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1. 发明申请

US20070087421A1 Transformant and process for producing polyester using the same 审中-公开
标题翻译：使用该方法生产聚酯的转化剂和方法
公开(公告)号：US20070087421A1
公开(公告)日：2007-04-19
申请号：US10554934
申请日：2004-05-14
申请人： Yuji Okubo , Tetsuya Nagaoka , Satoru Yokomizo , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
发明人： Yuji Okubo , Tetsuya Nagaoka , Satoru Yokomizo , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
IPC分类号： C12P7/62 , C12N9/18 , C07H21/04 , C12N15/74 , C12N1/21
CPC分类号： C12N9/1025 , C12P7/625
摘要： A gene expression cassette comprising an Aeromonas caviae-origin polyhydroxyalkanoic acid (PHA) synthase gene having a mutation introduced thereinto and a promoter and a terminator acting in a yeast; a transformant constructed by transferring the above gene expression cassette into a yeast; and a process for producing a polyester using the above transformant. Thus, it becomes possible to efficiently produce a copolymerized polyester having a high biodegradability and excellent properties by copolymerizing 3-hydroxyalkanoic acids in a yeast.
摘要翻译：一种基因表达盒，其包含导入其中的突变的气单胞菌来源的聚羟基链烷酸（PHA）合酶基因和启动子和在酵母中起作用的终止子; 通过将上述基因表达盒转移到酵母中构建的转化体; 以及使用上述转化体生产聚酯的方法。因此，通过在酵母中共聚3-羟基链烷酸，能够有效地制造具有高生物降解性和优异性能的共聚聚酯。

2. 发明授权

US07083972B2 Transformant and process for producing polyester by using the same 失效
标题翻译：通过使用它们来生产聚酯的转化体和方法
公开(公告)号：US07083972B2
公开(公告)日：2006-08-01
申请号：US10019543
申请日：2001-05-18
申请人： Satoru Yokomizo , Takeshi Fukuchi , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
发明人： Satoru Yokomizo , Takeshi Fukuchi , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
IPC分类号： C12N1/15 , C12N9/10 , C12N9/88
CPC分类号： C12N9/93 , C12N9/88 , C12N15/52 , C12P7/625
摘要： The present invention relates to a gene coding for a copolyester-synthesizing enzyme, a microorganism which utilizes the gene for the fermentative synthesis of a polyester, and a method of producing a polyester with the aid of the microorganism. More particularly, the present invention relates to a gene which functions in a host organism and, by an enzyme, synthesizes a plastic-like polymer degradable under the action of microorganisms in the natural environment (the soil, river or sea). The present invention also, more particularly, relates to a transformant derived from the host organism by transformation with the gene and having an improved ability to fermentatively synthesize a plastic-like polymer, and a method of producing a copolyester with the aid of the transformant.
摘要翻译：本发明涉及编码共聚酯合成酶的基因，利用该基因发酵合成聚酯的微生物，以及借助于微生物生产聚酯的方法。更具体地，本发明涉及在宿主生物体中起作用的基因，并且通过酶合成在天然环境（土壤，河流或海洋）中在微生物的作用下可降解的塑料状聚合物。本发明还特别涉及通过用该基因转化并具有改进的发酵合成塑料状聚合物的能力的来自宿主生物的转化体，以及借助于转化体制备共聚酯的方法。

3. 发明授权

US07429469B2 Enzyme gene participating in the synthesis of polyester and process for producing polyester using the same 失效
标题翻译：参与聚酯合成的酶基因及其制备方法
公开(公告)号：US07429469B2
公开(公告)日：2008-09-30
申请号：US10491498
申请日：2002-10-09
申请人： Satoru Yokomizo , Takeshi Fukuchi , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
发明人： Satoru Yokomizo , Takeshi Fukuchi , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
IPC分类号： C07H21/04 , C12P7/62 , C12N15/74 , C12N1/21
CPC分类号： C07K14/40 , C07K2319/01 , C12N9/88 , C12N15/52 , C12P7/625
摘要： This invention relates to a method of producing polyesters resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) and having biodegradability and good physical properties using yeasts as hosts. By constructing at least one enzyme gene involved in polyester synthesis by adding a DNA coding a peroxisome-targeting signal, introducing an enzyme gene expression cassette containing that gene into yeast, and cultivating the thus-obtained transformant, it becomes possible to cause accumulation of a polyester resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) in yeast cells and recover the polyester from the culture.
摘要翻译：本发明涉及由使用酵母作为宿主的3-羟基烷酸的均聚或共聚而具有生物降解性和良好物理性能的聚酯的制造方法。通过添加编码过氧化物酶体靶向信号的DNA构建至少一个参与聚酯合成的酶基因，将含有该基因的酶基因表达盒导入酵母中，培养由此得到的转化体，可以使由酵母细胞中的3-羟基链烷酸的均聚或共聚得到的聚酯，并从培养物中回收聚酯。

4. 发明申请

US20080044878A1 Novel Promoters 审中-公开
标题翻译：小说推广者
公开(公告)号：US20080044878A1
公开(公告)日：2008-02-21
申请号：US10505229
申请日：2003-04-08
申请人： Tetsuya Nagaoka , Satoru Yokomizo , Kenji Miyamoto , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ota
发明人： Tetsuya Nagaoka , Satoru Yokomizo , Kenji Miyamoto , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ota
IPC分类号： C07H21/04 , C12N1/00 , C12P1/02
CPC分类号： C07K14/4716 , C12N9/1025 , C12N15/815
摘要： The present invention provides a production method of a copolymeric polyester which comprises culturing an ACT1 gene promoter shown under SEQ ID NO: 9, a GAP3 gene promoter shown under SEQ ID NO: 10; a PMA1 gene promoter shown under SEQ ID NO: 11, and, a TEF1 gene promoter shown under SEQ ID NO: 12; a plasmid which contains the gene expression unit comprising said promoter; a transformed cell as resulting from transformation of the said plasmid; and said transformed cell.
摘要翻译：本发明提供共聚聚酯的制造方法，其包括培养SEQ ID NO：9所示的ACT1基因启动子，SEQ ID NO：10所示的GAP3基因启动子; SEQ ID NO：11所示的PMA1基因启动子和SEQ ID NO：12所示的TEF1基因启动子; 含有包含所述启动子的基因表达单元的质粒; 由所述质粒转化产生的转化细胞; 和转化细胞。

5. 发明申请

US20080233620A1 Novel Transformant and Process for Producing Polyester Using the Same 审中-公开
标题翻译：用于生产聚酯的新型转化体和方法
公开(公告)号：US20080233620A1
公开(公告)日：2008-09-25
申请号：US10591248
申请日：2005-03-03
申请人： Yuji Okubo , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
发明人： Yuji Okubo , Keiji Matsumoto , Masamichi Takagi , Akinori Ohta
IPC分类号： C12N15/74 , C12N1/00 , C12P1/00 , C12P7/62
CPC分类号： C12N9/1025 , C12N9/0006 , C12N9/1096 , C12N9/88 , C12N9/93 , C12P7/625
摘要： The present invention provides a process for producing yeast excellent in cell productivity and gene manipulation of which is easy, being added with nutritional requirement by disrupting only a specific gene, and a transformant thereof. Moreover, the present invention also provides a process for producing a gene expression product, particularly a polyhydroxyalkanoic acid.In the present invention, yeast in which a plurality of genes is disrupted is produced using the homologous recombination. Moreover, a transformant is obtained by introducing a plurality of enzyme genes involved with polyhydroxyalkanoic acid synthesis such as a polyhydroxyalkanoic acid synthase gene and an acetoacetyl CoA reductase gene into said gene-disrupted yeast. Furthermore, said transformant is cultured, copolyesters comprising a polyhydroxyalkanoic acid are efficiently accumulated within the cells, and a polymer is harvested from the cultured product.
摘要翻译：本发明提供一种生产酵母的方法，其转基因操作容易，基因操纵简单，营养需要通过仅破坏特定基因而产生转化体。此外，本发明还提供了生产基因表达产物，特别是聚羟基链烷酸的方法。在本发明中，使用同源重组产生多个基因被破坏的酵母。此外，通过将涉及聚羟基链烷酸合成的多种酶基因如聚羟基链烷酸合酶基因和乙酰乙酰辅酶A还原酶基因引入所述基因破坏的酵母中而获得转化体。此外，培养所述转化体，包含聚羟基链烷酸的共聚酯有效地积聚在细胞内，并从培养产物中收获聚合物。

6. 发明申请

US20060148049A1 Gene disrupted yeast 审中-公开
标题翻译：基因破坏酵母
公开(公告)号：US20060148049A1
公开(公告)日：2006-07-06
申请号：US10250297
申请日：2002-01-18
申请人： Takeshi Fukuchi , Satoru Yokomizo , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ota
发明人： Takeshi Fukuchi , Satoru Yokomizo , Fumio Osakada , Keiji Matsumoto , Masamichi Takagi , Akinori Ota
IPC分类号： C12P7/62 , C12N15/74 , C12N1/18
CPC分类号： C12N9/93 , C12P7/625 , C12Y603/02006
摘要： The invention provides a yeast in which a specific gene alone has been disrupted and to which auxotrophy is impared to construct a system for producing an industrially useful substance through gene recombination by utilizing characteristics of a yeast. In the practice of the invention, a yeast strain in which ADE1 gene has been disrupted is constructed by homologous recombination with a chromosomal DNA by using an ADE1 gene fragment encoding phosphoribosylaminoimidazole succinocarboxamide synthase (EC6.3.2.6) of Candida maltosa. In the practice of the invention, then, this disrupted strain is transformed by a gene expression cassette containing a biodegradable polyester synthase gene and the obtained transformant is cultured to thereby produce biodegradable polyester.
摘要翻译：本发明提供了一种酵母，其中单独的特定基因已经被破坏并且通过利用酵母的特性通过基因重组构建了用于产生工业上有用的物质的制剂。在本发明的实践中，通过使用编码奇异假丝酵母的磷酸核糖基氨基咪唑琥珀酰胺合成酶（EC6.3.2.6）的ADE1基因片段，通过与染色体DNA的同源重组来构建其中ADE1基因被破坏的酵母菌株。在本发明的实践中，通过含有可生物降解的聚酯合酶基因的基因表达盒转化该破坏的菌株，培养得到的转化体，从而生成可生物降解的聚酯。

7. 发明授权

US5407819A Human plasminogen activator variants having amino acids 37-42 substituted and a method for their manufacture 失效
标题翻译：具有37-42位氨基酸的人纤溶酶原激活物变体及其制备方法
公开(公告)号：US5407819A
公开(公告)日：1995-04-18
申请号：US869380
申请日：1992-04-16
申请人： Hitoshi Yahara , Tetsuya Nagaoka , Kazuyoshi Yajima , Yasuhiro Inenaka , Keiji Matsumoto , Tetsu Kakutani
发明人： Hitoshi Yahara , Tetsuya Nagaoka , Kazuyoshi Yajima , Yasuhiro Inenaka , Keiji Matsumoto , Tetsu Kakutani
IPC分类号： C12N9/72 , C12N15/58 , C12N5/00 , C12N15/00 , A61K37/547
CPC分类号： C12N9/6459 , C12Y304/21069
摘要： A TPA variant with replacement of amino acids is provided. In a typical embodiment, the variant has an amino acid sequence with replacement of the amino acid group consisting of asparagine at the 37th position, serine at the 38th position, glycine at the 39th position, arginine at the 40th position, alanine at the 41st position, and glutamine at the 42nd position from the N-terminus of the amino acid sequence of mature human tissue plasminogen activator.
摘要翻译：提供了具有替换氨基酸的TPA变体。在典型的实施方案中，变体具有氨基酸序列，其替换由第37位天冬酰胺，第38位丝氨酸，第39位甘氨酸，第40位精氨酸，第41位丙氨酸组成的氨基酸组，和谷氨酰胺在成熟的人组织纤溶酶原激活物的氨基酸序列的N末端的第42位。

8. 发明授权

US08904639B2 Method of producing liquid ejection head 有权
标题翻译：液体喷射头的制造方法
公开(公告)号：US08904639B2
公开(公告)日：2014-12-09
申请号：US13223066
申请日：2011-08-31
申请人： Keiji Matsumoto , Shuji Koyama , Sakai Yokoyama , Kenji Fujii , Jun Yamamuro
发明人： Keiji Matsumoto , Shuji Koyama , Sakai Yokoyama , Kenji Fujii , Jun Yamamuro
IPC分类号： B21D53/76 , B23P17/00 , B41J2/135 , B41J2/16
CPC分类号： B41J2/1603 , B41J2/1606 , B41J2/1628 , B41J2/1629 , B41J2/1632 , B41J2/1634 , Y10T29/49401
摘要： Disclosed is a method of including the steps of preparing a substrate having a flow-path-wall member; bonding the flow-path-wall member to a resin layer that is composed of a photo-curing resin and serves as the ejection port member such that spaces serving as the flow paths are provided between the substrate and the photo-curing resin; providing through-holes in the resin layer such that the spaces communicate with the outside air; exposing part of the resin layer to light to form an exposed portion and an unexposed portion; heating the exposed portion of the resin layer; and removing the unexposed portion from the heated resin layer to form the ejection ports, removing the unexposed portion from the heated resin layer to form the ejection ports, thereby forming the ejection port member.
摘要翻译：公开了一种包括制备具有流路壁构件的基板的步骤的方法，将流路壁构件结合到由光固化树脂构成的树脂层，并且用作喷射口构件，使得在基板和光固化树脂之间设置用作流路的空间; 在树脂层中设置通孔，使得空间与外部空气连通; 将树脂层的一部分暴露于光以形成暴露部分和未曝光部分; 加热树脂层的暴露部分; 从被加热的树脂层去除未曝光部分以形成喷射口，从被加热的树脂层去除未曝光部分以形成喷射口，从而形成喷射口部件。

9. 发明授权

US08703509B2 Method for manufacturing a substrate for liquid-ejecting heads and a liquid-ejecting head 失效
标题翻译：喷液头用基材和喷液头的制造方法
公开(公告)号：US08703509B2
公开(公告)日：2014-04-22
申请号：US12973708
申请日：2010-12-20
申请人： Hiroyuki Abo , Keiji Watanabe , Keiji Matsumoto
发明人： Hiroyuki Abo , Keiji Watanabe , Keiji Matsumoto
IPC分类号： H01L21/311 , H01L21/302
CPC分类号： B41J2/1603 , B41J2/1629
摘要： A method for manufacturing a substrate for liquid-ejecting heads includes etching a surface of a silicon substrate using a first etchant, with a silicon oxide layer as a mask, to form a depression as a part of a liquid supply port, and subsequently etching at least the silicon oxide layer and the thickness sandwiched between the depression and the etched surface of the silicon substrate with a second etchant to form the liquid supply port.
摘要翻译：一种液体喷射头用基板的制造方法，其特征在于，使用第一蚀刻剂，以氧化硅层作为掩模蚀刻硅基板的表面，形成作为液体供给口的一部分的凹部，用第二蚀刻剂将硅氧化物层和夹在硅衬底的蚀刻表面之间的厚度最小化，以形成液体供应端口。

10. 发明授权

US08630770B2 Vehicle and feedback control method 有权
标题翻译：车辆和反馈控制方法
公开(公告)号：US08630770B2
公开(公告)日：2014-01-14
申请号：US13264805
申请日：2010-03-11
申请人： Keiji Matsumoto , Jun Terashima
发明人： Keiji Matsumoto , Jun Terashima
IPC分类号： G06F7/00
CPC分类号： B62D7/1509 , B60L15/2036 , B60L2200/22 , B62D6/002 , Y02T10/645 , Y02T10/72 , Y02T10/7275
摘要： A vehicle capable of easily translating in an arbitrary direction. A vehicle comprising: wheels composed of two front wheels and two rear wheels; four steering actuators connected to the wheels, respectively, and steering the wheels; four drive actuators connected to the wheels, respectively, and driving the wheels; a joystick (64) tiltable in an arbitrary direction to perform operation and instructing the operation of the steering actuators; an accelerator pedal (63) for instructing the operation of the drive actuators; and a controller (100) which, when the joystick (64) is tilted, steers the wheels in the direction in which the joystick (64) is tilted, and when the accelerator pedal (63) is operated, drives the wheels on the basis of the amount of the operation of the accelerator pedal (63).
摘要翻译：能够容易地在任意方向平移的车辆。一种车辆，包括：由两个前轮和两个后轮组成的车轮; 分别连接到车轮的四个转向致动器并且转向车轮; 四个驱动致动器分别连接到车轮并驱动车轮; 操纵杆（64），其可在任意方向上倾斜，以执行操作并指示转向致动器的操作; 用于指示驱动致动器的操作的加速器踏板（63）以及控制器（100），当操纵杆（64）倾斜时，使操纵杆（64）倾斜的方向转动车轮，并且当加速器踏板（63）被操作时，驱动车轮的加速踏板（63）的操作量。

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