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1. 发明授权

US6103528A Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices 失效
标题翻译：用于三维基质体外细胞培养的可逆胶凝培养基
公开(公告)号：US6103528A
公开(公告)日：2000-08-15
申请号：US62483
申请日：1998-04-17
申请人： Yuehuei H. An , Vladimir A. Mironov , Anna Gutowska
发明人： Yuehuei H. An , Vladimir A. Mironov , Anna Gutowska
IPC分类号： C12N5/00 , C12N5/08 , C12N5/06 , C12N11/04
CPC分类号： C12N5/0068 , C12N2533/30 , C12N2533/54 , C12N2539/10
摘要： A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.
摘要翻译：通过将丙烯酰胺衍生物与亲水性共聚单体共聚合形成可以形成多条直链形式的可逆（优选热可逆）胶凝线性无规共聚物，可制备用于形成用于体外细胞培养的三维基质的胶凝细胞培养基具有大于或等于最小胶凝分子量截留值的多个分子量，将该共聚物与水性溶剂混合以形成可逆胶凝溶液，并将细胞培养基加入到胶凝溶液中以形成胶凝细胞培养基。将细胞如软骨细胞或肝细胞加入到培养基中以形成接种的培养基，并培养培养基的温度以凝固接种的培养基并形成含有细胞的三维基质。在细胞繁殖后，通过降低温度以溶解基质并离心，可以回收细胞。

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