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1. 发明授权

US09096878B2 Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof 有权
标题翻译：用于使用动物细胞大量生产来自外来基因的蛋白质的表达载体及其用途
公开(公告)号：US09096878B2
公开(公告)日：2015-08-04
申请号：US13140905
申请日：2009-12-22
申请人： Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
发明人： Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
IPC分类号： C12P21/02 , C12N15/85
CPC分类号： C12P21/02 , C12N15/85 , C12N2830/00
摘要： The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译：本发明人进行了专门研究并成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的二氢叶酸还原酶基因顺反子，其表达已通过将密码子改变为最少常用的哺乳动物密码子以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。

2. 发明申请

US20120122083A1 EXPRESSION VECTOR FOR PRODUCING PROTEIN DERIVED FROM FOREIGN GENE IN LARGE QUANTITY USING ANIMAL CELLS, AND USE THEREOF 有权
标题翻译：用动物细胞生产大量外源基因衍生蛋白的表达载体及其用途
公开(公告)号：US20120122083A1
公开(公告)日：2012-05-17
申请号：US13140905
申请日：2009-12-22
申请人： Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
发明人： Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
IPC分类号： C12P21/00 , C12Q1/68 , C12N15/85
CPC分类号： C12P21/02 , C12N15/85 , C12N2830/00
摘要： The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译：本发明人进行了专门研究并成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的二氢叶酸还原酶基因顺反子，其表达已通过将密码子改变为最少常用的哺乳动物密码子以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。

3. 发明申请

US20120059152A1 PROTEIN SUBSTANCE HAVING TRIPLE HELIX STRUCTURE AND MANUFACTURING METHOD THEREFOR 有权
标题翻译：具有三重螺旋结构的蛋白质物质及其制造方法
公开(公告)号：US20120059152A1
公开(公告)日：2012-03-08
申请号：US13141138
申请日：2009-12-22
申请人： Yusuke Suzuki , Hiroshi Tahara , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
发明人： Yusuke Suzuki , Hiroshi Tahara , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
IPC分类号： C07K14/78 , C12P21/06
CPC分类号： C07K14/78 , C07K14/4726 , C07K2319/00 , C07K2319/02
摘要： As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要翻译：作为专门研究的结果，本发明人已经成功地发明了一种胶原基因构建体，其可以容易地纯化并保持与天然存在的胶原蛋白相当的三重螺旋结构，同时具有低分子量。具体地，通过亲和纯化进行一步纯化，因为CR-D（信号肽）具有碳水化合物识别结构域。通过将本发明的人胶原结构基因的一部分用MBL的胶原样结构基因部分代替，可以以高纯度获得维持三螺旋结构并且是热稳定的低分子量胶原，大批量。

4. 发明授权

US08470555B2 Protein substance having triple helix structure and manufacturing method therefor 有权
标题翻译：具有三螺旋结构的蛋白质物质及其制造方法
公开(公告)号：US08470555B2
公开(公告)日：2013-06-25
申请号：US13141138
申请日：2009-12-22
申请人： Yusuke Suzuki , Hiroshi Tahara , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
发明人： Yusuke Suzuki , Hiroshi Tahara , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
IPC分类号： C12P21/06 , C12N15/00 , C07H21/02 , C07K14/00
CPC分类号： C07K14/78 , C07K14/4726 , C07K2319/00 , C07K2319/02
摘要： As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要翻译：作为专门研究的结果，本发明人已经成功地发明了一种胶原基因构建体，其可以容易地纯化并保持与天然存在的胶原蛋白相当的三重螺旋结构，同时具有低分子量。具体地，通过亲和纯化进行一步纯化，因为CR-D（信号肽）具有碳水化合物识别结构域。通过将本发明的人胶原结构基因的一部分用MBL的胶原样结构基因部分代替，可以以高纯度获得维持三螺旋结构并且是热稳定的低分子量胶原，大批量。

5. 发明申请

US20110123993A1 EXPRESSION VECTOR FOR MASS PRODUCTION OF FOREIGN GENE-DERIVED PROTEIN USING ANIMAL CELL AND USE THEREOF 有权
标题翻译：使用动物细胞大量生产外源基因衍生蛋白的表达载体及其用途
公开(公告)号：US20110123993A1
公开(公告)日：2011-05-26
申请号：US12918776
申请日：2009-02-27
申请人： Yasuhiko Suzuki , Keiichi Yamamoto , Hiroshi Tahara , Yusuke Suzuki
发明人： Yasuhiko Suzuki , Keiichi Yamamoto , Hiroshi Tahara , Yusuke Suzuki
IPC分类号： C12N15/85 , C12P21/02 , C12Q1/68
CPC分类号： C12N15/85 , C12N9/1205 , C12N2830/50 , C12N2840/102 , C12Y207/01095
摘要： The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译：本发明人成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的药物抗性基因顺反子，其表达已通过将密码子改变为最不常用的密码子而被减弱哺乳动物以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。

6. 发明授权

US08653249B2 Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof 有权
标题翻译：用于使用动物细胞大规模生产外源基因衍生蛋白质的表达载体及其用途
公开(公告)号：US08653249B2
公开(公告)日：2014-02-18
申请号：US12918776
申请日：2009-02-27
申请人： Yasuhiko Suzuki , Keiichi Yamamoto , Hiroshi Tahara , Yusuke Suzuki
发明人： Yasuhiko Suzuki , Keiichi Yamamoto , Hiroshi Tahara , Yusuke Suzuki
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12N15/85 , C12N9/1205 , C12N2830/50 , C12N2840/102 , C12Y207/01095
摘要： The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译：本发明人成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的药物抗性基因顺反子，其表达已通过将密码子改变为最不常用的密码子而被减弱哺乳动物以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。

7. 发明授权

US09044983B2 Liquid jetting apparatus 有权
标题翻译：液体喷射装置
公开(公告)号：US09044983B2
公开(公告)日：2015-06-02
申请号：US13558489
申请日：2012-07-26
申请人： Mizuyo Takebayashi , Shohei Koide , Yusuke Suzuki , Hiromitsu Mizutani , Atsushi Ito
发明人： Mizuyo Takebayashi , Shohei Koide , Yusuke Suzuki , Hiromitsu Mizutani , Atsushi Ito
IPC分类号： B41J29/38 , B41J2/155
CPC分类号： B41J29/38 , B41J2/155 , B41J2202/20
摘要： A liquid jetting apparatus includes: a head unit including a liquid jetting head, a regulating member arranged to sandwich the head unit from a nozzle array direction, a restriction mechanism configured to restrict the head unit from displacement in an orthogonal direction perpendicular to the nozzle array direction, and a landing position correction mechanism configured to correct position of landing the liquid jetted from the plurality of nozzles onto the medium in the orthogonal direction. The restriction mechanism restricts a portion of the head unit inside of outmost nozzle arrays in the orthogonal direction.
摘要翻译：液体喷射装置包括：头单元，其包括液体喷射头，布置成从喷嘴阵列方向夹住头单元的限制构件;限制机构，其构造成限制头单元沿垂直于喷嘴阵列的垂直方向的位移方向和着陆位置校正机构，其配置为校正从多个喷嘴喷射的液体在正交方向上的位置。限制机构限制最外侧喷嘴阵列内的头部单元的正交方向的一部分。

8. 发明授权

US08851633B2 Liquid ejection apparatus 有权
标题翻译：液体喷射装置
公开(公告)号：US08851633B2
公开(公告)日：2014-10-07
申请号：US13362198
申请日：2012-01-31
申请人： Tomoyuki Kubo , Atsushi Ito , Yusuke Suzuki , Yasuo Kato
发明人： Tomoyuki Kubo , Atsushi Ito , Yusuke Suzuki , Yasuo Kato
IPC分类号： B41J2/05
CPC分类号： B41J2/14209 , B41J2002/14491 , B41J2202/08
摘要： A liquid ejection apparatus including: a head having: nozzles arranged in a nozzle-row direction; and ejection-energy applying portions; a drive IC for driving the ejection-energy applying portions; a control circuit board for controlling the drive IC; a wiring member on which the drive IC is mounted; and a support member for supporting the wiring member. The wiring member extends toward the control circuit board from a connection portion thereof connected to the ejection-energy applying portions, the wiring member having a first wiring portion extending in a first direction intersecting the nozzle-row direction and a second wiring portion extending in a second direction intersecting the first direction. The support member supports at least one of the first wiring portion and the second wiring portion, the at least one being located between the ejection-energy applying portions and the control circuit board.
摘要翻译：一种液体喷射装置，包括：头部，其具有：沿喷嘴行方向排列的喷嘴; 和喷射能量施加部分; 用于驱动喷射能量施加部分的驱动IC; 用于控制驱动IC的控制电路板; 其上安装有驱动IC的布线构件; 以及用于支撑所述配线构件的支撑构件。配线部件从与喷射能量施加部连接的连接部向控制电路基板延伸，配线部件具有沿与喷嘴列方向交叉的第一方向延伸的第一配线部，第二方向与第一方向相交。支撑构件支撑第一布线部分和第二布线部分中的至少一个，至少一个位于喷射能量施加部分和控制电路板之间。

9. 发明授权

US08831856B2 Control apparatus for internal combustion engine using estimated quantity of heat generated 有权
标题翻译：用于内燃机的控制装置，使用估计的发热量
公开(公告)号：US08831856B2
公开(公告)日：2014-09-09
申请号：US13002228
申请日：2010-04-19
申请人： Hiromichi Yasuda , Yusuke Suzuki , Soichiro Tanaka
发明人： Hiromichi Yasuda , Yusuke Suzuki , Soichiro Tanaka
IPC分类号： F02D28/00
CPC分类号： F02D35/028 , F02D35/024 , F02D41/0255 , F02D41/1498
摘要： An apparatus for controlling an internal combustion engine that can estimate a quantity of heat generated is provided.An arithmetic processing unit 20 can calculate PVκ variable according to a crank angle θ and dPVκ/dθ as a rate of change in PVκ. For convenience' sake, a “crank angle at which dPVκ/dθ is a maximum while PVκ is increasing” is to mean a “crank angle at a combustion proportion of 50%” and be referred to also as “θCA50”. PVκ calculated for θCA50 is to be referred to also as “PVκCA50”. In addition, for convenience' sake, a difference between PVκ (which is zero in the embodiment as shown in FIGS. 3 and 4) and PVκCA50 at a start of combustion is also referred to as ΔPVκCA50. A total quantity of heat generated Q is assumed to be twice as much as a value of ΔPVκCA50.
摘要翻译：提供了一种用于控制可以估计产生的热量的内燃机的装置。算术处理单元20可以计算PV＆kgr; 根据曲柄角度可变; 和dPV＆kgr; / d＆thetas; 作为PV＆kgr的变化率。为方便起见，一个“曲轴角度dPV＆kgr; / d＆thetas; 是最大值，而PV＆kgr; 正在增加“是指”燃烧比例为50％的曲柄角“，也称为”＆theta; CA50“。 PV＆kgr 计算为＆thetas; CA50也被称为“PV＆kgr; CA50”。另外为了方便起见，PV＆kgr之间的区别（在图3和图4所示的实施例中为零），燃烧开始时的PV＆kgr; CA50也被称为＆Dgr; PV＆kgr; CA50。产生的总热量Q假定为＆Dgr; PV＆kgr; CA50值的两倍。

10. 发明授权

US08721854B2 Control device for exhaust gas sensor of internal-combustion engine 有权
标题翻译：内燃机排气传感器控制装置
公开(公告)号：US08721854B2
公开(公告)日：2014-05-13
申请号：US10880554
申请日：2004-07-01
申请人： Keiichiro Aoki , Yusuke Suzuki , Yoshihiro Sakayanagi
发明人： Keiichiro Aoki , Yusuke Suzuki , Yoshihiro Sakayanagi
IPC分类号： G01N27/00
CPC分类号： G01N27/4065
摘要： Disclosed is a control device, which controls an air-fuel ratio sensor that is mounted in an exhaust path of an internal-combustion engine. The air-fuel ratio sensor is capable of pumping oxygen in a gas. Normally (time t0-t1, time t3 or later), a positive voltage Vp1 is applied to a sensor element (FIG. 7A), and the air-fuel ratio is calculated (FIG. 7C) in accordance with a sensor current (FIG. 7B). A heater is driven after internal-combustion engine startup to heat the sensor element. In a process in which the sensor element temperature rises, a negative voltage Vm, which is oriented in a direction different from that of the positive voltage Vp1, is applied to the sensor element.
摘要翻译：公开了一种控制装置，其控制安装在内燃机的排气路径中的空燃比传感器。空燃比传感器能够抽取气体中的氧气。通常（时间t0-t1，时间t3或更晚），向传感器元件施加正电压Vp1（图7A），并根据传感器电流计算空燃比（图7C）（图7C） 7B）。在内燃机起动之后，加热器被驱动以加热传感器元件。在传感器元件温度上升的过程中，将向与正电压Vp1不同的方向取向的负电压Vm施加到传感器元件。

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