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2. 发明授权

US09796982B2 Use of Cry1Da in combination with Cry1Ca for management of resistant insects 有权
公开(公告)号：US09796982B2
公开(公告)日：2017-10-24
申请号：US13516663
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
IPC分类号： C12N15/82 , A01N63/02
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N65/00
摘要： The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

3. 发明申请

US20130042374A1 COMBINED USE OF Cry1Fa AND Cry1Ab PROTEINS FOR CONTROL OF 1Ab-RESISTANT SUGARCANE BORER AND FOR INSECT RESISTANCE MANAGEMENT IN SUGARCANE 审中-公开
标题翻译：联合使用Cry1Fa和Cry1Ab蛋白质控制耐药性糖尿病和耐药性管理
公开(公告)号：US20130042374A1
公开(公告)日：2013-02-14
申请号：US13516619
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Stephanie L. Burton
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Stephanie L. Burton
IPC分类号： A01H5/00
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The subject invention includes methods and sugarcane plants for controlling sugarcane borer (SCB) insects, said sugarcane plants comprising Cry1Fa and Cry1Ab core toxin containing proteins in combination to delay or prevent development of resistance by the SCB.
摘要翻译：本发明包括用于控制甘蔗螟（SCB）昆虫的方法和甘蔗植物，所述甘蔗植物包含含有Cry1Fa和Cry1Ab核心毒素的蛋白质，以延迟或预防由SCB发展的抗性。

4. 发明申请

US20120311746A1 INSECT RESISTANCE MANAGEMENT WITH COMBINATIONS OF CRY1BE AND CRY1F PROTEINS 有权
标题翻译： CRY1BE和CRY1F蛋白质组合的耐药性管理
公开(公告)号：US20120311746A1
公开(公告)日：2012-12-06
申请号：US13516629
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Stephanie L. Burton , Aaron T. Woosley
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Stephanie L. Burton , Aaron T. Woosley
IPC分类号： A01H5/00 , C12N5/10 , A01N63/00 , A01C11/00 , A01N37/18 , C12N15/82 , A01G1/00 , A01H5/10 , A01P7/04
CPC分类号： C12N15/8286 , A01N63/02 , A01N65/00 , Y02A40/162 , A01N2300/00
摘要： The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.
摘要翻译：本发明部分涉及将Cry1Be毒素与Cry1Fa毒素一起堆叠以防止昆虫本身对毒素产生抗性。如本文更详细讨论的那样，本发明的对象蛋白质是特别有利的组合，因为没有其他蛋白质对可以提供针对草地贪夜蛾（Spodoptera frugiperda）（FAW）和鸵鸟鸵鸟（Ostrinia nubilalis）的高水平的控制和非交叉抗性活性 ECB）昆虫。这种双重非交叉抗性活性也是有利的，因为它可以减少用多种非交叉抗性蛋白质靶向这些昆虫所需的蛋白质/基因的数量。这可以减少或消除避难所面积的需要。因此，本发明还通常涉及使用四个基因提供用于第一昆虫的非交叉耐药性控制的三种蛋白质和用于第二种昆虫的非交叉耐药性控制的三种蛋白质。在优选的实施方式中，目标昆虫是FAW和ECB。

5. 发明授权

US09556453B2 Insect resistance management with combinations of Cry1Be and Cry1F proteins 有权
标题翻译：昆虫抗性管理与Cry1Be和Cry1F蛋白的组合
公开(公告)号：US09556453B2
公开(公告)日：2017-01-31
申请号：US13516629
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Stephanie L. Burton , Aaron T. Woosley
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Stephanie L. Burton , Aaron T. Woosley
IPC分类号： C12N15/82 , A01N63/02 , A01N65/00
CPC分类号： C12N15/8286 , A01N63/02 , A01N65/00 , Y02A40/162 , A01N2300/00
摘要： The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.
摘要翻译：本发明部分涉及将Cry1Be毒素与Cry1Fa毒素一起堆叠以防止昆虫本身对毒素产生抗性。如本文更详细讨论的那样，本发明的对象蛋白质是特别有利的组合，因为没有其他蛋白质对可以提供针对草地贪夜蛾（Spodoptera frugiperda）（FAW）和鸵鸟鸵鸟（Ostrinia nubilalis）的高水平的控制和非交叉抗性活性 ECB）昆虫。这种双重非交叉抗性活性也是有利的，因为它可以减少用多种非交叉抗性蛋白质靶向这些昆虫所需的蛋白质/基因的数量。这可以减少或消除避难所面积的需要。因此，本发明还通常涉及使用四个基因提供用于第一昆虫的非交叉耐药性控制的三种蛋白质和用于第二种昆虫的非交叉耐药性控制的三种蛋白质。在优选的实施方式中，目标昆虫是FAW和ECB。

6. 发明授权

US08771708B2 Biologically-active radiolabeled Cry1Fa and receptor binding assay methods 有权
标题翻译：生物活性放射性标记的Cry1Fa和受体结合测定方法
公开(公告)号：US08771708B2
公开(公告)日：2014-07-08
申请号：US13327842
申请日：2011-12-16
申请人： Steven L. Evans , Jianquan Li , Joel J. Sheets
发明人： Steven L. Evans , Jianquan Li , Joel J. Sheets
IPC分类号： A61K39/07
CPC分类号： C07K14/325
摘要： Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.
摘要翻译：半胱氨酸特异性放射性标记的Cry1Fa蛋白保留对昆虫害虫的杀虫活性，并以可饱和的方式结合昆虫刷状体膜囊泡受体。生物活性放射性标记的Cry1Fa蛋白可用于与其他Cry毒素的竞争性结合测定。

7. 发明申请

US20120317681A1 COMBINED USE OF CRY1Ca AND CRY1Fa PROTEINS FOR INSECT RESISTANCE MANAGEMENT 有权
标题翻译：联合使用CRY1Ca和CRY1Fa蛋白进行耐药性管理
公开(公告)号：US20120317681A1
公开(公告)日：2012-12-13
申请号：US13516611
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
IPC分类号： A01H5/00 , C12N5/10 , C12N1/21 , C12N1/19 , A01C11/00 , A01N63/00 , A01N63/04 , A01P7/04 , A01G1/00 , A01H5/10 , C12N1/13
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N65/00 , A01N2300/00
摘要： The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).
摘要翻译：本发明包括组合用于控制鳞翅目昆虫的方法和植物，所述植物包含Cry1Fa杀虫蛋白和Cry1Ca杀虫蛋白，以延迟或预防昆虫的抗性发展。

8. 发明授权

US09045766B2 Combined use of Vip3Ab and Cry1Ab for management of resistant insects 有权
标题翻译：结合使用Vip3Ab和Cry1Ab来管理抗性昆虫
公开(公告)号：US09045766B2
公开(公告)日：2015-06-02
申请号：US13329013
申请日：2011-12-16
申请人： Thomas Meade , Kenneth Narva , Joel J. Sheets , Nicholas P. Storer , Stephanie L. Burton , Aaron T. Woosley
发明人： Thomas Meade , Kenneth Narva , Joel J. Sheets , Nicholas P. Storer , Stephanie L. Burton , Aaron T. Woosley
IPC分类号： C12N15/82 , C07K14/325 , C07K14/32
CPC分类号： C12N15/8286 , C07K14/32 , C07K14/325 , Y02A40/162
摘要： The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Vip3Ab insecticidal protein in combination with a Cry1Ab protein to delay or prevent development of resistance by the insect(s)—particularly corn earworm.
摘要翻译：本发明包括用于控制鳞翅目昆虫的方法和植物，所述植物包含与Cry1Ab蛋白结合的Vip3Ab杀虫蛋白，以延迟或防止由昆虫特别是玉米蛀虫发展的抗性。

9. 发明申请

US20130007924A1 INSECTCIDAL PROTEIN COMBINATIONS COMPRISING Cry1AB AND CRY2AA FOR CONTROLLING EUROPEAN CORN BORER, AND METHODS FOR INSECT RESISTANCE MANAGEMENT 审中-公开
标题翻译：包含Cry1AB和CRY2AA的用于控制欧洲玉米螟的INSECTCIDAL蛋白质组合，以及耐药性管理方法
公开(公告)号：US20130007924A1
公开(公告)日：2013-01-03
申请号：US13516673
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
IPC分类号： A01H5/00 , A01P7/04 , A01C11/00 , C12N5/10 , A01G1/00 , A01N37/18 , A01H5/10
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N65/00 , A01N2300/00
摘要： The subject invention relates in part to stacking a Cry IAb protein and a Cry2Aa protein to make plants (particularly corn or maize) more durable and less prone to allowing insects to develop that are resistant to the activity of either of these two toxins. These stacks can be used to specifically target European cornborer.
摘要翻译：本发明部分地涉及堆积Cry IAb蛋白和Cry2Aa蛋白以使植物（特别是玉米或玉米）更耐用，并且更不易于允许对这两种毒素中的任一种的活性具有抗性的昆虫发育。这些堆叠可用于专门针对欧洲玉米棒。

10. 发明申请

US20130007923A1 USE OF Cry1Da IN COMBINATION WITH Cry1Ca FOR MANAGEMENT OF RESISTANT INSECTS 有权
标题翻译：使用Cry1Da与Cry1Ca联合治疗耐药性
公开(公告)号：US20130007923A1
公开(公告)日：2013-01-03
申请号：US13516663
申请日：2010-12-16
申请人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
发明人： Thomas Meade , Kenneth Narva , Nicholas P. Storer , Joel J. Sheets , Aaron T. Woosley , Stephanie L. Burton
IPC分类号： A01H5/00 , C12N5/10 , A01C11/00 , A01N37/46 , A01G1/00 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N65/00
摘要： The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.
摘要翻译：本发明包括用于控制秋季粘虫鳞翅目昆虫的方法和植物，所述植物包含Cry1Da杀虫蛋白和Cry1Ca杀虫蛋白，以及包含该对蛋白质的其它蛋白质的各种组合，以延迟或预防昆虫的抗性发展。

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