumi TAKEDA , Yuki NAKANO , Naotake KONNO , Machiko TAKAHASHI
IPC分类号： C12N9/42 , C12P19/14 , C12P19/12
CPC分类号： C12N9/244 , A23K20/189 , C12N9/2405 , C12P19/12 , C12P19/14 , C12Y302/01006 , C12Y302/01039
摘要： A β-1,3-glucanase that exhibits decomposition activity with respect to paramylon derived from the genus Euglena. The β-1,3-glucanase is derived from the genus Euglena and exhibits the properties indicated below: (1) effect: hydrolyzing the β-1,3-bond of β-1,3-glucan; and (2) substrate specificity: decomposing at least paramylon. The β-1,3-glucanase additionally exhibits the properties indicated below: (3) decomposition activity: the ratio of paramylon decomposition activity with respect to laminarin decomposition activity is 20% or higher; (4) optimum pH: 3.7-7.0; and (5) optimum temperature: 30-70° C.
摘要翻译：相对于源自金丝桃属的参考亚胺的分解活性的A＆bgr-1,3-葡聚糖酶。 β-葡聚糖酶衍生自金丝桃属，表现出以下性质：（1）效果：水解β-1,3-葡聚糖的β-1,3-键; 和（2）底物特异性：至少分解paramylon。（3）分解活性：分子活性相对于昆布素分解活性的比例为20％以上;（3）分解活性：（4）最适pH：3.7-7.0; 和（5）最适温度：30-70℃

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