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1. 发明申请

US20150211986A1 SUPER-RESOLUTION MICROSCOPY METHOD AND DEVICE 有权
标题翻译：超分辨率显微镜方法和器件
公开(公告)号：US20150211986A1
公开(公告)日：2015-07-30
申请号：US14411373
申请日：2012-10-22
申请人： ZHEJIANG UNIVERSITY
发明人： Cuifang Kuang , Shuai Li , Xiang Hao , Zhaotai Gu , Xu Liu
IPC分类号： G01N21/27 , G02B21/00 , G01N21/25
CPC分类号： G01N21/27 , G01N21/255 , G01N21/6458 , G01N2201/06113 , G01N2201/064 , G02B21/002 , G02B21/0032 , G02B21/0068 , G02B27/58
摘要： This invention discloses a super-resolution microscopy method and device, of which the method comprises the following steps: converting laser beam into linearly polarized light after collimation; linearly polarized light is deflected and phase modulated by a spatial light modulator; the deflected beam is focused, collimated and then converted into circularly polarized light for projection on the sample to collect signal light from various scanning points on the sample, and obtaining the first signal light intensity; switching over modulation function to project linearly polarized light modulated by the second phase modulation on the sample to collect signal light from various scanning points on the sample, and obtaining the second signal light intensity; calculating valid signal light intensity to obtain the super-resolution image. This device features in a simple structure and easy operation, which can obtain a super-resolution beyond diffraction limit at a lower luminous power; it is quick in image formation with the frame frequency over 15 frames when the number of scanning points in each image is 512×512 .
摘要翻译：本发明公开了一种超分辨率显微镜方法和装置，其方法包括以下步骤：准直后将激光束转换成线偏振光; 线性偏振光被空间光调制器偏转和相位调制; 偏转光束被聚焦，准直，然后转换成圆偏振光，用于投射在样品上，以从样品上的各个扫描点收集信号光，并获得第一信号光强度; 切换调制功能，将样品上的第二相位调制调制的线偏振光投射到样品上的各个扫描点，以获得第二信号光强度; 计算有效信号光强度以获得超分辨率图像。该器件结构简单，操作方便，可以在较低的发光功率下获得超过衍射极限的超分辨率; 当每个图像中的扫描点数量为512×512时，帧频超过15帧的图像形成速度很快。

2. 发明授权

US09568417B2 Super-resolution microscopy method and device 有权
标题翻译：超分辨率显微镜方法和装置
公开(公告)号：US09568417B2
公开(公告)日：2017-02-14
申请号：US14411373
申请日：2012-10-22
申请人： ZHEJIANG UNIVERSITY
发明人： Cuifang Kuang , Shuai Li , Xiang Hao , Zhaotai Gu , Xu Liu
IPC分类号： G01J4/00 , G01N21/27 , G02B21/00 , G01N21/64 , G01N21/25 , G02B27/58
CPC分类号： G01N21/27 , G01N21/255 , G01N21/6458 , G01N2201/06113 , G01N2201/064 , G02B21/002 , G02B21/0032 , G02B21/0068 , G02B27/58
摘要： This invention discloses a super-resolution microscopy method and device, of which the method comprises the following steps: converting laser beam into linearly polarized light after collimation; linearly polarized light is deflected and phase modulated by a spatial light modulator; the deflected beam is focused, collimated and then converted into circularly polarized light for projection on the sample to collect signal light from various scanning points on the sample, and obtaining the first signal light intensity; switching over modulation function to project linearly polarized light modulated by the second phase modulation on the sample to collect signal light from various scanning points on the sample, and obtaining the second signal light intensity; calculating valid signal light intensity to obtain the super-resolution image. This device features in a simple structure and easy operation, which can obtain a super-resolution beyond diffraction limit at a lower luminous power; it is quick in image formation with the frame frequency over 15 frames when the number of scanning points in each image is 512×512.
摘要翻译：本发明公开了一种超分辨率显微镜方法和装置，其方法包括以下步骤：准直后将激光束转换成线偏振光; 线性偏振光被空间光调制器偏转和相位调制; 偏转光束被聚焦，准直，然后转换成圆偏振光，用于投射在样品上，以从样品上的各个扫描点收集信号光，并获得第一信号光强度; 切换调制功能，将样品上的第二相位调制调制的线偏振光投射到样品上的各个扫描点的信号光，并获得第二信号光强度; 计算有效信号光强度以获得超分辨率图像。该器件结构简单，操作方便，可以在较低的发光功率下获得超过衍射极限的超分辨率; 当每个图像中的扫描点数量为512×512时，帧频超过15帧的图像形成速度很快。

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