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1. 发明申请

US20160145586A1 AAD-1 EVENT DAS-40278-9, RELATED TRANSGENIC CORN LINES, AND EVENT-SPECIFIC IDENTIFICATION THEREOF 审中-公开
标题翻译： AAD-1事件DAS-40278-9，相关转基因线及其事件特异性鉴定
公开(公告)号：US20160145586A1
公开(公告)日：2016-05-26
申请号：US14989787
申请日：2016-01-06
申请人： Yunxing Cory Cui , Jill Bryan , Donald Maum , Greg Gilles , Terry Wright , Jennifer Hamilton , Nicole Arnold , Nathan Vanopdorp , Tina Kaiser , Ning Zhou
发明人： Yunxing Cory Cui , Jill Bryan , Donald Maum , Greg Gilles , Terry Wright , Jennifer Hamilton , Nicole Arnold , Nathan Vanopdorp , Tina Kaiser , Ning Zhou
IPC分类号： C12N9/02 , C12N15/82 , A01H5/10 , C12Q1/68
CPC分类号： A01H5/10 , C12N9/0069 , C12N9/0071 , C12N15/8213 , C12N15/8274 , C12Q1/6895 , C12Q2600/16 , C12Y113/00
摘要： This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.
摘要翻译：本发明部分涉及植物育种和除草剂耐受性植物。本发明包括在玉米植物中的新型aad-1转化事件，其包含插入到玉米细胞基因组内特定位点的本文所述的多核苷酸序列。在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。另外，本发明提供了用于检测样品中（例如玉米粒）的受试者事件的存在的测定法。测定可以基于重组构建体的DNA序列，插入到玉米基因组中，并且基于插入位点侧翼的基因组序列。还提供了用于进行测定的试剂盒和条件。

2. 发明申请

US20150344903A1 Novel Herbicide Resistance Genes 审中-公开
标题翻译：新型除草剂抗性基因
公开(公告)号：US20150344903A1
公开(公告)日：2015-12-03
申请号：US14820893
申请日：2015-08-07
申请人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
发明人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
IPC分类号： C12N15/82
CPC分类号： C12N15/8274 , C12N9/0069 , C12N15/8209
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
摘要翻译：本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和改善除草剂抗性管理方案。更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。本发明还包括控制杂草的相关方法。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

3. 发明授权

US07838733B2 Herbicide resistance genes 有权
标题翻译：除草剂抗性基因
公开(公告)号：US07838733B2
公开(公告)日：2010-11-23
申请号：US11587893
申请日：2005-05-02
申请人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
发明人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
IPC分类号： C12N15/82 , C12N15/52
CPC分类号： C12N15/8274 , C12N9/0069 , C12N15/8209
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
摘要翻译：本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和改善除草剂抗性管理方案。更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。本发明还包括控制杂草的相关方法。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

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